A possible pitfall in the identification of Burkholderia mallei using molecular identification systems based on the sequence of the flagellin fliC gene
Abstract Amotile Burkholderia mallei and motile Burkholderia pseudomallei display a high similarity with regard to phenotype and clinical syndromes, glanders and melioidosis. The aim of this study was to establish a fast and reliable molecular method for identification and differentiation. Despite a...
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| Veröffentlicht in: | FEMS immunology and medical microbiology 2002-11, Vol.34 (3), p.231-236 |
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| creator | Sprague, Lisa D. Zysk, Gregor Hagen, Ralf M. Meyer, Hermann Ellis, Jill Anuntagool, Narisara Gauthier, Yves Neubauer, Heinrich |
| description | Abstract
Amotile Burkholderia mallei and motile Burkholderia pseudomallei display a high similarity with regard to phenotype and clinical syndromes, glanders and melioidosis. The aim of this study was to establish a fast and reliable molecular method for identification and differentiation. Despite amotility, the gene of the filament forming flagellin (fliC) could be completely sequenced in two B. mallei strains. Only one mutation was identified discriminating between B. mallei and B. pseudomallei. A polymerase chain reaction-restriction fragment length polymorphism assay was designed making use of the absence of an Ava II recognition site in B. mallei. All seven B. mallei, 12 out of 15 B. pseudomallei and 36 closely related apathogenic Burkholderia thailandensis strains were identified correctly. However, in three B. pseudomallei strains a point mutation at gene position 798 (G to C) disrupted the AvaII site. Therefore, molecular systems based on the fliC sequence can be used for a reliable proof of strains of the three species but not for the differentiation of B. mallei and B. pseudomallei isolates. |
| doi_str_mv | 10.1111/j.1574-695X.2002.tb00629.x |
| format | Article |
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Amotile Burkholderia mallei and motile Burkholderia pseudomallei display a high similarity with regard to phenotype and clinical syndromes, glanders and melioidosis. The aim of this study was to establish a fast and reliable molecular method for identification and differentiation. Despite amotility, the gene of the filament forming flagellin (fliC) could be completely sequenced in two B. mallei strains. Only one mutation was identified discriminating between B. mallei and B. pseudomallei. A polymerase chain reaction-restriction fragment length polymorphism assay was designed making use of the absence of an Ava II recognition site in B. mallei. All seven B. mallei, 12 out of 15 B. pseudomallei and 36 closely related apathogenic Burkholderia thailandensis strains were identified correctly. However, in three B. pseudomallei strains a point mutation at gene position 798 (G to C) disrupted the AvaII site. Therefore, molecular systems based on the fliC sequence can be used for a reliable proof of strains of the three species but not for the differentiation of B. mallei and B. pseudomallei isolates.</description><identifier>ISSN: 0928-8244</identifier><identifier>EISSN: 1574-695X</identifier><identifier>EISSN: 2049-632X</identifier><identifier>DOI: 10.1111/j.1574-695X.2002.tb00629.x</identifier><identifier>PMID: 12423776</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Burkholderia - classification ; Burkholderia - isolation & purification ; Burkholderia mallei ; Burkholderia pseudomallei ; Burkholderia thailandensis ; Differentiation ; Flagellin ; Flagellin - genetics ; Flagellin - isolation & purification ; fliC ; Fundamental and applied biological sciences. Psychology ; Genes, Bacterial ; Glanders ; Melioidosis ; Microbiology ; Molecular Sequence Data ; Mutation ; Phenotypes ; Point mutation ; Polymerase chain reaction ; Polymerase Chain Reaction - methods ; Polymorphism ; Polymorphism, Restriction Fragment Length ; Restriction fragment length polymorphism ; Sensitivity and Specificity ; Sequence Alignment</subject><ispartof>FEMS immunology and medical microbiology, 2002-11, Vol.34 (3), p.231-236</ispartof><rights>2002 Federation of European Microbiological Societies. 2002</rights><rights>2003 INIST-CNRS</rights><rights>2002 Federation of European Microbiological Societies.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4371-1132ccf5d17f5831cf6291680ff556ff04e796d3689d14e140ea8e7bf0fa0a513</citedby><cites>FETCH-LOGICAL-c4371-1132ccf5d17f5831cf6291680ff556ff04e796d3689d14e140ea8e7bf0fa0a513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-695X.2002.tb00629.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-695X.2002.tb00629.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14800638$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12423776$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sprague, Lisa D.</creatorcontrib><creatorcontrib>Zysk, Gregor</creatorcontrib><creatorcontrib>Hagen, Ralf M.</creatorcontrib><creatorcontrib>Meyer, Hermann</creatorcontrib><creatorcontrib>Ellis, Jill</creatorcontrib><creatorcontrib>Anuntagool, Narisara</creatorcontrib><creatorcontrib>Gauthier, Yves</creatorcontrib><creatorcontrib>Neubauer, Heinrich</creatorcontrib><title>A possible pitfall in the identification of Burkholderia mallei using molecular identification systems based on the sequence of the flagellin fliC gene</title><title>FEMS immunology and medical microbiology</title><addtitle>FEMS Immunol Med Microbiol</addtitle><description>Abstract
Amotile Burkholderia mallei and motile Burkholderia pseudomallei display a high similarity with regard to phenotype and clinical syndromes, glanders and melioidosis. The aim of this study was to establish a fast and reliable molecular method for identification and differentiation. Despite amotility, the gene of the filament forming flagellin (fliC) could be completely sequenced in two B. mallei strains. Only one mutation was identified discriminating between B. mallei and B. pseudomallei. A polymerase chain reaction-restriction fragment length polymorphism assay was designed making use of the absence of an Ava II recognition site in B. mallei. All seven B. mallei, 12 out of 15 B. pseudomallei and 36 closely related apathogenic Burkholderia thailandensis strains were identified correctly. However, in three B. pseudomallei strains a point mutation at gene position 798 (G to C) disrupted the AvaII site. Therefore, molecular systems based on the fliC sequence can be used for a reliable proof of strains of the three species but not for the differentiation of B. mallei and B. pseudomallei isolates.</description><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Burkholderia - classification</subject><subject>Burkholderia - isolation & purification</subject><subject>Burkholderia mallei</subject><subject>Burkholderia pseudomallei</subject><subject>Burkholderia thailandensis</subject><subject>Differentiation</subject><subject>Flagellin</subject><subject>Flagellin - genetics</subject><subject>Flagellin - isolation & purification</subject><subject>fliC</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>Glanders</subject><subject>Melioidosis</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Phenotypes</subject><subject>Point mutation</subject><subject>Polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymorphism</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Restriction fragment length polymorphism</subject><subject>Sensitivity and Specificity</subject><subject>Sequence Alignment</subject><issn>0928-8244</issn><issn>1574-695X</issn><issn>2049-632X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkdGK1TAQhoMo7tnVV5Cg6F3rJE3T1gthPbi6sOKNgnchTSdnc0zbY9PinifZ1zWlxYVFEXMTQr7555_5CXnOIGXxvN6nLC9EIqv8W8oBeDrWAJJX6c0Dsvn99ZBsoOJlUnIhTshpCHsAEBXAY3LCuOBZUcgNuT2nhz4EV3ukBzda7T11HR2vkboGu9FZZ_To-o72lr6bhu_XvW9wcJq2EUVHp-C6HW17j2byerhfFY5hxDbQWgdsaL8oB_wxYWdw1pzf1usdeh_7Wu-2dIcdPiGPopeAT9f7jHy9eP9l-zG5-vzhcnt-lRiRFSxhLOPG2Lxhhc3LjBkb98BkCdbmubQWBBaVbDJZVg0TyASgLrGoLVgNOmfZGXm16B6GPpoKo2pdMNGM7rCfgiq4LHKQ8p8gKyUTZZZF8MU9cN9PQxeHUDxjDCQUHCL1ZqHMENc_oFWHwbV6OCoGak5Z7dUcpZqjVHPKak1Z3cTiZ2uLqW6xuStdY43AyxXQwWhvB90ZF-44UUaprIzc24X76Twe_8OCurj8FKeJAvki0E-Hv5Qnf5rgF4vI1j4</recordid><startdate>20021115</startdate><enddate>20021115</enddate><creator>Sprague, Lisa D.</creator><creator>Zysk, Gregor</creator><creator>Hagen, Ralf M.</creator><creator>Meyer, Hermann</creator><creator>Ellis, Jill</creator><creator>Anuntagool, Narisara</creator><creator>Gauthier, Yves</creator><creator>Neubauer, Heinrich</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7QL</scope><scope>7X8</scope></search><sort><creationdate>20021115</creationdate><title>A possible pitfall in the identification of Burkholderia mallei using molecular identification systems based on the sequence of the flagellin fliC gene</title><author>Sprague, Lisa D. ; Zysk, Gregor ; Hagen, Ralf M. ; Meyer, Hermann ; Ellis, Jill ; Anuntagool, Narisara ; Gauthier, Yves ; Neubauer, Heinrich</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4371-1132ccf5d17f5831cf6291680ff556ff04e796d3689d14e140ea8e7bf0fa0a513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Burkholderia - classification</topic><topic>Burkholderia - isolation & purification</topic><topic>Burkholderia mallei</topic><topic>Burkholderia pseudomallei</topic><topic>Burkholderia thailandensis</topic><topic>Differentiation</topic><topic>Flagellin</topic><topic>Flagellin - genetics</topic><topic>Flagellin - isolation & purification</topic><topic>fliC</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial</topic><topic>Glanders</topic><topic>Melioidosis</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Phenotypes</topic><topic>Point mutation</topic><topic>Polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Polymorphism</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Restriction fragment length polymorphism</topic><topic>Sensitivity and Specificity</topic><topic>Sequence Alignment</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sprague, Lisa D.</creatorcontrib><creatorcontrib>Zysk, Gregor</creatorcontrib><creatorcontrib>Hagen, Ralf M.</creatorcontrib><creatorcontrib>Meyer, Hermann</creatorcontrib><creatorcontrib>Ellis, Jill</creatorcontrib><creatorcontrib>Anuntagool, Narisara</creatorcontrib><creatorcontrib>Gauthier, Yves</creatorcontrib><creatorcontrib>Neubauer, Heinrich</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS immunology and medical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sprague, Lisa D.</au><au>Zysk, Gregor</au><au>Hagen, Ralf M.</au><au>Meyer, Hermann</au><au>Ellis, Jill</au><au>Anuntagool, Narisara</au><au>Gauthier, Yves</au><au>Neubauer, Heinrich</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A possible pitfall in the identification of Burkholderia mallei using molecular identification systems based on the sequence of the flagellin fliC gene</atitle><jtitle>FEMS immunology and medical microbiology</jtitle><addtitle>FEMS Immunol Med Microbiol</addtitle><date>2002-11-15</date><risdate>2002</risdate><volume>34</volume><issue>3</issue><spage>231</spage><epage>236</epage><pages>231-236</pages><issn>0928-8244</issn><eissn>1574-695X</eissn><eissn>2049-632X</eissn><abstract>Abstract
Amotile Burkholderia mallei and motile Burkholderia pseudomallei display a high similarity with regard to phenotype and clinical syndromes, glanders and melioidosis. The aim of this study was to establish a fast and reliable molecular method for identification and differentiation. Despite amotility, the gene of the filament forming flagellin (fliC) could be completely sequenced in two B. mallei strains. Only one mutation was identified discriminating between B. mallei and B. pseudomallei. A polymerase chain reaction-restriction fragment length polymorphism assay was designed making use of the absence of an Ava II recognition site in B. mallei. All seven B. mallei, 12 out of 15 B. pseudomallei and 36 closely related apathogenic Burkholderia thailandensis strains were identified correctly. However, in three B. pseudomallei strains a point mutation at gene position 798 (G to C) disrupted the AvaII site. Therefore, molecular systems based on the fliC sequence can be used for a reliable proof of strains of the three species but not for the differentiation of B. mallei and B. pseudomallei isolates.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>12423776</pmid><doi>10.1111/j.1574-695X.2002.tb00629.x</doi><tpages>6</tpages></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Bacteriological methods and techniques used in bacteriology Bacteriology Base Sequence Biological and medical sciences Burkholderia - classification Burkholderia - isolation & purification Burkholderia mallei Burkholderia pseudomallei Burkholderia thailandensis Differentiation Flagellin Flagellin - genetics Flagellin - isolation & purification fliC Fundamental and applied biological sciences. Psychology Genes, Bacterial Glanders Melioidosis Microbiology Molecular Sequence Data Mutation Phenotypes Point mutation Polymerase chain reaction Polymerase Chain Reaction - methods Polymorphism Polymorphism, Restriction Fragment Length Restriction fragment length polymorphism Sensitivity and Specificity Sequence Alignment |
| title | A possible pitfall in the identification of Burkholderia mallei using molecular identification systems based on the sequence of the flagellin fliC gene |
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