Ultrafast Papanicolaou stain and cell-transfer technique enhance cytologic diagnosis of Hodgkin lymphoma
Diagnosis of Hodgkin lymphoma (HL) by fine‐needle aspiration (FNA) is often hampered by aspirated blood that camouflage scattered Hodgkin cells and Reed‐Sternberg (HRS) cells, and the absence of HRS cells in the smears submitted for immunophenotyping. The objective of this study was to develop a sim...
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| Veröffentlicht in: | Diagnostic cytopathology 2002-11, Vol.27 (5), p.308-311 |
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| creator | Zu, Youli Gangi, Maryann D. Yang, Grace C.H. |
| description | Diagnosis of Hodgkin lymphoma (HL) by fine‐needle aspiration (FNA) is often hampered by aspirated blood that camouflage scattered Hodgkin cells and Reed‐Sternberg (HRS) cells, and the absence of HRS cells in the smears submitted for immunophenotyping. The objective of this study was to develop a simple protocol to overcome these problems. The visibility of HRS cells in Diff‐Quik, traditional, and Ultrafast Papanicolaou (UFP) stains in FNA smears were compared in 73 cases of HL. HRS cells were found to be most visible in UFP because of the hemolysis of aspirated blood and the highlighting of HRS cells by the red‐staining nucleoli. UFP‐stained smears containing HRS cells were subsequently immunophenotyped via the cell‐transfer technique. UFP staining was found to have no deleterious effect on the immunoreactivity of cellular CD15 and CD30 antigens of HRS cells. This simple protocol enhances the cytologic diagnosis of HL, feasible even with a single smear. Diagn. Cytopathol. 2002;27:308–311. © 2002 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/dc.10180 |
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The objective of this study was to develop a simple protocol to overcome these problems. The visibility of HRS cells in Diff‐Quik, traditional, and Ultrafast Papanicolaou (UFP) stains in FNA smears were compared in 73 cases of HL. HRS cells were found to be most visible in UFP because of the hemolysis of aspirated blood and the highlighting of HRS cells by the red‐staining nucleoli. UFP‐stained smears containing HRS cells were subsequently immunophenotyped via the cell‐transfer technique. UFP staining was found to have no deleterious effect on the immunoreactivity of cellular CD15 and CD30 antigens of HRS cells. This simple protocol enhances the cytologic diagnosis of HL, feasible even with a single smear. Diagn. Cytopathol. 2002;27:308–311. © 2002 Wiley‐Liss, Inc.</description><identifier>ISSN: 8755-1039</identifier><identifier>EISSN: 1097-0339</identifier><identifier>DOI: 10.1002/dc.10180</identifier><identifier>PMID: 12411999</identifier><identifier>CODEN: DICYE7</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Azure Stains ; Biological and medical sciences ; Biopsy, Needle ; cell-transfer technique ; fine-needle aspiration biopsy ; Hematology ; Hodgkin Disease - diagnosis ; Hodgkin Disease - metabolism ; Hodgkin's disease ; Humans ; Immunohistochemistry ; Immunophenotyping ; Investigative techniques, diagnostic techniques (general aspects) ; Ki-1 Antigen - metabolism ; Lewis X Antigen - metabolism ; Medical sciences ; Methylene Blue ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Reed-Sternberg Cells - pathology ; Staining and Labeling - methods ; Xanthenes</subject><ispartof>Diagnostic cytopathology, 2002-11, Vol.27 (5), p.308-311</ispartof><rights>Copyright © 2002 Wiley‐Liss, Inc.</rights><rights>2003 INIST-CNRS</rights><rights>Copyright 2002 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4510-6669ed5d591670a958c2d67896a1c5dc249fa92e0579b08f698e66f07fc040643</citedby><cites>FETCH-LOGICAL-c4510-6669ed5d591670a958c2d67896a1c5dc249fa92e0579b08f698e66f07fc040643</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fdc.10180$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fdc.10180$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27911,27912,45561,45562</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14361668$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12411999$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zu, Youli</creatorcontrib><creatorcontrib>Gangi, Maryann D.</creatorcontrib><creatorcontrib>Yang, Grace C.H.</creatorcontrib><title>Ultrafast Papanicolaou stain and cell-transfer technique enhance cytologic diagnosis of Hodgkin lymphoma</title><title>Diagnostic cytopathology</title><addtitle>Diagn. Cytopathol</addtitle><description>Diagnosis of Hodgkin lymphoma (HL) by fine‐needle aspiration (FNA) is often hampered by aspirated blood that camouflage scattered Hodgkin cells and Reed‐Sternberg (HRS) cells, and the absence of HRS cells in the smears submitted for immunophenotyping. The objective of this study was to develop a simple protocol to overcome these problems. The visibility of HRS cells in Diff‐Quik, traditional, and Ultrafast Papanicolaou (UFP) stains in FNA smears were compared in 73 cases of HL. HRS cells were found to be most visible in UFP because of the hemolysis of aspirated blood and the highlighting of HRS cells by the red‐staining nucleoli. UFP‐stained smears containing HRS cells were subsequently immunophenotyped via the cell‐transfer technique. UFP staining was found to have no deleterious effect on the immunoreactivity of cellular CD15 and CD30 antigens of HRS cells. This simple protocol enhances the cytologic diagnosis of HL, feasible even with a single smear. Diagn. Cytopathol. 2002;27:308–311. © 2002 Wiley‐Liss, Inc.</description><subject>Azure Stains</subject><subject>Biological and medical sciences</subject><subject>Biopsy, Needle</subject><subject>cell-transfer technique</subject><subject>fine-needle aspiration biopsy</subject><subject>Hematology</subject><subject>Hodgkin Disease - diagnosis</subject><subject>Hodgkin Disease - metabolism</subject><subject>Hodgkin's disease</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Immunophenotyping</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Ki-1 Antigen - metabolism</subject><subject>Lewis X Antigen - metabolism</subject><subject>Medical sciences</subject><subject>Methylene Blue</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Reed-Sternberg Cells - pathology</subject><subject>Staining and Labeling - methods</subject><subject>Xanthenes</subject><issn>8755-1039</issn><issn>1097-0339</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10E1vEzEQBmALUdFQkPgFyBcQl4Wxd22vjyiUFKmCHKg4WlN_JKbedVhvVPLvuyELPXGay6N3Zl5CXjF4zwD4B2enyVp4QhYMtKqgrvVTsmiVEBWDWp-T56X8BADNmXxGzhlvGNNaL8j2Jo0DBiwjXeMO-2hzwrynZcTYU-wdtT6lajJ9CX6go7fbPv7ae-r7LfbWU3sYc8qbaKmLuOlziYXmQK-y29xNEenQ7ba5wxfkLGAq_uU8L8jN58vvy6vq-tvqy_LjdWUbwaCSUmrvhBOaSQWoRWu5k6rVEpkVzvJGB9Tcg1D6FtogdeulDKCChQZkU1-Qt6fc3ZCnM8touliOP2Dv874YxaXgCo7w3QnaIZcy-GB2Q-xwOBgG5tiqcdb8aXWir-fM_W3n3SOca5zAmxlgsZjC1JaN5dE1tWRStpOrTu4-Jn_470Lzafl38exjGf3vfx6HOyNVrYT58XVltFit1zVfm6Z-AKG_nCA</recordid><startdate>200211</startdate><enddate>200211</enddate><creator>Zu, Youli</creator><creator>Gangi, Maryann D.</creator><creator>Yang, Grace C.H.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200211</creationdate><title>Ultrafast Papanicolaou stain and cell-transfer technique enhance cytologic diagnosis of Hodgkin lymphoma</title><author>Zu, Youli ; Gangi, Maryann D. ; Yang, Grace C.H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4510-6669ed5d591670a958c2d67896a1c5dc249fa92e0579b08f698e66f07fc040643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Azure Stains</topic><topic>Biological and medical sciences</topic><topic>Biopsy, Needle</topic><topic>cell-transfer technique</topic><topic>fine-needle aspiration biopsy</topic><topic>Hematology</topic><topic>Hodgkin Disease - diagnosis</topic><topic>Hodgkin Disease - metabolism</topic><topic>Hodgkin's disease</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Immunophenotyping</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Ki-1 Antigen - metabolism</topic><topic>Lewis X Antigen - metabolism</topic><topic>Medical sciences</topic><topic>Methylene Blue</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Reed-Sternberg Cells - pathology</topic><topic>Staining and Labeling - methods</topic><topic>Xanthenes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zu, Youli</creatorcontrib><creatorcontrib>Gangi, Maryann D.</creatorcontrib><creatorcontrib>Yang, Grace C.H.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Diagnostic cytopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zu, Youli</au><au>Gangi, Maryann D.</au><au>Yang, Grace C.H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultrafast Papanicolaou stain and cell-transfer technique enhance cytologic diagnosis of Hodgkin lymphoma</atitle><jtitle>Diagnostic cytopathology</jtitle><addtitle>Diagn. Cytopathol</addtitle><date>2002-11</date><risdate>2002</risdate><volume>27</volume><issue>5</issue><spage>308</spage><epage>311</epage><pages>308-311</pages><issn>8755-1039</issn><eissn>1097-0339</eissn><coden>DICYE7</coden><abstract>Diagnosis of Hodgkin lymphoma (HL) by fine‐needle aspiration (FNA) is often hampered by aspirated blood that camouflage scattered Hodgkin cells and Reed‐Sternberg (HRS) cells, and the absence of HRS cells in the smears submitted for immunophenotyping. The objective of this study was to develop a simple protocol to overcome these problems. The visibility of HRS cells in Diff‐Quik, traditional, and Ultrafast Papanicolaou (UFP) stains in FNA smears were compared in 73 cases of HL. HRS cells were found to be most visible in UFP because of the hemolysis of aspirated blood and the highlighting of HRS cells by the red‐staining nucleoli. UFP‐stained smears containing HRS cells were subsequently immunophenotyped via the cell‐transfer technique. UFP staining was found to have no deleterious effect on the immunoreactivity of cellular CD15 and CD30 antigens of HRS cells. This simple protocol enhances the cytologic diagnosis of HL, feasible even with a single smear. Diagn. Cytopathol. 2002;27:308–311. © 2002 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>12411999</pmid><doi>10.1002/dc.10180</doi><tpages>4</tpages></addata></record> |
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| subjects | Azure Stains Biological and medical sciences Biopsy, Needle cell-transfer technique fine-needle aspiration biopsy Hematology Hodgkin Disease - diagnosis Hodgkin Disease - metabolism Hodgkin's disease Humans Immunohistochemistry Immunophenotyping Investigative techniques, diagnostic techniques (general aspects) Ki-1 Antigen - metabolism Lewis X Antigen - metabolism Medical sciences Methylene Blue Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Reed-Sternberg Cells - pathology Staining and Labeling - methods Xanthenes |
| title | Ultrafast Papanicolaou stain and cell-transfer technique enhance cytologic diagnosis of Hodgkin lymphoma |
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