Purification of surfactant protein A from dog lung by reconstitution with surfactant lipids

We have developed a simple method for purification of surfactant major apoprotein (SP-A, MW 34–38 kD) from dog lungs with high yield and purity. Lipids and proteins of partially purified surfactant were dissociated by sodium deoxycholate (DOC, 100 mM, 37°C, 30 min), diluted 1:10 with borate buffer c...

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Veröffentlicht in:Chemistry and physics of lipids 1991-08, Vol.59 (1), p.29-38
Hauptverfasser: Liau, Deng F., Ryan, Stephen F.
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Sprache:eng
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