[32] Hepatocyte culture in study of P450 regulation
This chapter discusses the hepatocyte culture in study of P450 regulation. Primary cultures of adult rat hepatocytes provide a unique system for the study of many differentiated liver functions including induction of the cytochromes P450. These nonproliferating hepatocytes can be maintained in a che...
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| Veröffentlicht in: | Methods in Enzymology 1991, Vol.206, p.335-344 |
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| container_title | Methods in Enzymology |
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| creator | Li, Donna Schuetz, Erin G. Guzelian, Philip S. |
| description | This chapter discusses the hepatocyte culture in study of P450 regulation. Primary cultures of adult rat hepatocytes provide a unique system for the study of many differentiated liver functions including induction of the cytochromes P450. These nonproliferating hepatocytes can be maintained in a chemically defined, serum-free medium for weeks while retaining the capabilities of expressing a wide array of liver-specific functions. This chapter describes the essential elements in isolating hepatocytes with the use of proteolytic enzymes and culturing the viable cells. Adult male Sprague–Dawley rats weighing 200–250 g or females weighing 185–210 g can be purchased from commercial animal breeding sources. Rats are maintained in pairs in wire-bottomed cages with free access to chow diet and kept under regulated lighting. |
| doi_str_mv | 10.1016/0076-6879(91)06103-A |
| format | Article |
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Primary cultures of adult rat hepatocytes provide a unique system for the study of many differentiated liver functions including induction of the cytochromes P450. These nonproliferating hepatocytes can be maintained in a chemically defined, serum-free medium for weeks while retaining the capabilities of expressing a wide array of liver-specific functions. This chapter describes the essential elements in isolating hepatocytes with the use of proteolytic enzymes and culturing the viable cells. Adult male Sprague–Dawley rats weighing 200–250 g or females weighing 185–210 g can be purchased from commercial animal breeding sources. Rats are maintained in pairs in wire-bottomed cages with free access to chow diet and kept under regulated lighting.</description><identifier>ISSN: 0076-6879</identifier><identifier>ISBN: 0121821072</identifier><identifier>ISBN: 9780121821074</identifier><identifier>EISSN: 1557-7988</identifier><identifier>DOI: 10.1016/0076-6879(91)06103-A</identifier><identifier>PMID: 1784220</identifier><identifier>CODEN: MENZAU</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Science & Technology</publisher><subject>Animal cells ; Animals ; Biological and medical sciences ; Cell cultures. Hybridization. Fusion ; Cell Separation - instrumentation ; Cell Separation - methods ; Cells, Cultured ; Culture Media ; Culture Techniques - methods ; Cytochrome P-450 Enzyme System - metabolism ; Female ; Fundamental and applied biological sciences. 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Primary cultures of adult rat hepatocytes provide a unique system for the study of many differentiated liver functions including induction of the cytochromes P450. These nonproliferating hepatocytes can be maintained in a chemically defined, serum-free medium for weeks while retaining the capabilities of expressing a wide array of liver-specific functions. This chapter describes the essential elements in isolating hepatocytes with the use of proteolytic enzymes and culturing the viable cells. Adult male Sprague–Dawley rats weighing 200–250 g or females weighing 185–210 g can be purchased from commercial animal breeding sources. Rats are maintained in pairs in wire-bottomed cages with free access to chow diet and kept under regulated lighting.</description><subject>Animal cells</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell cultures. Hybridization. Fusion</subject><subject>Cell Separation - instrumentation</subject><subject>Cell Separation - methods</subject><subject>Cells, Cultured</subject><subject>Culture Media</subject><subject>Culture Techniques - methods</subject><subject>Cytochrome P-450 Enzyme System - metabolism</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Homeostasis</subject><subject>Indicators and Reagents</subject><subject>Liver - cytology</subject><subject>Liver - enzymology</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Molecular and cellular biology</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><issn>0076-6879</issn><issn>1557-7988</issn><isbn>0121821072</isbn><isbn>9780121821074</isbn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kUtLxDAUhYMPdBz9BwpdiOiiepM0r40wiC8Y0IWuREKa3Eqk045NK8y_t6ODd3MX5-NyzzmEHFO4pEDlFYCSudTKnBt6AZICz2dbZEKFULkyWm-TA6CMakZBsR0y-ef3yVFKnzBOYZgAs0f2qNIFYzAh_I2z9-wBl65v_arHzA91P3SYxSZL_RBWWVtlz4WArMOPoXZ9bJtDslu5OuHRZk_J693ty81DPn-6f7yZzXNkBvrceeYQfalKEyqQQQgA4QsNUnoGtKQBtTG6rBh1hkvNAzMFlYVSLGguKz4lZ393l137NWDq7SImj3XtGmyHZBWTAphmI3iyAYdygcEuu7hw3cpuXI766UZ3ybu66lzjY_rHxsek4GrErv8wHE19R-xs8hEbjyF26Hsb2mgp2HUZdh2uXYdrDbW_ZdgZ_wFdQXU9</recordid><startdate>1991</startdate><enddate>1991</enddate><creator>Li, Donna</creator><creator>Schuetz, Erin G.</creator><creator>Guzelian, Philip S.</creator><general>Elsevier Science & Technology</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>1991</creationdate><title>[32] Hepatocyte culture in study of P450 regulation</title><author>Li, Donna ; Schuetz, Erin G. ; Guzelian, Philip S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e290t-ac2aeecb7b9df06d55005c48066c201b1de8998bf21a93683d294164772d836f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animal cells</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell cultures. Hybridization. Fusion</topic><topic>Cell Separation - instrumentation</topic><topic>Cell Separation - methods</topic><topic>Cells, Cultured</topic><topic>Culture Media</topic><topic>Culture Techniques - methods</topic><topic>Cytochrome P-450 Enzyme System - metabolism</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Homeostasis</topic><topic>Indicators and Reagents</topic><topic>Liver - cytology</topic><topic>Liver - enzymology</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Molecular and cellular biology</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Donna</creatorcontrib><creatorcontrib>Schuetz, Erin G.</creatorcontrib><creatorcontrib>Guzelian, Philip S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Methods in Enzymology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Donna</au><au>Schuetz, Erin G.</au><au>Guzelian, Philip S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>[32] Hepatocyte culture in study of P450 regulation</atitle><jtitle>Methods in Enzymology</jtitle><addtitle>Methods Enzymol</addtitle><date>1991</date><risdate>1991</risdate><volume>206</volume><spage>335</spage><epage>344</epage><pages>335-344</pages><issn>0076-6879</issn><eissn>1557-7988</eissn><isbn>0121821072</isbn><isbn>9780121821074</isbn><coden>MENZAU</coden><abstract>This chapter discusses the hepatocyte culture in study of P450 regulation. 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| ispartof | Methods in Enzymology, 1991, Vol.206, p.335-344 |
| issn | 0076-6879 1557-7988 |
| language | eng |
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| source | MEDLINE; ScienceDirect eBooks; Access via ScienceDirect (Elsevier) |
| subjects | Animal cells Animals Biological and medical sciences Cell cultures. Hybridization. Fusion Cell Separation - instrumentation Cell Separation - methods Cells, Cultured Culture Media Culture Techniques - methods Cytochrome P-450 Enzyme System - metabolism Female Fundamental and applied biological sciences. Psychology Homeostasis Indicators and Reagents Liver - cytology Liver - enzymology Male Mice Mice, Inbred C57BL Molecular and cellular biology Rats Rats, Inbred Strains |
| title | [32] Hepatocyte culture in study of P450 regulation |
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