In vitro refolding process of urea-denatured microbial transglutaminase without pro-peptide sequence

Efficient refolding process of denatured mature microbial transglutaminase (MTG) without pro-peptide sequence was studied in the model system using urea-denatured pure MTG. Recombinant MTG, produced and purified to homogeneity according to the protocol previously reported, was denatured with 8 M ure...

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Veröffentlicht in:	Protein expression and purification 2002-11, Vol.26 (2), p.329-335
Hauptverfasser:	Yokoyama, Kei-ichi, Kunio, Ono, Ohtsuka, Tomoko, Nakamura, Nami, Seguro, Katsuya, Ejima, Daisuke
Format:	Artikel
Sprache:	eng
Schlagworte:	Escherichia coli Hydrogen-Ion Concentration Protein Denaturation Protein Folding Refolding Temperature Texture Transglutaminase Transglutaminases - chemistry Transglutaminases - metabolism Urea - chemistry
Online-Zugang:	Volltext
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