Characterization and functional expression of a cDNA encoding egasyn (esterase-22): the endoplasmic reticulum-targeting protein of β-glucuronidase
Egasyn (esterase-22), a member of the nonspecific carboxylesterase multigene family (E.C. 3.1.1.1.), is the endoplasmic reticulum (ER)-targeting protein of β-glucuronidase. We utilized the polymerase chain reaction (PCR) in the eventual isolation of murine egasyn cDNAs. PCR primers were based upon:...
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| Veröffentlicht in: | Genomics (San Diego, Calif.) Calif.), 1991-12, Vol.11 (4), p.956-967 |
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| container_title | Genomics (San Diego, Calif.) |
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| creator | Ovnic, Mariana Swank, Richard T. Fletcher, Colin Zhen, Lida Novak, Edward K. Baumann, Heinz Heintz, Nathanial Ganschow, Roger E. |
| description | Egasyn (esterase-22), a member of the nonspecific carboxylesterase multigene family (E.C. 3.1.1.1.), is the endoplasmic reticulum (ER)-targeting protein of β-glucuronidase. We utilized the polymerase chain reaction (PCR) in the eventual isolation of murine egasyn cDNAs. PCR primers were based upon: (1) partial amino acid sequences derived from egasyn peptides and (2) a conserved active site region shared by carboxylesterases. The amino acid sequence deduced from the PCR product matched that obtained from egasyn protein. This product was utilized as a probe to screen a cDNA library. Two cDNAs whose composite sequence encoded an open reading frame of 562 amino acids were isolated. A message size of 1700–2000 bp was revealed by RNA blot hybridization analysis. S1 nuclease protection analyses detected mRNA in liver, kidney, lung, and submandibular gland, but not in spleen, brain, and testes. Genetic mapping confirmed the location of an egasyn cDNA fragment in cluster 1 of the esterase region on chromosome 8. Transfection of COS cells with the 2022-bp cDNA resulted in the expression of esterase activity, which comigrated on native gels with liver esterase-22. The features of the deduced amino acid sequence of the egasyn cDNA are compared with previously characterized carboxylesterases and with other lumenal ER proteins. |
| doi_str_mv | 10.1016/0888-7543(91)90020-F |
| format | Article |
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We utilized the polymerase chain reaction (PCR) in the eventual isolation of murine egasyn cDNAs. PCR primers were based upon: (1) partial amino acid sequences derived from egasyn peptides and (2) a conserved active site region shared by carboxylesterases. The amino acid sequence deduced from the PCR product matched that obtained from egasyn protein. This product was utilized as a probe to screen a cDNA library. Two cDNAs whose composite sequence encoded an open reading frame of 562 amino acids were isolated. A message size of 1700–2000 bp was revealed by RNA blot hybridization analysis. S1 nuclease protection analyses detected mRNA in liver, kidney, lung, and submandibular gland, but not in spleen, brain, and testes. Genetic mapping confirmed the location of an egasyn cDNA fragment in cluster 1 of the esterase region on chromosome 8. Transfection of COS cells with the 2022-bp cDNA resulted in the expression of esterase activity, which comigrated on native gels with liver esterase-22. The features of the deduced amino acid sequence of the egasyn cDNA are compared with previously characterized carboxylesterases and with other lumenal ER proteins.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1016/0888-7543(91)90020-F</identifier><identifier>PMID: 1783403</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; Blotting, Northern ; Carboxylic Ester Hydrolases - chemistry ; Carboxylic Ester Hydrolases - genetics ; Carboxylic Ester Hydrolases - isolation & purification ; Carboxylic Ester Hydrolases - metabolism ; Chromosomes, Human, Pair 8 ; Cloning, Molecular ; DNA - isolation & purification ; Endoplasmic Reticulum - enzymology ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; genes ; Genes. Genome ; Glucuronidase - metabolism ; Humans ; Liver - enzymology ; Male ; Membrane Glycoproteins - chemistry ; Membrane Glycoproteins - genetics ; Membrane Glycoproteins - isolation & purification ; Membrane Glycoproteins - metabolism ; Mice ; Mice, Inbred C57BL ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Organ Specificity - genetics ; Polymerase Chain Reaction ; Restriction Mapping ; Sequence Alignment</subject><ispartof>Genomics (San Diego, Calif.), 1991-12, Vol.11 (4), p.956-967</ispartof><rights>1991</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-3a039dbe1c83af06b295f748971be3ce61237698acb15e8a848c5e8e594845903</citedby><cites>FETCH-LOGICAL-c417t-3a039dbe1c83af06b295f748971be3ce61237698acb15e8a848c5e8e594845903</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0888-7543(91)90020-F$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5083721$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1783403$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ovnic, Mariana</creatorcontrib><creatorcontrib>Swank, Richard T.</creatorcontrib><creatorcontrib>Fletcher, Colin</creatorcontrib><creatorcontrib>Zhen, Lida</creatorcontrib><creatorcontrib>Novak, Edward K.</creatorcontrib><creatorcontrib>Baumann, Heinz</creatorcontrib><creatorcontrib>Heintz, Nathanial</creatorcontrib><creatorcontrib>Ganschow, Roger E.</creatorcontrib><title>Characterization and functional expression of a cDNA encoding egasyn (esterase-22): the endoplasmic reticulum-targeting protein of β-glucuronidase</title><title>Genomics (San Diego, Calif.)</title><addtitle>Genomics</addtitle><description>Egasyn (esterase-22), a member of the nonspecific carboxylesterase multigene family (E.C. 3.1.1.1.), is the endoplasmic reticulum (ER)-targeting protein of β-glucuronidase. We utilized the polymerase chain reaction (PCR) in the eventual isolation of murine egasyn cDNAs. PCR primers were based upon: (1) partial amino acid sequences derived from egasyn peptides and (2) a conserved active site region shared by carboxylesterases. The amino acid sequence deduced from the PCR product matched that obtained from egasyn protein. This product was utilized as a probe to screen a cDNA library. Two cDNAs whose composite sequence encoded an open reading frame of 562 amino acids were isolated. A message size of 1700–2000 bp was revealed by RNA blot hybridization analysis. S1 nuclease protection analyses detected mRNA in liver, kidney, lung, and submandibular gland, but not in spleen, brain, and testes. Genetic mapping confirmed the location of an egasyn cDNA fragment in cluster 1 of the esterase region on chromosome 8. Transfection of COS cells with the 2022-bp cDNA resulted in the expression of esterase activity, which comigrated on native gels with liver esterase-22. The features of the deduced amino acid sequence of the egasyn cDNA are compared with previously characterized carboxylesterases and with other lumenal ER proteins.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Carboxylic Ester Hydrolases - chemistry</subject><subject>Carboxylic Ester Hydrolases - genetics</subject><subject>Carboxylic Ester Hydrolases - isolation & purification</subject><subject>Carboxylic Ester Hydrolases - metabolism</subject><subject>Chromosomes, Human, Pair 8</subject><subject>Cloning, Molecular</subject><subject>DNA - isolation & purification</subject><subject>Endoplasmic Reticulum - enzymology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>genes</subject><subject>Genes. Genome</subject><subject>Glucuronidase - metabolism</subject><subject>Humans</subject><subject>Liver - enzymology</subject><subject>Male</subject><subject>Membrane Glycoproteins - chemistry</subject><subject>Membrane Glycoproteins - genetics</subject><subject>Membrane Glycoproteins - isolation & purification</subject><subject>Membrane Glycoproteins - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Organ Specificity - genetics</subject><subject>Polymerase Chain Reaction</subject><subject>Restriction Mapping</subject><subject>Sequence Alignment</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1uFDEQhS1EFIbADUDyAqFk4WC33W6bBVI0MCRSBBtYW9Xu6olR_wx2NyJcg5twEM6EOzMKO1iVSu97T2U_Qp4Jfi640K-4MYZVpZKnVpxZzgvONg_ISnBjmdFKPySre-QReZzSF865laY4JseiMlJxuSI_1zcQwU8Yww-YwjhQGBrazoNfFugoft9FTGlRxpYC9W8_XFAc_NiEYUtxC-l2oKeYcgIkZEVx9ppON5iRZtx1kPrgacQp-LmbezZB3OYlO3dxnDDchf7-xbbd7Oc4DqHJIU_IUQtdwqeHeUI-b959Wl-y64_vr9YX18wrUU1MApe2qVF4I6Hlui5s2VbK2ErUKD1qUchKWwO-FiUaMMr4PLG0yqjScnlCXu5z8y1f5_wE14fksetgwHFOriq0kkX1f1Boro2QJoNqD_o4phSxdbsYeoi3TnC3lOaWRtzSiLPC3ZXmNtn2_JA_1z02f037lrL-4qBD8tC1EQYf0j1WciOrQmTszR7D_GnfAkaXfMhVYRMi-sk1Y_j3HX8ASf-1ag</recordid><startdate>19911201</startdate><enddate>19911201</enddate><creator>Ovnic, Mariana</creator><creator>Swank, Richard T.</creator><creator>Fletcher, Colin</creator><creator>Zhen, Lida</creator><creator>Novak, Edward K.</creator><creator>Baumann, Heinz</creator><creator>Heintz, Nathanial</creator><creator>Ganschow, Roger E.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19911201</creationdate><title>Characterization and functional expression of a cDNA encoding egasyn (esterase-22): the endoplasmic reticulum-targeting protein of β-glucuronidase</title><author>Ovnic, Mariana ; Swank, Richard T. ; Fletcher, Colin ; Zhen, Lida ; Novak, Edward K. ; Baumann, Heinz ; Heintz, Nathanial ; Ganschow, Roger E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-3a039dbe1c83af06b295f748971be3ce61237698acb15e8a848c5e8e594845903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Carboxylic Ester Hydrolases - chemistry</topic><topic>Carboxylic Ester Hydrolases - genetics</topic><topic>Carboxylic Ester Hydrolases - isolation & purification</topic><topic>Carboxylic Ester Hydrolases - metabolism</topic><topic>Chromosomes, Human, Pair 8</topic><topic>Cloning, Molecular</topic><topic>DNA - isolation & purification</topic><topic>Endoplasmic Reticulum - enzymology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>genes</topic><topic>Genes. Genome</topic><topic>Glucuronidase - metabolism</topic><topic>Humans</topic><topic>Liver - enzymology</topic><topic>Male</topic><topic>Membrane Glycoproteins - chemistry</topic><topic>Membrane Glycoproteins - genetics</topic><topic>Membrane Glycoproteins - isolation & purification</topic><topic>Membrane Glycoproteins - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Organ Specificity - genetics</topic><topic>Polymerase Chain Reaction</topic><topic>Restriction Mapping</topic><topic>Sequence Alignment</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ovnic, Mariana</creatorcontrib><creatorcontrib>Swank, Richard T.</creatorcontrib><creatorcontrib>Fletcher, Colin</creatorcontrib><creatorcontrib>Zhen, Lida</creatorcontrib><creatorcontrib>Novak, Edward K.</creatorcontrib><creatorcontrib>Baumann, Heinz</creatorcontrib><creatorcontrib>Heintz, Nathanial</creatorcontrib><creatorcontrib>Ganschow, Roger E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genomics (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ovnic, Mariana</au><au>Swank, Richard T.</au><au>Fletcher, Colin</au><au>Zhen, Lida</au><au>Novak, Edward K.</au><au>Baumann, Heinz</au><au>Heintz, Nathanial</au><au>Ganschow, Roger E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization and functional expression of a cDNA encoding egasyn (esterase-22): the endoplasmic reticulum-targeting protein of β-glucuronidase</atitle><jtitle>Genomics (San Diego, Calif.)</jtitle><addtitle>Genomics</addtitle><date>1991-12-01</date><risdate>1991</risdate><volume>11</volume><issue>4</issue><spage>956</spage><epage>967</epage><pages>956-967</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>Egasyn (esterase-22), a member of the nonspecific carboxylesterase multigene family (E.C. 3.1.1.1.), is the endoplasmic reticulum (ER)-targeting protein of β-glucuronidase. We utilized the polymerase chain reaction (PCR) in the eventual isolation of murine egasyn cDNAs. PCR primers were based upon: (1) partial amino acid sequences derived from egasyn peptides and (2) a conserved active site region shared by carboxylesterases. The amino acid sequence deduced from the PCR product matched that obtained from egasyn protein. This product was utilized as a probe to screen a cDNA library. Two cDNAs whose composite sequence encoded an open reading frame of 562 amino acids were isolated. A message size of 1700–2000 bp was revealed by RNA blot hybridization analysis. S1 nuclease protection analyses detected mRNA in liver, kidney, lung, and submandibular gland, but not in spleen, brain, and testes. Genetic mapping confirmed the location of an egasyn cDNA fragment in cluster 1 of the esterase region on chromosome 8. Transfection of COS cells with the 2022-bp cDNA resulted in the expression of esterase activity, which comigrated on native gels with liver esterase-22. The features of the deduced amino acid sequence of the egasyn cDNA are compared with previously characterized carboxylesterases and with other lumenal ER proteins.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1783403</pmid><doi>10.1016/0888-7543(91)90020-F</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0888-7543 |
| ispartof | Genomics (San Diego, Calif.), 1991-12, Vol.11 (4), p.956-967 |
| issn | 0888-7543 1089-8646 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Amino Acid Sequence Animals Base Sequence Biological and medical sciences Blotting, Northern Carboxylic Ester Hydrolases - chemistry Carboxylic Ester Hydrolases - genetics Carboxylic Ester Hydrolases - isolation & purification Carboxylic Ester Hydrolases - metabolism Chromosomes, Human, Pair 8 Cloning, Molecular DNA - isolation & purification Endoplasmic Reticulum - enzymology Female Fundamental and applied biological sciences. Psychology Gene Expression genes Genes. Genome Glucuronidase - metabolism Humans Liver - enzymology Male Membrane Glycoproteins - chemistry Membrane Glycoproteins - genetics Membrane Glycoproteins - isolation & purification Membrane Glycoproteins - metabolism Mice Mice, Inbred C57BL Molecular and cellular biology Molecular genetics Molecular Sequence Data Organ Specificity - genetics Polymerase Chain Reaction Restriction Mapping Sequence Alignment |
| title | Characterization and functional expression of a cDNA encoding egasyn (esterase-22): the endoplasmic reticulum-targeting protein of β-glucuronidase |
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