Characterization of [ 3H]U69593 binding sites in the rat heart by receptor binding assays
The binding properties of [ 3H]U69593, a selective k-ligand, in rat heart homogenates were characterized by direct and displacement receptor binding assays. It was found that there are substantial specific [ 3H]UU69593 binding sites in the rat heart. They were saturable, reversible and stereospecifi...
Gespeichert in:
| Veröffentlicht in: | Journal of molecular and cellular cardiology 1991-11, Vol.23 (11), p.1297-1302 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1302 |
|---|---|
| container_issue | 11 |
| container_start_page | 1297 |
| container_title | Journal of molecular and cellular cardiology |
| container_volume | 23 |
| creator | Tai, K.K. Jin, W-Q. Chan, T.K.Y. Wong, T.M. |
| description | The binding properties of [
3H]U69593, a selective k-ligand, in rat heart homogenates were characterized by direct and displacement receptor binding assays. It was found that there are substantial specific [
3H]UU69593 binding sites in the rat heart. They were saturable, reversible and stereospecific. Both association and dissociation rates were monophasic and the Scatchard plot was linear, indicating a homogeneity of binding sites. The Hill coefficient was close ti 1, indicating an absence of cooperativity. The
B
max and
K
d were 7.91 fmol/mg and 2.92 n
m, respectively. The binding sites were most abundant in the right atrium, followed by right ventricle, left ventricle and left atrium in descending order. The study provides information on the properties and distribution of k-binding sites in the rat heart. |
| doi_str_mv | 10.1016/0022-2828(91)90086-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72699104</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0022282891900862</els_id><sourcerecordid>72699104</sourcerecordid><originalsourceid>FETCH-LOGICAL-c386t-cff7a05266a681366027d525d2eebee9f3ccce3378e87eebdd29fbbb13d78f713</originalsourceid><addsrcrecordid>eNp9kMFqGzEQhkVoSZ00b5CCDqWkh01HkqWVLoFi2iYQ6CU5lFKEVhrFCvauI8kF5-mzro17y2lg5vt_ho-QcwaXDJj6AsB5wzXXF4Z9NgBaNfyITBgY2Wipp2_I5IC8IyelPAKAmQpxTI6ZBgEcJuTXbO6y8xVzenY1DT0dIv1NxfWfe2WkEbRLfUj9Ay2pYqGpp3WONLtK5-hypd2GZvS4qkM-oK4UtynvydvoFgXP9vOU3H__dje7bm5__riZfb1tvNCqNj7G1oHkSjmlmVAKeBskl4EjdogmCu89CtFq1O24CoGb2HUdE6HVsWXilHza9a7y8LTGUu0yFY-LhetxWBfbcmUMg-kITnegz0MpGaNd5bR0eWMZ2K1Ru9Vlt7qsYfafUcvH2Id9_7pbYvgf2ikc7x_3d1e8W8Tsep_KAZNMGgNyxK52GI4u_ibMtviEvceQRoHVhiG9_scLhHqROw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72699104</pqid></control><display><type>article</type><title>Characterization of [ 3H]U69593 binding sites in the rat heart by receptor binding assays</title><source>Elsevier ScienceDirect Journals Complete - AutoHoldings</source><source>MEDLINE</source><creator>Tai, K.K. ; Jin, W-Q. ; Chan, T.K.Y. ; Wong, T.M.</creator><creatorcontrib>Tai, K.K. ; Jin, W-Q. ; Chan, T.K.Y. ; Wong, T.M.</creatorcontrib><description>The binding properties of [
3H]U69593, a selective k-ligand, in rat heart homogenates were characterized by direct and displacement receptor binding assays. It was found that there are substantial specific [
3H]UU69593 binding sites in the rat heart. They were saturable, reversible and stereospecific. Both association and dissociation rates were monophasic and the Scatchard plot was linear, indicating a homogeneity of binding sites. The Hill coefficient was close ti 1, indicating an absence of cooperativity. The
B
max and
K
d were 7.91 fmol/mg and 2.92 n
m, respectively. The binding sites were most abundant in the right atrium, followed by right ventricle, left ventricle and left atrium in descending order. The study provides information on the properties and distribution of k-binding sites in the rat heart.</description><identifier>ISSN: 0022-2828</identifier><identifier>EISSN: 1095-8584</identifier><identifier>DOI: 10.1016/0022-2828(91)90086-2</identifier><identifier>PMID: 1803020</identifier><identifier>CODEN: JMCDAY</identifier><language>eng</language><publisher>Kent: Elsevier Ltd</publisher><subject>Analgesics - metabolism ; Animals ; Benzeneacetamides ; Binding Sites ; Biological and medical sciences ; Cell receptors ; Cell structures and functions ; Female ; Fundamental and applied biological sciences. Psychology ; k-binding sites ; Kinetics ; Molecular and cellular biology ; Myocardium - metabolism ; Neuropeptide receptors ; Pyrrolidines - metabolism ; Radioligand Assay ; Rat heart ; Rats ; Rats, Inbred Strains ; Receptor binding ; U-69593 ; U50,448H</subject><ispartof>Journal of molecular and cellular cardiology, 1991-11, Vol.23 (11), p.1297-1302</ispartof><rights>1991</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-cff7a05266a681366027d525d2eebee9f3ccce3378e87eebdd29fbbb13d78f713</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0022-2828(91)90086-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5159905$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1803020$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tai, K.K.</creatorcontrib><creatorcontrib>Jin, W-Q.</creatorcontrib><creatorcontrib>Chan, T.K.Y.</creatorcontrib><creatorcontrib>Wong, T.M.</creatorcontrib><title>Characterization of [ 3H]U69593 binding sites in the rat heart by receptor binding assays</title><title>Journal of molecular and cellular cardiology</title><addtitle>J Mol Cell Cardiol</addtitle><description>The binding properties of [
3H]U69593, a selective k-ligand, in rat heart homogenates were characterized by direct and displacement receptor binding assays. It was found that there are substantial specific [
3H]UU69593 binding sites in the rat heart. They were saturable, reversible and stereospecific. Both association and dissociation rates were monophasic and the Scatchard plot was linear, indicating a homogeneity of binding sites. The Hill coefficient was close ti 1, indicating an absence of cooperativity. The
B
max and
K
d were 7.91 fmol/mg and 2.92 n
m, respectively. The binding sites were most abundant in the right atrium, followed by right ventricle, left ventricle and left atrium in descending order. The study provides information on the properties and distribution of k-binding sites in the rat heart.</description><subject>Analgesics - metabolism</subject><subject>Animals</subject><subject>Benzeneacetamides</subject><subject>Binding Sites</subject><subject>Biological and medical sciences</subject><subject>Cell receptors</subject><subject>Cell structures and functions</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>k-binding sites</subject><subject>Kinetics</subject><subject>Molecular and cellular biology</subject><subject>Myocardium - metabolism</subject><subject>Neuropeptide receptors</subject><subject>Pyrrolidines - metabolism</subject><subject>Radioligand Assay</subject><subject>Rat heart</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Receptor binding</subject><subject>U-69593</subject><subject>U50,448H</subject><issn>0022-2828</issn><issn>1095-8584</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFqGzEQhkVoSZ00b5CCDqWkh01HkqWVLoFi2iYQ6CU5lFKEVhrFCvauI8kF5-mzro17y2lg5vt_ho-QcwaXDJj6AsB5wzXXF4Z9NgBaNfyITBgY2Wipp2_I5IC8IyelPAKAmQpxTI6ZBgEcJuTXbO6y8xVzenY1DT0dIv1NxfWfe2WkEbRLfUj9Ay2pYqGpp3WONLtK5-hypd2GZvS4qkM-oK4UtynvydvoFgXP9vOU3H__dje7bm5__riZfb1tvNCqNj7G1oHkSjmlmVAKeBskl4EjdogmCu89CtFq1O24CoGb2HUdE6HVsWXilHza9a7y8LTGUu0yFY-LhetxWBfbcmUMg-kITnegz0MpGaNd5bR0eWMZ2K1Ru9Vlt7qsYfafUcvH2Id9_7pbYvgf2ikc7x_3d1e8W8Tsep_KAZNMGgNyxK52GI4u_ibMtviEvceQRoHVhiG9_scLhHqROw</recordid><startdate>19911101</startdate><enddate>19911101</enddate><creator>Tai, K.K.</creator><creator>Jin, W-Q.</creator><creator>Chan, T.K.Y.</creator><creator>Wong, T.M.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19911101</creationdate><title>Characterization of [ 3H]U69593 binding sites in the rat heart by receptor binding assays</title><author>Tai, K.K. ; Jin, W-Q. ; Chan, T.K.Y. ; Wong, T.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-cff7a05266a681366027d525d2eebee9f3ccce3378e87eebdd29fbbb13d78f713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Analgesics - metabolism</topic><topic>Animals</topic><topic>Benzeneacetamides</topic><topic>Binding Sites</topic><topic>Biological and medical sciences</topic><topic>Cell receptors</topic><topic>Cell structures and functions</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>k-binding sites</topic><topic>Kinetics</topic><topic>Molecular and cellular biology</topic><topic>Myocardium - metabolism</topic><topic>Neuropeptide receptors</topic><topic>Pyrrolidines - metabolism</topic><topic>Radioligand Assay</topic><topic>Rat heart</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Receptor binding</topic><topic>U-69593</topic><topic>U50,448H</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tai, K.K.</creatorcontrib><creatorcontrib>Jin, W-Q.</creatorcontrib><creatorcontrib>Chan, T.K.Y.</creatorcontrib><creatorcontrib>Wong, T.M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular and cellular cardiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tai, K.K.</au><au>Jin, W-Q.</au><au>Chan, T.K.Y.</au><au>Wong, T.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of [ 3H]U69593 binding sites in the rat heart by receptor binding assays</atitle><jtitle>Journal of molecular and cellular cardiology</jtitle><addtitle>J Mol Cell Cardiol</addtitle><date>1991-11-01</date><risdate>1991</risdate><volume>23</volume><issue>11</issue><spage>1297</spage><epage>1302</epage><pages>1297-1302</pages><issn>0022-2828</issn><eissn>1095-8584</eissn><coden>JMCDAY</coden><abstract>The binding properties of [
3H]U69593, a selective k-ligand, in rat heart homogenates were characterized by direct and displacement receptor binding assays. It was found that there are substantial specific [
3H]UU69593 binding sites in the rat heart. They were saturable, reversible and stereospecific. Both association and dissociation rates were monophasic and the Scatchard plot was linear, indicating a homogeneity of binding sites. The Hill coefficient was close ti 1, indicating an absence of cooperativity. The
B
max and
K
d were 7.91 fmol/mg and 2.92 n
m, respectively. The binding sites were most abundant in the right atrium, followed by right ventricle, left ventricle and left atrium in descending order. The study provides information on the properties and distribution of k-binding sites in the rat heart.</abstract><cop>Kent</cop><pub>Elsevier Ltd</pub><pmid>1803020</pmid><doi>10.1016/0022-2828(91)90086-2</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-2828 |
| ispartof | Journal of molecular and cellular cardiology, 1991-11, Vol.23 (11), p.1297-1302 |
| issn | 0022-2828 1095-8584 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72699104 |
| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Analgesics - metabolism Animals Benzeneacetamides Binding Sites Biological and medical sciences Cell receptors Cell structures and functions Female Fundamental and applied biological sciences. Psychology k-binding sites Kinetics Molecular and cellular biology Myocardium - metabolism Neuropeptide receptors Pyrrolidines - metabolism Radioligand Assay Rat heart Rats Rats, Inbred Strains Receptor binding U-69593 U50,448H |
| title | Characterization of [ 3H]U69593 binding sites in the rat heart by receptor binding assays |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T01%3A30%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20of%20%5B%203H%5DU69593%20binding%20sites%20in%20the%20rat%20heart%20by%20receptor%20binding%20assays&rft.jtitle=Journal%20of%20molecular%20and%20cellular%20cardiology&rft.au=Tai,%20K.K.&rft.date=1991-11-01&rft.volume=23&rft.issue=11&rft.spage=1297&rft.epage=1302&rft.pages=1297-1302&rft.issn=0022-2828&rft.eissn=1095-8584&rft.coden=JMCDAY&rft_id=info:doi/10.1016/0022-2828(91)90086-2&rft_dat=%3Cproquest_cross%3E72699104%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=72699104&rft_id=info:pmid/1803020&rft_els_id=0022282891900862&rfr_iscdi=true |