Aryl hydrocarbon hydroxylase activity assayed in whole cell lysates using synchronous fluorescence spectroscopy

Synchronous fluorescence spectrophotometry has been used to measure induced and constitutive levels of aryl hydrocarbon hydroxylase activity in lysates of C3H 10T1 2 mouse embryo fibroblasts. Without compromising sensitivity, the method was reproducible, eliminated the need to extract metabolites, a...

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Veröffentlicht in:	Analytical biochemistry 1991-11, Vol.198 (2), p.355-362
Hauptverfasser:	Wells, R.L., Sneider, T.W., Elkind, M.M.
Format:	Artikel
Sprache:	eng
Schlagworte:	3-hydroxybenzo(a)pyrene Analytical, structural and metabolic biochemistry Animals Aryl Hydrocarbon Hydroxylases - chemistry assays Benzopyrenes Biological and medical sciences Cell Extracts - chemistry Cell Fractionation Cell Line enzymatic activity Enzymes and enzyme inhibitors fibroblasts Fibroblasts - enzymology Fundamental and applied biological sciences. Psychology measurements Mice Mice, Inbred C3H Oxidoreductases Spectrometry, Fluorescence Substrate Specificity unspecific monooxygenase
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