In vitro effects of β-carotene on human oral keratinocytes from precancerous lesions and squamous carcinoma
Human keratinocytes, obtained from bioptic specimens of healthy and preneoplastic oral mucosa, and from human cell lines from oral cavity tumors (KB and SCC-25) were treated with β-carotene (10 μM). The colony forming efficiency (CFE), the proliferation rate and the frequency of micronucleated cells...
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| Veröffentlicht in: | Anti-cancer drugs 1991-12, Vol.2 (6), p.581-590 |
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| creator | Toma, Salvatore Albanese, Enrico Palumbo, Raffaella Nicoló, Guido Morerio, Cristina Mangiante, Paolo Erasmo Bonatti, Stefania Cancedda, Ranieri Santi, Leonardo |
| description | Human keratinocytes, obtained from bioptic specimens of healthy and preneoplastic oral mucosa, and from human cell lines from oral cavity tumors (KB and SCC-25) were treated with β-carotene (10 μM). The colony forming efficiency (CFE), the proliferation rate and the frequency of micronucleated cells were measured in these cultures. CFE was significantly reduced (p < 0.05) by β-carotene treatment in cells from healthy mucosa and in KB cells. Decreases (p < 0.05; NS) were also observed in cells from pathological mucosa and in SCC-25 cells. Cell proliferation rate was not substantially affected by β-carotene in all cultures. Finally, a decreased frequency of micronucleated cells was found in treated cultures, but significant reductions (p < 0.05) were only observed in cultures from oral mucosa (healthy and pathological) as well as in KB cell cultures. Our results indicate that β-carotene is able to reduce the clonogenic activity (CFE), even if it does not seem to influence cell proliferation, and that it has a protective effect against genotoxic damage. |
| doi_str_mv | 10.1097/00001813-199112000-00009 |
| format | Article |
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The colony forming efficiency (CFE), the proliferation rate and the frequency of micronucleated cells were measured in these cultures. CFE was significantly reduced (p < 0.05) by β-carotene treatment in cells from healthy mucosa and in KB cells. Decreases (p < 0.05; NS) were also observed in cells from pathological mucosa and in SCC-25 cells. Cell proliferation rate was not substantially affected by β-carotene in all cultures. Finally, a decreased frequency of micronucleated cells was found in treated cultures, but significant reductions (p < 0.05) were only observed in cultures from oral mucosa (healthy and pathological) as well as in KB cell cultures. Our results indicate that β-carotene is able to reduce the clonogenic activity (CFE), even if it does not seem to influence cell proliferation, and that it has a protective effect against genotoxic damage.</description><identifier>ISSN: 0959-4973</identifier><identifier>EISSN: 1473-5741</identifier><identifier>DOI: 10.1097/00001813-199112000-00009</identifier><identifier>PMID: 1806035</identifier><language>eng</language><publisher>England: Lippincott-Raven Publishers</publisher><subject>Adolescent ; Adult ; Aged ; beta Carotene ; Biopsy ; Carcinoma, Squamous Cell - drug therapy ; Carcinoma, Squamous Cell - pathology ; Carcinoma, Squamous Cell - ultrastructure ; Carotenoids - pharmacology ; Cell Division - drug effects ; Cell Division - physiology ; Cells, Cultured ; Female ; Humans ; Keratinocytes - cytology ; Keratinocytes - drug effects ; Keratinocytes - ultrastructure ; Male ; Micronucleus Tests ; Middle Aged ; Mouth Neoplasms - drug therapy ; Mouth Neoplasms - pathology ; Mouth Neoplasms - ultrastructure ; Precancerous Conditions - drug therapy ; Precancerous Conditions - pathology ; Precancerous Conditions - ultrastructure ; Stem Cells - drug effects</subject><ispartof>Anti-cancer drugs, 1991-12, Vol.2 (6), p.581-590</ispartof><rights>Lippincott-Raven Publishers.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2709-bade427aeaff08d05ef0a664d23b2d5bbd97de8901351170c67a726658a3392a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1806035$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Toma, Salvatore</creatorcontrib><creatorcontrib>Albanese, Enrico</creatorcontrib><creatorcontrib>Palumbo, Raffaella</creatorcontrib><creatorcontrib>Nicoló, Guido</creatorcontrib><creatorcontrib>Morerio, Cristina</creatorcontrib><creatorcontrib>Mangiante, Paolo Erasmo</creatorcontrib><creatorcontrib>Bonatti, Stefania</creatorcontrib><creatorcontrib>Cancedda, Ranieri</creatorcontrib><creatorcontrib>Santi, Leonardo</creatorcontrib><title>In vitro effects of β-carotene on human oral keratinocytes from precancerous lesions and squamous carcinoma</title><title>Anti-cancer drugs</title><addtitle>Anticancer Drugs</addtitle><description>Human keratinocytes, obtained from bioptic specimens of healthy and preneoplastic oral mucosa, and from human cell lines from oral cavity tumors (KB and SCC-25) were treated with β-carotene (10 μM). The colony forming efficiency (CFE), the proliferation rate and the frequency of micronucleated cells were measured in these cultures. CFE was significantly reduced (p < 0.05) by β-carotene treatment in cells from healthy mucosa and in KB cells. Decreases (p < 0.05; NS) were also observed in cells from pathological mucosa and in SCC-25 cells. Cell proliferation rate was not substantially affected by β-carotene in all cultures. Finally, a decreased frequency of micronucleated cells was found in treated cultures, but significant reductions (p < 0.05) were only observed in cultures from oral mucosa (healthy and pathological) as well as in KB cell cultures. Our results indicate that β-carotene is able to reduce the clonogenic activity (CFE), even if it does not seem to influence cell proliferation, and that it has a protective effect against genotoxic damage.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>beta Carotene</subject><subject>Biopsy</subject><subject>Carcinoma, Squamous Cell - drug therapy</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Carcinoma, Squamous Cell - ultrastructure</subject><subject>Carotenoids - pharmacology</subject><subject>Cell Division - drug effects</subject><subject>Cell Division - physiology</subject><subject>Cells, Cultured</subject><subject>Female</subject><subject>Humans</subject><subject>Keratinocytes - cytology</subject><subject>Keratinocytes - drug effects</subject><subject>Keratinocytes - ultrastructure</subject><subject>Male</subject><subject>Micronucleus Tests</subject><subject>Middle Aged</subject><subject>Mouth Neoplasms - drug therapy</subject><subject>Mouth Neoplasms - pathology</subject><subject>Mouth Neoplasms - ultrastructure</subject><subject>Precancerous Conditions - drug therapy</subject><subject>Precancerous Conditions - pathology</subject><subject>Precancerous Conditions - ultrastructure</subject><subject>Stem Cells - drug effects</subject><issn>0959-4973</issn><issn>1473-5741</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1UcFu2zAMFYYOWZrtEwbotJtXybIt6zgUbRegwC7tWaBlCvEiS6lkL8hv9UP2TVWWrD2VF4KP75HEIyGUs--cKXnFcvCWi4IrxXmZq-IIqQ9kySspilpW_IIsmapVUSkpPpHLlH5nRsbFgix4yxom6iVxa0__DFMMFK1FMyUaLP37XBiIYUKPNHi6mUfwNERwdIsRpsEHc5gwURvDSHcRDXiDMcyJOkxD8ImC72l6mmE8gnmWyZoRPpOPFlzCL-e8Io-3Nw_XP4v7X3fr6x_3hSklU0UHPValBARrWduzGi2Dpqn6UnRlX3ddr2SPrWJc1JxLZhoJsmyaugUhVAliRb6d5u5ieJoxTXockkHnwGM-SMu8pmmFysT2RDQxpBTR6l0cRogHzZk-Gq3_G61fjf4HHaVfzzvmbsT-TXhyNverU38f3IQxbd28x6g3CG7a6Pf-J14AM0KKqQ</recordid><startdate>199112</startdate><enddate>199112</enddate><creator>Toma, Salvatore</creator><creator>Albanese, Enrico</creator><creator>Palumbo, Raffaella</creator><creator>Nicoló, Guido</creator><creator>Morerio, Cristina</creator><creator>Mangiante, Paolo Erasmo</creator><creator>Bonatti, Stefania</creator><creator>Cancedda, Ranieri</creator><creator>Santi, Leonardo</creator><general>Lippincott-Raven Publishers</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199112</creationdate><title>In vitro effects of β-carotene on human oral keratinocytes from precancerous lesions and squamous carcinoma</title><author>Toma, Salvatore ; Albanese, Enrico ; Palumbo, Raffaella ; Nicoló, Guido ; Morerio, Cristina ; Mangiante, Paolo Erasmo ; Bonatti, Stefania ; Cancedda, Ranieri ; Santi, Leonardo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2709-bade427aeaff08d05ef0a664d23b2d5bbd97de8901351170c67a726658a3392a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>beta Carotene</topic><topic>Biopsy</topic><topic>Carcinoma, Squamous Cell - drug therapy</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>Carcinoma, Squamous Cell - ultrastructure</topic><topic>Carotenoids - pharmacology</topic><topic>Cell Division - drug effects</topic><topic>Cell Division - physiology</topic><topic>Cells, Cultured</topic><topic>Female</topic><topic>Humans</topic><topic>Keratinocytes - cytology</topic><topic>Keratinocytes - drug effects</topic><topic>Keratinocytes - ultrastructure</topic><topic>Male</topic><topic>Micronucleus Tests</topic><topic>Middle Aged</topic><topic>Mouth Neoplasms - drug therapy</topic><topic>Mouth Neoplasms - pathology</topic><topic>Mouth Neoplasms - ultrastructure</topic><topic>Precancerous Conditions - drug therapy</topic><topic>Precancerous Conditions - pathology</topic><topic>Precancerous Conditions - ultrastructure</topic><topic>Stem Cells - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Toma, Salvatore</creatorcontrib><creatorcontrib>Albanese, Enrico</creatorcontrib><creatorcontrib>Palumbo, Raffaella</creatorcontrib><creatorcontrib>Nicoló, Guido</creatorcontrib><creatorcontrib>Morerio, Cristina</creatorcontrib><creatorcontrib>Mangiante, Paolo Erasmo</creatorcontrib><creatorcontrib>Bonatti, Stefania</creatorcontrib><creatorcontrib>Cancedda, Ranieri</creatorcontrib><creatorcontrib>Santi, Leonardo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Anti-cancer drugs</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Toma, Salvatore</au><au>Albanese, Enrico</au><au>Palumbo, Raffaella</au><au>Nicoló, Guido</au><au>Morerio, Cristina</au><au>Mangiante, Paolo Erasmo</au><au>Bonatti, Stefania</au><au>Cancedda, Ranieri</au><au>Santi, Leonardo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro effects of β-carotene on human oral keratinocytes from precancerous lesions and squamous carcinoma</atitle><jtitle>Anti-cancer drugs</jtitle><addtitle>Anticancer Drugs</addtitle><date>1991-12</date><risdate>1991</risdate><volume>2</volume><issue>6</issue><spage>581</spage><epage>590</epage><pages>581-590</pages><issn>0959-4973</issn><eissn>1473-5741</eissn><abstract>Human keratinocytes, obtained from bioptic specimens of healthy and preneoplastic oral mucosa, and from human cell lines from oral cavity tumors (KB and SCC-25) were treated with β-carotene (10 μM). The colony forming efficiency (CFE), the proliferation rate and the frequency of micronucleated cells were measured in these cultures. CFE was significantly reduced (p < 0.05) by β-carotene treatment in cells from healthy mucosa and in KB cells. Decreases (p < 0.05; NS) were also observed in cells from pathological mucosa and in SCC-25 cells. Cell proliferation rate was not substantially affected by β-carotene in all cultures. Finally, a decreased frequency of micronucleated cells was found in treated cultures, but significant reductions (p < 0.05) were only observed in cultures from oral mucosa (healthy and pathological) as well as in KB cell cultures. Our results indicate that β-carotene is able to reduce the clonogenic activity (CFE), even if it does not seem to influence cell proliferation, and that it has a protective effect against genotoxic damage.</abstract><cop>England</cop><pub>Lippincott-Raven Publishers</pub><pmid>1806035</pmid><doi>10.1097/00001813-199112000-00009</doi><tpages>10</tpages></addata></record> |
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| subjects | Adolescent Adult Aged beta Carotene Biopsy Carcinoma, Squamous Cell - drug therapy Carcinoma, Squamous Cell - pathology Carcinoma, Squamous Cell - ultrastructure Carotenoids - pharmacology Cell Division - drug effects Cell Division - physiology Cells, Cultured Female Humans Keratinocytes - cytology Keratinocytes - drug effects Keratinocytes - ultrastructure Male Micronucleus Tests Middle Aged Mouth Neoplasms - drug therapy Mouth Neoplasms - pathology Mouth Neoplasms - ultrastructure Precancerous Conditions - drug therapy Precancerous Conditions - pathology Precancerous Conditions - ultrastructure Stem Cells - drug effects |
| title | In vitro effects of β-carotene on human oral keratinocytes from precancerous lesions and squamous carcinoma |
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