Effects of castration, dietary fat and adipose tissue sites on adipocyte plasma-membranes cyclic amp phosphodiesterase activity in the pig
1. 1. cAMP Phosphodiesterase activity and kinetic parameters ( K m , and V max ) were measured in subcutaneous and perirenal adipocyte plasma membranes from Large White male and castrated pigs. The animals were fed a control low fat diet or a sunflower diet enriched with linoleic acid (C18:2 n −6)....
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| Veröffentlicht in: | International journal of biochemistry 1991, Vol.23 (11), p.1205-1211 |
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| container_title | International journal of biochemistry |
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| creator | Benmansour, Moré Demarne, Yves Lecourtier, Marie-José Lhuillery, Claude |
| description | 1.
1. cAMP Phosphodiesterase activity and kinetic parameters (
K
m
, and
V
max
) were measured in subcutaneous and perirenal adipocyte plasma membranes from Large White male and castrated pigs. The animals were fed a control low fat diet or a sunflower diet enriched with linoleic acid (C18:2 n −6).
2.
2. Phosphodiesterase activity, low
K
m
and
V
max
were lowered by castration.
3.
3. In animals fed the sunflower diet, Phosphodiesterase activity decreased without affecting either
K
m
or
V
max
.
4.
4. Phosphodiesterase activity was higher in perirenal sites than in subcutaneous ones, particularly in male pigs. This may be explained by a lower
K
m
or a higher cAMP phosphodiesterase affinity to cAMP in perirenal sites.
5.
5. Theophylline was a potent inhibitor of phosphodiesterase activity principally in perirenal sites.
6.
6. The intermediate role of cAMP phosphodiesterase in adenylate cyclase activity and lipolytic processes is discussed. |
| doi_str_mv | 10.1016/0020-711X(91)90217-B |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72667576</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0020711X9190217B</els_id><sourcerecordid>72667576</sourcerecordid><originalsourceid>FETCH-LOGICAL-c405t-ba418535ca037ae62bf09d41592ba7a2839aad598a4c488748e27ce6f66cf5b23</originalsourceid><addsrcrecordid>eNp9kU1rFTEUhmeh1Fr9A6KQhUgFR5OZTDLZCLa0Wih0oQV34UzmpI3MV3NyC_cv-Kub61zanYsQyPvBy5OieCv4Z8GF-sJ5xUstxO9jIz4aXgldnjwrDh-fXxQvif5wLkwrxUFxIJRqZFUfFn_PvEeXiM2eOaAUIYV5-sT6gAnilnlIDKaeQR-WmZClQLRBRiFhzkzru9smZMsANEI54thFmLLqtm4IjsG4sOV2pnxyKSWMkHvApXAf0paFiaXbnA43r4rnHgbC1_v7qLg-P_t1-qO8vPp-cfrtsnSSN6nsQIq2qRsHvNaAquo8N70Ujak60FC1tQHoG9OCdLJttWyx0g6VV8r5pqvqo-LD2rvE-W6TF9kxkMNhyKvnDVldKaUbrbJRrkYXZ6KI3i4xjJmKFdzusNsdX7vja42w_7Dbkxx7t-_fdCP2T6GVedbf73UgB4PPtFygR5s0Rkgts-3NavMwW7iJ2XL904hWtmY37esqYgZ1HzBacgEnh32I-TttP4f_j3wADQGrkw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72667576</pqid></control><display><type>article</type><title>Effects of castration, dietary fat and adipose tissue sites on adipocyte plasma-membranes cyclic amp phosphodiesterase activity in the pig</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Benmansour, Moré ; Demarne, Yves ; Lecourtier, Marie-José ; Lhuillery, Claude</creator><creatorcontrib>Benmansour, Moré ; Demarne, Yves ; Lecourtier, Marie-José ; Lhuillery, Claude</creatorcontrib><description>1.
1. cAMP Phosphodiesterase activity and kinetic parameters (
K
m
, and
V
max
) were measured in subcutaneous and perirenal adipocyte plasma membranes from Large White male and castrated pigs. The animals were fed a control low fat diet or a sunflower diet enriched with linoleic acid (C18:2 n −6).
2.
2. Phosphodiesterase activity, low
K
m
and
V
max
were lowered by castration.
3.
3. In animals fed the sunflower diet, Phosphodiesterase activity decreased without affecting either
K
m
or
V
max
.
4.
4. Phosphodiesterase activity was higher in perirenal sites than in subcutaneous ones, particularly in male pigs. This may be explained by a lower
K
m
or a higher cAMP phosphodiesterase affinity to cAMP in perirenal sites.
5.
5. Theophylline was a potent inhibitor of phosphodiesterase activity principally in perirenal sites.
6.
6. The intermediate role of cAMP phosphodiesterase in adenylate cyclase activity and lipolytic processes is discussed.</description><identifier>ISSN: 0020-711X</identifier><identifier>DOI: 10.1016/0020-711X(91)90217-B</identifier><identifier>PMID: 1665423</identifier><language>eng</language><publisher>Oxford: Elsevier B.V</publisher><subject>3',5'-Cyclic-AMP Phosphodiesterases - metabolism ; ACIDE LINOLEIQUE ; ACIDO LINOLEICO ; ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; ADENOSINE MONOPHOSPHATE ; ADENOSINMONOFOSFATO ; Adipose Tissue - cytology ; Adipose Tissue - drug effects ; Adipose Tissue - metabolism ; Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; CASTRACION ; CASTRATION ; Cell Membrane - enzymology ; CELLULE ; CELULAS ; CERDO ; CORPS GRAS ; DIETA ; Dietary Fats - pharmacology ; Enzymes and enzyme inhibitors ; ESTERASAS ; ESTERASE ; ESTRUCTURA CELULAR ; Fundamental and applied biological sciences. Psychology ; GRASAS ; Hydrolases ; Kidney - cytology ; Kidney - metabolism ; Linoleic Acid ; Linoleic Acids ; Male ; Plant Oils ; PORCIN ; REGIME ALIMENTAIRE ; Skin - cytology ; Skin - metabolism ; STRUCTURE CELLULAIRE ; Sunflower Oil ; Swine ; TEJIDO ADIPOSO ; TEOFILINA ; Testis - physiology ; THEOPHYLLINE ; Theophylline - pharmacology ; TISSU ADIPEUX</subject><ispartof>International journal of biochemistry, 1991, Vol.23 (11), p.1205-1211</ispartof><rights>1991</rights><rights>1992 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-ba418535ca037ae62bf09d41592ba7a2839aad598a4c488748e27ce6f66cf5b23</citedby><cites>FETCH-LOGICAL-c405t-ba418535ca037ae62bf09d41592ba7a2839aad598a4c488748e27ce6f66cf5b23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27902,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4991474$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1665423$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Benmansour, Moré</creatorcontrib><creatorcontrib>Demarne, Yves</creatorcontrib><creatorcontrib>Lecourtier, Marie-José</creatorcontrib><creatorcontrib>Lhuillery, Claude</creatorcontrib><title>Effects of castration, dietary fat and adipose tissue sites on adipocyte plasma-membranes cyclic amp phosphodiesterase activity in the pig</title><title>International journal of biochemistry</title><addtitle>Int J Biochem</addtitle><description>1.
1. cAMP Phosphodiesterase activity and kinetic parameters (
K
m
, and
V
max
) were measured in subcutaneous and perirenal adipocyte plasma membranes from Large White male and castrated pigs. The animals were fed a control low fat diet or a sunflower diet enriched with linoleic acid (C18:2 n −6).
2.
2. Phosphodiesterase activity, low
K
m
and
V
max
were lowered by castration.
3.
3. In animals fed the sunflower diet, Phosphodiesterase activity decreased without affecting either
K
m
or
V
max
.
4.
4. Phosphodiesterase activity was higher in perirenal sites than in subcutaneous ones, particularly in male pigs. This may be explained by a lower
K
m
or a higher cAMP phosphodiesterase affinity to cAMP in perirenal sites.
5.
5. Theophylline was a potent inhibitor of phosphodiesterase activity principally in perirenal sites.
6.
6. The intermediate role of cAMP phosphodiesterase in adenylate cyclase activity and lipolytic processes is discussed.</description><subject>3',5'-Cyclic-AMP Phosphodiesterases - metabolism</subject><subject>ACIDE LINOLEIQUE</subject><subject>ACIDO LINOLEICO</subject><subject>ACTIVIDAD ENZIMATICA</subject><subject>ACTIVITE ENZYMATIQUE</subject><subject>ADENOSINE MONOPHOSPHATE</subject><subject>ADENOSINMONOFOSFATO</subject><subject>Adipose Tissue - cytology</subject><subject>Adipose Tissue - drug effects</subject><subject>Adipose Tissue - metabolism</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>CASTRACION</subject><subject>CASTRATION</subject><subject>Cell Membrane - enzymology</subject><subject>CELLULE</subject><subject>CELULAS</subject><subject>CERDO</subject><subject>CORPS GRAS</subject><subject>DIETA</subject><subject>Dietary Fats - pharmacology</subject><subject>Enzymes and enzyme inhibitors</subject><subject>ESTERASAS</subject><subject>ESTERASE</subject><subject>ESTRUCTURA CELULAR</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GRASAS</subject><subject>Hydrolases</subject><subject>Kidney - cytology</subject><subject>Kidney - metabolism</subject><subject>Linoleic Acid</subject><subject>Linoleic Acids</subject><subject>Male</subject><subject>Plant Oils</subject><subject>PORCIN</subject><subject>REGIME ALIMENTAIRE</subject><subject>Skin - cytology</subject><subject>Skin - metabolism</subject><subject>STRUCTURE CELLULAIRE</subject><subject>Sunflower Oil</subject><subject>Swine</subject><subject>TEJIDO ADIPOSO</subject><subject>TEOFILINA</subject><subject>Testis - physiology</subject><subject>THEOPHYLLINE</subject><subject>Theophylline - pharmacology</subject><subject>TISSU ADIPEUX</subject><issn>0020-711X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1rFTEUhmeh1Fr9A6KQhUgFR5OZTDLZCLa0Wih0oQV34UzmpI3MV3NyC_cv-Kub61zanYsQyPvBy5OieCv4Z8GF-sJ5xUstxO9jIz4aXgldnjwrDh-fXxQvif5wLkwrxUFxIJRqZFUfFn_PvEeXiM2eOaAUIYV5-sT6gAnilnlIDKaeQR-WmZClQLRBRiFhzkzru9smZMsANEI54thFmLLqtm4IjsG4sOV2pnxyKSWMkHvApXAf0paFiaXbnA43r4rnHgbC1_v7qLg-P_t1-qO8vPp-cfrtsnSSN6nsQIq2qRsHvNaAquo8N70Ujak60FC1tQHoG9OCdLJttWyx0g6VV8r5pqvqo-LD2rvE-W6TF9kxkMNhyKvnDVldKaUbrbJRrkYXZ6KI3i4xjJmKFdzusNsdX7vja42w_7Dbkxx7t-_fdCP2T6GVedbf73UgB4PPtFygR5s0Rkgts-3NavMwW7iJ2XL904hWtmY37esqYgZ1HzBacgEnh32I-TttP4f_j3wADQGrkw</recordid><startdate>1991</startdate><enddate>1991</enddate><creator>Benmansour, Moré</creator><creator>Demarne, Yves</creator><creator>Lecourtier, Marie-José</creator><creator>Lhuillery, Claude</creator><general>Elsevier B.V</general><general>Pergamon</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1991</creationdate><title>Effects of castration, dietary fat and adipose tissue sites on adipocyte plasma-membranes cyclic amp phosphodiesterase activity in the pig</title><author>Benmansour, Moré ; Demarne, Yves ; Lecourtier, Marie-José ; Lhuillery, Claude</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-ba418535ca037ae62bf09d41592ba7a2839aad598a4c488748e27ce6f66cf5b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>3',5'-Cyclic-AMP Phosphodiesterases - metabolism</topic><topic>ACIDE LINOLEIQUE</topic><topic>ACIDO LINOLEICO</topic><topic>ACTIVIDAD ENZIMATICA</topic><topic>ACTIVITE ENZYMATIQUE</topic><topic>ADENOSINE MONOPHOSPHATE</topic><topic>ADENOSINMONOFOSFATO</topic><topic>Adipose Tissue - cytology</topic><topic>Adipose Tissue - drug effects</topic><topic>Adipose Tissue - metabolism</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>CASTRACION</topic><topic>CASTRATION</topic><topic>Cell Membrane - enzymology</topic><topic>CELLULE</topic><topic>CELULAS</topic><topic>CERDO</topic><topic>CORPS GRAS</topic><topic>DIETA</topic><topic>Dietary Fats - pharmacology</topic><topic>Enzymes and enzyme inhibitors</topic><topic>ESTERASAS</topic><topic>ESTERASE</topic><topic>ESTRUCTURA CELULAR</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GRASAS</topic><topic>Hydrolases</topic><topic>Kidney - cytology</topic><topic>Kidney - metabolism</topic><topic>Linoleic Acid</topic><topic>Linoleic Acids</topic><topic>Male</topic><topic>Plant Oils</topic><topic>PORCIN</topic><topic>REGIME ALIMENTAIRE</topic><topic>Skin - cytology</topic><topic>Skin - metabolism</topic><topic>STRUCTURE CELLULAIRE</topic><topic>Sunflower Oil</topic><topic>Swine</topic><topic>TEJIDO ADIPOSO</topic><topic>TEOFILINA</topic><topic>Testis - physiology</topic><topic>THEOPHYLLINE</topic><topic>Theophylline - pharmacology</topic><topic>TISSU ADIPEUX</topic><toplevel>online_resources</toplevel><creatorcontrib>Benmansour, Moré</creatorcontrib><creatorcontrib>Demarne, Yves</creatorcontrib><creatorcontrib>Lecourtier, Marie-José</creatorcontrib><creatorcontrib>Lhuillery, Claude</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Benmansour, Moré</au><au>Demarne, Yves</au><au>Lecourtier, Marie-José</au><au>Lhuillery, Claude</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of castration, dietary fat and adipose tissue sites on adipocyte plasma-membranes cyclic amp phosphodiesterase activity in the pig</atitle><jtitle>International journal of biochemistry</jtitle><addtitle>Int J Biochem</addtitle><date>1991</date><risdate>1991</risdate><volume>23</volume><issue>11</issue><spage>1205</spage><epage>1211</epage><pages>1205-1211</pages><issn>0020-711X</issn><abstract>1.
1. cAMP Phosphodiesterase activity and kinetic parameters (
K
m
, and
V
max
) were measured in subcutaneous and perirenal adipocyte plasma membranes from Large White male and castrated pigs. The animals were fed a control low fat diet or a sunflower diet enriched with linoleic acid (C18:2 n −6).
2.
2. Phosphodiesterase activity, low
K
m
and
V
max
were lowered by castration.
3.
3. In animals fed the sunflower diet, Phosphodiesterase activity decreased without affecting either
K
m
or
V
max
.
4.
4. Phosphodiesterase activity was higher in perirenal sites than in subcutaneous ones, particularly in male pigs. This may be explained by a lower
K
m
or a higher cAMP phosphodiesterase affinity to cAMP in perirenal sites.
5.
5. Theophylline was a potent inhibitor of phosphodiesterase activity principally in perirenal sites.
6.
6. The intermediate role of cAMP phosphodiesterase in adenylate cyclase activity and lipolytic processes is discussed.</abstract><cop>Oxford</cop><pub>Elsevier B.V</pub><pmid>1665423</pmid><doi>10.1016/0020-711X(91)90217-B</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0020-711X |
| ispartof | International journal of biochemistry, 1991, Vol.23 (11), p.1205-1211 |
| issn | 0020-711X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72667576 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | 3',5'-Cyclic-AMP Phosphodiesterases - metabolism ACIDE LINOLEIQUE ACIDO LINOLEICO ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE ADENOSINE MONOPHOSPHATE ADENOSINMONOFOSFATO Adipose Tissue - cytology Adipose Tissue - drug effects Adipose Tissue - metabolism Analytical, structural and metabolic biochemistry Animals Biological and medical sciences CASTRACION CASTRATION Cell Membrane - enzymology CELLULE CELULAS CERDO CORPS GRAS DIETA Dietary Fats - pharmacology Enzymes and enzyme inhibitors ESTERASAS ESTERASE ESTRUCTURA CELULAR Fundamental and applied biological sciences. Psychology GRASAS Hydrolases Kidney - cytology Kidney - metabolism Linoleic Acid Linoleic Acids Male Plant Oils PORCIN REGIME ALIMENTAIRE Skin - cytology Skin - metabolism STRUCTURE CELLULAIRE Sunflower Oil Swine TEJIDO ADIPOSO TEOFILINA Testis - physiology THEOPHYLLINE Theophylline - pharmacology TISSU ADIPEUX |
| title | Effects of castration, dietary fat and adipose tissue sites on adipocyte plasma-membranes cyclic amp phosphodiesterase activity in the pig |
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