DNA excision repair in mammalian cell extracts
The many genetic complementation groups of DNA excision‐repair defective mammalian cells indicate the considerable complexity of the excision repair process. The cloning of several repair genes is taking the field a step closer to mechanistic studies of the actions and interactions of repair protein...
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| Veröffentlicht in: | BioEssays 1991-09, Vol.13 (9), p.447-453 |
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| container_title | BioEssays |
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| creator | Wood, Richard D. Coverley, Dawn |
| description | The many genetic complementation groups of DNA excision‐repair defective mammalian cells indicate the considerable complexity of the excision repair process. The cloning of several repair genes is taking the field a step closer to mechanistic studies of the actions and interactions of repair proteins. Early biochemical studies of mammalian DNA repair in vitro are now at hand. Repair synthesis in damaged DNA can be monitored by following the incorporation of radiolabelled nucleotides. Synthesis is carried out by mammalian cell extracts and is defective in extracts from cell lines derived from individuals with the excisionrepair disorder xeroderma pigmentosum. Biochemical complementation of the defective extracts can be used to purify repair proteins. Repair of damage caused by agents including ultraviolet irradiation, psoralens, and platinating compounds has been observed. Neutralising antibodies against the human single‐stranded DNA binding protein (HSSB) have demonstrated a requirement for this protein in DNA excision repair as well as in DNA replication. |
| doi_str_mv | 10.1002/bies.950130904 |
| format | Article |
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The cloning of several repair genes is taking the field a step closer to mechanistic studies of the actions and interactions of repair proteins. Early biochemical studies of mammalian DNA repair in vitro are now at hand. Repair synthesis in damaged DNA can be monitored by following the incorporation of radiolabelled nucleotides. Synthesis is carried out by mammalian cell extracts and is defective in extracts from cell lines derived from individuals with the excisionrepair disorder xeroderma pigmentosum. Biochemical complementation of the defective extracts can be used to purify repair proteins. Repair of damage caused by agents including ultraviolet irradiation, psoralens, and platinating compounds has been observed. Neutralising antibodies against the human single‐stranded DNA binding protein (HSSB) have demonstrated a requirement for this protein in DNA excision repair as well as in DNA replication.</description><identifier>ISSN: 0265-9247</identifier><identifier>EISSN: 1521-1878</identifier><identifier>DOI: 10.1002/bies.950130904</identifier><identifier>PMID: 1796907</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Cell Line ; Cell-Free System ; DNA - metabolism ; DNA - radiation effects ; DNA Damage ; DNA Ligases - physiology ; DNA Repair ; DNA, Circular - metabolism ; DNA-Binding Proteins - physiology ; DNA-Directed RNA Polymerases - metabolism ; Genetic Complementation Test ; Humans ; Mammals - metabolism ; Models, Genetic ; Models, Molecular ; Plasmids ; Pyrimidine Dimers - metabolism ; Pyrimidines - radiation effects ; Replication Protein A ; Ultraviolet Rays ; Xeroderma Pigmentosum - classification ; Xeroderma Pigmentosum - genetics ; Xeroderma Pigmentosum - metabolism</subject><ispartof>BioEssays, 1991-09, Vol.13 (9), p.447-453</ispartof><rights>Copyright © 1991 Cambridge University Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3784-e00722a64de5e6efa6af4e9cbc60c43597439ffcecc86e7cac350ab235e747c33</citedby><cites>FETCH-LOGICAL-c3784-e00722a64de5e6efa6af4e9cbc60c43597439ffcecc86e7cac350ab235e747c33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbies.950130904$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbies.950130904$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1796907$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wood, Richard D.</creatorcontrib><creatorcontrib>Coverley, Dawn</creatorcontrib><title>DNA excision repair in mammalian cell extracts</title><title>BioEssays</title><addtitle>Bioessays</addtitle><description>The many genetic complementation groups of DNA excision‐repair defective mammalian cells indicate the considerable complexity of the excision repair process. The cloning of several repair genes is taking the field a step closer to mechanistic studies of the actions and interactions of repair proteins. Early biochemical studies of mammalian DNA repair in vitro are now at hand. Repair synthesis in damaged DNA can be monitored by following the incorporation of radiolabelled nucleotides. Synthesis is carried out by mammalian cell extracts and is defective in extracts from cell lines derived from individuals with the excisionrepair disorder xeroderma pigmentosum. Biochemical complementation of the defective extracts can be used to purify repair proteins. Repair of damage caused by agents including ultraviolet irradiation, psoralens, and platinating compounds has been observed. Neutralising antibodies against the human single‐stranded DNA binding protein (HSSB) have demonstrated a requirement for this protein in DNA excision repair as well as in DNA replication.</description><subject>Animals</subject><subject>Cell Line</subject><subject>Cell-Free System</subject><subject>DNA - metabolism</subject><subject>DNA - radiation effects</subject><subject>DNA Damage</subject><subject>DNA Ligases - physiology</subject><subject>DNA Repair</subject><subject>DNA, Circular - metabolism</subject><subject>DNA-Binding Proteins - physiology</subject><subject>DNA-Directed RNA Polymerases - metabolism</subject><subject>Genetic Complementation Test</subject><subject>Humans</subject><subject>Mammals - metabolism</subject><subject>Models, Genetic</subject><subject>Models, Molecular</subject><subject>Plasmids</subject><subject>Pyrimidine Dimers - metabolism</subject><subject>Pyrimidines - radiation effects</subject><subject>Replication Protein A</subject><subject>Ultraviolet Rays</subject><subject>Xeroderma Pigmentosum - classification</subject><subject>Xeroderma Pigmentosum - genetics</subject><subject>Xeroderma Pigmentosum - metabolism</subject><issn>0265-9247</issn><issn>1521-1878</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM9LAkEUx4cozKxrt2BP3dbezk_naFZqiREVQZdhHN_C1K7ajJL-962sWLfgwTu8z_fD40vIeQbtDIBeTTzGthaQMdDAD0gzEzRLs47qHJImUClSTbk6JicxfgCAlpQ3SCNTWmpQTdK-GXcTXDsf_XyWBFxYHxI_S0pblrbwdpY4LIqKWAbrlvGUHOW2iHi22y3yenf70huko8f-sNcdpY6pDk8RQFFqJZ-iQIm5lTbnqN3ESXCcCa0403nu0LmOROWsYwLshDKBiivHWItc1t5FmH-tMC5N6eP2EzvD-SoaRWU1VFRguwZdmMcYMDeL4EsbNiYDsy3IbAsy-4KqwMXOvJqUOP3F60aqu67v377AzT82cz28ff7rTuusj0tc77M2fBqpmBLmbdw394O7hyd4fzDAfgD6WIDb</recordid><startdate>199109</startdate><enddate>199109</enddate><creator>Wood, Richard D.</creator><creator>Coverley, Dawn</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199109</creationdate><title>DNA excision repair in mammalian cell extracts</title><author>Wood, Richard D. ; Coverley, Dawn</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3784-e00722a64de5e6efa6af4e9cbc60c43597439ffcecc86e7cac350ab235e747c33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>Cell Line</topic><topic>Cell-Free System</topic><topic>DNA - metabolism</topic><topic>DNA - radiation effects</topic><topic>DNA Damage</topic><topic>DNA Ligases - physiology</topic><topic>DNA Repair</topic><topic>DNA, Circular - metabolism</topic><topic>DNA-Binding Proteins - physiology</topic><topic>DNA-Directed RNA Polymerases - metabolism</topic><topic>Genetic Complementation Test</topic><topic>Humans</topic><topic>Mammals - metabolism</topic><topic>Models, Genetic</topic><topic>Models, Molecular</topic><topic>Plasmids</topic><topic>Pyrimidine Dimers - metabolism</topic><topic>Pyrimidines - radiation effects</topic><topic>Replication Protein A</topic><topic>Ultraviolet Rays</topic><topic>Xeroderma Pigmentosum - classification</topic><topic>Xeroderma Pigmentosum - genetics</topic><topic>Xeroderma Pigmentosum - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wood, Richard D.</creatorcontrib><creatorcontrib>Coverley, Dawn</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>BioEssays</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wood, Richard D.</au><au>Coverley, Dawn</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA excision repair in mammalian cell extracts</atitle><jtitle>BioEssays</jtitle><addtitle>Bioessays</addtitle><date>1991-09</date><risdate>1991</risdate><volume>13</volume><issue>9</issue><spage>447</spage><epage>453</epage><pages>447-453</pages><issn>0265-9247</issn><eissn>1521-1878</eissn><abstract>The many genetic complementation groups of DNA excision‐repair defective mammalian cells indicate the considerable complexity of the excision repair process. The cloning of several repair genes is taking the field a step closer to mechanistic studies of the actions and interactions of repair proteins. Early biochemical studies of mammalian DNA repair in vitro are now at hand. Repair synthesis in damaged DNA can be monitored by following the incorporation of radiolabelled nucleotides. Synthesis is carried out by mammalian cell extracts and is defective in extracts from cell lines derived from individuals with the excisionrepair disorder xeroderma pigmentosum. Biochemical complementation of the defective extracts can be used to purify repair proteins. Repair of damage caused by agents including ultraviolet irradiation, psoralens, and platinating compounds has been observed. Neutralising antibodies against the human single‐stranded DNA binding protein (HSSB) have demonstrated a requirement for this protein in DNA excision repair as well as in DNA replication.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>1796907</pmid><doi>10.1002/bies.950130904</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0265-9247 |
| ispartof | BioEssays, 1991-09, Vol.13 (9), p.447-453 |
| issn | 0265-9247 1521-1878 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72672625 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Cell Line Cell-Free System DNA - metabolism DNA - radiation effects DNA Damage DNA Ligases - physiology DNA Repair DNA, Circular - metabolism DNA-Binding Proteins - physiology DNA-Directed RNA Polymerases - metabolism Genetic Complementation Test Humans Mammals - metabolism Models, Genetic Models, Molecular Plasmids Pyrimidine Dimers - metabolism Pyrimidines - radiation effects Replication Protein A Ultraviolet Rays Xeroderma Pigmentosum - classification Xeroderma Pigmentosum - genetics Xeroderma Pigmentosum - metabolism |
| title | DNA excision repair in mammalian cell extracts |
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