Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine
Pairing of a consensus sequence of the precursor (pre)-mRNA intron with a short region of the U2 small nuclear (sn)RNA during assembly of the eukaryotic spliceosome results in formation of a complementary helix of seven base pairs with a single unpaired adenosine residue. The 2′ OH of this adenosine...
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| Veröffentlicht in: | Nature Structural Biology 2002-12, Vol.9 (12), p.958-965 |
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| creator | Greenbaum, Nancy L Newby, Meredith I |
| description | Pairing of a consensus sequence of the precursor (pre)-mRNA intron with a short region of the U2 small nuclear (sn)RNA during assembly of the eukaryotic spliceosome results in formation of a complementary helix of seven base pairs with a single unpaired adenosine residue. The 2′ OH of this adenosine, called the branch site, brings about nucleophilic attack at the pre-mRNA 5′ splice site in the first step of splicing. Another feature of this pairing is the phylogenetic conservation of a pseudouridine (ψ) residue in U2 snRNA nearly opposite the branch site. We show that the presence of this ψ in the pre-mRNA branch-site helix of
Saccharomyces cerevisiae
induces a dramatically altered architectural landscape compared with that of its unmodified counterpart. The ψ-induced structure places the nucleophile in an accessible position for the first step of splicing. |
| doi_str_mv | 10.1038/nsb873 |
| format | Article |
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Saccharomyces cerevisiae
induces a dramatically altered architectural landscape compared with that of its unmodified counterpart. The ψ-induced structure places the nucleophile in an accessible position for the first step of splicing.</description><identifier>ISSN: 1072-8368</identifier><identifier>ISSN: 1545-9993</identifier><identifier>EISSN: 2331-365X</identifier><identifier>EISSN: 1545-9985</identifier><identifier>DOI: 10.1038/nsb873</identifier><identifier>PMID: 12426583</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>Adenosine - chemistry ; Base Sequence ; Binding Sites ; Biochemistry ; Biological Microscopy ; Biomedical and Life Sciences ; Introns ; Life Sciences ; Membrane Biology ; Models, Molecular ; Nuclear Magnetic Resonance, Biomolecular ; Nucleic Acid Conformation ; Phylogeny ; Protein Structure ; Pseudouridine - chemistry ; Pseudouridine - genetics ; RNA Precursors - chemistry ; RNA Splicing ; RNA, Fungal - chemistry ; Saccharomyces cerevisiae - genetics ; Spliceosomes - chemistry ; Uridine - chemistry</subject><ispartof>Nature Structural Biology, 2002-12, Vol.9 (12), p.958-965</ispartof><rights>Springer Nature America, Inc. 2002</rights><rights>Copyright Nature Publishing Group Dec 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-97745bb157ba24f32af00ab34a82e8cda881c4ff12445c9a35318b858d52c04a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2727,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12426583$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Greenbaum, Nancy L</creatorcontrib><creatorcontrib>Newby, Meredith I</creatorcontrib><title>Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine</title><title>Nature Structural Biology</title><addtitle>Nat Struct Mol Biol</addtitle><addtitle>Nat Struct Biol</addtitle><description>Pairing of a consensus sequence of the precursor (pre)-mRNA intron with a short region of the U2 small nuclear (sn)RNA during assembly of the eukaryotic spliceosome results in formation of a complementary helix of seven base pairs with a single unpaired adenosine residue. The 2′ OH of this adenosine, called the branch site, brings about nucleophilic attack at the pre-mRNA 5′ splice site in the first step of splicing. Another feature of this pairing is the phylogenetic conservation of a pseudouridine (ψ) residue in U2 snRNA nearly opposite the branch site. We show that the presence of this ψ in the pre-mRNA branch-site helix of
Saccharomyces cerevisiae
induces a dramatically altered architectural landscape compared with that of its unmodified counterpart. The ψ-induced structure places the nucleophile in an accessible position for the first step of splicing.</description><subject>Adenosine - chemistry</subject><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>Biochemistry</subject><subject>Biological Microscopy</subject><subject>Biomedical and Life Sciences</subject><subject>Introns</subject><subject>Life Sciences</subject><subject>Membrane Biology</subject><subject>Models, Molecular</subject><subject>Nuclear Magnetic Resonance, Biomolecular</subject><subject>Nucleic Acid Conformation</subject><subject>Phylogeny</subject><subject>Protein Structure</subject><subject>Pseudouridine - chemistry</subject><subject>Pseudouridine - genetics</subject><subject>RNA Precursors - chemistry</subject><subject>RNA Splicing</subject><subject>RNA, Fungal - chemistry</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Spliceosomes - chemistry</subject><subject>Uridine - chemistry</subject><issn>1072-8368</issn><issn>1545-9993</issn><issn>2331-365X</issn><issn>1545-9985</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkctKxDAUhoMoOt6eQCS40FU11zazFPEGggsVZleS9HQm0iY1aYV5eyszOKALV2dxPr5z-RE6puSSEq6ufDKq4FtowjinGc_lbBtNKClYpniu9tB-Su-EUCHIdBftUSZYLhWfoNmLHZqud36OQ437BeDUNc5CSKHVDTZRe7vAyfWAI9gw9653weM29K7GZok1tsEniJ9Q4S7BUIUhusp5OEQ7tW4SHK3rAXq7u329ecienu8fb66fMisk67NpUQhpDJWF0UzUnOmaEG240IqBspVWilpR1-PGQtqp5pJTZZRUlWSWCM0P0PnK28XwMUDqy9YlC02jPYQhlQUrCMkF_RekKi-IENMRPPsFvo9H-fGIkjHF8vGHZGOzMaQUoS676FodlyUl5Xci5SqRETxd2wbTQrXB1hGMwMUKSGPLzyFuxv1RnaxIr_shwo9q3f4C5-OdmA</recordid><startdate>20021201</startdate><enddate>20021201</enddate><creator>Greenbaum, Nancy L</creator><creator>Newby, Meredith I</creator><general>Nature Publishing Group US</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PADUT</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20021201</creationdate><title>Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine</title><author>Greenbaum, Nancy L ; Newby, Meredith I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-97745bb157ba24f32af00ab34a82e8cda881c4ff12445c9a35318b858d52c04a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adenosine - chemistry</topic><topic>Base Sequence</topic><topic>Binding Sites</topic><topic>Biochemistry</topic><topic>Biological Microscopy</topic><topic>Biomedical and Life Sciences</topic><topic>Introns</topic><topic>Life Sciences</topic><topic>Membrane Biology</topic><topic>Models, Molecular</topic><topic>Nuclear Magnetic Resonance, Biomolecular</topic><topic>Nucleic Acid Conformation</topic><topic>Phylogeny</topic><topic>Protein Structure</topic><topic>Pseudouridine - chemistry</topic><topic>Pseudouridine - genetics</topic><topic>RNA Precursors - chemistry</topic><topic>RNA Splicing</topic><topic>RNA, Fungal - chemistry</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Spliceosomes - chemistry</topic><topic>Uridine - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Greenbaum, Nancy L</creatorcontrib><creatorcontrib>Newby, Meredith I</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Research Library China</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Nature Structural Biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Greenbaum, Nancy L</au><au>Newby, Meredith I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine</atitle><jtitle>Nature Structural Biology</jtitle><stitle>Nat Struct Mol Biol</stitle><addtitle>Nat Struct Biol</addtitle><date>2002-12-01</date><risdate>2002</risdate><volume>9</volume><issue>12</issue><spage>958</spage><epage>965</epage><pages>958-965</pages><issn>1072-8368</issn><issn>1545-9993</issn><eissn>2331-365X</eissn><eissn>1545-9985</eissn><abstract>Pairing of a consensus sequence of the precursor (pre)-mRNA intron with a short region of the U2 small nuclear (sn)RNA during assembly of the eukaryotic spliceosome results in formation of a complementary helix of seven base pairs with a single unpaired adenosine residue. The 2′ OH of this adenosine, called the branch site, brings about nucleophilic attack at the pre-mRNA 5′ splice site in the first step of splicing. Another feature of this pairing is the phylogenetic conservation of a pseudouridine (ψ) residue in U2 snRNA nearly opposite the branch site. We show that the presence of this ψ in the pre-mRNA branch-site helix of
Saccharomyces cerevisiae
induces a dramatically altered architectural landscape compared with that of its unmodified counterpart. The ψ-induced structure places the nucleophile in an accessible position for the first step of splicing.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>12426583</pmid><doi>10.1038/nsb873</doi><tpages>8</tpages></addata></record> |
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| subjects | Adenosine - chemistry Base Sequence Binding Sites Biochemistry Biological Microscopy Biomedical and Life Sciences Introns Life Sciences Membrane Biology Models, Molecular Nuclear Magnetic Resonance, Biomolecular Nucleic Acid Conformation Phylogeny Protein Structure Pseudouridine - chemistry Pseudouridine - genetics RNA Precursors - chemistry RNA Splicing RNA, Fungal - chemistry Saccharomyces cerevisiae - genetics Spliceosomes - chemistry Uridine - chemistry |
| title | Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine |
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