Contrasting effects of different levels of food intake and adiposity on LH secretion and hypothalamic gene expression in sheep
Body reserves (long-term) and food intake (short-term) both contribute nutritional feedback to the hypothalamus. Reproductive neuroendocrine output (GnRH/LH) is stimulated by increased food intake and not by high adiposity in sheep, but it is unknown whether appetite-regulating hypothalamic neurons...
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| Veröffentlicht in: | Journal of endocrinology 2002-11, Vol.175 (2), p.383-393 |
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| description | Body reserves (long-term) and food intake (short-term) both contribute nutritional feedback to the hypothalamus. Reproductive neuroendocrine output (GnRH/LH) is stimulated by increased food intake and not by high adiposity in sheep, but it is unknown whether appetite-regulating hypothalamic neurons show this differential response. Castrated male sheep (Scottish Blackface) with oestradiol implants were studied in two 4 week experiments. In Experiment 1, sheep were fed to maintain the initial body condition (BC) score of 2.0+/-0.00 (lower BC (LBC), n=7) or 2.9+/-0.09 (higher BC (HBC), n=9), and liveweight of 43+/-1.1 and 59+/-1.6 kg respectively. LBC and HBC sheep had similar mean plasma LH concentration, pulse frequency and amplitude, but HBC animals had higher mean plasma concentrations of insulin (P |
| doi_str_mv | 10.1677/joe.0.1750383 |
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Reproductive neuroendocrine output (GnRH/LH) is stimulated by increased food intake and not by high adiposity in sheep, but it is unknown whether appetite-regulating hypothalamic neurons show this differential response. Castrated male sheep (Scottish Blackface) with oestradiol implants were studied in two 4 week experiments. In Experiment 1, sheep were fed to maintain the initial body condition (BC) score of 2.0+/-0.00 (lower BC (LBC), n=7) or 2.9+/-0.09 (higher BC (HBC), n=9), and liveweight of 43+/-1.1 and 59+/-1.6 kg respectively. LBC and HBC sheep had similar mean plasma LH concentration, pulse frequency and amplitude, but HBC animals had higher mean plasma concentrations of insulin (P<0.01), leptin (P<0.01) and glucose (P<0.01). Gene expression (measured by in situ hybridisation) in the hypothalamic arcuate nucleus (ARC) was higher in LBC than HBC sheep for neuropeptide Y (NPY; 486% of HBC, P<0.01), agouti-related peptide (AGRP; 467%, P<0.05) and leptin receptor (OB-Rb; 141%, P<0.05), but lower for cocaine- and amphetamine-regulated transcript (CART; 92%, P<0.05) and similar between groups for pro-opiomelanocortin (POMC). In Experiment 2, sheep with initial mean BC score 2.4+/-0.03 and liveweight 55+/-0.8 kg were fed a liveweight-maintenance ration (low intake, LI, n=7) while sheep with initial mean BC score 2.0+/-0.03 and liveweight 43+/-1.4 kg were fed freely so that BC score increased to 2.5+/-0.00 and liveweight increased to 54+/-1.4 kg (high intake, HI, n=9). Compared with LI, HI sheep had higher mean plasma LH (P<0.05), baseline LH (P<0.01) and pulse amplitude (P<0.01) and showed a trend towards higher pulse frequency. Although there were no differences in final mean plasma concentrations, there were significant increases over time in mean concentrations of insulin (P<0.001), leptin (P<0.05) and glucose (P<0.001) in HI sheep. Gene expression for AGRP in the ARC was higher in HI than LI animals (453% of LI; P<0.05), but expression levels were similar for NPY, OB-Rb, CART and POMC. Thus, the hypothalamus shows differential responses to steady-state adiposity as opposed to an increase in food intake, in terms of both reproductive neuroendocrine activity and hypothalamic appetite-regulating pathways. Differences in hypothalamic gene expression were largely consistent with contemporary levels of systemic leptin and insulin feedback; however, increased nutritional feedback was stimulatory to GnRH/LH whereas constant high feedback was not. The hypothalamus therefore has the ability to retain a nutritional memory that can influence subsequent responses.]]></description><identifier>ISSN: 0022-0795</identifier><identifier>EISSN: 1479-6805</identifier><identifier>DOI: 10.1677/joe.0.1750383</identifier><identifier>PMID: 12429036</identifier><identifier>CODEN: JOENAK</identifier><language>eng</language><publisher>Colchester: BioScientifica</publisher><subject>Adipose Tissue - physiology ; Animals ; Autoradiography ; Biological and medical sciences ; Blood Glucose - analysis ; Body Weight ; Eating - physiology ; Fatty Acids, Nonesterified - blood ; Feeding. Feeding behavior ; Fundamental and applied biological sciences. Psychology ; Gene Expression - genetics ; Hypothalamus - physiology ; Insulin - blood ; Leptin - blood ; Luteinizing Hormone - metabolism ; Male ; Orchiectomy ; Sheep - genetics ; Sheep - physiology ; Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><ispartof>Journal of endocrinology, 2002-11, Vol.175 (2), p.383-393</ispartof><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b449t-467dec4d3f72e355be1daac9cd9c7fcc432b313c64c844f9392a1cd99404acdf3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27931,27932</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14024442$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12429036$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Archer, ZA</creatorcontrib><creatorcontrib>Rhind, SM</creatorcontrib><creatorcontrib>Findlay, PA</creatorcontrib><creatorcontrib>Kyle, CE</creatorcontrib><creatorcontrib>Thomas, L</creatorcontrib><creatorcontrib>Marie, M</creatorcontrib><creatorcontrib>Adam, CL</creatorcontrib><title>Contrasting effects of different levels of food intake and adiposity on LH secretion and hypothalamic gene expression in sheep</title><title>Journal of endocrinology</title><addtitle>J Endocrinol</addtitle><description><![CDATA[Body reserves (long-term) and food intake (short-term) both contribute nutritional feedback to the hypothalamus. Reproductive neuroendocrine output (GnRH/LH) is stimulated by increased food intake and not by high adiposity in sheep, but it is unknown whether appetite-regulating hypothalamic neurons show this differential response. Castrated male sheep (Scottish Blackface) with oestradiol implants were studied in two 4 week experiments. In Experiment 1, sheep were fed to maintain the initial body condition (BC) score of 2.0+/-0.00 (lower BC (LBC), n=7) or 2.9+/-0.09 (higher BC (HBC), n=9), and liveweight of 43+/-1.1 and 59+/-1.6 kg respectively. LBC and HBC sheep had similar mean plasma LH concentration, pulse frequency and amplitude, but HBC animals had higher mean plasma concentrations of insulin (P<0.01), leptin (P<0.01) and glucose (P<0.01). Gene expression (measured by in situ hybridisation) in the hypothalamic arcuate nucleus (ARC) was higher in LBC than HBC sheep for neuropeptide Y (NPY; 486% of HBC, P<0.01), agouti-related peptide (AGRP; 467%, P<0.05) and leptin receptor (OB-Rb; 141%, P<0.05), but lower for cocaine- and amphetamine-regulated transcript (CART; 92%, P<0.05) and similar between groups for pro-opiomelanocortin (POMC). In Experiment 2, sheep with initial mean BC score 2.4+/-0.03 and liveweight 55+/-0.8 kg were fed a liveweight-maintenance ration (low intake, LI, n=7) while sheep with initial mean BC score 2.0+/-0.03 and liveweight 43+/-1.4 kg were fed freely so that BC score increased to 2.5+/-0.00 and liveweight increased to 54+/-1.4 kg (high intake, HI, n=9). Compared with LI, HI sheep had higher mean plasma LH (P<0.05), baseline LH (P<0.01) and pulse amplitude (P<0.01) and showed a trend towards higher pulse frequency. Although there were no differences in final mean plasma concentrations, there were significant increases over time in mean concentrations of insulin (P<0.001), leptin (P<0.05) and glucose (P<0.001) in HI sheep. Gene expression for AGRP in the ARC was higher in HI than LI animals (453% of LI; P<0.05), but expression levels were similar for NPY, OB-Rb, CART and POMC. Thus, the hypothalamus shows differential responses to steady-state adiposity as opposed to an increase in food intake, in terms of both reproductive neuroendocrine activity and hypothalamic appetite-regulating pathways. Differences in hypothalamic gene expression were largely consistent with contemporary levels of systemic leptin and insulin feedback; however, increased nutritional feedback was stimulatory to GnRH/LH whereas constant high feedback was not. The hypothalamus therefore has the ability to retain a nutritional memory that can influence subsequent responses.]]></description><subject>Adipose Tissue - physiology</subject><subject>Animals</subject><subject>Autoradiography</subject><subject>Biological and medical sciences</subject><subject>Blood Glucose - analysis</subject><subject>Body Weight</subject><subject>Eating - physiology</subject><subject>Fatty Acids, Nonesterified - blood</subject><subject>Feeding. Feeding behavior</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression - genetics</subject><subject>Hypothalamus - physiology</subject><subject>Insulin - blood</subject><subject>Leptin - blood</subject><subject>Luteinizing Hormone - metabolism</subject><subject>Male</subject><subject>Orchiectomy</subject><subject>Sheep - genetics</subject><subject>Sheep - physiology</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><issn>0022-0795</issn><issn>1479-6805</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90c-P1CAUB3BiNO64evRquOitK79aytFM1DWZxIueGwqPKWsHKjCuc_Fvl7GTbGKiJx7w4UG-IPSSkhvaSfn2LsJNLWVLeM8foQ0VUjVdT9rHaEMIYw2Rqr1Cz3K-I4S2VPKn6IoywRTh3Qb92sZQks7Fhz0G58CUjKPD1tc6QSh4hh8w_1lzMVrsQ9HfAOtgsbZ-idmXE44B725xBpOg-Do5706nJZZJz_rgDd5DAAw_lwQ5n4EPOE8Ay3P0xOk5w4vLeI2-fnj_ZXvb7D5__LR9t2tGIVRpRCctGGG5kwx4245ArdZGGauMdMYIzkZOuemE6YVwiiumad1UgghtrOPX6M3ad0nx-xFyGQ4-G5hnHSAe8yBZ1_O-7ypsVmhSzDmBG5bkDzqdBkqGc-BDDXyo5Rp49a8ujY_jAeyDviRcwesL0Nno2SUdjM8PThAmhGDVidVNfj_d-wTD6GM2vn6Bd97of97P12N_6f-_-jdy-q5E</recordid><startdate>20021101</startdate><enddate>20021101</enddate><creator>Archer, ZA</creator><creator>Rhind, SM</creator><creator>Findlay, PA</creator><creator>Kyle, CE</creator><creator>Thomas, L</creator><creator>Marie, M</creator><creator>Adam, CL</creator><general>BioScientifica</general><general>Portland Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20021101</creationdate><title>Contrasting effects of different levels of food intake and adiposity on LH secretion and hypothalamic gene expression in sheep</title><author>Archer, ZA ; Rhind, SM ; Findlay, PA ; Kyle, CE ; Thomas, L ; Marie, M ; Adam, CL</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b449t-467dec4d3f72e355be1daac9cd9c7fcc432b313c64c844f9392a1cd99404acdf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adipose Tissue - physiology</topic><topic>Animals</topic><topic>Autoradiography</topic><topic>Biological and medical sciences</topic><topic>Blood Glucose - analysis</topic><topic>Body Weight</topic><topic>Eating - physiology</topic><topic>Fatty Acids, Nonesterified - blood</topic><topic>Feeding. Feeding behavior</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression - genetics</topic><topic>Hypothalamus - physiology</topic><topic>Insulin - blood</topic><topic>Leptin - blood</topic><topic>Luteinizing Hormone - metabolism</topic><topic>Male</topic><topic>Orchiectomy</topic><topic>Sheep - genetics</topic><topic>Sheep - physiology</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Archer, ZA</creatorcontrib><creatorcontrib>Rhind, SM</creatorcontrib><creatorcontrib>Findlay, PA</creatorcontrib><creatorcontrib>Kyle, CE</creatorcontrib><creatorcontrib>Thomas, L</creatorcontrib><creatorcontrib>Marie, M</creatorcontrib><creatorcontrib>Adam, CL</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Archer, ZA</au><au>Rhind, SM</au><au>Findlay, PA</au><au>Kyle, CE</au><au>Thomas, L</au><au>Marie, M</au><au>Adam, CL</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Contrasting effects of different levels of food intake and adiposity on LH secretion and hypothalamic gene expression in sheep</atitle><jtitle>Journal of endocrinology</jtitle><addtitle>J Endocrinol</addtitle><date>2002-11-01</date><risdate>2002</risdate><volume>175</volume><issue>2</issue><spage>383</spage><epage>393</epage><pages>383-393</pages><issn>0022-0795</issn><eissn>1479-6805</eissn><coden>JOENAK</coden><abstract><![CDATA[Body reserves (long-term) and food intake (short-term) both contribute nutritional feedback to the hypothalamus. Reproductive neuroendocrine output (GnRH/LH) is stimulated by increased food intake and not by high adiposity in sheep, but it is unknown whether appetite-regulating hypothalamic neurons show this differential response. Castrated male sheep (Scottish Blackface) with oestradiol implants were studied in two 4 week experiments. In Experiment 1, sheep were fed to maintain the initial body condition (BC) score of 2.0+/-0.00 (lower BC (LBC), n=7) or 2.9+/-0.09 (higher BC (HBC), n=9), and liveweight of 43+/-1.1 and 59+/-1.6 kg respectively. LBC and HBC sheep had similar mean plasma LH concentration, pulse frequency and amplitude, but HBC animals had higher mean plasma concentrations of insulin (P<0.01), leptin (P<0.01) and glucose (P<0.01). Gene expression (measured by in situ hybridisation) in the hypothalamic arcuate nucleus (ARC) was higher in LBC than HBC sheep for neuropeptide Y (NPY; 486% of HBC, P<0.01), agouti-related peptide (AGRP; 467%, P<0.05) and leptin receptor (OB-Rb; 141%, P<0.05), but lower for cocaine- and amphetamine-regulated transcript (CART; 92%, P<0.05) and similar between groups for pro-opiomelanocortin (POMC). In Experiment 2, sheep with initial mean BC score 2.4+/-0.03 and liveweight 55+/-0.8 kg were fed a liveweight-maintenance ration (low intake, LI, n=7) while sheep with initial mean BC score 2.0+/-0.03 and liveweight 43+/-1.4 kg were fed freely so that BC score increased to 2.5+/-0.00 and liveweight increased to 54+/-1.4 kg (high intake, HI, n=9). Compared with LI, HI sheep had higher mean plasma LH (P<0.05), baseline LH (P<0.01) and pulse amplitude (P<0.01) and showed a trend towards higher pulse frequency. Although there were no differences in final mean plasma concentrations, there were significant increases over time in mean concentrations of insulin (P<0.001), leptin (P<0.05) and glucose (P<0.001) in HI sheep. Gene expression for AGRP in the ARC was higher in HI than LI animals (453% of LI; P<0.05), but expression levels were similar for NPY, OB-Rb, CART and POMC. Thus, the hypothalamus shows differential responses to steady-state adiposity as opposed to an increase in food intake, in terms of both reproductive neuroendocrine activity and hypothalamic appetite-regulating pathways. Differences in hypothalamic gene expression were largely consistent with contemporary levels of systemic leptin and insulin feedback; however, increased nutritional feedback was stimulatory to GnRH/LH whereas constant high feedback was not. The hypothalamus therefore has the ability to retain a nutritional memory that can influence subsequent responses.]]></abstract><cop>Colchester</cop><pub>BioScientifica</pub><pmid>12429036</pmid><doi>10.1677/joe.0.1750383</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adipose Tissue - physiology Animals Autoradiography Biological and medical sciences Blood Glucose - analysis Body Weight Eating - physiology Fatty Acids, Nonesterified - blood Feeding. Feeding behavior Fundamental and applied biological sciences. Psychology Gene Expression - genetics Hypothalamus - physiology Insulin - blood Leptin - blood Luteinizing Hormone - metabolism Male Orchiectomy Sheep - genetics Sheep - physiology Vertebrates: anatomy and physiology, studies on body, several organs or systems |
| title | Contrasting effects of different levels of food intake and adiposity on LH secretion and hypothalamic gene expression in sheep |
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