Cochlear mitochondrial DNA3867bp deletion in aged mice
To study the status of cochlear mitochondrial DNA (mtDNA) and to determine the location of mtDNA deletion in aged mice. We detected cochlear mtDNA in 2, 7 - 10 and 17 - 19 month old mice by nested polymerase chain reaction (PCR) and DNA sequencing. mtDNA3867bp deletions were found in the cochleae of...
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| Veröffentlicht in: | Chinese medical journal 2002-09, Vol.115 (9), p.1390-1393 |
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| container_title | Chinese medical journal |
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| creator | Zhang, Xinxin Han, Dongyi Ding, Dalian Dai, Pu Yang, Weiyan Jiang, Sichang Salvi, Richard J |
| description | To study the status of cochlear mitochondrial DNA (mtDNA) and to determine the location of mtDNA deletion in aged mice.
We detected cochlear mtDNA in 2, 7 - 10 and 17 - 19 month old mice by nested polymerase chain reaction (PCR) and DNA sequencing.
mtDNA3867bp deletions were found in the cochleae of aged mice. The deletion occurred within nt9103-nt12970 and were flanked by 15 base pair direct repeats. Comparing the incidence of mtDNA3867bp deletions, 17 - 19 month old mice (7/8) were significantly higher than 7 - 10 month old mice (4/16). The deletion was not observed in 2 month old mice (0/7). The ratio of deleted mtDNA/total mtDNA in 17 - 19 month old mice was higher than in 7 - 10 month old mice (P < 0.001).
Cochlear mtDNA 3867bp deletion in aged mice may be related to presbycusis. |
| format | Article |
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We detected cochlear mtDNA in 2, 7 - 10 and 17 - 19 month old mice by nested polymerase chain reaction (PCR) and DNA sequencing.
mtDNA3867bp deletions were found in the cochleae of aged mice. The deletion occurred within nt9103-nt12970 and were flanked by 15 base pair direct repeats. Comparing the incidence of mtDNA3867bp deletions, 17 - 19 month old mice (7/8) were significantly higher than 7 - 10 month old mice (4/16). The deletion was not observed in 2 month old mice (0/7). The ratio of deleted mtDNA/total mtDNA in 17 - 19 month old mice was higher than in 7 - 10 month old mice (P < 0.001).
Cochlear mtDNA 3867bp deletion in aged mice may be related to presbycusis.</description><identifier>ISSN: 0366-6999</identifier><identifier>PMID: 12411120</identifier><language>eng</language><publisher>China</publisher><subject>Aging - genetics ; Animals ; Base Sequence ; Cochlea - metabolism ; DNA, Mitochondrial - analysis ; DNA, Mitochondrial - genetics ; Mice ; Molecular Sequence Data ; Oxidative Phosphorylation ; Presbycusis - etiology ; Sequence Deletion</subject><ispartof>Chinese medical journal, 2002-09, Vol.115 (9), p.1390-1393</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12411120$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Xinxin</creatorcontrib><creatorcontrib>Han, Dongyi</creatorcontrib><creatorcontrib>Ding, Dalian</creatorcontrib><creatorcontrib>Dai, Pu</creatorcontrib><creatorcontrib>Yang, Weiyan</creatorcontrib><creatorcontrib>Jiang, Sichang</creatorcontrib><creatorcontrib>Salvi, Richard J</creatorcontrib><title>Cochlear mitochondrial DNA3867bp deletion in aged mice</title><title>Chinese medical journal</title><addtitle>Chin Med J (Engl)</addtitle><description>To study the status of cochlear mitochondrial DNA (mtDNA) and to determine the location of mtDNA deletion in aged mice.
We detected cochlear mtDNA in 2, 7 - 10 and 17 - 19 month old mice by nested polymerase chain reaction (PCR) and DNA sequencing.
mtDNA3867bp deletions were found in the cochleae of aged mice. The deletion occurred within nt9103-nt12970 and were flanked by 15 base pair direct repeats. Comparing the incidence of mtDNA3867bp deletions, 17 - 19 month old mice (7/8) were significantly higher than 7 - 10 month old mice (4/16). The deletion was not observed in 2 month old mice (0/7). The ratio of deleted mtDNA/total mtDNA in 17 - 19 month old mice was higher than in 7 - 10 month old mice (P < 0.001).
Cochlear mtDNA 3867bp deletion in aged mice may be related to presbycusis.</description><subject>Aging - genetics</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Cochlea - metabolism</subject><subject>DNA, Mitochondrial - analysis</subject><subject>DNA, Mitochondrial - genetics</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Oxidative Phosphorylation</subject><subject>Presbycusis - etiology</subject><subject>Sequence Deletion</subject><issn>0366-6999</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1zzlPAzEUBGAXIBICfwFtRbeSj_gqo-WUImigXtnPb8HIe2DvFvx7gkiqmeLTSHNG1lQoVStr7YpclvJFKZdSqwuyYnzLGON0TVQzwmdCl6s-zoc6DiFHl6q7l50wSvupCphwjuNQxaFyHxgOEPCKnHcuFbw-5oa8P9y_NU_1_vXxudnt64lTPdfM2wCeSkCgDDnrhFQOTADJtEAmreLoZQfSdoBua7QPwoMyjFlQzhixIbf_u1Mevxcsc9vHApiSG3BcSqu5ksKIP3hzhIvvMbRTjr3LP-3pqfgFRDVNSQ</recordid><startdate>200209</startdate><enddate>200209</enddate><creator>Zhang, Xinxin</creator><creator>Han, Dongyi</creator><creator>Ding, Dalian</creator><creator>Dai, Pu</creator><creator>Yang, Weiyan</creator><creator>Jiang, Sichang</creator><creator>Salvi, Richard J</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200209</creationdate><title>Cochlear mitochondrial DNA3867bp deletion in aged mice</title><author>Zhang, Xinxin ; Han, Dongyi ; Ding, Dalian ; Dai, Pu ; Yang, Weiyan ; Jiang, Sichang ; Salvi, Richard J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p207t-1b9dcb05cec01e21f356ac8dc5173e15962eb5fc59fcea487bd3bc68119c6a883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Aging - genetics</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Cochlea - metabolism</topic><topic>DNA, Mitochondrial - analysis</topic><topic>DNA, Mitochondrial - genetics</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Oxidative Phosphorylation</topic><topic>Presbycusis - etiology</topic><topic>Sequence Deletion</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Xinxin</creatorcontrib><creatorcontrib>Han, Dongyi</creatorcontrib><creatorcontrib>Ding, Dalian</creatorcontrib><creatorcontrib>Dai, Pu</creatorcontrib><creatorcontrib>Yang, Weiyan</creatorcontrib><creatorcontrib>Jiang, Sichang</creatorcontrib><creatorcontrib>Salvi, Richard J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Chinese medical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Xinxin</au><au>Han, Dongyi</au><au>Ding, Dalian</au><au>Dai, Pu</au><au>Yang, Weiyan</au><au>Jiang, Sichang</au><au>Salvi, Richard J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cochlear mitochondrial DNA3867bp deletion in aged mice</atitle><jtitle>Chinese medical journal</jtitle><addtitle>Chin Med J (Engl)</addtitle><date>2002-09</date><risdate>2002</risdate><volume>115</volume><issue>9</issue><spage>1390</spage><epage>1393</epage><pages>1390-1393</pages><issn>0366-6999</issn><abstract>To study the status of cochlear mitochondrial DNA (mtDNA) and to determine the location of mtDNA deletion in aged mice.
We detected cochlear mtDNA in 2, 7 - 10 and 17 - 19 month old mice by nested polymerase chain reaction (PCR) and DNA sequencing.
mtDNA3867bp deletions were found in the cochleae of aged mice. The deletion occurred within nt9103-nt12970 and were flanked by 15 base pair direct repeats. Comparing the incidence of mtDNA3867bp deletions, 17 - 19 month old mice (7/8) were significantly higher than 7 - 10 month old mice (4/16). The deletion was not observed in 2 month old mice (0/7). The ratio of deleted mtDNA/total mtDNA in 17 - 19 month old mice was higher than in 7 - 10 month old mice (P < 0.001).
Cochlear mtDNA 3867bp deletion in aged mice may be related to presbycusis.</abstract><cop>China</cop><pmid>12411120</pmid><tpages>4</tpages></addata></record> |
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| ispartof | Chinese medical journal, 2002-09, Vol.115 (9), p.1390-1393 |
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| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Aging - genetics Animals Base Sequence Cochlea - metabolism DNA, Mitochondrial - analysis DNA, Mitochondrial - genetics Mice Molecular Sequence Data Oxidative Phosphorylation Presbycusis - etiology Sequence Deletion |
| title | Cochlear mitochondrial DNA3867bp deletion in aged mice |
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