NAN fusions: a synthetic sialidase reporter gene as a sensitive and versatile partner for GUS

Summary GUS continues to be the reporter of choice for many gene fusion applications, due to the unparalleled sensitivity of the encoded enzyme and the ease with which it can be quantified in cell‐free extracts and visualized histochemically in cells and tissues. A compatible and functionally equiva...

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Veröffentlicht in:	The Plant journal : for cell and molecular biology 2002-11, Vol.32 (3), p.391-400
Hauptverfasser:	Kirby, J., Kavanagh, T. A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Base Sequence Biological and medical sciences Diverse techniques Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Plant Genes, Reporter - genetics Glucuronidase - genetics Glucuronidase - metabolism GUS Histocytochemistry Hydrogen-Ion Concentration Molecular and cellular biology Molecular Sequence Data NAN Neuraminidase - genetics Neuraminidase - metabolism Nicotiana - genetics Nicotiana - metabolism Plants, Genetically Modified Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism reporter genes sialidase synthetic gene transgenic plants
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