Evaluation of different detection methods of biofilm formation in Staphylococcus aureus
The icaADBC gene locus of Staphylococcus aureus and its polysaccharide intercellular adhesin (PIA/PNSG) were recently identified, but biofilm formation has rarely been detected in vitro. In this study we evaluated a tissue culture plate (TCP) assay and a tube test, as well as Congo red agar, using t...
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| Veröffentlicht in: | Medical microbiology and immunology 2002-10, Vol.191 (2), p.101-106 |
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| creator | KNOBLOCH, Johannes K.-M HORSTKOTTE, Matthias A ROHDE, Holger MACK, Dietrich |
| description | The icaADBC gene locus of Staphylococcus aureus and its polysaccharide intercellular adhesin (PIA/PNSG) were recently identified, but biofilm formation has rarely been detected in vitro. In this study we evaluated a tissue culture plate (TCP) assay and a tube test, as well as Congo red agar, using the two basic media trypticase soy broth (TSB) and brain heart infusion (BHI) broth with different sugar supplements for detection of biofilm formation in 128 ica-positive S. aureus isolates. Of the S. aureus strains, 57.1% displayed a biofilm-positive phenotype under optimized conditions in the TCP test. The tube test correlated well with the TCP test for strongly biofilm-producing strains, whereas weak producers were not safely discriminated from biofilm-negative strains. Screening on Congo red agar displayed a strong correlation with the TCP and the tube test for only 3.8%, and is therefore not recommended for investigation of biofilm formation in S. aureus. |
| doi_str_mv | 10.1007/s00430-002-0124-3 |
| format | Article |
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In this study we evaluated a tissue culture plate (TCP) assay and a tube test, as well as Congo red agar, using the two basic media trypticase soy broth (TSB) and brain heart infusion (BHI) broth with different sugar supplements for detection of biofilm formation in 128 ica-positive S. aureus isolates. Of the S. aureus strains, 57.1% displayed a biofilm-positive phenotype under optimized conditions in the TCP test. The tube test correlated well with the TCP test for strongly biofilm-producing strains, whereas weak producers were not safely discriminated from biofilm-negative strains. Screening on Congo red agar displayed a strong correlation with the TCP and the tube test for only 3.8%, and is therefore not recommended for investigation of biofilm formation in S. aureus.</description><identifier>ISSN: 0300-8584</identifier><identifier>EISSN: 1432-1831</identifier><identifier>DOI: 10.1007/s00430-002-0124-3</identifier><identifier>PMID: 12410349</identifier><identifier>CODEN: MMIYAO</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Agar - metabolism ; Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Biofilms - growth & development ; Biological and medical sciences ; Caseins ; Congo Red - metabolism ; Culture Media - classification ; Culture Techniques - methods ; Fundamental and applied biological sciences. 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In this study we evaluated a tissue culture plate (TCP) assay and a tube test, as well as Congo red agar, using the two basic media trypticase soy broth (TSB) and brain heart infusion (BHI) broth with different sugar supplements for detection of biofilm formation in 128 ica-positive S. aureus isolates. Of the S. aureus strains, 57.1% displayed a biofilm-positive phenotype under optimized conditions in the TCP test. The tube test correlated well with the TCP test for strongly biofilm-producing strains, whereas weak producers were not safely discriminated from biofilm-negative strains. Screening on Congo red agar displayed a strong correlation with the TCP and the tube test for only 3.8%, and is therefore not recommended for investigation of biofilm formation in S. aureus.</description><subject>Agar - metabolism</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biofilms - growth & development</subject><subject>Biological and medical sciences</subject><subject>Caseins</subject><subject>Congo Red - metabolism</subject><subject>Culture Media - classification</subject><subject>Culture Techniques - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene loci</subject><subject>Immunology</subject><subject>Infections</subject><subject>Microbiology</subject><subject>N-Acetylglucosaminyltransferases</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Protein Hydrolysates</subject><subject>Staphylococcus aureus - genetics</subject><subject>Staphylococcus aureus - isolation & purification</subject><subject>Staphylococcus aureus - physiology</subject><subject>Time Factors</subject><issn>0300-8584</issn><issn>1432-1831</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkU1LxDAQhoMo7rr6A7xIEfRWnWSSNj3K4hcseFDxGNI0wUrbrEkr-O_t2oUFL54GZp73heEh5JTCFQXIryMAR0gBWAqU8RT3yJxyZCmVSPfJHBAglULyGTmK8QOA5hmDQzIbYQrIizl5u_3SzaD72neJd0lVO2eD7fqksr01v-vW9u--iptzWXtXN23ifGinTN0lz71ev3833nhjhpjoIdghHpMDp5toT7ZzQV7vbl-WD-nq6f5xebNKDUrRp9aVAoQRmFfcoagAM1fIErmlJrPcaVExWVhBc8YQRGnKwoEsZZEhSJbnuCCXU-86-M_Bxl61dTS2aXRn_RBVzjKOhcj-BanMIOcMRvD8D_jhh9CNTyjGgKKgUowQnSATfIzBOrUOdavDt6KgNm7U5EaNbtTGjcIxc7YtHsrWVrvEVsYIXGwBHY1uXNCdqeOO46NVKRF_AHmzleI</recordid><startdate>20021001</startdate><enddate>20021001</enddate><creator>KNOBLOCH, Johannes K.-M</creator><creator>HORSTKOTTE, Matthias A</creator><creator>ROHDE, Holger</creator><creator>MACK, Dietrich</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20021001</creationdate><title>Evaluation of different detection methods of biofilm formation in Staphylococcus aureus</title><author>KNOBLOCH, Johannes K.-M ; HORSTKOTTE, Matthias A ; ROHDE, Holger ; MACK, Dietrich</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-efb505c537d4f35d036f98b34e1c6e4fa5d289e51722305bcb9f08b8963082773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Agar - metabolism</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Biofilms - growth & development</topic><topic>Biological and medical sciences</topic><topic>Caseins</topic><topic>Congo Red - metabolism</topic><topic>Culture Media - classification</topic><topic>Culture Techniques - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene loci</topic><topic>Immunology</topic><topic>Infections</topic><topic>Microbiology</topic><topic>N-Acetylglucosaminyltransferases</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Protein Hydrolysates</topic><topic>Staphylococcus aureus - genetics</topic><topic>Staphylococcus aureus - isolation & purification</topic><topic>Staphylococcus aureus - physiology</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KNOBLOCH, Johannes K.-M</creatorcontrib><creatorcontrib>HORSTKOTTE, Matthias A</creatorcontrib><creatorcontrib>ROHDE, Holger</creatorcontrib><creatorcontrib>MACK, Dietrich</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Medical microbiology and immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KNOBLOCH, Johannes K.-M</au><au>HORSTKOTTE, Matthias A</au><au>ROHDE, Holger</au><au>MACK, Dietrich</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of different detection methods of biofilm formation in Staphylococcus aureus</atitle><jtitle>Medical microbiology and immunology</jtitle><addtitle>Med Microbiol Immunol</addtitle><date>2002-10-01</date><risdate>2002</risdate><volume>191</volume><issue>2</issue><spage>101</spage><epage>106</epage><pages>101-106</pages><issn>0300-8584</issn><eissn>1432-1831</eissn><coden>MMIYAO</coden><abstract>The icaADBC gene locus of Staphylococcus aureus and its polysaccharide intercellular adhesin (PIA/PNSG) were recently identified, but biofilm formation has rarely been detected in vitro. In this study we evaluated a tissue culture plate (TCP) assay and a tube test, as well as Congo red agar, using the two basic media trypticase soy broth (TSB) and brain heart infusion (BHI) broth with different sugar supplements for detection of biofilm formation in 128 ica-positive S. aureus isolates. Of the S. aureus strains, 57.1% displayed a biofilm-positive phenotype under optimized conditions in the TCP test. The tube test correlated well with the TCP test for strongly biofilm-producing strains, whereas weak producers were not safely discriminated from biofilm-negative strains. Screening on Congo red agar displayed a strong correlation with the TCP and the tube test for only 3.8%, and is therefore not recommended for investigation of biofilm formation in S. aureus.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>12410349</pmid><doi>10.1007/s00430-002-0124-3</doi><tpages>6</tpages></addata></record> |
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| ispartof | Medical microbiology and immunology, 2002-10, Vol.191 (2), p.101-106 |
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| subjects | Agar - metabolism Bacteriological methods and techniques used in bacteriology Bacteriology Biofilms - growth & development Biological and medical sciences Caseins Congo Red - metabolism Culture Media - classification Culture Techniques - methods Fundamental and applied biological sciences. Psychology Gene loci Immunology Infections Microbiology N-Acetylglucosaminyltransferases Polymerase Chain Reaction - methods Protein Hydrolysates Staphylococcus aureus - genetics Staphylococcus aureus - isolation & purification Staphylococcus aureus - physiology Time Factors |
| title | Evaluation of different detection methods of biofilm formation in Staphylococcus aureus |
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