Characterization of covalent adducts to intact cytochrome P450s by mass spectrometry

This chapter focuses on (1) characterizing MBIs that attach covalently to an active site amino acid and (2) developing an analytical technique that would allow us to accomplish goals in an efficient and accurate manner. To this end, it chooses electrospray ionization (ESI)-mass spectrometry (MS) as...

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Veröffentlicht in:	Methods in Enzymology 2002, Vol.357, p.296-300
Hauptverfasser:	Lightning, Luke K., Trager, William F.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cytochrome P-450 Enzyme System - chemistry Cytochrome P-450 Enzyme System - isolation & purification Humans Methoxsalen - chemistry Recombinant Proteins - chemistry Spectrometry, Mass, Electrospray Ionization - methods
Online-Zugang:	Volltext
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