A Versatile and Scalable Two-Step Ion-Exchange Chromatography Process for the Purification of Recombinant Adeno-associated Virus Serotypes-2 and -5
Here we describe the development of a two-step chromatography process based on the use of ion-exchange resins for the purification of recombinant adeno-associated virus (rAAV) serotypes-2 and -5. In vitro and in vivo results demonstrate that this method, which does not require any prepurification st...
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| Veröffentlicht in: | Molecular therapy 2002-11, Vol.6 (5), p.678-686 |
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| creator | Brument, Nicole Morenweiser, Robert Blouin, Véronique Toublanc, Estelle Raimbaud, Isabelle Chérel, Yan Folliot, Sébastien Gaden, Florence Boulanger, Pierre Kroner-Lux, Gabrielle Moullier, Philippe Rolling, Fabienne Salvetti, Anna |
| description | Here we describe the development of a two-step chromatography process based on the use of ion-exchange resins for the purification of recombinant adeno-associated virus (rAAV) serotypes-2 and -5. In vitro and in vivo results demonstrate that this method, which does not require any prepurification step of the cell lysate, can be applied to obtain highly pure rAAV2 and rAAV5 stocks. As such, this procedure can be easily transferred in vector cores and also scaled up, allowing the direct comparison of these two, and potentially other, AAV serotypes in large animal models. |
| doi_str_mv | 10.1006/mthe.2002.0719 |
| format | Article |
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In vitro and in vivo results demonstrate that this method, which does not require any prepurification step of the cell lysate, can be applied to obtain highly pure rAAV2 and rAAV5 stocks. As such, this procedure can be easily transferred in vector cores and also scaled up, allowing the direct comparison of these two, and potentially other, AAV serotypes in large animal models.</description><identifier>ISSN: 1525-0016</identifier><identifier>EISSN: 1525-0024</identifier><identifier>DOI: 10.1006/mthe.2002.0719</identifier><identifier>PMID: 12436964</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>AAV purification ; Adenoviruses ; Animals ; Blotting, Western ; Cell Line ; Cells ; Cesium - pharmacology ; Chlorides - pharmacology ; Chromatography ; Chromatography, Ion Exchange - methods ; Chromatography, Liquid ; Dependovirus - genetics ; Dependovirus - isolation & purification ; Gene therapy ; Genetic Techniques ; HeLa Cells ; Humans ; ion-exchange chromatography ; Life Sciences ; Mice ; Mice, Inbred BALB C ; Microbiology and Parasitology ; Microscopy, Electron ; Plasmids - metabolism ; Rats ; Rats, Wistar ; Resins ; Triiodobenzoic Acids - pharmacology ; Vectors (Biology) ; Virology</subject><ispartof>Molecular therapy, 2002-11, Vol.6 (5), p.678-686</ispartof><rights>2002 American Society for Gene Therapy</rights><rights>Copyright Nature Publishing Group Nov 2002</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3139-3f3d8af309485f2e2c1b54e58ca02d759230091b20887872b8d2ce66046a079e3</citedby><cites>FETCH-LOGICAL-c3139-3f3d8af309485f2e2c1b54e58ca02d759230091b20887872b8d2ce66046a079e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1792815217?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>315,782,786,887,27931,27932,64392,64394,64396,72476</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12436964$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00112428$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Brument, Nicole</creatorcontrib><creatorcontrib>Morenweiser, Robert</creatorcontrib><creatorcontrib>Blouin, Véronique</creatorcontrib><creatorcontrib>Toublanc, Estelle</creatorcontrib><creatorcontrib>Raimbaud, Isabelle</creatorcontrib><creatorcontrib>Chérel, Yan</creatorcontrib><creatorcontrib>Folliot, Sébastien</creatorcontrib><creatorcontrib>Gaden, Florence</creatorcontrib><creatorcontrib>Boulanger, Pierre</creatorcontrib><creatorcontrib>Kroner-Lux, Gabrielle</creatorcontrib><creatorcontrib>Moullier, Philippe</creatorcontrib><creatorcontrib>Rolling, Fabienne</creatorcontrib><creatorcontrib>Salvetti, Anna</creatorcontrib><title>A Versatile and Scalable Two-Step Ion-Exchange Chromatography Process for the Purification of Recombinant Adeno-associated Virus Serotypes-2 and -5</title><title>Molecular therapy</title><addtitle>Mol Ther</addtitle><description>Here we describe the development of a two-step chromatography process based on the use of ion-exchange resins for the purification of recombinant adeno-associated virus (rAAV) serotypes-2 and -5. 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As such, this procedure can be easily transferred in vector cores and also scaled up, allowing the direct comparison of these two, and potentially other, AAV serotypes in large animal models.</description><subject>AAV purification</subject><subject>Adenoviruses</subject><subject>Animals</subject><subject>Blotting, Western</subject><subject>Cell Line</subject><subject>Cells</subject><subject>Cesium - pharmacology</subject><subject>Chlorides - pharmacology</subject><subject>Chromatography</subject><subject>Chromatography, Ion Exchange - methods</subject><subject>Chromatography, Liquid</subject><subject>Dependovirus - genetics</subject><subject>Dependovirus - isolation & purification</subject><subject>Gene therapy</subject><subject>Genetic Techniques</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>ion-exchange chromatography</subject><subject>Life Sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology and Parasitology</subject><subject>Microscopy, Electron</subject><subject>Plasmids - 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pharmacology</topic><topic>Chlorides - pharmacology</topic><topic>Chromatography</topic><topic>Chromatography, Ion Exchange - methods</topic><topic>Chromatography, Liquid</topic><topic>Dependovirus - genetics</topic><topic>Dependovirus - isolation & purification</topic><topic>Gene therapy</topic><topic>Genetic Techniques</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>ion-exchange chromatography</topic><topic>Life Sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology and Parasitology</topic><topic>Microscopy, Electron</topic><topic>Plasmids - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Resins</topic><topic>Triiodobenzoic Acids - pharmacology</topic><topic>Vectors (Biology)</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brument, Nicole</creatorcontrib><creatorcontrib>Morenweiser, Robert</creatorcontrib><creatorcontrib>Blouin, Véronique</creatorcontrib><creatorcontrib>Toublanc, Estelle</creatorcontrib><creatorcontrib>Raimbaud, Isabelle</creatorcontrib><creatorcontrib>Chérel, Yan</creatorcontrib><creatorcontrib>Folliot, Sébastien</creatorcontrib><creatorcontrib>Gaden, Florence</creatorcontrib><creatorcontrib>Boulanger, Pierre</creatorcontrib><creatorcontrib>Kroner-Lux, Gabrielle</creatorcontrib><creatorcontrib>Moullier, Philippe</creatorcontrib><creatorcontrib>Rolling, Fabienne</creatorcontrib><creatorcontrib>Salvetti, Anna</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - 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| ispartof | Molecular therapy, 2002-11, Vol.6 (5), p.678-686 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; ProQuest Central UK/Ireland; Alma/SFX Local Collection |
| subjects | AAV purification Adenoviruses Animals Blotting, Western Cell Line Cells Cesium - pharmacology Chlorides - pharmacology Chromatography Chromatography, Ion Exchange - methods Chromatography, Liquid Dependovirus - genetics Dependovirus - isolation & purification Gene therapy Genetic Techniques HeLa Cells Humans ion-exchange chromatography Life Sciences Mice Mice, Inbred BALB C Microbiology and Parasitology Microscopy, Electron Plasmids - metabolism Rats Rats, Wistar Resins Triiodobenzoic Acids - pharmacology Vectors (Biology) Virology |
| title | A Versatile and Scalable Two-Step Ion-Exchange Chromatography Process for the Purification of Recombinant Adeno-associated Virus Serotypes-2 and -5 |
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