Release of Peptide Promoted by the GGQ Motif of Class 1 Release Factors Regulates the GTPase Activity of RF3

E. coli mutants of RF1 and RF2, in which the universal GGQ motif is changed to GAQ, are slow in peptide release from ribosomes. Other kinetic properties are unchanged, suggesting that the GGQ motif is in contact with the peptidyl-transferase center. Deacylated tRNA terminates protein synthesis codon...

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Veröffentlicht in:	Molecular cell 2002-10, Vol.10 (4), p.789-798
Hauptverfasser:	Zavialov, Andrei V, Mora, Liliana, Buckingham, Richard H, Ehrenberg, Måns
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Motifs Amino Acid Sequence Binding Sites Catalysis Codon, Terminator Escherichia coli - genetics Escherichia coli Proteins Gene Expression Regulation, Bacterial GTP Phosphohydrolases - metabolism Guanosine Diphosphate - metabolism Guanosine Triphosphate - metabolism Hydrolysis Kinetics Mutation Peptide Termination Factors - chemistry Peptide Termination Factors - genetics Peptide Termination Factors - metabolism Protein Binding Protein Biosynthesis Ribosomes - chemistry Ribosomes - metabolism RNA, Transfer - metabolism
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