Characterization of the immune response against hepatitis C infection in recovered, and chronically infected chimpanzees
The immune response to hepatitis C virus (HCV) is believed to be critical in determining the outcome of the disease. In this study we have analysed epitope recognition, cytokine profile, and anti‐HCV antibody responses in chronically HCV‐infected and recovered chimpanzees. Quantitative measurement o...
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description | The immune response to hepatitis C virus (HCV) is believed to be critical in determining the outcome of the disease. In this study we have analysed epitope recognition, cytokine profile, and anti‐HCV antibody responses in chronically HCV‐infected and recovered chimpanzees. Quantitative measurement of anti‐HCV antibody in HCV‐infected chimpanzees revealed that the response in HCV‐ recovered chimpanzees peaked within 4–20 weeks. In contrast, the anti‐HCV antibody responses in chronically HCV infected chimpanzees did not peak until 100–200 weeks after infection, and decreased gradually thereafter. T cell proliferation assays measuring responses to pooled HCV proteins revealed significant increases in the 3H‐uptake during the early stages of infection in recovered chimpanzees in comparison to the chronically infected ones. Class I‐restricted epitopes of the core, and NS3 proteins of HCV were analysed using 9–10 mer overlapping peptides covering the core and NS3 proteins, and IFN‐γ ELISPOT technique. Our data indicated early and broad class‐I restricted core, and NS3 protein epitope recognitions in HCV‐recovered chimpanzees but not in chimpanzees that had been chronically infected. Additionally, dominant epitopes recognized early in infection (8 weeks) were no longer recognized later in infection (followed up to 64 weeks). Cytokines profiling revealed a 50‐fold increase in TNF‐α secretion in the supernatant of core‐specific CD8 memory cells of the chronically infected chimpanzees in comparison to the recovered ones. In summary, multiple parameters correlate with HCV recovery in chimpanzees. |
doi_str_mv | 10.1046/j.1365-2893.2002.00373.x |
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T. ; Anthony, D. D. ; Carlson, N. L. ; Andrus, L. ; Brotman, B. ; Tricoche, N. ; McCormack, P. ; Prince, A.</creator><creatorcontrib>Shata, M. T. ; Anthony, D. D. ; Carlson, N. L. ; Andrus, L. ; Brotman, B. ; Tricoche, N. ; McCormack, P. ; Prince, A.</creatorcontrib><description>The immune response to hepatitis C virus (HCV) is believed to be critical in determining the outcome of the disease. In this study we have analysed epitope recognition, cytokine profile, and anti‐HCV antibody responses in chronically HCV‐infected and recovered chimpanzees. Quantitative measurement of anti‐HCV antibody in HCV‐infected chimpanzees revealed that the response in HCV‐ recovered chimpanzees peaked within 4–20 weeks. In contrast, the anti‐HCV antibody responses in chronically HCV infected chimpanzees did not peak until 100–200 weeks after infection, and decreased gradually thereafter. T cell proliferation assays measuring responses to pooled HCV proteins revealed significant increases in the 3H‐uptake during the early stages of infection in recovered chimpanzees in comparison to the chronically infected ones. Class I‐restricted epitopes of the core, and NS3 proteins of HCV were analysed using 9–10 mer overlapping peptides covering the core and NS3 proteins, and IFN‐γ ELISPOT technique. Our data indicated early and broad class‐I restricted core, and NS3 protein epitope recognitions in HCV‐recovered chimpanzees but not in chimpanzees that had been chronically infected. Additionally, dominant epitopes recognized early in infection (8 weeks) were no longer recognized later in infection (followed up to 64 weeks). Cytokines profiling revealed a 50‐fold increase in TNF‐α secretion in the supernatant of core‐specific CD8 memory cells of the chronically infected chimpanzees in comparison to the recovered ones. In summary, multiple parameters correlate with HCV recovery in chimpanzees.</description><identifier>ISSN: 1352-0504</identifier><identifier>EISSN: 1365-2893</identifier><identifier>DOI: 10.1046/j.1365-2893.2002.00373.x</identifier><identifier>PMID: 12431201</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Animals ; CD4-Positive T-Lymphocytes - immunology ; CD8-Positive T-Lymphocytes - immunology ; core peptides ; Disease Models, Animal ; Epitope Mapping ; Epitopes, T-Lymphocyte ; Female ; Hepacivirus - immunology ; hepatitis C ; Hepatitis C - immunology ; Hepatitis C - virology ; Hepatitis C Antibodies - blood ; Hepatitis C, Chronic - immunology ; Hepatitis C, Chronic - virology ; Histocompatibility Antigens Class I - metabolism ; Humans ; immune response ; Male ; NS3 peptides ; Pan troglodytes ; Peptides - immunology ; TNF-α ; Tumor Necrosis Factor-alpha - metabolism ; Viral Core Proteins - chemistry ; Viral Core Proteins - immunology ; Viral Nonstructural Proteins - immunology</subject><ispartof>Journal of viral hepatitis, 2002-11, Vol.9 (6), p.400-410</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4333-f11390c024e2289ecc4b27604c84d2b33162a620c250e2d032ddb88867eb70723</citedby><cites>FETCH-LOGICAL-c4333-f11390c024e2289ecc4b27604c84d2b33162a620c250e2d032ddb88867eb70723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2893.2002.00373.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2893.2002.00373.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12431201$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shata, M. T.</creatorcontrib><creatorcontrib>Anthony, D. D.</creatorcontrib><creatorcontrib>Carlson, N. L.</creatorcontrib><creatorcontrib>Andrus, L.</creatorcontrib><creatorcontrib>Brotman, B.</creatorcontrib><creatorcontrib>Tricoche, N.</creatorcontrib><creatorcontrib>McCormack, P.</creatorcontrib><creatorcontrib>Prince, A.</creatorcontrib><title>Characterization of the immune response against hepatitis C infection in recovered, and chronically infected chimpanzees</title><title>Journal of viral hepatitis</title><addtitle>J Viral Hepat</addtitle><description>The immune response to hepatitis C virus (HCV) is believed to be critical in determining the outcome of the disease. In this study we have analysed epitope recognition, cytokine profile, and anti‐HCV antibody responses in chronically HCV‐infected and recovered chimpanzees. Quantitative measurement of anti‐HCV antibody in HCV‐infected chimpanzees revealed that the response in HCV‐ recovered chimpanzees peaked within 4–20 weeks. In contrast, the anti‐HCV antibody responses in chronically HCV infected chimpanzees did not peak until 100–200 weeks after infection, and decreased gradually thereafter. T cell proliferation assays measuring responses to pooled HCV proteins revealed significant increases in the 3H‐uptake during the early stages of infection in recovered chimpanzees in comparison to the chronically infected ones. Class I‐restricted epitopes of the core, and NS3 proteins of HCV were analysed using 9–10 mer overlapping peptides covering the core and NS3 proteins, and IFN‐γ ELISPOT technique. Our data indicated early and broad class‐I restricted core, and NS3 protein epitope recognitions in HCV‐recovered chimpanzees but not in chimpanzees that had been chronically infected. Additionally, dominant epitopes recognized early in infection (8 weeks) were no longer recognized later in infection (followed up to 64 weeks). Cytokines profiling revealed a 50‐fold increase in TNF‐α secretion in the supernatant of core‐specific CD8 memory cells of the chronically infected chimpanzees in comparison to the recovered ones. In summary, multiple parameters correlate with HCV recovery in chimpanzees.</description><subject>Animals</subject><subject>CD4-Positive T-Lymphocytes - immunology</subject><subject>CD8-Positive T-Lymphocytes - immunology</subject><subject>core peptides</subject><subject>Disease Models, Animal</subject><subject>Epitope Mapping</subject><subject>Epitopes, T-Lymphocyte</subject><subject>Female</subject><subject>Hepacivirus - immunology</subject><subject>hepatitis C</subject><subject>Hepatitis C - immunology</subject><subject>Hepatitis C - virology</subject><subject>Hepatitis C Antibodies - blood</subject><subject>Hepatitis C, Chronic - immunology</subject><subject>Hepatitis C, Chronic - virology</subject><subject>Histocompatibility Antigens Class I - metabolism</subject><subject>Humans</subject><subject>immune response</subject><subject>Male</subject><subject>NS3 peptides</subject><subject>Pan troglodytes</subject><subject>Peptides - immunology</subject><subject>TNF-α</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><subject>Viral Core Proteins - chemistry</subject><subject>Viral Core Proteins - immunology</subject><subject>Viral Nonstructural Proteins - immunology</subject><issn>1352-0504</issn><issn>1365-2893</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU2P0zAQhiMEYpeFv4B84kTCeJzEqcQFVbAfKiDxsStxsRxnQl0SJ9gptPvrcbbVcoSTR57nHXv0JAnjkHHIy1ebjIuySLFaiAwBMAMQUmS7B8npfePhXBeYQgH5SfIkhA0AF1jwx8kJx1xwBH6a7JZr7bWZyNtbPdnBsaFl05qY7futI-YpjIMLxPR3bV2Y2JrGyE02sCWzriVzF7Iukmb4RZ6al0y7hpm1H5w1uuv2R47mS9uP2t0ShafJo1Z3gZ4dz7Pk67u3X5YX6erj-eXyzSo1uRAibTkXCzCAOWHciozJa5Ql5KbKG6yF4CXqEsFgAYQNCGyauqqqUlItQaI4S14c5o5--LmlMKneBkNdpx0N26AklnKBi_yfIK9KlEUpI1gdQOOHEDy1avS2136vOKhZj9qo2YKaLahZj7rTo3Yx-vz4xrbuqfkbPPqIwOsD8Nt2tP_vwerq-iIWMZ4e4jZMtLuPa_9DxY_LQt18OFefrt-vxLfVZ3Uj_gAYNq5F</recordid><startdate>200211</startdate><enddate>200211</enddate><creator>Shata, M. T.</creator><creator>Anthony, D. D.</creator><creator>Carlson, N. L.</creator><creator>Andrus, L.</creator><creator>Brotman, B.</creator><creator>Tricoche, N.</creator><creator>McCormack, P.</creator><creator>Prince, A.</creator><general>Blackwell Science Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200211</creationdate><title>Characterization of the immune response against hepatitis C infection in recovered, and chronically infected chimpanzees</title><author>Shata, M. T. ; Anthony, D. D. ; Carlson, N. L. ; Andrus, L. ; Brotman, B. ; Tricoche, N. ; McCormack, P. ; Prince, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4333-f11390c024e2289ecc4b27604c84d2b33162a620c250e2d032ddb88867eb70723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>CD4-Positive T-Lymphocytes - immunology</topic><topic>CD8-Positive T-Lymphocytes - immunology</topic><topic>core peptides</topic><topic>Disease Models, Animal</topic><topic>Epitope Mapping</topic><topic>Epitopes, T-Lymphocyte</topic><topic>Female</topic><topic>Hepacivirus - immunology</topic><topic>hepatitis C</topic><topic>Hepatitis C - immunology</topic><topic>Hepatitis C - virology</topic><topic>Hepatitis C Antibodies - blood</topic><topic>Hepatitis C, Chronic - immunology</topic><topic>Hepatitis C, Chronic - virology</topic><topic>Histocompatibility Antigens Class I - metabolism</topic><topic>Humans</topic><topic>immune response</topic><topic>Male</topic><topic>NS3 peptides</topic><topic>Pan troglodytes</topic><topic>Peptides - immunology</topic><topic>TNF-α</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><topic>Viral Core Proteins - chemistry</topic><topic>Viral Core Proteins - immunology</topic><topic>Viral Nonstructural Proteins - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shata, M. T.</creatorcontrib><creatorcontrib>Anthony, D. D.</creatorcontrib><creatorcontrib>Carlson, N. L.</creatorcontrib><creatorcontrib>Andrus, L.</creatorcontrib><creatorcontrib>Brotman, B.</creatorcontrib><creatorcontrib>Tricoche, N.</creatorcontrib><creatorcontrib>McCormack, P.</creatorcontrib><creatorcontrib>Prince, A.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of viral hepatitis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shata, M. T.</au><au>Anthony, D. D.</au><au>Carlson, N. L.</au><au>Andrus, L.</au><au>Brotman, B.</au><au>Tricoche, N.</au><au>McCormack, P.</au><au>Prince, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of the immune response against hepatitis C infection in recovered, and chronically infected chimpanzees</atitle><jtitle>Journal of viral hepatitis</jtitle><addtitle>J Viral Hepat</addtitle><date>2002-11</date><risdate>2002</risdate><volume>9</volume><issue>6</issue><spage>400</spage><epage>410</epage><pages>400-410</pages><issn>1352-0504</issn><eissn>1365-2893</eissn><abstract>The immune response to hepatitis C virus (HCV) is believed to be critical in determining the outcome of the disease. In this study we have analysed epitope recognition, cytokine profile, and anti‐HCV antibody responses in chronically HCV‐infected and recovered chimpanzees. Quantitative measurement of anti‐HCV antibody in HCV‐infected chimpanzees revealed that the response in HCV‐ recovered chimpanzees peaked within 4–20 weeks. In contrast, the anti‐HCV antibody responses in chronically HCV infected chimpanzees did not peak until 100–200 weeks after infection, and decreased gradually thereafter. T cell proliferation assays measuring responses to pooled HCV proteins revealed significant increases in the 3H‐uptake during the early stages of infection in recovered chimpanzees in comparison to the chronically infected ones. Class I‐restricted epitopes of the core, and NS3 proteins of HCV were analysed using 9–10 mer overlapping peptides covering the core and NS3 proteins, and IFN‐γ ELISPOT technique. Our data indicated early and broad class‐I restricted core, and NS3 protein epitope recognitions in HCV‐recovered chimpanzees but not in chimpanzees that had been chronically infected. Additionally, dominant epitopes recognized early in infection (8 weeks) were no longer recognized later in infection (followed up to 64 weeks). Cytokines profiling revealed a 50‐fold increase in TNF‐α secretion in the supernatant of core‐specific CD8 memory cells of the chronically infected chimpanzees in comparison to the recovered ones. In summary, multiple parameters correlate with HCV recovery in chimpanzees.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>12431201</pmid><doi>10.1046/j.1365-2893.2002.00373.x</doi><tpages>11</tpages></addata></record> |
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subjects | Animals CD4-Positive T-Lymphocytes - immunology CD8-Positive T-Lymphocytes - immunology core peptides Disease Models, Animal Epitope Mapping Epitopes, T-Lymphocyte Female Hepacivirus - immunology hepatitis C Hepatitis C - immunology Hepatitis C - virology Hepatitis C Antibodies - blood Hepatitis C, Chronic - immunology Hepatitis C, Chronic - virology Histocompatibility Antigens Class I - metabolism Humans immune response Male NS3 peptides Pan troglodytes Peptides - immunology TNF-α Tumor Necrosis Factor-alpha - metabolism Viral Core Proteins - chemistry Viral Core Proteins - immunology Viral Nonstructural Proteins - immunology |
title | Characterization of the immune response against hepatitis C infection in recovered, and chronically infected chimpanzees |
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