Investigation and validation of the affinity chromatography method for measuring glycated albumin in serum and urine
Affinity chromatography on m-aminophenyl boronate columns together with albumin measurement by radioimmunoassay has been validated as a method for determining glycated albumin in serum and urine. Optimisation of sample volume and of elution buffer composition and volume ensured reproducibility of re...
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| Veröffentlicht in: | Clinica chimica acta 1991-10, Vol.202 (1), p.11-22 |
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| creator | Silver, Anne C. Lamb, Edmund Cattell, William R. Dawnay, Anne B.St.J. |
| description | Affinity chromatography on m-aminophenyl boronate columns together with albumin measurement by radioimmunoassay has been validated as a method for determining glycated albumin in serum and urine. Optimisation of sample volume and of elution buffer composition and volume ensured reproducibility of results. Fructosamine assay confirmed the absence of glycated albumin species from the non-glycated fraction. It was possible to elute the glycated fraction from the affinity columns with Tris or glycine which do not contain 1, 2 diols but have similar functional groups. Column affinity was, therefore, not specific for glycated protein moieties. Inhibition of binding by glucose, and other small molecules in urine, necessitated ultrafiltration or dialysis of samples before analysis. Reference ranges for glycated albumin in non-diabetic subjects were 0.6–1.8% in serum and 0.9–2.6% in urine. In patients with diabetes mellitus, glycated albumin ranged from 1.4–10.9% in serum and from 1.5–12.5% in urine. |
| doi_str_mv | 10.1016/0009-8981(91)90251-7 |
| format | Article |
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Optimisation of sample volume and of elution buffer composition and volume ensured reproducibility of results. Fructosamine assay confirmed the absence of glycated albumin species from the non-glycated fraction. It was possible to elute the glycated fraction from the affinity columns with Tris or glycine which do not contain 1, 2 diols but have similar functional groups. Column affinity was, therefore, not specific for glycated protein moieties. Inhibition of binding by glucose, and other small molecules in urine, necessitated ultrafiltration or dialysis of samples before analysis. Reference ranges for glycated albumin in non-diabetic subjects were 0.6–1.8% in serum and 0.9–2.6% in urine. In patients with diabetes mellitus, glycated albumin ranged from 1.4–10.9% in serum and from 1.5–12.5% in urine.</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/0009-8981(91)90251-7</identifier><identifier>PMID: 1807865</identifier><identifier>CODEN: CCATAR</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Adult ; Albuminuria ; Analytical, structural and metabolic biochemistry ; Binding and carrier proteins ; Biological and medical sciences ; Biomarkers - blood ; Biomarkers - urine ; Chromatography, Affinity - methods ; Diabetes Mellitus - blood ; Diabetes Mellitus - urine ; Fructosamine ; Fundamental and applied biological sciences. Psychology ; Glycated albumin ; Glycated protein ; Glycosuria ; Glycosylation ; Hexosamines - blood ; Humans ; m-Aminophenyl boronate affinity chromatography ; Middle Aged ; Proteins ; Radioimmunoassay - methods ; Reference Values ; Serum Albumin - analysis ; Urine protein</subject><ispartof>Clinica chimica acta, 1991-10, Vol.202 (1), p.11-22</ispartof><rights>1991</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-143b40a74f2d429d4aa936a2c22e847b048f54037801a36c821417a0f44716af3</citedby><cites>FETCH-LOGICAL-c452t-143b40a74f2d429d4aa936a2c22e847b048f54037801a36c821417a0f44716af3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0009-8981(91)90251-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5044740$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1807865$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Silver, Anne C.</creatorcontrib><creatorcontrib>Lamb, Edmund</creatorcontrib><creatorcontrib>Cattell, William R.</creatorcontrib><creatorcontrib>Dawnay, Anne B.St.J.</creatorcontrib><title>Investigation and validation of the affinity chromatography method for measuring glycated albumin in serum and urine</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>Affinity chromatography on m-aminophenyl boronate columns together with albumin measurement by radioimmunoassay has been validated as a method for determining glycated albumin in serum and urine. Optimisation of sample volume and of elution buffer composition and volume ensured reproducibility of results. Fructosamine assay confirmed the absence of glycated albumin species from the non-glycated fraction. It was possible to elute the glycated fraction from the affinity columns with Tris or glycine which do not contain 1, 2 diols but have similar functional groups. Column affinity was, therefore, not specific for glycated protein moieties. Inhibition of binding by glucose, and other small molecules in urine, necessitated ultrafiltration or dialysis of samples before analysis. Reference ranges for glycated albumin in non-diabetic subjects were 0.6–1.8% in serum and 0.9–2.6% in urine. In patients with diabetes mellitus, glycated albumin ranged from 1.4–10.9% in serum and from 1.5–12.5% in urine.</description><subject>Adult</subject><subject>Albuminuria</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Binding and carrier proteins</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - blood</subject><subject>Biomarkers - urine</subject><subject>Chromatography, Affinity - methods</subject><subject>Diabetes Mellitus - blood</subject><subject>Diabetes Mellitus - urine</subject><subject>Fructosamine</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycated albumin</subject><subject>Glycated protein</subject><subject>Glycosuria</subject><subject>Glycosylation</subject><subject>Hexosamines - blood</subject><subject>Humans</subject><subject>m-Aminophenyl boronate affinity chromatography</subject><subject>Middle Aged</subject><subject>Proteins</subject><subject>Radioimmunoassay - methods</subject><subject>Reference Values</subject><subject>Serum Albumin - analysis</subject><subject>Urine protein</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kN9rFDEQgIMo9Wz9DxTyIEUf1ibZ7GbzIkhpa6Hgiz6Hufy4i-wmZ5I9uP_erHvUN2EgGeabyeRD6B0lnymh_Q0hRDaDHOhHST9JwjraiBdoQwfRNi2X7CXaPCOv0Zucf9WUk55eoAs6EDH03QaVx3C0ufgdFB8DhmDwEUZv1jQ6XPYWg3M--HLCep_iBCXuEhz2JzzZso8Gu5jqFfKcfNjh3XjSUKzBMG7nyQdcI9s0T3-HL4y9Qq8cjNm-PZ-X6Of93Y_bb83T94fH269PjeYdKw3l7ZYTENwxw5k0HEC2PTDNmB242BI-uI6TVgyEQtvrgVFOBRDHuaA9uPYSXa9zDyn-nus31eSztuMIwcY5K8F6KXlLKshXUKeYc7JOHZKfIJ0UJWqRrRaTajGpZI1FthK17f15_rydrPnXtNqt9Q_nOmQNo0sQtM_PWEfqonx5_cuK2eri6G1SWXsbtDU-WV2Uif7_e_wBFHmbsw</recordid><startdate>19911014</startdate><enddate>19911014</enddate><creator>Silver, Anne C.</creator><creator>Lamb, Edmund</creator><creator>Cattell, William R.</creator><creator>Dawnay, Anne B.St.J.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19911014</creationdate><title>Investigation and validation of the affinity chromatography method for measuring glycated albumin in serum and urine</title><author>Silver, Anne C. ; Lamb, Edmund ; Cattell, William R. ; Dawnay, Anne B.St.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-143b40a74f2d429d4aa936a2c22e847b048f54037801a36c821417a0f44716af3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Adult</topic><topic>Albuminuria</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Binding and carrier proteins</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - blood</topic><topic>Biomarkers - urine</topic><topic>Chromatography, Affinity - methods</topic><topic>Diabetes Mellitus - blood</topic><topic>Diabetes Mellitus - urine</topic><topic>Fructosamine</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycated albumin</topic><topic>Glycated protein</topic><topic>Glycosuria</topic><topic>Glycosylation</topic><topic>Hexosamines - blood</topic><topic>Humans</topic><topic>m-Aminophenyl boronate affinity chromatography</topic><topic>Middle Aged</topic><topic>Proteins</topic><topic>Radioimmunoassay - methods</topic><topic>Reference Values</topic><topic>Serum Albumin - analysis</topic><topic>Urine protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Silver, Anne C.</creatorcontrib><creatorcontrib>Lamb, Edmund</creatorcontrib><creatorcontrib>Cattell, William R.</creatorcontrib><creatorcontrib>Dawnay, Anne B.St.J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Silver, Anne C.</au><au>Lamb, Edmund</au><au>Cattell, William R.</au><au>Dawnay, Anne B.St.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigation and validation of the affinity chromatography method for measuring glycated albumin in serum and urine</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>1991-10-14</date><risdate>1991</risdate><volume>202</volume><issue>1</issue><spage>11</spage><epage>22</epage><pages>11-22</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><coden>CCATAR</coden><abstract>Affinity chromatography on m-aminophenyl boronate columns together with albumin measurement by radioimmunoassay has been validated as a method for determining glycated albumin in serum and urine. Optimisation of sample volume and of elution buffer composition and volume ensured reproducibility of results. Fructosamine assay confirmed the absence of glycated albumin species from the non-glycated fraction. It was possible to elute the glycated fraction from the affinity columns with Tris or glycine which do not contain 1, 2 diols but have similar functional groups. Column affinity was, therefore, not specific for glycated protein moieties. Inhibition of binding by glucose, and other small molecules in urine, necessitated ultrafiltration or dialysis of samples before analysis. Reference ranges for glycated albumin in non-diabetic subjects were 0.6–1.8% in serum and 0.9–2.6% in urine. In patients with diabetes mellitus, glycated albumin ranged from 1.4–10.9% in serum and from 1.5–12.5% in urine.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>1807865</pmid><doi>10.1016/0009-8981(91)90251-7</doi><tpages>12</tpages></addata></record> |
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| subjects | Adult Albuminuria Analytical, structural and metabolic biochemistry Binding and carrier proteins Biological and medical sciences Biomarkers - blood Biomarkers - urine Chromatography, Affinity - methods Diabetes Mellitus - blood Diabetes Mellitus - urine Fructosamine Fundamental and applied biological sciences. Psychology Glycated albumin Glycated protein Glycosuria Glycosylation Hexosamines - blood Humans m-Aminophenyl boronate affinity chromatography Middle Aged Proteins Radioimmunoassay - methods Reference Values Serum Albumin - analysis Urine protein |
| title | Investigation and validation of the affinity chromatography method for measuring glycated albumin in serum and urine |
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