Asialoglycoprotein receptor in human isolated hepatocytes from normal liver and its apparent increase in liver with histological alterations
We have characterized a binding site for galactosyl terminal glycoproteins in hepatocytes isolated from human biopsies. The binding of asialoorosomucoid on hepatocytes previously treated by Triton X-100 was saturable, calcium-dependent and highly affine ( K a = 1.11 ± 0.87 · 10 9 M −1) thus correspo...
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| Veröffentlicht in: | Journal of hepatology 1991-11, Vol.13 (3), p.305-309 |
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| container_title | Journal of hepatology |
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| creator | Eisenberg, Claude Seta, Nathalie Appel, Martine Feldmann, Gerard Durand, Genevieve Feger, Jeanne |
| description | We have characterized a binding site for galactosyl terminal glycoproteins in hepatocytes isolated from human biopsies. The binding of asialoorosomucoid on hepatocytes previously treated by Triton X-100 was saturable, calcium-dependent and highly affine (
K
a = 1.11 ± 0.87 · 10
9 M
−1) thus corresponding to a ligand-receptor binding. The total number of receptors in the normal human liver was 140 000 ± 65 000 sites per cell. This corresponded to the value obtained in the human hepatoma cell line HepG2, but was significantly lower than for isolated rat hepatocytes. Furthermore, in hepatocytes isolated from livers with histological features of either fibrosis, cirrhosis, hepatocarcinoma with cirrhosis or nodular regenerative hyperplasia, the number of asialoglycoprotein receptors per cell was increased, while the binding affinity was unchanged. |
| doi_str_mv | 10.1016/0168-8278(91)90073-K |
| format | Article |
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K
a = 1.11 ± 0.87 · 10
9 M
−1) thus corresponding to a ligand-receptor binding. The total number of receptors in the normal human liver was 140 000 ± 65 000 sites per cell. This corresponded to the value obtained in the human hepatoma cell line HepG2, but was significantly lower than for isolated rat hepatocytes. Furthermore, in hepatocytes isolated from livers with histological features of either fibrosis, cirrhosis, hepatocarcinoma with cirrhosis or nodular regenerative hyperplasia, the number of asialoglycoprotein receptors per cell was increased, while the binding affinity was unchanged.</description><identifier>ISSN: 0168-8278</identifier><identifier>EISSN: 1600-0641</identifier><identifier>DOI: 10.1016/0168-8278(91)90073-K</identifier><identifier>PMID: 1808223</identifier><identifier>CODEN: JOHEEC</identifier><language>eng</language><publisher>Oxford: Elsevier B.V</publisher><subject>Adult ; Aged ; Asialoglycoprotein Receptor ; Asialoglycoproteins - metabolism ; Biological and medical sciences ; Biopsy ; Cells, Cultured ; Female ; Gallbladder Diseases - immunology ; Gallbladder Diseases - metabolism ; Gallbladder Diseases - pathology ; Gastroenterology. Liver. Pancreas. Abdomen ; Humans ; Kinetics ; Liver - immunology ; Liver - metabolism ; Liver - pathology ; Liver Diseases - immunology ; Liver Diseases - metabolism ; Liver Diseases - pathology ; Male ; Medical sciences ; Middle Aged ; Orosomucoid - analogs & derivatives ; Orosomucoid - metabolism ; Receptors, Immunologic - analysis ; Receptors, Immunologic - metabolism ; Reference Values</subject><ispartof>Journal of hepatology, 1991-11, Vol.13 (3), p.305-309</ispartof><rights>1991</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-176148fce625b018e2fb1dbc75545978482ffbb956ebfa74f22ce05df65ab9fe3</citedby><cites>FETCH-LOGICAL-c386t-176148fce625b018e2fb1dbc75545978482ffbb956ebfa74f22ce05df65ab9fe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0168-8278(91)90073-K$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5129709$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1808223$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Eisenberg, Claude</creatorcontrib><creatorcontrib>Seta, Nathalie</creatorcontrib><creatorcontrib>Appel, Martine</creatorcontrib><creatorcontrib>Feldmann, Gerard</creatorcontrib><creatorcontrib>Durand, Genevieve</creatorcontrib><creatorcontrib>Feger, Jeanne</creatorcontrib><title>Asialoglycoprotein receptor in human isolated hepatocytes from normal liver and its apparent increase in liver with histological alterations</title><title>Journal of hepatology</title><addtitle>J Hepatol</addtitle><description>We have characterized a binding site for galactosyl terminal glycoproteins in hepatocytes isolated from human biopsies. The binding of asialoorosomucoid on hepatocytes previously treated by Triton X-100 was saturable, calcium-dependent and highly affine (
K
a = 1.11 ± 0.87 · 10
9 M
−1) thus corresponding to a ligand-receptor binding. The total number of receptors in the normal human liver was 140 000 ± 65 000 sites per cell. This corresponded to the value obtained in the human hepatoma cell line HepG2, but was significantly lower than for isolated rat hepatocytes. Furthermore, in hepatocytes isolated from livers with histological features of either fibrosis, cirrhosis, hepatocarcinoma with cirrhosis or nodular regenerative hyperplasia, the number of asialoglycoprotein receptors per cell was increased, while the binding affinity was unchanged.</description><subject>Adult</subject><subject>Aged</subject><subject>Asialoglycoprotein Receptor</subject><subject>Asialoglycoproteins - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biopsy</subject><subject>Cells, Cultured</subject><subject>Female</subject><subject>Gallbladder Diseases - immunology</subject><subject>Gallbladder Diseases - metabolism</subject><subject>Gallbladder Diseases - pathology</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Humans</subject><subject>Kinetics</subject><subject>Liver - immunology</subject><subject>Liver - metabolism</subject><subject>Liver - pathology</subject><subject>Liver Diseases - immunology</subject><subject>Liver Diseases - metabolism</subject><subject>Liver Diseases - pathology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Orosomucoid - analogs & derivatives</subject><subject>Orosomucoid - metabolism</subject><subject>Receptors, Immunologic - analysis</subject><subject>Receptors, Immunologic - metabolism</subject><subject>Reference Values</subject><issn>0168-8278</issn><issn>1600-0641</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc9uFSEYxYmxqbfVN9CEhTF1MRWYGQY2TZqm_kmbuNE1YZgPL4aBEbg19x360HKdm7pzQSD5fucA5yD0mpJLSij_UJdoBBvEhaTvJSFD29w9QxvKCWkI7-hztHlCXqCznH8SQloiu1N0SgURjLUb9Hidnfbxh9-buKRYwAWcwMBSYsL1vN3NOmCXo9cFJryFRZdo9gUytinOOMQ0a4-9e4CEdZiwKxnrZdEJQqkGJoHOcHBakd-ubPHW5RLrpc5UqfYFki4uhvwSnVjtM7w67ufo-8fbbzefm_uvn77cXN83phW8NHTgtBPWAGf9SKgAZkc6jWbo-66Xg-gEs3YcZc9htHroLGMGSD9Z3utRWmjP0bvVt_741w5yUbPLBrzXAeIuq4FxKQTpKtitoEkx5wRWLcnNOu0VJepQgjokrA4JK0nV3xLUXZW9Ofrvxhmmf6I19Tp_e5zrXCOwSQfj8hPWUyYHIit2tWJQs3hwkFQ2DoKBydWKipqi-_87_gD-GKei</recordid><startdate>19911101</startdate><enddate>19911101</enddate><creator>Eisenberg, Claude</creator><creator>Seta, Nathalie</creator><creator>Appel, Martine</creator><creator>Feldmann, Gerard</creator><creator>Durand, Genevieve</creator><creator>Feger, Jeanne</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19911101</creationdate><title>Asialoglycoprotein receptor in human isolated hepatocytes from normal liver and its apparent increase in liver with histological alterations</title><author>Eisenberg, Claude ; Seta, Nathalie ; Appel, Martine ; Feldmann, Gerard ; Durand, Genevieve ; Feger, Jeanne</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-176148fce625b018e2fb1dbc75545978482ffbb956ebfa74f22ce05df65ab9fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Asialoglycoprotein Receptor</topic><topic>Asialoglycoproteins - metabolism</topic><topic>Biological and medical sciences</topic><topic>Biopsy</topic><topic>Cells, Cultured</topic><topic>Female</topic><topic>Gallbladder Diseases - immunology</topic><topic>Gallbladder Diseases - metabolism</topic><topic>Gallbladder Diseases - pathology</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Humans</topic><topic>Kinetics</topic><topic>Liver - immunology</topic><topic>Liver - metabolism</topic><topic>Liver - pathology</topic><topic>Liver Diseases - immunology</topic><topic>Liver Diseases - metabolism</topic><topic>Liver Diseases - pathology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Orosomucoid - analogs & derivatives</topic><topic>Orosomucoid - metabolism</topic><topic>Receptors, Immunologic - analysis</topic><topic>Receptors, Immunologic - metabolism</topic><topic>Reference Values</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eisenberg, Claude</creatorcontrib><creatorcontrib>Seta, Nathalie</creatorcontrib><creatorcontrib>Appel, Martine</creatorcontrib><creatorcontrib>Feldmann, Gerard</creatorcontrib><creatorcontrib>Durand, Genevieve</creatorcontrib><creatorcontrib>Feger, Jeanne</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of hepatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eisenberg, Claude</au><au>Seta, Nathalie</au><au>Appel, Martine</au><au>Feldmann, Gerard</au><au>Durand, Genevieve</au><au>Feger, Jeanne</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Asialoglycoprotein receptor in human isolated hepatocytes from normal liver and its apparent increase in liver with histological alterations</atitle><jtitle>Journal of hepatology</jtitle><addtitle>J Hepatol</addtitle><date>1991-11-01</date><risdate>1991</risdate><volume>13</volume><issue>3</issue><spage>305</spage><epage>309</epage><pages>305-309</pages><issn>0168-8278</issn><eissn>1600-0641</eissn><coden>JOHEEC</coden><abstract>We have characterized a binding site for galactosyl terminal glycoproteins in hepatocytes isolated from human biopsies. The binding of asialoorosomucoid on hepatocytes previously treated by Triton X-100 was saturable, calcium-dependent and highly affine (
K
a = 1.11 ± 0.87 · 10
9 M
−1) thus corresponding to a ligand-receptor binding. The total number of receptors in the normal human liver was 140 000 ± 65 000 sites per cell. This corresponded to the value obtained in the human hepatoma cell line HepG2, but was significantly lower than for isolated rat hepatocytes. Furthermore, in hepatocytes isolated from livers with histological features of either fibrosis, cirrhosis, hepatocarcinoma with cirrhosis or nodular regenerative hyperplasia, the number of asialoglycoprotein receptors per cell was increased, while the binding affinity was unchanged.</abstract><cop>Oxford</cop><pub>Elsevier B.V</pub><pmid>1808223</pmid><doi>10.1016/0168-8278(91)90073-K</doi><tpages>5</tpages></addata></record> |
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| ispartof | Journal of hepatology, 1991-11, Vol.13 (3), p.305-309 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Adult Aged Asialoglycoprotein Receptor Asialoglycoproteins - metabolism Biological and medical sciences Biopsy Cells, Cultured Female Gallbladder Diseases - immunology Gallbladder Diseases - metabolism Gallbladder Diseases - pathology Gastroenterology. Liver. Pancreas. Abdomen Humans Kinetics Liver - immunology Liver - metabolism Liver - pathology Liver Diseases - immunology Liver Diseases - metabolism Liver Diseases - pathology Male Medical sciences Middle Aged Orosomucoid - analogs & derivatives Orosomucoid - metabolism Receptors, Immunologic - analysis Receptors, Immunologic - metabolism Reference Values |
| title | Asialoglycoprotein receptor in human isolated hepatocytes from normal liver and its apparent increase in liver with histological alterations |
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