Direct analysis of laser capture microdissected cells by MALDI mass spectrometry
Laser capture microdissection (LCM) has become an important tool in biological research, permitting isolation of specific cell populations from frozen tissue samples containing a mixture of cell types. Cells obtained by LCM can be directly analyzed by matrix-assisted laser desorption/ionization mass...
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| Veröffentlicht in: | Journal of the American Society for Mass Spectrometry 2002-11, Vol.13 (11), p.1292-1297 |
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| container_title | Journal of the American Society for Mass Spectrometry |
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| creator | Xu, Baogang J Caprioli, Richard M Sanders, Melinda E Jensen, Roy A |
| description | Laser capture microdissection (LCM) has become an important tool in biological research, permitting isolation of specific cell populations from frozen tissue samples containing a mixture of cell types. Cells obtained by LCM can be directly analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). We report here methodology for the preparation and analysis of LCM captured cells with MALDI MS, giving high sensitivity and mass resolution. Comparison of the spectra obtained from cell populations of interest can identify unique disease or function-related protein markers. Using this approach, mass spectra obtained from human breast tissue containing invasive mammary carcinoma and normal breast epithelium using LCM were compared. Over 40 peaks were identified that significantly differed in intensity between invasive mammary carcinoma and normal breast epithelium. In addition, mass spectra are presented that show protein patterns from mouse liver and mouse colon crypts. The reported tissue preparation procedure and subsequent analysis by MALDI MS provide a new methodology for protein discovery involving LCM captured cells. |
| doi_str_mv | 10.1016/S1044-0305(02)00644-X |
| format | Article |
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Cells obtained by LCM can be directly analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). We report here methodology for the preparation and analysis of LCM captured cells with MALDI MS, giving high sensitivity and mass resolution. Comparison of the spectra obtained from cell populations of interest can identify unique disease or function-related protein markers. Using this approach, mass spectra obtained from human breast tissue containing invasive mammary carcinoma and normal breast epithelium using LCM were compared. Over 40 peaks were identified that significantly differed in intensity between invasive mammary carcinoma and normal breast epithelium. In addition, mass spectra are presented that show protein patterns from mouse liver and mouse colon crypts. The reported tissue preparation procedure and subsequent analysis by MALDI MS provide a new methodology for protein discovery involving LCM captured cells.</description><identifier>ISSN: 1044-0305</identifier><identifier>EISSN: 1879-1123</identifier><identifier>DOI: 10.1016/S1044-0305(02)00644-X</identifier><identifier>PMID: 12443019</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Analytical biochemistry: general aspects, technics, instrumentation ; Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Breast ; Breast - chemistry ; Breast - cytology ; Breast Neoplasms - chemistry ; Breast Neoplasms - pathology ; Cells - chemistry ; Colon ; Colon - chemistry ; Colon - cytology ; Colon - pathology ; Crypts ; Desorption ; Epithelium ; Female ; Fundamental and applied biological sciences. Psychology ; Humans ; Investigative techniques, diagnostic techniques (general aspects) ; Ionization ; Ions ; Lasers ; Liver ; Liver - chemistry ; Liver - cytology ; Liver - pathology ; Mass spectra ; Mass spectrometry ; Mass spectroscopy ; Medical sciences ; Mice ; Miscellaneous. Technology ; Nanotechnology ; Pathology. Cytology. Biochemistry. Spectrometry. 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Cells obtained by LCM can be directly analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). We report here methodology for the preparation and analysis of LCM captured cells with MALDI MS, giving high sensitivity and mass resolution. Comparison of the spectra obtained from cell populations of interest can identify unique disease or function-related protein markers. Using this approach, mass spectra obtained from human breast tissue containing invasive mammary carcinoma and normal breast epithelium using LCM were compared. Over 40 peaks were identified that significantly differed in intensity between invasive mammary carcinoma and normal breast epithelium. In addition, mass spectra are presented that show protein patterns from mouse liver and mouse colon crypts. The reported tissue preparation procedure and subsequent analysis by MALDI MS provide a new methodology for protein discovery involving LCM captured cells.</description><subject>Analytical biochemistry: general aspects, technics, instrumentation</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Breast</subject><subject>Breast - chemistry</subject><subject>Breast - cytology</subject><subject>Breast Neoplasms - chemistry</subject><subject>Breast Neoplasms - pathology</subject><subject>Cells - chemistry</subject><subject>Colon</subject><subject>Colon - chemistry</subject><subject>Colon - cytology</subject><subject>Colon - pathology</subject><subject>Crypts</subject><subject>Desorption</subject><subject>Epithelium</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Ionization</subject><subject>Ions</subject><subject>Lasers</subject><subject>Liver</subject><subject>Liver - chemistry</subject><subject>Liver - cytology</subject><subject>Liver - pathology</subject><subject>Mass spectra</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Miscellaneous. Technology</subject><subject>Nanotechnology</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Populations</subject><subject>Proteins</subject><subject>Scientific imaging</subject><subject>Sensitivity analysis</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><issn>1044-0305</issn><issn>1879-1123</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkU1r3DAQhkVoSdJtfkKCoKS0B7ejL2t9CiFpk8CGFppDbkKWx6Bgr7caO7D_vtqPEOglJ0nomWHeZxg7FfBNgCi__xGgdQEKzBeQXwHK_Ho8YMdibqtCCKne5fsLcsQ-ED0BCAuVPWRHQmqtQFTH7Pd1TBhG7pe-W1MkPrS884SJB78ap4S8jyENTSTKGDY8YNcRr9f8_nJxfcd7T8Rplf_S0OOY1h_Z-9Z3hCf7c8Yefv54uLotFr9u7q4uF0Uwcz0WNngdjLfa1tKXCKZCDdoYNCgFhBpBokVsEZUwAaqmxGpeClVbqXIONWOfd21Xafg7IY2uj7SZzS9xmMhZWVYlZHjGPv0HPg1TymnJicpkD0IoyJTZUTkrUcLWrVLsfVo7AW7j2219u41MB9JtfbvHXHe27z7VPTavVXvBGTjfA56C79rklyHSK6fzTqQqM3ex4zA7e46YHIWIy4DNdj-uGeIbo_wD3g-bSQ</recordid><startdate>20021101</startdate><enddate>20021101</enddate><creator>Xu, Baogang J</creator><creator>Caprioli, Richard M</creator><creator>Sanders, Melinda E</creator><creator>Jensen, Roy A</creator><general>Elsevier Inc</general><general>Elsevier Science</general><general>Springer Nature B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FG</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>P5Z</scope><scope>P62</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20021101</creationdate><title>Direct analysis of laser capture microdissected cells by MALDI mass spectrometry</title><author>Xu, Baogang J ; Caprioli, Richard M ; Sanders, Melinda E ; Jensen, Roy A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c584t-7ca4c5a747b2a6e059e40455e5e210cbe02e7eefee315c09d6e98613b7230013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Analytical biochemistry: general aspects, technics, instrumentation</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Breast</topic><topic>Breast - chemistry</topic><topic>Breast - cytology</topic><topic>Breast Neoplasms - chemistry</topic><topic>Breast Neoplasms - pathology</topic><topic>Cells - chemistry</topic><topic>Colon</topic><topic>Colon - chemistry</topic><topic>Colon - cytology</topic><topic>Colon - pathology</topic><topic>Crypts</topic><topic>Desorption</topic><topic>Epithelium</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Ionization</topic><topic>Ions</topic><topic>Lasers</topic><topic>Liver</topic><topic>Liver - chemistry</topic><topic>Liver - cytology</topic><topic>Liver - pathology</topic><topic>Mass spectra</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Miscellaneous. Technology</topic><topic>Nanotechnology</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Populations</topic><topic>Proteins</topic><topic>Scientific imaging</topic><topic>Sensitivity analysis</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Baogang J</creatorcontrib><creatorcontrib>Caprioli, Richard M</creatorcontrib><creatorcontrib>Sanders, Melinda E</creatorcontrib><creatorcontrib>Jensen, Roy A</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the American Society for Mass Spectrometry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Baogang J</au><au>Caprioli, Richard M</au><au>Sanders, Melinda E</au><au>Jensen, Roy A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct analysis of laser capture microdissected cells by MALDI mass spectrometry</atitle><jtitle>Journal of the American Society for Mass Spectrometry</jtitle><addtitle>J Am Soc Mass Spectrom</addtitle><date>2002-11-01</date><risdate>2002</risdate><volume>13</volume><issue>11</issue><spage>1292</spage><epage>1297</epage><pages>1292-1297</pages><issn>1044-0305</issn><eissn>1879-1123</eissn><abstract>Laser capture microdissection (LCM) has become an important tool in biological research, permitting isolation of specific cell populations from frozen tissue samples containing a mixture of cell types. Cells obtained by LCM can be directly analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). We report here methodology for the preparation and analysis of LCM captured cells with MALDI MS, giving high sensitivity and mass resolution. Comparison of the spectra obtained from cell populations of interest can identify unique disease or function-related protein markers. Using this approach, mass spectra obtained from human breast tissue containing invasive mammary carcinoma and normal breast epithelium using LCM were compared. Over 40 peaks were identified that significantly differed in intensity between invasive mammary carcinoma and normal breast epithelium. In addition, mass spectra are presented that show protein patterns from mouse liver and mouse colon crypts. The reported tissue preparation procedure and subsequent analysis by MALDI MS provide a new methodology for protein discovery involving LCM captured cells.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>12443019</pmid><doi>10.1016/S1044-0305(02)00644-X</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Analytical biochemistry: general aspects, technics, instrumentation Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Breast Breast - chemistry Breast - cytology Breast Neoplasms - chemistry Breast Neoplasms - pathology Cells - chemistry Colon Colon - chemistry Colon - cytology Colon - pathology Crypts Desorption Epithelium Female Fundamental and applied biological sciences. Psychology Humans Investigative techniques, diagnostic techniques (general aspects) Ionization Ions Lasers Liver Liver - chemistry Liver - cytology Liver - pathology Mass spectra Mass spectrometry Mass spectroscopy Medical sciences Mice Miscellaneous. Technology Nanotechnology Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Populations Proteins Scientific imaging Sensitivity analysis Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| title | Direct analysis of laser capture microdissected cells by MALDI mass spectrometry |
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