Conformational correlatives of DNA band compression and bidirectional migration during field inversion gel electrophoresis, detected by quantitative video epifluorescence microscopy

Individual DNA molecules in the Mb size range were monitored by epifluorescence video microscopy during field inversion gel electrophoresis (FIGE). DNA migrating in an agarose gel gives rise to characteristic V‐conformational elements and when doing so exhibits a reduced mobility. When the V‐conform...

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Veröffentlicht in:	Biopolymers 1991-10, Vol.31 (11), p.1297-1307
Hauptverfasser:	Rampino, Nicholas J., Chrambach, Andreas
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences DNA - chemistry Dna, deoxyribonucleoproteins Electrophoresis, Gel, Pulsed-Field - methods Fundamental and applied biological sciences. Psychology Microscopy, Fluorescence Nucleic Acid Conformation Nucleic acids Photomicrography Videotape Recording
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