Abundance of the RSC nucleosome-remodeling complex is important for the cells to tolerate DNA damage in Saccharomyces cerevisiae
The essential Nps1p/Sth1p is a catalytic subunit of the nucleosome-remodeling complex, RSC, of Saccharomyces cerevisiae that can alter nucleosome structure by using the energy of ATP hydrolysis. Besides the ATPase domain, Nps1p harbors the bromodomain, of which the function(s) have not yet been defi...
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| Veröffentlicht in: | FEBS letters 2002-11, Vol.531 (2), p.215-221 |
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| creator | Koyama, Hirofumi Itoh, Masayuki Miyahara, Kohji Tsuchiya, Eiko |
| description | The essential Nps1p/Sth1p is a catalytic subunit of the nucleosome-remodeling complex, RSC, of
Saccharomyces cerevisiae that can alter nucleosome structure by using the energy of ATP hydrolysis. Besides the ATPase domain, Nps1p harbors the bromodomain, of which the function(s) have not yet been defined. We have isolated a temperature-sensitive mutant allele of
NPS1,
nps1-13, which has amino acid substitutions within the bromodomain. This mutation perturbed the interaction between the RSC components and enhanced the sensitivity of the cells to several DNA-damaging treatments at the permissive temperature. Reduced expression of
NPS1 also caused DNA damage sensitivity. These results suggest the importance of the Nps1p bromodomain in RSC integrity and a model in which high amounts of RSC would be required for the cells to overcome DNA damage. |
| doi_str_mv | 10.1016/S0014-5793(02)03504-4 |
| format | Article |
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Saccharomyces cerevisiae that can alter nucleosome structure by using the energy of ATP hydrolysis. Besides the ATPase domain, Nps1p harbors the bromodomain, of which the function(s) have not yet been defined. We have isolated a temperature-sensitive mutant allele of
NPS1,
nps1-13, which has amino acid substitutions within the bromodomain. This mutation perturbed the interaction between the RSC components and enhanced the sensitivity of the cells to several DNA-damaging treatments at the permissive temperature. Reduced expression of
NPS1 also caused DNA damage sensitivity. These results suggest the importance of the Nps1p bromodomain in RSC integrity and a model in which high amounts of RSC would be required for the cells to overcome DNA damage.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/S0014-5793(02)03504-4</identifier><identifier>PMID: 12417315</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Bromodomain ; Cell Cycle Proteins - chemistry ; Cell Cycle Proteins - genetics ; Cell Cycle Proteins - physiology ; Conserved Sequence ; DNA Damage ; DNA, Fungal - analysis ; Fungal Proteins - chemistry ; Fungal Proteins - genetics ; Fungal Proteins - physiology ; Macromolecular Substances ; Mutation ; NPS1/ STH1 ; Nuclear Proteins ; Nucleosome-remodeling complex ; Nucleosomes - physiology ; Phenotype ; Protein Structure, Tertiary ; Protein Subunits ; RSC ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae Proteins ; Temperature</subject><ispartof>FEBS letters, 2002-11, Vol.531 (2), p.215-221</ispartof><rights>2002 Federation of European Biochemical Societies</rights><rights>FEBS Letters 531 (2002) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5704-228c22c797194a6e88878dfa3223f71152b7082b3b736e8bf09ec9da7d9613873</citedby><cites>FETCH-LOGICAL-c5704-228c22c797194a6e88878dfa3223f71152b7082b3b736e8bf09ec9da7d9613873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0014-5793%2802%2903504-4$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0014-5793(02)03504-4$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,1416,1432,3548,27923,27924,45573,45574,45994,46408,46832</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12417315$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koyama, Hirofumi</creatorcontrib><creatorcontrib>Itoh, Masayuki</creatorcontrib><creatorcontrib>Miyahara, Kohji</creatorcontrib><creatorcontrib>Tsuchiya, Eiko</creatorcontrib><title>Abundance of the RSC nucleosome-remodeling complex is important for the cells to tolerate DNA damage in Saccharomyces cerevisiae</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>The essential Nps1p/Sth1p is a catalytic subunit of the nucleosome-remodeling complex, RSC, of
Saccharomyces cerevisiae that can alter nucleosome structure by using the energy of ATP hydrolysis. Besides the ATPase domain, Nps1p harbors the bromodomain, of which the function(s) have not yet been defined. We have isolated a temperature-sensitive mutant allele of
NPS1,
nps1-13, which has amino acid substitutions within the bromodomain. This mutation perturbed the interaction between the RSC components and enhanced the sensitivity of the cells to several DNA-damaging treatments at the permissive temperature. Reduced expression of
NPS1 also caused DNA damage sensitivity. These results suggest the importance of the Nps1p bromodomain in RSC integrity and a model in which high amounts of RSC would be required for the cells to overcome DNA damage.</description><subject>Bromodomain</subject><subject>Cell Cycle Proteins - chemistry</subject><subject>Cell Cycle Proteins - genetics</subject><subject>Cell Cycle Proteins - physiology</subject><subject>Conserved Sequence</subject><subject>DNA Damage</subject><subject>DNA, Fungal - analysis</subject><subject>Fungal Proteins - chemistry</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - physiology</subject><subject>Macromolecular Substances</subject><subject>Mutation</subject><subject>NPS1/ STH1</subject><subject>Nuclear Proteins</subject><subject>Nucleosome-remodeling complex</subject><subject>Nucleosomes - physiology</subject><subject>Phenotype</subject><subject>Protein Structure, Tertiary</subject><subject>Protein Subunits</subject><subject>RSC</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae Proteins</subject><subject>Temperature</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi0EokvhJ4B8QvQQ8EcSOye0bFuKVIHEwtly7ElrFMdbOynsjZ-Ok13BsUiWLM888854XoReUvKWElq_2xJCy6ISDX9D2BnhFSmL8hFaUSl4wctaPkarv8gJepbSD5LfkjZP0QllJRWcViv0e91Og9WDARw6PN4C_rrd4GEyPYQUPBQRfLDQu-EGm-B3PfzCLmHndyGOehhxF-JSZqDvEx5DPj1EPQI-_7zGVnt9A9gNeKuNudUx-L2BlOkI9y45Dc_Rk073CV4c71P0_fLi2-aquP7y8dNmfV2YSuSvMSYNY0Y0gjalrkFKKaTtNGeMd4LSirWCSNbyVvCcbTvSgGmsFrapKc87OUWvD7q7GO4mSKPyLs1D6wHClJRgddmw8mEwL5iympEMVgfQxJBShE7tovM67hUlavZILR6p2QBFmFo8UmWue3VsMLUe7L-qoykZuDoAP10P-_9TVZcXH9iSmROELeG51_uDFOTV3juIKhkH2W3rIphR2eAemPYPnOa0oA</recordid><startdate>20021106</startdate><enddate>20021106</enddate><creator>Koyama, Hirofumi</creator><creator>Itoh, Masayuki</creator><creator>Miyahara, Kohji</creator><creator>Tsuchiya, Eiko</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20021106</creationdate><title>Abundance of the RSC nucleosome-remodeling complex is important for the cells to tolerate DNA damage in Saccharomyces cerevisiae</title><author>Koyama, Hirofumi ; Itoh, Masayuki ; Miyahara, Kohji ; Tsuchiya, Eiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5704-228c22c797194a6e88878dfa3223f71152b7082b3b736e8bf09ec9da7d9613873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Bromodomain</topic><topic>Cell Cycle Proteins - chemistry</topic><topic>Cell Cycle Proteins - genetics</topic><topic>Cell Cycle Proteins - physiology</topic><topic>Conserved Sequence</topic><topic>DNA Damage</topic><topic>DNA, Fungal - analysis</topic><topic>Fungal Proteins - chemistry</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - physiology</topic><topic>Macromolecular Substances</topic><topic>Mutation</topic><topic>NPS1/ STH1</topic><topic>Nuclear Proteins</topic><topic>Nucleosome-remodeling complex</topic><topic>Nucleosomes - physiology</topic><topic>Phenotype</topic><topic>Protein Structure, Tertiary</topic><topic>Protein Subunits</topic><topic>RSC</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Saccharomyces cerevisiae Proteins</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koyama, Hirofumi</creatorcontrib><creatorcontrib>Itoh, Masayuki</creatorcontrib><creatorcontrib>Miyahara, Kohji</creatorcontrib><creatorcontrib>Tsuchiya, Eiko</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koyama, Hirofumi</au><au>Itoh, Masayuki</au><au>Miyahara, Kohji</au><au>Tsuchiya, Eiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Abundance of the RSC nucleosome-remodeling complex is important for the cells to tolerate DNA damage in Saccharomyces cerevisiae</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2002-11-06</date><risdate>2002</risdate><volume>531</volume><issue>2</issue><spage>215</spage><epage>221</epage><pages>215-221</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>The essential Nps1p/Sth1p is a catalytic subunit of the nucleosome-remodeling complex, RSC, of
Saccharomyces cerevisiae that can alter nucleosome structure by using the energy of ATP hydrolysis. Besides the ATPase domain, Nps1p harbors the bromodomain, of which the function(s) have not yet been defined. We have isolated a temperature-sensitive mutant allele of
NPS1,
nps1-13, which has amino acid substitutions within the bromodomain. This mutation perturbed the interaction between the RSC components and enhanced the sensitivity of the cells to several DNA-damaging treatments at the permissive temperature. Reduced expression of
NPS1 also caused DNA damage sensitivity. These results suggest the importance of the Nps1p bromodomain in RSC integrity and a model in which high amounts of RSC would be required for the cells to overcome DNA damage.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>12417315</pmid><doi>10.1016/S0014-5793(02)03504-4</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals; Elsevier ScienceDirect Journals Complete; Wiley Free Archive; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Bromodomain Cell Cycle Proteins - chemistry Cell Cycle Proteins - genetics Cell Cycle Proteins - physiology Conserved Sequence DNA Damage DNA, Fungal - analysis Fungal Proteins - chemistry Fungal Proteins - genetics Fungal Proteins - physiology Macromolecular Substances Mutation NPS1/ STH1 Nuclear Proteins Nucleosome-remodeling complex Nucleosomes - physiology Phenotype Protein Structure, Tertiary Protein Subunits RSC Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins Temperature |
| title | Abundance of the RSC nucleosome-remodeling complex is important for the cells to tolerate DNA damage in Saccharomyces cerevisiae |
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