Construction and immunogenicity of recombinant Mycobacterium bovis BCG expressing GP5 and M protein of porcine reproductive respiratory syndrome virus
Mycobacterium bovis BCG was used to express a truncated form of GP5 (lacking the first 30 NH 2-terminal residues) and M protein of porcine reproductive and respiratory syndrome virus (PRRSV). The PRRSV proteins were expressed in BCG under control of the mycobacterial hsp60 gene promoter either in th...
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| Veröffentlicht in: | Vaccine 2002-11, Vol.21 (1), p.21-29 |
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| creator | Bastos, Reginaldo G Dellagostin, Odir A Barletta, Raúl G Doster, Allan R Nelson, Eric Osorio, Fernando A |
| description | Mycobacterium bovis BCG was used to express a truncated form of GP5 (lacking the first 30 NH
2-terminal residues) and M protein of porcine reproductive and respiratory syndrome virus (PRRSV). The PRRSV proteins were expressed in BCG under control of the mycobacterial
hsp60 gene promoter either in the mycobacterial cytoplasm (BCGGP5cyt and BCGMcyt) or as MT19-fusion proteins on the mycobacterial surface (BCGGP5surf and BCGMsurf). Mice inoculated with BCGGP5surf and BCGMsurf developed antibodies against the viral proteins at 30 days post-inoculation (dpi) as detected by ELISA and Western blot. By 60
dpi, the animals developed titer of neutralizing antibodies of 8. A PRRSV-specific gamma interferon response was also detected in splenocytes of recombinant BCG-inoculated mice at 60 and 90
dpi. These results indicate that BCG was able to express antigens of PRRSV and elicit an immune response against the viral proteins in mice. |
| doi_str_mv | 10.1016/S0264-410X(02)00443-7 |
| format | Article |
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2-terminal residues) and M protein of porcine reproductive and respiratory syndrome virus (PRRSV). The PRRSV proteins were expressed in BCG under control of the mycobacterial
hsp60 gene promoter either in the mycobacterial cytoplasm (BCGGP5cyt and BCGMcyt) or as MT19-fusion proteins on the mycobacterial surface (BCGGP5surf and BCGMsurf). Mice inoculated with BCGGP5surf and BCGMsurf developed antibodies against the viral proteins at 30 days post-inoculation (dpi) as detected by ELISA and Western blot. By 60
dpi, the animals developed titer of neutralizing antibodies of 8. A PRRSV-specific gamma interferon response was also detected in splenocytes of recombinant BCG-inoculated mice at 60 and 90
dpi. These results indicate that BCG was able to express antigens of PRRSV and elicit an immune response against the viral proteins in mice.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/S0264-410X(02)00443-7</identifier><identifier>PMID: 12443659</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animals ; Antibodies, Viral - immunology ; Biological and medical sciences ; Blotting, Western ; Cloning, Molecular ; double prime M protein ; Enzyme-Linked Immunosorbent Assay ; Fundamental and applied biological sciences. Psychology ; gamma -Interferon ; Gene Expression ; Genetic Vectors ; glycoprotein gp35 ; glycoprotein gp5 ; GP5 and M PRRSV protein ; HSP60 gene ; Mice ; Mice, Inbred BALB C ; Microbiology ; movement protein ; Mycobacterium bovis ; Mycobacterium bovis - genetics ; Peptides - chemical synthesis ; Peptides - chemistry ; Peptides - immunology ; Porcine respiratory and reproductive syndrome virus ; Porcine respiratory and reproductive syndrome virus - chemistry ; Porcine respiratory and reproductive syndrome virus - genetics ; Porcine respiratory and reproductive syndrome virus - isolation & purification ; PRRSV ; Recombinant BCG ; Recombinant Proteins - immunology ; Swine ; Vaccination ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies ; Vaccines, DNA - immunology ; Viral Envelope Proteins - genetics ; Viral Envelope Proteins - immunology ; Viral Matrix Proteins - genetics ; Viral Matrix Proteins - immunology ; Virology</subject><ispartof>Vaccine, 2002-11, Vol.21 (1), p.21-29</ispartof><rights>2002 Elsevier Science Ltd</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c573t-24fb6c172b7942c3d47edbf653d4296c66fe381c254651289d0f3de181263dec3</citedby><cites>FETCH-LOGICAL-c573t-24fb6c172b7942c3d47edbf653d4296c66fe381c254651289d0f3de181263dec3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0264410X02004437$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14029364$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12443659$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bastos, Reginaldo G</creatorcontrib><creatorcontrib>Dellagostin, Odir A</creatorcontrib><creatorcontrib>Barletta, Raúl G</creatorcontrib><creatorcontrib>Doster, Allan R</creatorcontrib><creatorcontrib>Nelson, Eric</creatorcontrib><creatorcontrib>Osorio, Fernando A</creatorcontrib><title>Construction and immunogenicity of recombinant Mycobacterium bovis BCG expressing GP5 and M protein of porcine reproductive respiratory syndrome virus</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Mycobacterium bovis BCG was used to express a truncated form of GP5 (lacking the first 30 NH
2-terminal residues) and M protein of porcine reproductive and respiratory syndrome virus (PRRSV). The PRRSV proteins were expressed in BCG under control of the mycobacterial
hsp60 gene promoter either in the mycobacterial cytoplasm (BCGGP5cyt and BCGMcyt) or as MT19-fusion proteins on the mycobacterial surface (BCGGP5surf and BCGMsurf). Mice inoculated with BCGGP5surf and BCGMsurf developed antibodies against the viral proteins at 30 days post-inoculation (dpi) as detected by ELISA and Western blot. By 60
dpi, the animals developed titer of neutralizing antibodies of 8. A PRRSV-specific gamma interferon response was also detected in splenocytes of recombinant BCG-inoculated mice at 60 and 90
dpi. These results indicate that BCG was able to express antigens of PRRSV and elicit an immune response against the viral proteins in mice.</description><subject>Animals</subject><subject>Antibodies, Viral - immunology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cloning, Molecular</subject><subject>double prime M protein</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gamma -Interferon</subject><subject>Gene Expression</subject><subject>Genetic Vectors</subject><subject>glycoprotein gp35</subject><subject>glycoprotein gp5</subject><subject>GP5 and M PRRSV protein</subject><subject>HSP60 gene</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>movement protein</subject><subject>Mycobacterium bovis</subject><subject>Mycobacterium bovis - genetics</subject><subject>Peptides - chemical synthesis</subject><subject>Peptides - chemistry</subject><subject>Peptides - immunology</subject><subject>Porcine respiratory and reproductive syndrome virus</subject><subject>Porcine respiratory and reproductive syndrome virus - chemistry</subject><subject>Porcine respiratory and reproductive syndrome virus - genetics</subject><subject>Porcine respiratory and reproductive syndrome virus - isolation & purification</subject><subject>PRRSV</subject><subject>Recombinant BCG</subject><subject>Recombinant Proteins - immunology</subject><subject>Swine</subject><subject>Vaccination</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Vaccines, DNA - immunology</subject><subject>Viral Envelope Proteins - genetics</subject><subject>Viral Envelope Proteins - immunology</subject><subject>Viral Matrix Proteins - genetics</subject><subject>Viral Matrix Proteins - immunology</subject><subject>Virology</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctu1DAUhi0EotPCI4C8AZVFwHZ8SVYIRmVAagUSILGzHOekMprYg-2MyIvwvDgzI7qjK1_0nf8c-0PoGSWvKaHyzVfCJK84JT8uCXtFCOd1pR6gFW1UXTFBm4do9Q85Q-cp_SSEiJq2j9EZZQWXol2hP-vgU46TzS54bHyP3ThOPtyCd9blGYcBR7Bh7Jw3PuOb2YbO2AzRTSPuwt4l_H69wfB7FyEl52_x5os4BN3gXQwZnF8ydiFa56Fklct-abdfDmnnoskhzjjNvo9hBLx3cUpP0KPBbBM8Pa0X6PuHq2_rj9X1582n9bvrygpV54rxoZOWKtapljNb91xB3w1SlB1rpZVygLqhlgkuBWVN25Oh7oE2lMmy2voCvTzmlql-TZCyHl2ysN0aD2FKWjFFmqbm94K0UJKRtoCX_wcFIUq0VC6oOKI2hpQiDHoX3WjirCnRi2R9kKwXg5owfZCsVal7fmoxdSP0d1UnqwV4cQJMsmY7ROOtS3ccJ6yt5fKot0cOyhfvHUSdrANvoXfFedZ9cPeM8hfJ8sZ2</recordid><startdate>20021122</startdate><enddate>20021122</enddate><creator>Bastos, Reginaldo G</creator><creator>Dellagostin, Odir A</creator><creator>Barletta, Raúl G</creator><creator>Doster, Allan R</creator><creator>Nelson, Eric</creator><creator>Osorio, Fernando A</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>7T7</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20021122</creationdate><title>Construction and immunogenicity of recombinant Mycobacterium bovis BCG expressing GP5 and M protein of porcine reproductive respiratory syndrome virus</title><author>Bastos, Reginaldo G ; Dellagostin, Odir A ; Barletta, Raúl G ; Doster, Allan R ; Nelson, Eric ; Osorio, Fernando A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c573t-24fb6c172b7942c3d47edbf653d4296c66fe381c254651289d0f3de181263dec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Antibodies, Viral - immunology</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Cloning, Molecular</topic><topic>double prime M protein</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gamma -Interferon</topic><topic>Gene Expression</topic><topic>Genetic Vectors</topic><topic>glycoprotein gp35</topic><topic>glycoprotein gp5</topic><topic>GP5 and M PRRSV protein</topic><topic>HSP60 gene</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>movement protein</topic><topic>Mycobacterium bovis</topic><topic>Mycobacterium bovis - genetics</topic><topic>Peptides - chemical synthesis</topic><topic>Peptides - chemistry</topic><topic>Peptides - immunology</topic><topic>Porcine respiratory and reproductive syndrome virus</topic><topic>Porcine respiratory and reproductive syndrome virus - chemistry</topic><topic>Porcine respiratory and reproductive syndrome virus - genetics</topic><topic>Porcine respiratory and reproductive syndrome virus - isolation & purification</topic><topic>PRRSV</topic><topic>Recombinant BCG</topic><topic>Recombinant Proteins - immunology</topic><topic>Swine</topic><topic>Vaccination</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Vaccines, DNA - immunology</topic><topic>Viral Envelope Proteins - genetics</topic><topic>Viral Envelope Proteins - immunology</topic><topic>Viral Matrix Proteins - genetics</topic><topic>Viral Matrix Proteins - immunology</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bastos, Reginaldo G</creatorcontrib><creatorcontrib>Dellagostin, Odir A</creatorcontrib><creatorcontrib>Barletta, Raúl G</creatorcontrib><creatorcontrib>Doster, Allan R</creatorcontrib><creatorcontrib>Nelson, Eric</creatorcontrib><creatorcontrib>Osorio, Fernando A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bastos, Reginaldo G</au><au>Dellagostin, Odir A</au><au>Barletta, Raúl G</au><au>Doster, Allan R</au><au>Nelson, Eric</au><au>Osorio, Fernando A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Construction and immunogenicity of recombinant Mycobacterium bovis BCG expressing GP5 and M protein of porcine reproductive respiratory syndrome virus</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2002-11-22</date><risdate>2002</risdate><volume>21</volume><issue>1</issue><spage>21</spage><epage>29</epage><pages>21-29</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Mycobacterium bovis BCG was used to express a truncated form of GP5 (lacking the first 30 NH
2-terminal residues) and M protein of porcine reproductive and respiratory syndrome virus (PRRSV). The PRRSV proteins were expressed in BCG under control of the mycobacterial
hsp60 gene promoter either in the mycobacterial cytoplasm (BCGGP5cyt and BCGMcyt) or as MT19-fusion proteins on the mycobacterial surface (BCGGP5surf and BCGMsurf). Mice inoculated with BCGGP5surf and BCGMsurf developed antibodies against the viral proteins at 30 days post-inoculation (dpi) as detected by ELISA and Western blot. By 60
dpi, the animals developed titer of neutralizing antibodies of 8. A PRRSV-specific gamma interferon response was also detected in splenocytes of recombinant BCG-inoculated mice at 60 and 90
dpi. These results indicate that BCG was able to express antigens of PRRSV and elicit an immune response against the viral proteins in mice.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>12443659</pmid><doi>10.1016/S0264-410X(02)00443-7</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Antibodies, Viral - immunology Biological and medical sciences Blotting, Western Cloning, Molecular double prime M protein Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology gamma -Interferon Gene Expression Genetic Vectors glycoprotein gp35 glycoprotein gp5 GP5 and M PRRSV protein HSP60 gene Mice Mice, Inbred BALB C Microbiology movement protein Mycobacterium bovis Mycobacterium bovis - genetics Peptides - chemical synthesis Peptides - chemistry Peptides - immunology Porcine respiratory and reproductive syndrome virus Porcine respiratory and reproductive syndrome virus - chemistry Porcine respiratory and reproductive syndrome virus - genetics Porcine respiratory and reproductive syndrome virus - isolation & purification PRRSV Recombinant BCG Recombinant Proteins - immunology Swine Vaccination Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vaccines, DNA - immunology Viral Envelope Proteins - genetics Viral Envelope Proteins - immunology Viral Matrix Proteins - genetics Viral Matrix Proteins - immunology Virology |
| title | Construction and immunogenicity of recombinant Mycobacterium bovis BCG expressing GP5 and M protein of porcine reproductive respiratory syndrome virus |
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