Identification of the novel splicing variants for the hPXR in human livers
The human pregnane X receptor (hPXR) plays a key role in the regulation of both drug metabolism and efflux by inducing the expression of CYP3A4 and MDR1 gene. Using reverse transcription-polymerase chain reaction (RT-PCR) analysis, we identified seven novel splicing variants of hPXR in tissue from a...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2002-11, Vol.298 (3), p.433-438 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Fukuen, Shuichi Fukuda, Tsuyoshi Matsuda, Hideyasu Sumida, Akihiko Yamamoto, Isamu Inaba, Tadanobu Azuma, Junichi |
| description | The human pregnane X receptor (hPXR) plays a key role in the regulation of both drug metabolism and efflux by inducing the expression of
CYP3A4 and
MDR1 gene. Using reverse transcription-polymerase chain reaction (RT-PCR) analysis, we identified seven novel splicing variants of hPXR in tissue from a single human liver. The expression of hPXR-related transcripts in the liver samples of 15 Caucasian individuals was subsequently determined by RT-PCR assays. The pattern of expression levels of these transcripts varied among liver samples. These results suggest that the hPXR is expressed as several different transcripts in liver tissues, apparently due to alternative as well as defective gene splicing. Furthermore, because this study provides the possibility of interindividual differences in hPXR transcript profiles, these alternative splicings for hPXR may largely contribute to the interindividual variability in CYP3A4 and
P-glycoprotein induction. |
| doi_str_mv | 10.1016/S0006-291X(02)02469-5 |
| format | Article |
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CYP3A4 and
MDR1 gene. Using reverse transcription-polymerase chain reaction (RT-PCR) analysis, we identified seven novel splicing variants of hPXR in tissue from a single human liver. The expression of hPXR-related transcripts in the liver samples of 15 Caucasian individuals was subsequently determined by RT-PCR assays. The pattern of expression levels of these transcripts varied among liver samples. These results suggest that the hPXR is expressed as several different transcripts in liver tissues, apparently due to alternative as well as defective gene splicing. Furthermore, because this study provides the possibility of interindividual differences in hPXR transcript profiles, these alternative splicings for hPXR may largely contribute to the interindividual variability in CYP3A4 and
P-glycoprotein induction.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/S0006-291X(02)02469-5</identifier><identifier>PMID: 12413960</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Alternative Splicing ; Base Sequence ; CYP3A4 ; DNA Primers ; Drug metabolism ; hPXR ; Human liver ; Humans ; Liver - metabolism ; P-Glycoprotein ; Pregnane X Receptor ; Receptors, Cytoplasmic and Nuclear - genetics ; Receptors, Steroid - genetics ; Reverse Transcriptase Polymerase Chain Reaction ; RT-PCR ; Splicing variant</subject><ispartof>Biochemical and biophysical research communications, 2002-11, Vol.298 (3), p.433-438</ispartof><rights>2002 Elsevier Science (USA)</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c458t-e6b695d38899d2524fc6122729e107f24ef12666addbd00c50d5350430f8a3d83</citedby><cites>FETCH-LOGICAL-c458t-e6b695d38899d2524fc6122729e107f24ef12666addbd00c50d5350430f8a3d83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0006-291X(02)02469-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12413960$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fukuen, Shuichi</creatorcontrib><creatorcontrib>Fukuda, Tsuyoshi</creatorcontrib><creatorcontrib>Matsuda, Hideyasu</creatorcontrib><creatorcontrib>Sumida, Akihiko</creatorcontrib><creatorcontrib>Yamamoto, Isamu</creatorcontrib><creatorcontrib>Inaba, Tadanobu</creatorcontrib><creatorcontrib>Azuma, Junichi</creatorcontrib><title>Identification of the novel splicing variants for the hPXR in human livers</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The human pregnane X receptor (hPXR) plays a key role in the regulation of both drug metabolism and efflux by inducing the expression of
CYP3A4 and
MDR1 gene. Using reverse transcription-polymerase chain reaction (RT-PCR) analysis, we identified seven novel splicing variants of hPXR in tissue from a single human liver. The expression of hPXR-related transcripts in the liver samples of 15 Caucasian individuals was subsequently determined by RT-PCR assays. The pattern of expression levels of these transcripts varied among liver samples. These results suggest that the hPXR is expressed as several different transcripts in liver tissues, apparently due to alternative as well as defective gene splicing. Furthermore, because this study provides the possibility of interindividual differences in hPXR transcript profiles, these alternative splicings for hPXR may largely contribute to the interindividual variability in CYP3A4 and
P-glycoprotein induction.</description><subject>Alternative Splicing</subject><subject>Base Sequence</subject><subject>CYP3A4</subject><subject>DNA Primers</subject><subject>Drug metabolism</subject><subject>hPXR</subject><subject>Human liver</subject><subject>Humans</subject><subject>Liver - metabolism</subject><subject>P-Glycoprotein</subject><subject>Pregnane X Receptor</subject><subject>Receptors, Cytoplasmic and Nuclear - genetics</subject><subject>Receptors, Steroid - genetics</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RT-PCR</subject><subject>Splicing variant</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0EtLAzEQwPEgitbHR1ByEj2sTrKbNHsSEZ8Iig_wFtJkYiPbbE22Bb-9a1v06CmH_GYG_oTsMzhhwOTpMwDIgtfs7Qj4MfBK1oVYIwMGNRScQbVOBr9ki2zn_AHAWM82yRbjFStrCQNyd-swdsEHa7rQRtp62o2RxnaODc3TJtgQ3-ncpGBil6lv0-J__Pj2REOk49nERNqEOaa8Sza8aTLurd4d8np1-XJxU9w_XN9enN8XthKqK1COZC1cqVRdOy545a1knA95jQyGnlfoGZdSGudGDsAKcKIUUJXglSmdKnfI4XLvNLWfM8ydnoRssWlMxHaW9ZDLUggl_4WsN0op6KFYQpvanBN6PU1hYtKXZqB_autFbf2TUgPXi9pa9HMHqwOz0QTd39Qqbw_OlgD7HvOASWcbMFp0IaHttGvDPye-AYjPjc8</recordid><startdate>20021101</startdate><enddate>20021101</enddate><creator>Fukuen, Shuichi</creator><creator>Fukuda, Tsuyoshi</creator><creator>Matsuda, Hideyasu</creator><creator>Sumida, Akihiko</creator><creator>Yamamoto, Isamu</creator><creator>Inaba, Tadanobu</creator><creator>Azuma, Junichi</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20021101</creationdate><title>Identification of the novel splicing variants for the hPXR in human livers</title><author>Fukuen, Shuichi ; Fukuda, Tsuyoshi ; Matsuda, Hideyasu ; Sumida, Akihiko ; Yamamoto, Isamu ; Inaba, Tadanobu ; Azuma, Junichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-e6b695d38899d2524fc6122729e107f24ef12666addbd00c50d5350430f8a3d83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Alternative Splicing</topic><topic>Base Sequence</topic><topic>CYP3A4</topic><topic>DNA Primers</topic><topic>Drug metabolism</topic><topic>hPXR</topic><topic>Human liver</topic><topic>Humans</topic><topic>Liver - metabolism</topic><topic>P-Glycoprotein</topic><topic>Pregnane X Receptor</topic><topic>Receptors, Cytoplasmic and Nuclear - genetics</topic><topic>Receptors, Steroid - genetics</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RT-PCR</topic><topic>Splicing variant</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fukuen, Shuichi</creatorcontrib><creatorcontrib>Fukuda, Tsuyoshi</creatorcontrib><creatorcontrib>Matsuda, Hideyasu</creatorcontrib><creatorcontrib>Sumida, Akihiko</creatorcontrib><creatorcontrib>Yamamoto, Isamu</creatorcontrib><creatorcontrib>Inaba, Tadanobu</creatorcontrib><creatorcontrib>Azuma, Junichi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fukuen, Shuichi</au><au>Fukuda, Tsuyoshi</au><au>Matsuda, Hideyasu</au><au>Sumida, Akihiko</au><au>Yamamoto, Isamu</au><au>Inaba, Tadanobu</au><au>Azuma, Junichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of the novel splicing variants for the hPXR in human livers</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2002-11-01</date><risdate>2002</risdate><volume>298</volume><issue>3</issue><spage>433</spage><epage>438</epage><pages>433-438</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The human pregnane X receptor (hPXR) plays a key role in the regulation of both drug metabolism and efflux by inducing the expression of
CYP3A4 and
MDR1 gene. Using reverse transcription-polymerase chain reaction (RT-PCR) analysis, we identified seven novel splicing variants of hPXR in tissue from a single human liver. The expression of hPXR-related transcripts in the liver samples of 15 Caucasian individuals was subsequently determined by RT-PCR assays. The pattern of expression levels of these transcripts varied among liver samples. These results suggest that the hPXR is expressed as several different transcripts in liver tissues, apparently due to alternative as well as defective gene splicing. Furthermore, because this study provides the possibility of interindividual differences in hPXR transcript profiles, these alternative splicings for hPXR may largely contribute to the interindividual variability in CYP3A4 and
P-glycoprotein induction.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>12413960</pmid><doi>10.1016/S0006-291X(02)02469-5</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 2002-11, Vol.298 (3), p.433-438 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Alternative Splicing Base Sequence CYP3A4 DNA Primers Drug metabolism hPXR Human liver Humans Liver - metabolism P-Glycoprotein Pregnane X Receptor Receptors, Cytoplasmic and Nuclear - genetics Receptors, Steroid - genetics Reverse Transcriptase Polymerase Chain Reaction RT-PCR Splicing variant |
| title | Identification of the novel splicing variants for the hPXR in human livers |
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