An h.p.l.c. method for determining chain-length distribution in some glycogens
Human, oyster, Streptococcus mitis, and phyto-glycogen samples were debranched using Pseudomonas amylodermosa isoamylase (EC 3.2.1.68). The distribution of chain lengths was studied by high-performance liquid chromatography on reversed-phase columns, with water as eluent. Quantitative data was obtai...
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| Veröffentlicht in: | Carbohydrate research 1991-08, Vol.215 (1), p.59-65 |
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| container_title | Carbohydrate research |
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| creator | Cheetham, Norman W.H. Hansawek, Nanthavan Saecou, Pranee |
| description | Human, oyster,
Streptococcus mitis, and phyto-glycogen samples were debranched using
Pseudomonas amylodermosa isoamylase (EC 3.2.1.68). The distribution of chain lengths was studied by high-performance liquid chromatography on reversed-phase columns, with water as eluent. Quantitative data was obtained over the degree of polymerisation range three to eighteen (d.p. 3–18), and oligosaccharides up to d.p. 26 were detected. No single column was found suitable for the resolution of the complete range of oligosaccharides, two columns being necessary for the quantitative analysis. The resulting “fingerprints” of chain lengths are characteristic of the glycogen source and should be useful for both comparison purposes among glycogens and for monitoring procedures of glycogen isolation. |
| doi_str_mv | 10.1016/0008-6215(91)84007-2 |
| format | Article |
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Streptococcus mitis, and phyto-glycogen samples were debranched using
Pseudomonas amylodermosa isoamylase (EC 3.2.1.68). The distribution of chain lengths was studied by high-performance liquid chromatography on reversed-phase columns, with water as eluent. Quantitative data was obtained over the degree of polymerisation range three to eighteen (d.p. 3–18), and oligosaccharides up to d.p. 26 were detected. No single column was found suitable for the resolution of the complete range of oligosaccharides, two columns being necessary for the quantitative analysis. The resulting “fingerprints” of chain lengths are characteristic of the glycogen source and should be useful for both comparison purposes among glycogens and for monitoring procedures of glycogen isolation.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycogen - chemistry</subject><subject>Glycogen - isolation & purification</subject><subject>Humans</subject><subject>Isoamylase</subject><subject>Molecular Structure</subject><subject>Molecular Weight</subject><subject>Oligosaccharides - chemistry</subject><subject>Oligosaccharides - isolation & purification</subject><issn>0008-6215</issn><issn>1873-426X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM9LHDEUgENR7Gr7H7SQg4geZppkMknmIoi0VRC9WOgtZJKX3ZSZZJvMCvvfd9Zd7M3Lezze937wIfSFkpoSKr4RQlQlGG0vO3qlOCGyYh_QgirZVJyJ30do8YZ8RKel_JlLIqQ4QSdUKtEqskCPNxGv6nU91LbGI0yr5LBPGTuYII8hhrjEdmVCrAaIy2mFXShTDv1mCiniEHFJI-DlsLVpCbF8QsfeDAU-H_IZ-vXj-_PtXfXw9PP-9uahsrxlUyV8Y6RSwPwchegNIa7xoun7zhElGWOOctfxVnlHTS_aljPgnrGOSuuJas7QxX7vOqe_GyiTHkOxMAwmQtoULZloukayGeR70OZUSgav1zmMJm81JXqnUe8c6Z0j3VH9qlHvxr4e9m_6Edz_ob23uX9-6JtizeCziTaUN6zlksnX69d7DGYXLwGyLjZAtOBCBjtpl8L7f_wDdSqM1w</recordid><startdate>19910812</startdate><enddate>19910812</enddate><creator>Cheetham, Norman W.H.</creator><creator>Hansawek, Nanthavan</creator><creator>Saecou, Pranee</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19910812</creationdate><title>An h.p.l.c. method for determining chain-length distribution in some glycogens</title><author>Cheetham, Norman W.H. ; Hansawek, Nanthavan ; Saecou, Pranee</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-6f3a788e2f78866ba00d3f63bb9d087222d14d9458fd1ab65542e4f22917cf083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycogen - chemistry</topic><topic>Glycogen - isolation & purification</topic><topic>Humans</topic><topic>Isoamylase</topic><topic>Molecular Structure</topic><topic>Molecular Weight</topic><topic>Oligosaccharides - chemistry</topic><topic>Oligosaccharides - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cheetham, Norman W.H.</creatorcontrib><creatorcontrib>Hansawek, Nanthavan</creatorcontrib><creatorcontrib>Saecou, Pranee</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Carbohydrate research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cheetham, Norman W.H.</au><au>Hansawek, Nanthavan</au><au>Saecou, Pranee</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An h.p.l.c. method for determining chain-length distribution in some glycogens</atitle><jtitle>Carbohydrate research</jtitle><addtitle>Carbohydr Res</addtitle><date>1991-08-12</date><risdate>1991</risdate><volume>215</volume><issue>1</issue><spage>59</spage><epage>65</epage><pages>59-65</pages><issn>0008-6215</issn><eissn>1873-426X</eissn><coden>CRBRAT</coden><abstract>Human, oyster,
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Pseudomonas amylodermosa isoamylase (EC 3.2.1.68). The distribution of chain lengths was studied by high-performance liquid chromatography on reversed-phase columns, with water as eluent. Quantitative data was obtained over the degree of polymerisation range three to eighteen (d.p. 3–18), and oligosaccharides up to d.p. 26 were detected. No single column was found suitable for the resolution of the complete range of oligosaccharides, two columns being necessary for the quantitative analysis. The resulting “fingerprints” of chain lengths are characteristic of the glycogen source and should be useful for both comparison purposes among glycogens and for monitoring procedures of glycogen isolation.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>1786580</pmid><doi>10.1016/0008-6215(91)84007-2</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Carbohydrate research, 1991-08, Vol.215 (1), p.59-65 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Chromatography, High Pressure Liquid - methods Fundamental and applied biological sciences. Psychology Glycogen - chemistry Glycogen - isolation & purification Humans Isoamylase Molecular Structure Molecular Weight Oligosaccharides - chemistry Oligosaccharides - isolation & purification |
| title | An h.p.l.c. method for determining chain-length distribution in some glycogens |
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