Insect cells as a factory to produce adeno-associated virus type 2 vectors

Recombinant adeno-associated viruses (rAAV) are produced transiently in mammalian cells usually by cotransfecting two or three plasmids containing AAV genes, adenovirus helper genes, and a vector genome. Expansion and transfection of adherent cells limit the scale of rAAV production. Efficient trans...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Human gene therapy 2002-11, Vol.13 (16), p.1935-1943
Hauptverfasser:	URABE, Masashi, CHUANTIAN DING, KOTIN, Robert M
Format:	Artikel
Sprache:	eng
Schlagworte:	Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Animals Applied cell therapy and gene therapy Biological and medical sciences Biotechnology Blotting, Southern Blotting, Western Cell Adhesion Cell Line Centrifugation, Density Gradient Cesium - pharmacology Chlorides - pharmacology Dependovirus - genetics Fundamental and applied biological sciences. Psychology Gene therapy Gene Transfer Techniques Genetic Vectors Green Fluorescent Proteins Health. Pharmaceutical industry Humans Industrial applications and implications. Economical aspects Insecta Luminescent Proteins - metabolism Medical sciences Mice Models, Genetic Plasmids - metabolism Retina - metabolism Transcription, Genetic Transfection Transfusions. Complications. Transfusion reactions. Cell and gene therapy
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!