Elastic properties of irradiated RBCs measured by optical tweezers
BACKGROUND : Gamma irradiation of RBCs results in the production of reactive oxygen capable of initiating the process of membrane lipid peroxidation and accelerates the leakage of potassium ions from RBCs, resulting in an increase of internal viscosity. STUDY DESIGN AND METHODS : The elastic propert...
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| Veröffentlicht in: | Transfusion (Philadelphia, Pa.) Pa.), 2002-09, Vol.42 (9), p.1196-1199 |
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| creator | Barjas-Castro, M.L. Brandão, M.M. Fontes, Adriana Costa, F.F. Cesar, C.L. Saad, S.T.O. |
| description | BACKGROUND : Gamma irradiation of RBCs results in the production of reactive oxygen capable of initiating the process of membrane lipid peroxidation and accelerates the leakage of potassium ions from RBCs, resulting in an increase of internal viscosity.
STUDY DESIGN AND METHODS : The elastic properties of irradiated and stored RBC units were studied using laser optical tweezers. The laser trapped the cell and the membrane elasticity was analyzed, measuring the cell deformation in six different drag velocities. Five RBC units were split into two portions. One portion received a gamma irradiation dose of 25 Gy, and the second one was used as a control and was not irradiated. All units were stored (4°C), and the elasticity was examined on Days 1, 14, 21, and 28.
RESULTS
: Elastic properties (μ) from irradiated RBCs stored for 21 and 28 days were significantly affected compared with control cells (21 days: control, 0.3 ± 0.03 × 10
−3
; irradiated, 3.5 ± 1.3 × 10
−3
dyn/cm; p < 0.001; and 28 days: control, 0.5 ± 0.09 × 10
−3
; irradiated, 14 ± 3.2 × 10
−3 dyn/cm; p < 0.001).
CONCLUSION : The sensitivity of the laser optical tweezers method showed that there is no significant change in elasticity over time for up to 14 days of storage, regardless of whether the unit was irradiated or not. However, beyond 21 days of storage, irradiated units demonstrate decreased elasticity. |
| doi_str_mv | 10.1046/j.1537-2995.2002.00201.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72690684</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>72690684</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4321-eae6f19764747a8ea526cc83f6e0743e3952d189a6f4e781e7dd79c78b1346cc3</originalsourceid><addsrcrecordid>eNqNkMtKAzEUhoMotl5eQWajuxlzm2QCbrTeKSql0mVIZ85A6tSpyZS2rgTf1CcxtaXdujjkhHz_yeFDKCI4IZiL81FCUiZjqlSaUIxpEgqTZL6D2puHXdTGmJOYEEZb6MD7EQ6UwmQftQjlDAuZtdH1TWV8Y_No4uoJuMaCj-oyss6ZwpoGiqh31fHRGIyfunAbLqJ6EnhT_Xx9NzOAT3D-CO2VpvJwvD4P0evtTb9zH3ef7x46l90454ySGAyIkigpuOTSZGBSKvI8Y6UALDkDplJakEwZUXKQGQFZFFLlMhsSxgPJDtHZam5Y9mMKvtFj63OoKvMO9dRrSYXCIuMBzFZg7mrvHZR64uzYuIUmWC8N6pFeitJLUXppUP8Z1PMQPVn_MR2OodgG18oCcLoGjA8aSmfec-u3HFOcpkwF7mLFzWwFi38voPu929CEeLyKW9_AfBM37k0LyWSqB093uqMe-aCfEv3CfgHPUps9</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72690684</pqid></control><display><type>article</type><title>Elastic properties of irradiated RBCs measured by optical tweezers</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Barjas-Castro, M.L. ; Brandão, M.M. ; Fontes, Adriana ; Costa, F.F. ; Cesar, C.L. ; Saad, S.T.O.</creator><creatorcontrib>Barjas-Castro, M.L. ; Brandão, M.M. ; Fontes, Adriana ; Costa, F.F. ; Cesar, C.L. ; Saad, S.T.O.</creatorcontrib><description>BACKGROUND : Gamma irradiation of RBCs results in the production of reactive oxygen capable of initiating the process of membrane lipid peroxidation and accelerates the leakage of potassium ions from RBCs, resulting in an increase of internal viscosity.
STUDY DESIGN AND METHODS : The elastic properties of irradiated and stored RBC units were studied using laser optical tweezers. The laser trapped the cell and the membrane elasticity was analyzed, measuring the cell deformation in six different drag velocities. Five RBC units were split into two portions. One portion received a gamma irradiation dose of 25 Gy, and the second one was used as a control and was not irradiated. All units were stored (4°C), and the elasticity was examined on Days 1, 14, 21, and 28.
RESULTS
: Elastic properties (μ) from irradiated RBCs stored for 21 and 28 days were significantly affected compared with control cells (21 days: control, 0.3 ± 0.03 × 10
−3
; irradiated, 3.5 ± 1.3 × 10
−3
dyn/cm; p < 0.001; and 28 days: control, 0.5 ± 0.09 × 10
−3
; irradiated, 14 ± 3.2 × 10
−3 dyn/cm; p < 0.001).
CONCLUSION : The sensitivity of the laser optical tweezers method showed that there is no significant change in elasticity over time for up to 14 days of storage, regardless of whether the unit was irradiated or not. However, beyond 21 days of storage, irradiated units demonstrate decreased elasticity.</description><identifier>ISSN: 0041-1132</identifier><identifier>EISSN: 1537-2995</identifier><identifier>DOI: 10.1046/j.1537-2995.2002.00201.x</identifier><identifier>PMID: 12430678</identifier><identifier>CODEN: TRANAT</identifier><language>eng</language><publisher>Boston, MA, USA: Blackwell Science Inc</publisher><subject>Adult ; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Biological and medical sciences ; Blood Preservation ; Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis ; Elasticity ; Erythrocyte Deformability - radiation effects ; Erythrocyte Membrane - radiation effects ; Humans ; Lipid Peroxidation ; Medical sciences ; Membrane Lipids - radiation effects ; Micromanipulation - instrumentation ; Time Factors ; Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><ispartof>Transfusion (Philadelphia, Pa.), 2002-09, Vol.42 (9), p.1196-1199</ispartof><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4321-eae6f19764747a8ea526cc83f6e0743e3952d189a6f4e781e7dd79c78b1346cc3</citedby><cites>FETCH-LOGICAL-c4321-eae6f19764747a8ea526cc83f6e0743e3952d189a6f4e781e7dd79c78b1346cc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1537-2995.2002.00201.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1537-2995.2002.00201.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27929,27930,45579,45580</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13942539$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12430678$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Barjas-Castro, M.L.</creatorcontrib><creatorcontrib>Brandão, M.M.</creatorcontrib><creatorcontrib>Fontes, Adriana</creatorcontrib><creatorcontrib>Costa, F.F.</creatorcontrib><creatorcontrib>Cesar, C.L.</creatorcontrib><creatorcontrib>Saad, S.T.O.</creatorcontrib><title>Elastic properties of irradiated RBCs measured by optical tweezers</title><title>Transfusion (Philadelphia, Pa.)</title><addtitle>Transfusion</addtitle><description>BACKGROUND : Gamma irradiation of RBCs results in the production of reactive oxygen capable of initiating the process of membrane lipid peroxidation and accelerates the leakage of potassium ions from RBCs, resulting in an increase of internal viscosity.
STUDY DESIGN AND METHODS : The elastic properties of irradiated and stored RBC units were studied using laser optical tweezers. The laser trapped the cell and the membrane elasticity was analyzed, measuring the cell deformation in six different drag velocities. Five RBC units were split into two portions. One portion received a gamma irradiation dose of 25 Gy, and the second one was used as a control and was not irradiated. All units were stored (4°C), and the elasticity was examined on Days 1, 14, 21, and 28.
RESULTS
: Elastic properties (μ) from irradiated RBCs stored for 21 and 28 days were significantly affected compared with control cells (21 days: control, 0.3 ± 0.03 × 10
−3
; irradiated, 3.5 ± 1.3 × 10
−3
dyn/cm; p < 0.001; and 28 days: control, 0.5 ± 0.09 × 10
−3
; irradiated, 14 ± 3.2 × 10
−3 dyn/cm; p < 0.001).
CONCLUSION : The sensitivity of the laser optical tweezers method showed that there is no significant change in elasticity over time for up to 14 days of storage, regardless of whether the unit was irradiated or not. However, beyond 21 days of storage, irradiated units demonstrate decreased elasticity.</description><subject>Adult</subject><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Biological and medical sciences</subject><subject>Blood Preservation</subject><subject>Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis</subject><subject>Elasticity</subject><subject>Erythrocyte Deformability - radiation effects</subject><subject>Erythrocyte Membrane - radiation effects</subject><subject>Humans</subject><subject>Lipid Peroxidation</subject><subject>Medical sciences</subject><subject>Membrane Lipids - radiation effects</subject><subject>Micromanipulation - instrumentation</subject><subject>Time Factors</subject><subject>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><issn>0041-1132</issn><issn>1537-2995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMtKAzEUhoMotl5eQWajuxlzm2QCbrTeKSql0mVIZ85A6tSpyZS2rgTf1CcxtaXdujjkhHz_yeFDKCI4IZiL81FCUiZjqlSaUIxpEgqTZL6D2puHXdTGmJOYEEZb6MD7EQ6UwmQftQjlDAuZtdH1TWV8Y_No4uoJuMaCj-oyss6ZwpoGiqh31fHRGIyfunAbLqJ6EnhT_Xx9NzOAT3D-CO2VpvJwvD4P0evtTb9zH3ef7x46l90454ySGAyIkigpuOTSZGBSKvI8Y6UALDkDplJakEwZUXKQGQFZFFLlMhsSxgPJDtHZam5Y9mMKvtFj63OoKvMO9dRrSYXCIuMBzFZg7mrvHZR64uzYuIUmWC8N6pFeitJLUXppUP8Z1PMQPVn_MR2OodgG18oCcLoGjA8aSmfec-u3HFOcpkwF7mLFzWwFi38voPu929CEeLyKW9_AfBM37k0LyWSqB093uqMe-aCfEv3CfgHPUps9</recordid><startdate>200209</startdate><enddate>200209</enddate><creator>Barjas-Castro, M.L.</creator><creator>Brandão, M.M.</creator><creator>Fontes, Adriana</creator><creator>Costa, F.F.</creator><creator>Cesar, C.L.</creator><creator>Saad, S.T.O.</creator><general>Blackwell Science Inc</general><general>Blackwell Publishing</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200209</creationdate><title>Elastic properties of irradiated RBCs measured by optical tweezers</title><author>Barjas-Castro, M.L. ; Brandão, M.M. ; Fontes, Adriana ; Costa, F.F. ; Cesar, C.L. ; Saad, S.T.O.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4321-eae6f19764747a8ea526cc83f6e0743e3952d189a6f4e781e7dd79c78b1346cc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adult</topic><topic>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Biological and medical sciences</topic><topic>Blood Preservation</topic><topic>Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis</topic><topic>Elasticity</topic><topic>Erythrocyte Deformability - radiation effects</topic><topic>Erythrocyte Membrane - radiation effects</topic><topic>Humans</topic><topic>Lipid Peroxidation</topic><topic>Medical sciences</topic><topic>Membrane Lipids - radiation effects</topic><topic>Micromanipulation - instrumentation</topic><topic>Time Factors</topic><topic>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barjas-Castro, M.L.</creatorcontrib><creatorcontrib>Brandão, M.M.</creatorcontrib><creatorcontrib>Fontes, Adriana</creatorcontrib><creatorcontrib>Costa, F.F.</creatorcontrib><creatorcontrib>Cesar, C.L.</creatorcontrib><creatorcontrib>Saad, S.T.O.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Transfusion (Philadelphia, Pa.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barjas-Castro, M.L.</au><au>Brandão, M.M.</au><au>Fontes, Adriana</au><au>Costa, F.F.</au><au>Cesar, C.L.</au><au>Saad, S.T.O.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Elastic properties of irradiated RBCs measured by optical tweezers</atitle><jtitle>Transfusion (Philadelphia, Pa.)</jtitle><addtitle>Transfusion</addtitle><date>2002-09</date><risdate>2002</risdate><volume>42</volume><issue>9</issue><spage>1196</spage><epage>1199</epage><pages>1196-1199</pages><issn>0041-1132</issn><eissn>1537-2995</eissn><coden>TRANAT</coden><abstract>BACKGROUND : Gamma irradiation of RBCs results in the production of reactive oxygen capable of initiating the process of membrane lipid peroxidation and accelerates the leakage of potassium ions from RBCs, resulting in an increase of internal viscosity.
STUDY DESIGN AND METHODS : The elastic properties of irradiated and stored RBC units were studied using laser optical tweezers. The laser trapped the cell and the membrane elasticity was analyzed, measuring the cell deformation in six different drag velocities. Five RBC units were split into two portions. One portion received a gamma irradiation dose of 25 Gy, and the second one was used as a control and was not irradiated. All units were stored (4°C), and the elasticity was examined on Days 1, 14, 21, and 28.
RESULTS
: Elastic properties (μ) from irradiated RBCs stored for 21 and 28 days were significantly affected compared with control cells (21 days: control, 0.3 ± 0.03 × 10
−3
; irradiated, 3.5 ± 1.3 × 10
−3
dyn/cm; p < 0.001; and 28 days: control, 0.5 ± 0.09 × 10
−3
; irradiated, 14 ± 3.2 × 10
−3 dyn/cm; p < 0.001).
CONCLUSION : The sensitivity of the laser optical tweezers method showed that there is no significant change in elasticity over time for up to 14 days of storage, regardless of whether the unit was irradiated or not. However, beyond 21 days of storage, irradiated units demonstrate decreased elasticity.</abstract><cop>Boston, MA, USA</cop><pub>Blackwell Science Inc</pub><pmid>12430678</pmid><doi>10.1046/j.1537-2995.2002.00201.x</doi><tpages>4</tpages></addata></record> |
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| subjects | Adult Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Biological and medical sciences Blood Preservation Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis Elasticity Erythrocyte Deformability - radiation effects Erythrocyte Membrane - radiation effects Humans Lipid Peroxidation Medical sciences Membrane Lipids - radiation effects Micromanipulation - instrumentation Time Factors Transfusions. Complications. Transfusion reactions. Cell and gene therapy |
| title | Elastic properties of irradiated RBCs measured by optical tweezers |
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