A tissue inhibitor of metalloproteinases and alpha-macroglobulins in the ovulating rat ovary: possible regulators of collagen matrix breakdown
Immature female Sprague-Dawley rats were primed with 20 IU eCG at 28 days of age and treated with 10 IU hCG 48 h later. Ovulation followed at 12-14 h. Ovaries were extracted at various times after hCG by use of Triton X-100 and 10 mM CaCl2 (Triton extract) followed by heating to 60 degrees C for 6 m...
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| Veröffentlicht in: | Biology of reproduction 1991-08, Vol.45 (2), p.334-342 |
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| creator | C Zhu J F Woessner, Jr |
| description | Immature female Sprague-Dawley rats were primed with 20 IU eCG at 28 days of age and treated with 10 IU hCG 48 h later. Ovulation
followed at 12-14 h. Ovaries were extracted at various times after hCG by use of Triton X-100 and 10 mM CaCl2 (Triton extract)
followed by heating to 60 degrees C for 6 min with 0.1 M CaCl2 in 50 mM Tris/0.15 M NaCl, pH 7.5 (heat extract). These extracts
were tested for their ability to inhibit tissue metalloproteinases by use of the small uterine metalloproteinase (UMP) of
the rat. The ovary contains three plasma-derived inhibitors (alpha 1-macroglobulin [alpha 1 M], alpha 2-macroglobulin [alpha
2 M], and alpha 1 inhibitor3 [alpha 1I3]) and one tissue-derived inhibitor of the tissue inhibitor of metalloproteinases (TIMP)
family. alpha 1 I3 was shown to inhibit UMP and rat collagenase. The concentration of the tissue inhibitor rose 5-fold from
0.6 micrograms UMP blocked per gram wet tissue in ovaries not primed with eCG to 3.2 micrograms UMP blocked at 8 h after hCG.
Over this same time interval, the sum of alpha 1M + alpha 2M per gram of ovary rose 7-fold from 3.2 to 22.4 micrograms UMP
inhibited and alpha 1I3 rose 2-fold from 4.4 to 10.7 micrograms UMP inhibited. The increases in the tissue inhibitor are interpreted
to be due to increased synthesis by the tissue, whereas the changes in alpha-macroglobulins are postulated to be due to increased
vascularity and increased permeability of the vessels. |
| doi_str_mv | 10.1095/biolreprod45.2.334 |
| format | Article |
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followed at 12-14 h. Ovaries were extracted at various times after hCG by use of Triton X-100 and 10 mM CaCl2 (Triton extract)
followed by heating to 60 degrees C for 6 min with 0.1 M CaCl2 in 50 mM Tris/0.15 M NaCl, pH 7.5 (heat extract). These extracts
were tested for their ability to inhibit tissue metalloproteinases by use of the small uterine metalloproteinase (UMP) of
the rat. The ovary contains three plasma-derived inhibitors (alpha 1-macroglobulin [alpha 1 M], alpha 2-macroglobulin [alpha
2 M], and alpha 1 inhibitor3 [alpha 1I3]) and one tissue-derived inhibitor of the tissue inhibitor of metalloproteinases (TIMP)
family. alpha 1 I3 was shown to inhibit UMP and rat collagenase. The concentration of the tissue inhibitor rose 5-fold from
0.6 micrograms UMP blocked per gram wet tissue in ovaries not primed with eCG to 3.2 micrograms UMP blocked at 8 h after hCG.
Over this same time interval, the sum of alpha 1M + alpha 2M per gram of ovary rose 7-fold from 3.2 to 22.4 micrograms UMP
inhibited and alpha 1I3 rose 2-fold from 4.4 to 10.7 micrograms UMP inhibited. The increases in the tissue inhibitor are interpreted
to be due to increased synthesis by the tissue, whereas the changes in alpha-macroglobulins are postulated to be due to increased
vascularity and increased permeability of the vessels.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod45.2.334</identifier><identifier>PMID: 1723897</identifier><language>eng</language><publisher>United States: Society for the Study of Reproduction</publisher><subject>alpha-Macroglobulins - isolation & purification ; alpha-Macroglobulins - physiology ; Animals ; Collagen - metabolism ; Extracellular Matrix - metabolism ; Female ; Glycoproteins - isolation & purification ; Glycoproteins - physiology ; Hot Temperature ; Metalloendopeptidases - antagonists & inhibitors ; Ovary - chemistry ; Ovulation ; Protein Denaturation ; Rats ; Rats, Inbred Strains ; Tissue Inhibitor of Metalloproteinases</subject><ispartof>Biology of reproduction, 1991-08, Vol.45 (2), p.334-342</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1723897$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>C Zhu</creatorcontrib><creatorcontrib>J F Woessner, Jr</creatorcontrib><title>A tissue inhibitor of metalloproteinases and alpha-macroglobulins in the ovulating rat ovary: possible regulators of collagen matrix breakdown</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>Immature female Sprague-Dawley rats were primed with 20 IU eCG at 28 days of age and treated with 10 IU hCG 48 h later. Ovulation
followed at 12-14 h. Ovaries were extracted at various times after hCG by use of Triton X-100 and 10 mM CaCl2 (Triton extract)
followed by heating to 60 degrees C for 6 min with 0.1 M CaCl2 in 50 mM Tris/0.15 M NaCl, pH 7.5 (heat extract). These extracts
were tested for their ability to inhibit tissue metalloproteinases by use of the small uterine metalloproteinase (UMP) of
the rat. The ovary contains three plasma-derived inhibitors (alpha 1-macroglobulin [alpha 1 M], alpha 2-macroglobulin [alpha
2 M], and alpha 1 inhibitor3 [alpha 1I3]) and one tissue-derived inhibitor of the tissue inhibitor of metalloproteinases (TIMP)
family. alpha 1 I3 was shown to inhibit UMP and rat collagenase. The concentration of the tissue inhibitor rose 5-fold from
0.6 micrograms UMP blocked per gram wet tissue in ovaries not primed with eCG to 3.2 micrograms UMP blocked at 8 h after hCG.
Over this same time interval, the sum of alpha 1M + alpha 2M per gram of ovary rose 7-fold from 3.2 to 22.4 micrograms UMP
inhibited and alpha 1I3 rose 2-fold from 4.4 to 10.7 micrograms UMP inhibited. The increases in the tissue inhibitor are interpreted
to be due to increased synthesis by the tissue, whereas the changes in alpha-macroglobulins are postulated to be due to increased
vascularity and increased permeability of the vessels.</description><subject>alpha-Macroglobulins - isolation & purification</subject><subject>alpha-Macroglobulins - physiology</subject><subject>Animals</subject><subject>Collagen - metabolism</subject><subject>Extracellular Matrix - metabolism</subject><subject>Female</subject><subject>Glycoproteins - isolation & purification</subject><subject>Glycoproteins - physiology</subject><subject>Hot Temperature</subject><subject>Metalloendopeptidases - antagonists & inhibitors</subject><subject>Ovary - chemistry</subject><subject>Ovulation</subject><subject>Protein Denaturation</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Tissue Inhibitor of Metalloproteinases</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNotUctO3TAQtaoiuIX-ABKSN2WXS-zJw2GHEH1ISN2062icTBK3TnyxHUJ_ot-MEVezGI3OmaM5Zxi7FPle5E15o42zng7e9UW5l3uA4gPbiVI2WS0r9ZHt8jyvMoAKztinEP7kuShAwik7FbUE1dQ79v-ORxPCStwsk9EmOs_dwGeKaK1L0pHMgoECx6XnaA8TZjN23o3W6dWaJaRFHifi7nm1GM0yco8xTej_3fKDC8FoS9zT-AY7H97kO2ctjrTwGaM3L1x7wr-925YLdjKgDfT52M_Z768Pv-6_Z48_v_24v3vMJglVzGRDoIehk4QKlYSio1QSBxKq7-paaeqLQRdDrwGxwb5SnaigFgoqoXUD5-z6XTc5fFopxHY2oaN01UJuDW3Kr6xA5Yl4dSSueqa-PXgzJ2ftMcCEf3nHJzNOm_HUhjkll9jQbttWlK1s01_gFeABh4U</recordid><startdate>19910801</startdate><enddate>19910801</enddate><creator>C Zhu</creator><creator>J F Woessner, Jr</creator><general>Society for the Study of Reproduction</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19910801</creationdate><title>A tissue inhibitor of metalloproteinases and alpha-macroglobulins in the ovulating rat ovary: possible regulators of collagen matrix breakdown</title><author>C Zhu ; J F Woessner, Jr</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h236t-29e3bffc2ea8a8234cecec2afe18dc778bed4fb4fdb3aa9ad68c163718361bb93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>alpha-Macroglobulins - isolation & purification</topic><topic>alpha-Macroglobulins - physiology</topic><topic>Animals</topic><topic>Collagen - metabolism</topic><topic>Extracellular Matrix - metabolism</topic><topic>Female</topic><topic>Glycoproteins - isolation & purification</topic><topic>Glycoproteins - physiology</topic><topic>Hot Temperature</topic><topic>Metalloendopeptidases - antagonists & inhibitors</topic><topic>Ovary - chemistry</topic><topic>Ovulation</topic><topic>Protein Denaturation</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Tissue Inhibitor of Metalloproteinases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>C Zhu</creatorcontrib><creatorcontrib>J F Woessner, Jr</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>C Zhu</au><au>J F Woessner, Jr</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A tissue inhibitor of metalloproteinases and alpha-macroglobulins in the ovulating rat ovary: possible regulators of collagen matrix breakdown</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>1991-08-01</date><risdate>1991</risdate><volume>45</volume><issue>2</issue><spage>334</spage><epage>342</epage><pages>334-342</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><abstract>Immature female Sprague-Dawley rats were primed with 20 IU eCG at 28 days of age and treated with 10 IU hCG 48 h later. Ovulation
followed at 12-14 h. Ovaries were extracted at various times after hCG by use of Triton X-100 and 10 mM CaCl2 (Triton extract)
followed by heating to 60 degrees C for 6 min with 0.1 M CaCl2 in 50 mM Tris/0.15 M NaCl, pH 7.5 (heat extract). These extracts
were tested for their ability to inhibit tissue metalloproteinases by use of the small uterine metalloproteinase (UMP) of
the rat. The ovary contains three plasma-derived inhibitors (alpha 1-macroglobulin [alpha 1 M], alpha 2-macroglobulin [alpha
2 M], and alpha 1 inhibitor3 [alpha 1I3]) and one tissue-derived inhibitor of the tissue inhibitor of metalloproteinases (TIMP)
family. alpha 1 I3 was shown to inhibit UMP and rat collagenase. The concentration of the tissue inhibitor rose 5-fold from
0.6 micrograms UMP blocked per gram wet tissue in ovaries not primed with eCG to 3.2 micrograms UMP blocked at 8 h after hCG.
Over this same time interval, the sum of alpha 1M + alpha 2M per gram of ovary rose 7-fold from 3.2 to 22.4 micrograms UMP
inhibited and alpha 1I3 rose 2-fold from 4.4 to 10.7 micrograms UMP inhibited. The increases in the tissue inhibitor are interpreted
to be due to increased synthesis by the tissue, whereas the changes in alpha-macroglobulins are postulated to be due to increased
vascularity and increased permeability of the vessels.</abstract><cop>United States</cop><pub>Society for the Study of Reproduction</pub><pmid>1723897</pmid><doi>10.1095/biolreprod45.2.334</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-3363 |
| ispartof | Biology of reproduction, 1991-08, Vol.45 (2), p.334-342 |
| issn | 0006-3363 1529-7268 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72656380 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | alpha-Macroglobulins - isolation & purification alpha-Macroglobulins - physiology Animals Collagen - metabolism Extracellular Matrix - metabolism Female Glycoproteins - isolation & purification Glycoproteins - physiology Hot Temperature Metalloendopeptidases - antagonists & inhibitors Ovary - chemistry Ovulation Protein Denaturation Rats Rats, Inbred Strains Tissue Inhibitor of Metalloproteinases |
| title | A tissue inhibitor of metalloproteinases and alpha-macroglobulins in the ovulating rat ovary: possible regulators of collagen matrix breakdown |
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