Functional assay of protein S in 70 patients with congenital and acquired disorders
A functional assay for the selective measurement of the active form of protein S in plasma, based on the prolongation of an APTT, was previously developed. This assay is sensitive, reproducible and specific, not affected by other clotting factors including FVIII. This method was applied to the measu...
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| Veröffentlicht in: | Blood coagulation & fibrinolysis 1991-12, Vol.2 (6), p.705-712 |
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| container_title | Blood coagulation & fibrinolysis |
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| creator | Wolf, M Boyer-Neumann, C Leroy-Matheron, C Martinoli, J L Contant, G Amiral, J Meyer, D Larrieu, M J |
| description | A functional assay for the selective measurement of the active form of protein S in plasma, based on the prolongation of an APTT, was previously developed. This assay is sensitive, reproducible and specific, not affected by other clotting factors including FVIII. This method was applied to the measurement of protein S activity in congenital and acquired disorders. Results of protein S activity were compared to those of total and free antigen measured by ELISA. In 30 controls, there was an excellent correlation between protein S activity and free antigen. In patients with inflammatory disease, protein S activity and free antigen were normal, despite high levels of both C4b-binding protein and total protein S antigen. In dicoumarol-treated patients, protein S activity was lower than free antigen due to the presence of acarboxylated forms. Surprisingly, in liver cirrhosis, free antigen was only slightly decreased whereas protein S activity was significantly reduced. In 23 patients with congenital deficiency, protein S activity was consistently decreased, from < 5% to 60% and showed good correlation with the free antigen. This functional assay allows the rapid diagnosis of congenital or acquired deficiency of protein S. |
| doi_str_mv | 10.1097/00001721-199112000-00003 |
| format | Article |
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This assay is sensitive, reproducible and specific, not affected by other clotting factors including FVIII. This method was applied to the measurement of protein S activity in congenital and acquired disorders. Results of protein S activity were compared to those of total and free antigen measured by ELISA. In 30 controls, there was an excellent correlation between protein S activity and free antigen. In patients with inflammatory disease, protein S activity and free antigen were normal, despite high levels of both C4b-binding protein and total protein S antigen. In dicoumarol-treated patients, protein S activity was lower than free antigen due to the presence of acarboxylated forms. Surprisingly, in liver cirrhosis, free antigen was only slightly decreased whereas protein S activity was significantly reduced. In 23 patients with congenital deficiency, protein S activity was consistently decreased, from < 5% to 60% and showed good correlation with the free antigen. This functional assay allows the rapid diagnosis of congenital or acquired deficiency of protein S.</description><identifier>ISSN: 0957-5235</identifier><identifier>EISSN: 1473-5733</identifier><identifier>DOI: 10.1097/00001721-199112000-00003</identifier><identifier>PMID: 1839222</identifier><language>eng</language><publisher>England: Lippincott-Raven Publishers</publisher><subject>Adult ; Carrier Proteins - isolation & purification ; Complement Inactivator Proteins ; Enzyme-Linked Immunosorbent Assay ; Factor Va - metabolism ; Female ; Genetic Diseases, Inborn - blood ; Glycoproteins - blood ; Glycoproteins - deficiency ; Glycoproteins - isolation & purification ; Humans ; Inflammation - blood ; Liver Diseases - blood ; Male ; Middle Aged ; Partial Thromboplastin Time ; Pedigree ; Protein C - metabolism ; Protein S</subject><ispartof>Blood coagulation & fibrinolysis, 1991-12, Vol.2 (6), p.705-712</ispartof><rights>Lippincott-Raven Publishers.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3553-340e5f5120c1e34dbaf9c11753c426f8a7db32c059b788be1fe82c6ce343918c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1839222$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wolf, M</creatorcontrib><creatorcontrib>Boyer-Neumann, C</creatorcontrib><creatorcontrib>Leroy-Matheron, C</creatorcontrib><creatorcontrib>Martinoli, J L</creatorcontrib><creatorcontrib>Contant, G</creatorcontrib><creatorcontrib>Amiral, J</creatorcontrib><creatorcontrib>Meyer, D</creatorcontrib><creatorcontrib>Larrieu, M J</creatorcontrib><title>Functional assay of protein S in 70 patients with congenital and acquired disorders</title><title>Blood coagulation & fibrinolysis</title><addtitle>Blood Coagul Fibrinolysis</addtitle><description>A functional assay for the selective measurement of the active form of protein S in plasma, based on the prolongation of an APTT, was previously developed. This assay is sensitive, reproducible and specific, not affected by other clotting factors including FVIII. This method was applied to the measurement of protein S activity in congenital and acquired disorders. Results of protein S activity were compared to those of total and free antigen measured by ELISA. In 30 controls, there was an excellent correlation between protein S activity and free antigen. In patients with inflammatory disease, protein S activity and free antigen were normal, despite high levels of both C4b-binding protein and total protein S antigen. In dicoumarol-treated patients, protein S activity was lower than free antigen due to the presence of acarboxylated forms. Surprisingly, in liver cirrhosis, free antigen was only slightly decreased whereas protein S activity was significantly reduced. In 23 patients with congenital deficiency, protein S activity was consistently decreased, from < 5% to 60% and showed good correlation with the free antigen. This functional assay allows the rapid diagnosis of congenital or acquired deficiency of protein S.</description><subject>Adult</subject><subject>Carrier Proteins - isolation & purification</subject><subject>Complement Inactivator Proteins</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Factor Va - metabolism</subject><subject>Female</subject><subject>Genetic Diseases, Inborn - blood</subject><subject>Glycoproteins - blood</subject><subject>Glycoproteins - deficiency</subject><subject>Glycoproteins - isolation & purification</subject><subject>Humans</subject><subject>Inflammation - blood</subject><subject>Liver Diseases - blood</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Partial Thromboplastin Time</subject><subject>Pedigree</subject><subject>Protein C - metabolism</subject><subject>Protein S</subject><issn>0957-5235</issn><issn>1473-5733</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1UcFOAyEQJUZTa_UTTDh5W11gKezRNFZNmniongnLzlp0y7bApunfy9qqJzkM4c17w8wbhDDJb0leirs8HSIoyUhZEkLTKxsgdoLGpBAs44KxUzTOSy4yThk_RxchfAyMQooRGhHJSkrpGC3nvTPRdk63WIeg97hr8MZ3EazDS5yCyPFGRwsuBryzcYVN597B2TgoXI212fbWQ41rGzpfgw-X6KzRbYCr4z1Bb_OH19lTtnh5fJ7dLzLDOGcZK3LgDU_dGwKsqCvdlIYQwZkp6LSRWtQVoybnZSWkrIA0IKmZmsRlJZGGTdDNoW7qd9tDiGptg4G21Q66PihBp1KkSRNRHojGdyF4aNTG27X2e0VyNfipfvxUv35-Q4P0-vhHX62h_hMeDEz54pDfdW1Ms3-2_Q68WoFu40r9tyb2BQKtf2Y</recordid><startdate>199112</startdate><enddate>199112</enddate><creator>Wolf, M</creator><creator>Boyer-Neumann, C</creator><creator>Leroy-Matheron, C</creator><creator>Martinoli, J L</creator><creator>Contant, G</creator><creator>Amiral, J</creator><creator>Meyer, D</creator><creator>Larrieu, M J</creator><general>Lippincott-Raven Publishers</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199112</creationdate><title>Functional assay of protein S in 70 patients with congenital and acquired disorders</title><author>Wolf, M ; Boyer-Neumann, C ; Leroy-Matheron, C ; Martinoli, J L ; Contant, G ; Amiral, J ; Meyer, D ; Larrieu, M J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3553-340e5f5120c1e34dbaf9c11753c426f8a7db32c059b788be1fe82c6ce343918c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Adult</topic><topic>Carrier Proteins - isolation & purification</topic><topic>Complement Inactivator Proteins</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Factor Va - metabolism</topic><topic>Female</topic><topic>Genetic Diseases, Inborn - blood</topic><topic>Glycoproteins - blood</topic><topic>Glycoproteins - deficiency</topic><topic>Glycoproteins - isolation & purification</topic><topic>Humans</topic><topic>Inflammation - blood</topic><topic>Liver Diseases - blood</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Partial Thromboplastin Time</topic><topic>Pedigree</topic><topic>Protein C - metabolism</topic><topic>Protein S</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wolf, M</creatorcontrib><creatorcontrib>Boyer-Neumann, C</creatorcontrib><creatorcontrib>Leroy-Matheron, C</creatorcontrib><creatorcontrib>Martinoli, J L</creatorcontrib><creatorcontrib>Contant, G</creatorcontrib><creatorcontrib>Amiral, J</creatorcontrib><creatorcontrib>Meyer, D</creatorcontrib><creatorcontrib>Larrieu, M J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Blood coagulation & fibrinolysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wolf, M</au><au>Boyer-Neumann, C</au><au>Leroy-Matheron, C</au><au>Martinoli, J L</au><au>Contant, G</au><au>Amiral, J</au><au>Meyer, D</au><au>Larrieu, M J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional assay of protein S in 70 patients with congenital and acquired disorders</atitle><jtitle>Blood coagulation & fibrinolysis</jtitle><addtitle>Blood Coagul Fibrinolysis</addtitle><date>1991-12</date><risdate>1991</risdate><volume>2</volume><issue>6</issue><spage>705</spage><epage>712</epage><pages>705-712</pages><issn>0957-5235</issn><eissn>1473-5733</eissn><abstract>A functional assay for the selective measurement of the active form of protein S in plasma, based on the prolongation of an APTT, was previously developed. This assay is sensitive, reproducible and specific, not affected by other clotting factors including FVIII. This method was applied to the measurement of protein S activity in congenital and acquired disorders. Results of protein S activity were compared to those of total and free antigen measured by ELISA. In 30 controls, there was an excellent correlation between protein S activity and free antigen. In patients with inflammatory disease, protein S activity and free antigen were normal, despite high levels of both C4b-binding protein and total protein S antigen. In dicoumarol-treated patients, protein S activity was lower than free antigen due to the presence of acarboxylated forms. Surprisingly, in liver cirrhosis, free antigen was only slightly decreased whereas protein S activity was significantly reduced. In 23 patients with congenital deficiency, protein S activity was consistently decreased, from < 5% to 60% and showed good correlation with the free antigen. This functional assay allows the rapid diagnosis of congenital or acquired deficiency of protein S.</abstract><cop>England</cop><pub>Lippincott-Raven Publishers</pub><pmid>1839222</pmid><doi>10.1097/00001721-199112000-00003</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0957-5235 |
| ispartof | Blood coagulation & fibrinolysis, 1991-12, Vol.2 (6), p.705-712 |
| issn | 0957-5235 1473-5733 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72687183 |
| source | MEDLINE; Journals@Ovid Complete |
| subjects | Adult Carrier Proteins - isolation & purification Complement Inactivator Proteins Enzyme-Linked Immunosorbent Assay Factor Va - metabolism Female Genetic Diseases, Inborn - blood Glycoproteins - blood Glycoproteins - deficiency Glycoproteins - isolation & purification Humans Inflammation - blood Liver Diseases - blood Male Middle Aged Partial Thromboplastin Time Pedigree Protein C - metabolism Protein S |
| title | Functional assay of protein S in 70 patients with congenital and acquired disorders |
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