H‐DNA formation by the coding repeat elements of neisserial opa genes
Summary The coding repeat region of opa genes from Neisseria gonorrhoeae and Neisseria meningitidis determines the expression state of their respective genes through high‐frequency addition or deletion of pentanucleotide coding repeat units (CRs; CTTCT). In vitro analyses of cloned opa gene CR regio...
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| Veröffentlicht in: | Molecular microbiology 1991-10, Vol.5 (10), p.2351-2360 |
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| creator | Belland, R. J. |
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The coding repeat region of opa genes from Neisseria gonorrhoeae and Neisseria meningitidis determines the expression state of their respective genes through high‐frequency addition or deletion of pentanucleotide coding repeat units (CRs; CTTCT). In vitro analyses of cloned opa gene CR regions using single‐strand specific nucleases, oligonucleotide protection experiments, and modifications of non‐B‐DNA residues indicate that the regions form structures resembling H‐DNA under acidic conditions in the presence of negative supercoiling. The purine/pyrimidine strand bias and H‐palindromic nature of the repeat region are consistent with sequence requirements for H‐DNA formation. Sequences flanking the repeat elements are required to form the H‐DNA structure in vitro as judged by the pattern of exposed non‐B‐DNA residues in CR sequences synthesized as oligonucleotides to form β‐galactosidase::CR translational fusions. The fusions phase vary by addition and deletion of CR elements and the rate of phase variation increases upon induction of the fusion genes. The opa gene CR region is the first reported bacterial H‐DNA structure and is unique in that it ties within the coding sequence for the gene. |
| doi_str_mv | 10.1111/j.1365-2958.1991.tb02081.x |
| format | Article |
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The coding repeat region of opa genes from Neisseria gonorrhoeae and Neisseria meningitidis determines the expression state of their respective genes through high‐frequency addition or deletion of pentanucleotide coding repeat units (CRs; CTTCT). In vitro analyses of cloned opa gene CR regions using single‐strand specific nucleases, oligonucleotide protection experiments, and modifications of non‐B‐DNA residues indicate that the regions form structures resembling H‐DNA under acidic conditions in the presence of negative supercoiling. The purine/pyrimidine strand bias and H‐palindromic nature of the repeat region are consistent with sequence requirements for H‐DNA formation. Sequences flanking the repeat elements are required to form the H‐DNA structure in vitro as judged by the pattern of exposed non‐B‐DNA residues in CR sequences synthesized as oligonucleotides to form β‐galactosidase::CR translational fusions. The fusions phase vary by addition and deletion of CR elements and the rate of phase variation increases upon induction of the fusion genes. The opa gene CR region is the first reported bacterial H‐DNA structure and is unique in that it ties within the coding sequence for the gene.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1111/j.1365-2958.1991.tb02081.x</identifier><identifier>PMID: 1791751</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Amino Acid Sequence ; Bacteriology ; Base Sequence ; beta-Galactosidase - genetics ; beta-Galactosidase - metabolism ; Biological and medical sciences ; DNA - genetics ; DNA - isolation & purification ; DNA, Bacterial - genetics ; DNA, Bacterial - isolation & purification ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Bacterial ; Genetics ; Microbiology ; Molecular Sequence Data ; Neisseria gonorrhoeae ; Neisseria gonorrhoeae - genetics ; Neisseria meningitidis ; Neisseria meningitidis - genetics ; Nucleic Acid Conformation ; Oligodeoxyribonucleotides ; Recombinant Fusion Proteins - metabolism ; Repetitive Sequences, Nucleic Acid ; Restriction Mapping</subject><ispartof>Molecular microbiology, 1991-10, Vol.5 (10), p.2351-2360</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4301-c896fecba63d02bfdf9529cf3d6ffa905c4a581bc9b209273cadf578e4a364953</citedby><cites>FETCH-LOGICAL-c4301-c896fecba63d02bfdf9529cf3d6ffa905c4a581bc9b209273cadf578e4a364953</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2958.1991.tb02081.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2958.1991.tb02081.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5072856$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1791751$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Belland, R. J.</creatorcontrib><title>H‐DNA formation by the coding repeat elements of neisserial opa genes</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>Summary
The coding repeat region of opa genes from Neisseria gonorrhoeae and Neisseria meningitidis determines the expression state of their respective genes through high‐frequency addition or deletion of pentanucleotide coding repeat units (CRs; CTTCT). In vitro analyses of cloned opa gene CR regions using single‐strand specific nucleases, oligonucleotide protection experiments, and modifications of non‐B‐DNA residues indicate that the regions form structures resembling H‐DNA under acidic conditions in the presence of negative supercoiling. The purine/pyrimidine strand bias and H‐palindromic nature of the repeat region are consistent with sequence requirements for H‐DNA formation. Sequences flanking the repeat elements are required to form the H‐DNA structure in vitro as judged by the pattern of exposed non‐B‐DNA residues in CR sequences synthesized as oligonucleotides to form β‐galactosidase::CR translational fusions. The fusions phase vary by addition and deletion of CR elements and the rate of phase variation increases upon induction of the fusion genes. The opa gene CR region is the first reported bacterial H‐DNA structure and is unique in that it ties within the coding sequence for the gene.</description><subject>Amino Acid Sequence</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>beta-Galactosidase - genetics</subject><subject>beta-Galactosidase - metabolism</subject><subject>Biological and medical sciences</subject><subject>DNA - genetics</subject><subject>DNA - isolation & purification</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genetics</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Neisseria gonorrhoeae</subject><subject>Neisseria gonorrhoeae - genetics</subject><subject>Neisseria meningitidis</subject><subject>Neisseria meningitidis - genetics</subject><subject>Nucleic Acid Conformation</subject><subject>Oligodeoxyribonucleotides</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>Restriction Mapping</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkMlOwzAQhi0EgrI8ApKFELcEL7ETc0EVWyuxXEDiZjnOGFJlKXYq6I1H4Bl5ElJawRExlzn83yz6EDqgJKZ9HU9iyqWImBJZTJWicZcTRjIav62hwU-0jgZECRLxjD1uoe0QJoRQTiTfRJs0VTQVdICuRp_vH-e3Q-xaX5uubBucz3H3DNi2Rdk8YQ9TMB2GCmpouoBbhxsoQwBfmgq3U4OfoIGwizacqQLsrfoOeri8uD8bRdd3V-Oz4XVkE05oZDMlHdjcSF4QlrvCKcGUdbyQzhlFhE2MyGhuVc6IYim3pnAizSAxXCZK8B10tNw79e3LDEKn6zJYqCrTQDsLOmVS0kSlf4JUMpXxb_BkCVrfhuDB6akva-PnmhK90K0neuFUL5zqhW690q3f-uH91ZVZXkPxO7r02-eHq9wEayrnTWPL8IMJkrJMyB47XWKvZQXzfzygb27GjPeHvgB8iJ03</recordid><startdate>199110</startdate><enddate>199110</enddate><creator>Belland, R. J.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>199110</creationdate><title>H‐DNA formation by the coding repeat elements of neisserial opa genes</title><author>Belland, R. J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4301-c896fecba63d02bfdf9529cf3d6ffa905c4a581bc9b209273cadf578e4a364953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Amino Acid Sequence</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>beta-Galactosidase - genetics</topic><topic>beta-Galactosidase - metabolism</topic><topic>Biological and medical sciences</topic><topic>DNA - genetics</topic><topic>DNA - isolation & purification</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Bacterial - isolation & purification</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genetics</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Neisseria gonorrhoeae</topic><topic>Neisseria gonorrhoeae - genetics</topic><topic>Neisseria meningitidis</topic><topic>Neisseria meningitidis - genetics</topic><topic>Nucleic Acid Conformation</topic><topic>Oligodeoxyribonucleotides</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>Restriction Mapping</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Belland, R. J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Belland, R. J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>H‐DNA formation by the coding repeat elements of neisserial opa genes</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>1991-10</date><risdate>1991</risdate><volume>5</volume><issue>10</issue><spage>2351</spage><epage>2360</epage><pages>2351-2360</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>Summary
The coding repeat region of opa genes from Neisseria gonorrhoeae and Neisseria meningitidis determines the expression state of their respective genes through high‐frequency addition or deletion of pentanucleotide coding repeat units (CRs; CTTCT). In vitro analyses of cloned opa gene CR regions using single‐strand specific nucleases, oligonucleotide protection experiments, and modifications of non‐B‐DNA residues indicate that the regions form structures resembling H‐DNA under acidic conditions in the presence of negative supercoiling. The purine/pyrimidine strand bias and H‐palindromic nature of the repeat region are consistent with sequence requirements for H‐DNA formation. Sequences flanking the repeat elements are required to form the H‐DNA structure in vitro as judged by the pattern of exposed non‐B‐DNA residues in CR sequences synthesized as oligonucleotides to form β‐galactosidase::CR translational fusions. The fusions phase vary by addition and deletion of CR elements and the rate of phase variation increases upon induction of the fusion genes. The opa gene CR region is the first reported bacterial H‐DNA structure and is unique in that it ties within the coding sequence for the gene.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>1791751</pmid><doi>10.1111/j.1365-2958.1991.tb02081.x</doi><tpages>10</tpages></addata></record> |
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| subjects | Amino Acid Sequence Bacteriology Base Sequence beta-Galactosidase - genetics beta-Galactosidase - metabolism Biological and medical sciences DNA - genetics DNA - isolation & purification DNA, Bacterial - genetics DNA, Bacterial - isolation & purification Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial Genetics Microbiology Molecular Sequence Data Neisseria gonorrhoeae Neisseria gonorrhoeae - genetics Neisseria meningitidis Neisseria meningitidis - genetics Nucleic Acid Conformation Oligodeoxyribonucleotides Recombinant Fusion Proteins - metabolism Repetitive Sequences, Nucleic Acid Restriction Mapping |
| title | H‐DNA formation by the coding repeat elements of neisserial opa genes |
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