Platelet-derived growth factor-D expression in developing and mature human kidneys
Platelet-derived growth factor-D expression in developing and mature human kidneys. Platelet-derived growth factor (PDGF) is a family of growth regulatory molecules composed of sulfide-bonded dimeric structures. Two well-studied PDGF peptides (PDGF-A and PDGF-B) have been shown to mediate a wide ran...
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description | Platelet-derived growth factor-D expression in developing and mature human kidneys.
Platelet-derived growth factor (PDGF) is a family of growth regulatory molecules composed of sulfide-bonded dimeric structures. Two well-studied PDGF peptides (PDGF-A and PDGF-B) have been shown to mediate a wide range of biological effects. PDGF-D is a newly recognized member of the PDGF family. Initial studies of the PDGF-D gene found its expression in cells of the vascular wall, suggesting that it could participate in vascular development and pathology. However, its localization in human kidney tissues has never been studied.
PDGF-D expression in fetal (N = 30) and adult (N = 25) human kidney tissues was examined by immunohistochemistry using an affinity-purified antibody raised to human PDGF-D. Antibody absorption with the immunizing peptide was employed to confirm the specificity of this antibody. PDGF-D protein and gene expression in human kidneys also were demonstrated by Western blotting and reverse transcription-polymerase chain reaction (RT-PCR).
In the developing kidney, PDGF-D was first expressed by epithelial cells of comma- and S-shaped structures of the developing nephron, and most consistently in the visceral epithelial cells in the later stages of glomerular differentiation. In addition, PDGF-D could be found in mesenchymal, presumptively fibroblast cells in the interstitium of developing renal pelvis and in fetal smooth muscle cells in arterial vessels. In the adult normal kidney, PDGF-D was expressed by the visceral epithelial cells. There was persistent expression in arterial smooth muscle cells as well as in some neointimal smooth muscle cells of arteriosclerotic vessels, and expression in smooth muscle cells of vasa rectae in the medulla. PDGF-D could be identified at the basolateral membrane of some injured tubules in areas of chronic tubulointerstitial injury routinely encountered in aging kidneys. Western blotting of homogenates of adult kidneys demonstrated monospecific bands at 50 kD corresponding to previously established size parameter for this protein. RT-PCR of human kidney RNA resulted in a 918 basepair band, the sequence of which corresponded to human PDGF-D (Genbank number AF336376).
To our knowledge, these are the first studies to localize PDGF-D in human kidneys and suggest that PDGF-D may have a role in kidney development. PDGF-D was shown to bind to PDGF β receptor, which localizes to mesangial cells, parietal epithelial cells, and inte |
doi_str_mv | 10.1046/j.1523-1755.2002.00662.x |
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Platelet-derived growth factor (PDGF) is a family of growth regulatory molecules composed of sulfide-bonded dimeric structures. Two well-studied PDGF peptides (PDGF-A and PDGF-B) have been shown to mediate a wide range of biological effects. PDGF-D is a newly recognized member of the PDGF family. Initial studies of the PDGF-D gene found its expression in cells of the vascular wall, suggesting that it could participate in vascular development and pathology. However, its localization in human kidney tissues has never been studied.
PDGF-D expression in fetal (N = 30) and adult (N = 25) human kidney tissues was examined by immunohistochemistry using an affinity-purified antibody raised to human PDGF-D. Antibody absorption with the immunizing peptide was employed to confirm the specificity of this antibody. PDGF-D protein and gene expression in human kidneys also were demonstrated by Western blotting and reverse transcription-polymerase chain reaction (RT-PCR).
In the developing kidney, PDGF-D was first expressed by epithelial cells of comma- and S-shaped structures of the developing nephron, and most consistently in the visceral epithelial cells in the later stages of glomerular differentiation. In addition, PDGF-D could be found in mesenchymal, presumptively fibroblast cells in the interstitium of developing renal pelvis and in fetal smooth muscle cells in arterial vessels. In the adult normal kidney, PDGF-D was expressed by the visceral epithelial cells. There was persistent expression in arterial smooth muscle cells as well as in some neointimal smooth muscle cells of arteriosclerotic vessels, and expression in smooth muscle cells of vasa rectae in the medulla. PDGF-D could be identified at the basolateral membrane of some injured tubules in areas of chronic tubulointerstitial injury routinely encountered in aging kidneys. Western blotting of homogenates of adult kidneys demonstrated monospecific bands at 50 kD corresponding to previously established size parameter for this protein. RT-PCR of human kidney RNA resulted in a 918 basepair band, the sequence of which corresponded to human PDGF-D (Genbank number AF336376).
To our knowledge, these are the first studies to localize PDGF-D in human kidneys and suggest that PDGF-D may have a role in kidney development. PDGF-D was shown to bind to PDGF β receptor, which localizes to mesangial cells, parietal epithelial cells, and interstitial fibroblasts, suggesting potential paracrine interactions between those cells and the visceral epithelium.</description><identifier>ISSN: 0085-2538</identifier><identifier>EISSN: 1523-1755</identifier><identifier>DOI: 10.1046/j.1523-1755.2002.00662.x</identifier><identifier>PMID: 12427128</identifier><identifier>CODEN: KDYIA5</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Adult ; Antibody Specificity ; Biological and medical sciences ; Blotting, Western ; developing nephron ; Fetus - chemistry ; fibroblasts ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Developmental ; glomerulogenesis arteriosclerosis ; growth regulatory molecule ; Humans ; Immunohistochemistry ; Kidney - chemistry ; Kidney - embryology ; Kidney - physiology ; Lymphokines ; Molecular Sequence Data ; platelet-derived growth factor ; Platelet-Derived Growth Factor - analysis ; Platelet-Derived Growth Factor - genetics ; Platelet-Derived Growth Factor - immunology ; protein PDGF-D ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - analysis ; vascular development ; Vertebrates: urinary system</subject><ispartof>Kidney international, 2002-12, Vol.62 (6), p.2043-2054</ispartof><rights>2002 International Society of Nephrology</rights><rights>2003 INIST-CNRS</rights><rights>Copyright Nature Publishing Group Dec 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c565t-b41ee71807000371fabbdc46192fc405c06dd828f579289669ef4bc5d19ebd413</citedby><cites>FETCH-LOGICAL-c565t-b41ee71807000371fabbdc46192fc405c06dd828f579289669ef4bc5d19ebd413</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/210123490?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,64385,64387,64389,72469</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14023899$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12427128$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Changsirikulchai, Siribha</creatorcontrib><creatorcontrib>Hudkins, Kelly L.</creatorcontrib><creatorcontrib>Goodpaster, Tracy A.</creatorcontrib><creatorcontrib>Volpone, John</creatorcontrib><creatorcontrib>Topouzis, Stavros</creatorcontrib><creatorcontrib>Gilbertson, Debra G.</creatorcontrib><creatorcontrib>Alpers, Charles E.</creatorcontrib><title>Platelet-derived growth factor-D expression in developing and mature human kidneys</title><title>Kidney international</title><addtitle>Kidney Int</addtitle><description>Platelet-derived growth factor-D expression in developing and mature human kidneys.
Platelet-derived growth factor (PDGF) is a family of growth regulatory molecules composed of sulfide-bonded dimeric structures. Two well-studied PDGF peptides (PDGF-A and PDGF-B) have been shown to mediate a wide range of biological effects. PDGF-D is a newly recognized member of the PDGF family. Initial studies of the PDGF-D gene found its expression in cells of the vascular wall, suggesting that it could participate in vascular development and pathology. However, its localization in human kidney tissues has never been studied.
PDGF-D expression in fetal (N = 30) and adult (N = 25) human kidney tissues was examined by immunohistochemistry using an affinity-purified antibody raised to human PDGF-D. Antibody absorption with the immunizing peptide was employed to confirm the specificity of this antibody. PDGF-D protein and gene expression in human kidneys also were demonstrated by Western blotting and reverse transcription-polymerase chain reaction (RT-PCR).
In the developing kidney, PDGF-D was first expressed by epithelial cells of comma- and S-shaped structures of the developing nephron, and most consistently in the visceral epithelial cells in the later stages of glomerular differentiation. In addition, PDGF-D could be found in mesenchymal, presumptively fibroblast cells in the interstitium of developing renal pelvis and in fetal smooth muscle cells in arterial vessels. In the adult normal kidney, PDGF-D was expressed by the visceral epithelial cells. There was persistent expression in arterial smooth muscle cells as well as in some neointimal smooth muscle cells of arteriosclerotic vessels, and expression in smooth muscle cells of vasa rectae in the medulla. PDGF-D could be identified at the basolateral membrane of some injured tubules in areas of chronic tubulointerstitial injury routinely encountered in aging kidneys. Western blotting of homogenates of adult kidneys demonstrated monospecific bands at 50 kD corresponding to previously established size parameter for this protein. RT-PCR of human kidney RNA resulted in a 918 basepair band, the sequence of which corresponded to human PDGF-D (Genbank number AF336376).
To our knowledge, these are the first studies to localize PDGF-D in human kidneys and suggest that PDGF-D may have a role in kidney development. PDGF-D was shown to bind to PDGF β receptor, which localizes to mesangial cells, parietal epithelial cells, and interstitial fibroblasts, suggesting potential paracrine interactions between those cells and the visceral epithelium.</description><subject>Adult</subject><subject>Antibody Specificity</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>developing nephron</subject><subject>Fetus - chemistry</subject><subject>fibroblasts</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Developmental</subject><subject>glomerulogenesis arteriosclerosis</subject><subject>growth regulatory molecule</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Kidney - chemistry</subject><subject>Kidney - embryology</subject><subject>Kidney - physiology</subject><subject>Lymphokines</subject><subject>Molecular Sequence Data</subject><subject>platelet-derived growth factor</subject><subject>Platelet-Derived Growth Factor - analysis</subject><subject>Platelet-Derived Growth Factor - genetics</subject><subject>Platelet-Derived Growth Factor - immunology</subject><subject>protein PDGF-D</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - analysis</subject><subject>vascular development</subject><subject>Vertebrates: urinary system</subject><issn>0085-2538</issn><issn>1523-1755</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqFkE1v1DAQhi1ERZfCTwBZSHBLaju2Yx-hfFWqVITgbDn2pPWSOIudLNt_j7e7aiUunEaj95nRqwchTElNCZfn65oK1lS0FaJmhLCaEClZvXuCVg_BU7QiRImKiUadouc5r0nZdUOeoVPKOGspUyv0_dtgZxhgrjyksAWPb9L0Z77FvXXzlKqPGHabBDmHKeIQsYctDNMmxBtso8ejnZcE-HYZbcS_go9wl1-gk94OGV4e5xn6-fnTj4uv1dX1l8uL91eVE1LMVccpQEsVaUuvpqW97TrvuKSa9Y4T4Yj0XjHVi1YzpaXU0PPOCU81dJ7T5gy9O_zdpOn3Ank2Y8gOhsFGmJZsWiaVVG1bwDf_gOtpSbF0M4wSyhquSYHUAXJpyjlBbzYpjDbdGUrMXrpZm71bs3dr9tLNvXSzK6evj_-XbgT_eHi0XIC3R8BmZ4c-2ehCfuQ4YY3SunCvDly81_oAcK6bhvGSfzjkULRuAySTXYDowIcEbjZ-Cv9v-xdghalZ</recordid><startdate>20021201</startdate><enddate>20021201</enddate><creator>Changsirikulchai, Siribha</creator><creator>Hudkins, Kelly L.</creator><creator>Goodpaster, Tracy A.</creator><creator>Volpone, John</creator><creator>Topouzis, Stavros</creator><creator>Gilbertson, Debra G.</creator><creator>Alpers, Charles E.</creator><general>Elsevier Inc</general><general>Nature Publishing</general><general>Elsevier Limited</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20021201</creationdate><title>Platelet-derived growth factor-D expression in developing and mature human kidneys</title><author>Changsirikulchai, Siribha ; Hudkins, Kelly L. ; Goodpaster, Tracy A. ; Volpone, John ; Topouzis, Stavros ; Gilbertson, Debra G. ; Alpers, Charles E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c565t-b41ee71807000371fabbdc46192fc405c06dd828f579289669ef4bc5d19ebd413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adult</topic><topic>Antibody Specificity</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>developing nephron</topic><topic>Fetus - chemistry</topic><topic>fibroblasts</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Developmental</topic><topic>glomerulogenesis arteriosclerosis</topic><topic>growth regulatory molecule</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Kidney - chemistry</topic><topic>Kidney - embryology</topic><topic>Kidney - physiology</topic><topic>Lymphokines</topic><topic>Molecular Sequence Data</topic><topic>platelet-derived growth factor</topic><topic>Platelet-Derived Growth Factor - analysis</topic><topic>Platelet-Derived Growth Factor - genetics</topic><topic>Platelet-Derived Growth Factor - immunology</topic><topic>protein PDGF-D</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - analysis</topic><topic>vascular development</topic><topic>Vertebrates: urinary system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Changsirikulchai, Siribha</creatorcontrib><creatorcontrib>Hudkins, Kelly L.</creatorcontrib><creatorcontrib>Goodpaster, Tracy A.</creatorcontrib><creatorcontrib>Volpone, John</creatorcontrib><creatorcontrib>Topouzis, Stavros</creatorcontrib><creatorcontrib>Gilbertson, Debra G.</creatorcontrib><creatorcontrib>Alpers, Charles E.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Kidney international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Changsirikulchai, Siribha</au><au>Hudkins, Kelly L.</au><au>Goodpaster, Tracy A.</au><au>Volpone, John</au><au>Topouzis, Stavros</au><au>Gilbertson, Debra G.</au><au>Alpers, Charles E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Platelet-derived growth factor-D expression in developing and mature human kidneys</atitle><jtitle>Kidney international</jtitle><addtitle>Kidney Int</addtitle><date>2002-12-01</date><risdate>2002</risdate><volume>62</volume><issue>6</issue><spage>2043</spage><epage>2054</epage><pages>2043-2054</pages><issn>0085-2538</issn><eissn>1523-1755</eissn><coden>KDYIA5</coden><abstract>Platelet-derived growth factor-D expression in developing and mature human kidneys.
Platelet-derived growth factor (PDGF) is a family of growth regulatory molecules composed of sulfide-bonded dimeric structures. Two well-studied PDGF peptides (PDGF-A and PDGF-B) have been shown to mediate a wide range of biological effects. PDGF-D is a newly recognized member of the PDGF family. Initial studies of the PDGF-D gene found its expression in cells of the vascular wall, suggesting that it could participate in vascular development and pathology. However, its localization in human kidney tissues has never been studied.
PDGF-D expression in fetal (N = 30) and adult (N = 25) human kidney tissues was examined by immunohistochemistry using an affinity-purified antibody raised to human PDGF-D. Antibody absorption with the immunizing peptide was employed to confirm the specificity of this antibody. PDGF-D protein and gene expression in human kidneys also were demonstrated by Western blotting and reverse transcription-polymerase chain reaction (RT-PCR).
In the developing kidney, PDGF-D was first expressed by epithelial cells of comma- and S-shaped structures of the developing nephron, and most consistently in the visceral epithelial cells in the later stages of glomerular differentiation. In addition, PDGF-D could be found in mesenchymal, presumptively fibroblast cells in the interstitium of developing renal pelvis and in fetal smooth muscle cells in arterial vessels. In the adult normal kidney, PDGF-D was expressed by the visceral epithelial cells. There was persistent expression in arterial smooth muscle cells as well as in some neointimal smooth muscle cells of arteriosclerotic vessels, and expression in smooth muscle cells of vasa rectae in the medulla. PDGF-D could be identified at the basolateral membrane of some injured tubules in areas of chronic tubulointerstitial injury routinely encountered in aging kidneys. Western blotting of homogenates of adult kidneys demonstrated monospecific bands at 50 kD corresponding to previously established size parameter for this protein. RT-PCR of human kidney RNA resulted in a 918 basepair band, the sequence of which corresponded to human PDGF-D (Genbank number AF336376).
To our knowledge, these are the first studies to localize PDGF-D in human kidneys and suggest that PDGF-D may have a role in kidney development. PDGF-D was shown to bind to PDGF β receptor, which localizes to mesangial cells, parietal epithelial cells, and interstitial fibroblasts, suggesting potential paracrine interactions between those cells and the visceral epithelium.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>12427128</pmid><doi>10.1046/j.1523-1755.2002.00662.x</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Adult Antibody Specificity Biological and medical sciences Blotting, Western developing nephron Fetus - chemistry fibroblasts Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Developmental glomerulogenesis arteriosclerosis growth regulatory molecule Humans Immunohistochemistry Kidney - chemistry Kidney - embryology Kidney - physiology Lymphokines Molecular Sequence Data platelet-derived growth factor Platelet-Derived Growth Factor - analysis Platelet-Derived Growth Factor - genetics Platelet-Derived Growth Factor - immunology protein PDGF-D Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - analysis vascular development Vertebrates: urinary system |
title | Platelet-derived growth factor-D expression in developing and mature human kidneys |
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