Cloning and Expression of cDNAs from Enterically-Transmitted Non-A, Non-B Hepatitis Virus
The fragment gene of enterically-transmitted non-A, non-B hepatitis virus (ET-NANBHV) was cloned as a cDNA and inserted into an expression vector pUEX2. The recombinant protein was expressed in Escherichia coli HB101 as a fusion protein with β-galactosidase (β-Gal). The fusion protein reacted with t...
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| Veröffentlicht in: | MICROBIOLOGY and IMMUNOLOGY 1991, Vol.35(7), pp.535-543 |
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| container_title | MICROBIOLOGY and IMMUNOLOGY |
| container_volume | 35 |
| creator | Ichikawa, Munetaka Araki, Masatake Rikihisa, Tetsuji Uchida, Toshikazu Shikata, Toshio Mizuno, Kyosuke |
| description | The fragment gene of enterically-transmitted non-A, non-B hepatitis virus (ET-NANBHV) was cloned as a cDNA and inserted into an expression vector pUEX2. The recombinant protein was expressed in Escherichia coli HB101 as a fusion protein with β-galactosidase (β-Gal). The fusion protein reacted with the sera of infected cynomolgus monkeys and of patients from Myanmar. This reaction was highly related with ET-NANBHV infection, and obviously demonstrates in that the recombinant protein can be used for the detection of ET-NANBHV infection. |
| doi_str_mv | 10.1111/j.1348-0421.1991.tb01584.x |
| format | Article |
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The recombinant protein was expressed in Escherichia coli HB101 as a fusion protein with β-galactosidase (β-Gal). The fusion protein reacted with the sera of infected cynomolgus monkeys and of patients from Myanmar. This reaction was highly related with ET-NANBHV infection, and obviously demonstrates in that the recombinant protein can be used for the detection of ET-NANBHV infection.</description><identifier>ISSN: 0385-5600</identifier><identifier>EISSN: 1348-0421</identifier><identifier>DOI: 10.1111/j.1348-0421.1991.tb01584.x</identifier><identifier>PMID: 1784255</identifier><identifier>CODEN: MIIMDV</identifier><language>eng</language><publisher>Tokyo: Blackwell Publishing Ltd</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; Blotting, Northern ; Blotting, Southern ; Blotting, Western ; Cloning, Molecular ; Cynomolgus ; DNA - genetics ; Escherichia coli - genetics ; Expression vectors ; Fundamental and applied biological sciences. Psychology ; Genes, Viral ; Genetics ; Hepatitis Antibodies - analysis ; Hepatitis E - immunology ; Hepatitis E virus - genetics ; Hepatitis E virus - immunology ; Humans ; Macaca fascicularis ; Microbiology ; Microscopy, Electron ; Molecular Sequence Data ; Recombinant Fusion Proteins - biosynthesis ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - immunology ; Viral Proteins - genetics ; Viral Proteins - immunology ; Virology</subject><ispartof>MICROBIOLOGY and IMMUNOLOGY, 1991, Vol.35(7), pp.535-543</ispartof><rights>Center for Academic Publications Japan</rights><rights>owned by Center for Academic Publications Japan (Publisher)</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6894-952820af49b307706788685d25c86bb916baca809ba3d7447d23f516756240da3</citedby><cites>FETCH-LOGICAL-c6894-952820af49b307706788685d25c86bb916baca809ba3d7447d23f516756240da3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5112105$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1784255$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ichikawa, Munetaka</creatorcontrib><creatorcontrib>Araki, Masatake</creatorcontrib><creatorcontrib>Rikihisa, Tetsuji</creatorcontrib><creatorcontrib>Uchida, Toshikazu</creatorcontrib><creatorcontrib>Shikata, Toshio</creatorcontrib><creatorcontrib>Mizuno, Kyosuke</creatorcontrib><title>Cloning and Expression of cDNAs from Enterically-Transmitted Non-A, Non-B Hepatitis Virus</title><title>MICROBIOLOGY and IMMUNOLOGY</title><addtitle>Microbiology and Immunology</addtitle><description>The fragment gene of enterically-transmitted non-A, non-B hepatitis virus (ET-NANBHV) was cloned as a cDNA and inserted into an expression vector pUEX2. The recombinant protein was expressed in Escherichia coli HB101 as a fusion protein with β-galactosidase (β-Gal). The fusion protein reacted with the sera of infected cynomolgus monkeys and of patients from Myanmar. This reaction was highly related with ET-NANBHV infection, and obviously demonstrates in that the recombinant protein can be used for the detection of ET-NANBHV infection.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Blotting, Southern</subject><subject>Blotting, Western</subject><subject>Cloning, Molecular</subject><subject>Cynomolgus</subject><subject>DNA - genetics</subject><subject>Escherichia coli - genetics</subject><subject>Expression vectors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Viral</subject><subject>Genetics</subject><subject>Hepatitis Antibodies - analysis</subject><subject>Hepatitis E - immunology</subject><subject>Hepatitis E virus - genetics</subject><subject>Hepatitis E virus - immunology</subject><subject>Humans</subject><subject>Macaca fascicularis</subject><subject>Microbiology</subject><subject>Microscopy, Electron</subject><subject>Molecular Sequence Data</subject><subject>Recombinant Fusion Proteins - biosynthesis</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - immunology</subject><subject>Viral Proteins - genetics</subject><subject>Viral Proteins - immunology</subject><subject>Virology</subject><issn>0385-5600</issn><issn>1348-0421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVktuO0zAQhi0EWsrCIyBFCCEuSPD5wBWl2z1I3SKkBcSV5STO4pJDsVPRvj3OJhSuQPhixtJ883s0vwF4hmCG4nm9yRChMoUUowwphbI-h4hJmu3vgdmxdB_MIJEsZRzCh-BRCBsIscCSnoATJCTFjM3Al0Xdta69TUxbJsv91tsQXNcmXZUUZ-t5SCrfNcmy7a13hanrQ3rjTRsa1_e2TNZdm85f3aV3yaXdmt71LiSfnN-Fx-BBZepgn0z5FHw8X94sLtPV-4urxXyVFlwqmiqGJYamoionUAjIhZRcshKzQvI8V4jnpjASqtyQUlAqSkwqhrhgHFNYGnIKXoy6W99939nQ68aFwta1aW23C1pgThFm4p8g4gwThlAEX_4dpApzRDClEX0zooXvQvC20lvvGuMPGkE9eKU3ejBED4bowSs9eaX3sfnp9M4ub2z5u3U0J9afT3UT4u6ruPjChSMWR8UIDtjbEfvhanv4jwH09dX13TVKLEeJTejNrT1qGN-7ora6ib_DISWEJkxPgcX4q158NV7bNuqko44Lvd3_IfNNc0EE05_XF1qtVlzCsw_6nPwE3CrVmA</recordid><startdate>19910101</startdate><enddate>19910101</enddate><creator>Ichikawa, Munetaka</creator><creator>Araki, Masatake</creator><creator>Rikihisa, Tetsuji</creator><creator>Uchida, Toshikazu</creator><creator>Shikata, Toshio</creator><creator>Mizuno, Kyosuke</creator><general>Blackwell Publishing Ltd</general><general>Center For Academic Publications Japan</general><general>Center for Academic Publications Japan</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19910101</creationdate><title>Cloning and Expression of cDNAs from Enterically-Transmitted Non-A, Non-B Hepatitis Virus</title><author>Ichikawa, Munetaka ; Araki, Masatake ; Rikihisa, Tetsuji ; Uchida, Toshikazu ; Shikata, Toshio ; Mizuno, Kyosuke</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6894-952820af49b307706788685d25c86bb916baca809ba3d7447d23f516756240da3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Blotting, Southern</topic><topic>Blotting, Western</topic><topic>Cloning, Molecular</topic><topic>Cynomolgus</topic><topic>DNA - genetics</topic><topic>Escherichia coli - genetics</topic><topic>Expression vectors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Viral</topic><topic>Genetics</topic><topic>Hepatitis Antibodies - analysis</topic><topic>Hepatitis E - immunology</topic><topic>Hepatitis E virus - genetics</topic><topic>Hepatitis E virus - immunology</topic><topic>Humans</topic><topic>Macaca fascicularis</topic><topic>Microbiology</topic><topic>Microscopy, Electron</topic><topic>Molecular Sequence Data</topic><topic>Recombinant Fusion Proteins - biosynthesis</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - immunology</topic><topic>Viral Proteins - genetics</topic><topic>Viral Proteins - immunology</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ichikawa, Munetaka</creatorcontrib><creatorcontrib>Araki, Masatake</creatorcontrib><creatorcontrib>Rikihisa, Tetsuji</creatorcontrib><creatorcontrib>Uchida, Toshikazu</creatorcontrib><creatorcontrib>Shikata, Toshio</creatorcontrib><creatorcontrib>Mizuno, Kyosuke</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>MICROBIOLOGY and IMMUNOLOGY</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ichikawa, Munetaka</au><au>Araki, Masatake</au><au>Rikihisa, Tetsuji</au><au>Uchida, Toshikazu</au><au>Shikata, Toshio</au><au>Mizuno, Kyosuke</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and Expression of cDNAs from Enterically-Transmitted Non-A, Non-B Hepatitis Virus</atitle><jtitle>MICROBIOLOGY and IMMUNOLOGY</jtitle><addtitle>Microbiology and Immunology</addtitle><date>1991-01-01</date><risdate>1991</risdate><volume>35</volume><issue>7</issue><spage>535</spage><epage>543</epage><pages>535-543</pages><issn>0385-5600</issn><eissn>1348-0421</eissn><coden>MIIMDV</coden><abstract>The fragment gene of enterically-transmitted non-A, non-B hepatitis virus (ET-NANBHV) was cloned as a cDNA and inserted into an expression vector pUEX2. The recombinant protein was expressed in Escherichia coli HB101 as a fusion protein with β-galactosidase (β-Gal). The fusion protein reacted with the sera of infected cynomolgus monkeys and of patients from Myanmar. This reaction was highly related with ET-NANBHV infection, and obviously demonstrates in that the recombinant protein can be used for the detection of ET-NANBHV infection.</abstract><cop>Tokyo</cop><pub>Blackwell Publishing Ltd</pub><pmid>1784255</pmid><doi>10.1111/j.1348-0421.1991.tb01584.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | J-STAGE Free; MEDLINE; Freely Accessible Japanese Titles; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Animals Base Sequence Biological and medical sciences Blotting, Northern Blotting, Southern Blotting, Western Cloning, Molecular Cynomolgus DNA - genetics Escherichia coli - genetics Expression vectors Fundamental and applied biological sciences. Psychology Genes, Viral Genetics Hepatitis Antibodies - analysis Hepatitis E - immunology Hepatitis E virus - genetics Hepatitis E virus - immunology Humans Macaca fascicularis Microbiology Microscopy, Electron Molecular Sequence Data Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - immunology Viral Proteins - genetics Viral Proteins - immunology Virology |
| title | Cloning and Expression of cDNAs from Enterically-Transmitted Non-A, Non-B Hepatitis Virus |
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