Purification and crystallization of N-terminally truncated forms of microsomal cytochrome P450 2C5

This chapter describes the preparation and crystallization of the enzyme and discusses procedures contributing to the improved resolution of the crystals that should be applicable to other modified membrane P450s. Engineering more soluble forms of P450 2C5 has contributed to the crystallization of t...

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Veröffentlicht in:	Methods in Enzymology 2002, Vol.357, p.73-79
Hauptverfasser:	Wester, Michael R., David Stout, C., Johnson, Eric E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals BASIC BIOLOGICAL SCIENCES Cryoprotective Agents - chemistry CRYSTALLIZATION Cytochrome P-450 Enzyme System - chemistry Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - isolation & purification Cytochrome P450 Family 2 CYTOCHROMES Detergents - chemistry Microsomes - enzymology PURIFICATION Rabbits STANFORD SYNCHROTRON RADIATION LABORATORY Steroid 21-Hydroxylase - chemistry Steroid 21-Hydroxylase - genetics Steroid 21-Hydroxylase - isolation & purification X-Ray Diffraction
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