Morphological alterations induced by cold-shock in Neurospora crassa
The effects of cold‐shock on Neurospora crassa was here investigated. In cold‐shock treated hyphae (6 h at 4 °C) the branching pattern was changed and dichotomy as well as a fan‐like‐pattern structure were temporarily detected at the tips. When exogenous Ca2+ (500 mM) was added to the hyphae, these...
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| Veröffentlicht in: | Journal of basic microbiology 2002-12, Vol.42 (6), p.381-387 |
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| creator | Kawano, Cristina Yoshiko Said, Suraia |
| description | The effects of cold‐shock on Neurospora crassa was here investigated. In cold‐shock treated hyphae (6 h at 4 °C) the branching pattern was changed and dichotomy as well as a fan‐like‐pattern structure were temporarily detected at the tips. When exogenous Ca2+ (500 mM) was added to the hyphae, these morphological alterations were not detected. In addition, the protein electrophoretic profile of cultures submitted to cold‐shock was different from that of cultures kept at 30 °C. After 1 and 2 hours at 4 °C at least 5 bands were more intense than at 30 °C. The results suggest that cold‐shock temporarily increases the synthesis of some proteins probably involved in the acclimatization process at 4 °C, and that the effects on morphology are probably due to damage to calcium influx. |
| doi_str_mv | 10.1002/1521-4028(200212)42:6<381::AID-JOBM381>3.0.CO;2-8 |
| format | Article |
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Basic Microbiol</addtitle><description>The effects of cold‐shock on Neurospora crassa was here investigated. In cold‐shock treated hyphae (6 h at 4 °C) the branching pattern was changed and dichotomy as well as a fan‐like‐pattern structure were temporarily detected at the tips. When exogenous Ca2+ (500 mM) was added to the hyphae, these morphological alterations were not detected. In addition, the protein electrophoretic profile of cultures submitted to cold‐shock was different from that of cultures kept at 30 °C. After 1 and 2 hours at 4 °C at least 5 bands were more intense than at 30 °C. The results suggest that cold‐shock temporarily increases the synthesis of some proteins probably involved in the acclimatization process at 4 °C, and that the effects on morphology are probably due to damage to calcium influx.</description><subject>Calcium - metabolism</subject><subject>Cold Temperature</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Fungal Proteins - analysis</subject><subject>Hyphae - cytology</subject><subject>Hyphae - growth & development</subject><subject>Mycelium - isolation & purification</subject><subject>Neurospora crassa</subject><subject>Neurospora crassa - chemistry</subject><subject>Neurospora crassa - cytology</subject><subject>Neurospora crassa - growth & development</subject><subject>Time Factors</subject><issn>0233-111X</issn><issn>1521-4028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkUFvEzEQhS1ERUPhL6A9ITg42GOvdx0QUkmhLUobIYHgNvLaXrp0Ewc7K8i_x1HS9IKEOFkzen5P8z1CNGdjzhi84iVwKhnULyCPHF5KmKg3ouaTyenlGf04f3eVh7dizMbT-Wug9QMyOvx5SEYMhKCc82_H5HFKPxhjWoN-RI45SAmCsRE5uwpxdRP68L2zpi9Mv_bRrLuwTEW3dIP1rmg2hQ29o-km2Nu8La79EENahWgKG01K5gk5ak2f_NP9e0K-fHj_eXpBZ_Pzy-npjFoJNaeidQakEUYyJ5VQwqtGWcUq2bBWCKdt5StT29Ior530WgsL1qmmFcCVA3FCnu98VzH8HHxa46JL1ve9WfowJKxAaajL-p9CYCpjqcos_LQT2nxRir7FVewWJm6QM9x2gFueuOWJuw5QAirM2BFzB7jvAAUynM4RcBv-bB8-NAvv7h330MUBz6-u95v_SPx74N0q-9Kdb5fW_vfB18RbVJWoSvx6fY5yBpWEcoZc_AFfyq3g</recordid><startdate>200212</startdate><enddate>200212</enddate><creator>Kawano, Cristina Yoshiko</creator><creator>Said, Suraia</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>200212</creationdate><title>Morphological alterations induced by cold-shock in Neurospora crassa</title><author>Kawano, Cristina Yoshiko ; Said, Suraia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4281-3fda24a3a40d46363e6b6c6074b0f33d9c7e7a8c5a6e9d4e993c2cd6bf3216d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Calcium - metabolism</topic><topic>Cold Temperature</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Fungal Proteins - analysis</topic><topic>Hyphae - cytology</topic><topic>Hyphae - growth & development</topic><topic>Mycelium - isolation & purification</topic><topic>Neurospora crassa</topic><topic>Neurospora crassa - chemistry</topic><topic>Neurospora crassa - cytology</topic><topic>Neurospora crassa - growth & development</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kawano, Cristina Yoshiko</creatorcontrib><creatorcontrib>Said, Suraia</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of basic microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kawano, Cristina Yoshiko</au><au>Said, Suraia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Morphological alterations induced by cold-shock in Neurospora crassa</atitle><jtitle>Journal of basic microbiology</jtitle><addtitle>J. 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| ispartof | Journal of basic microbiology, 2002-12, Vol.42 (6), p.381-387 |
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| language | eng |
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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE |
| subjects | Calcium - metabolism Cold Temperature Electrophoresis, Polyacrylamide Gel Fungal Proteins - analysis Hyphae - cytology Hyphae - growth & development Mycelium - isolation & purification Neurospora crassa Neurospora crassa - chemistry Neurospora crassa - cytology Neurospora crassa - growth & development Time Factors |
| title | Morphological alterations induced by cold-shock in Neurospora crassa |
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