Sensitized immunochemical method for the specific detection of human haemoglobin in dried biological samples
The authors describe a modified version of counterimmunoelectrophoresis of easy performance and high sensitivity. With this method human haemoglobin can easily and specifically be determined either in fresh or dried biological samples. Glutaraldehyde pretreatment of the samples results in the develo...
Gespeichert in:
| Veröffentlicht in: | Journal of clinical laboratory analysis 1991, Vol.5 (6), p.373-377 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 377 |
|---|---|
| container_issue | 6 |
| container_start_page | 373 |
| container_title | Journal of clinical laboratory analysis |
| container_volume | 5 |
| creator | Ottó, Szabolcs Németh, Mária Sz Kocsis, György Tulok, István Kelemen, Katalin |
| description | The authors describe a modified version of counterimmunoelectrophoresis of easy performance and high sensitivity. With this method human haemoglobin can easily and specifically be determined either in fresh or dried biological samples. Glutaraldehyde pretreatment of the samples results in the development of complexes with highly favourable electrophoretic mobility and precipitating capacity. By this chemical modification and use of a double set of samples with varying antigen‐antibody, proportion, the sensitivity of haemoglobin detection in erythrocyte‐containing haemolysate and native blood was 300 ng/ml. The examination is easily performed, and without considerable outlay, even with conventional laboratory facilities. It is equally suitable for the detection of occult colorectal bleeding and for species‐specific study of blood stains of unknown origin.
The authors succeeded in identifying human haemoglobin even in a 42‐month dried blood stain. |
| doi_str_mv | 10.1002/jcla.1860050602 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72621949</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>16397323</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4132-38a4363ae7934a2ad719e4a695238202c6744b2c8e60ef6e8d7e2317585ca51a3</originalsourceid><addsrcrecordid>eNqFkUFv1DAQRi1EVZbCmROST9zSju3EdsSpWkFLtaJFgMrN8jqTxsWOlzgRlF_flFRUPVUaaQ7zvneYj5A3DA4ZAD-6dsEeMi0BKpDAn5EVg1oXXPPqOVmB1qrQwMQL8jLnawDQNZP7ZJ8pVUpgKxK-Yp_96P9iQ32MU59ch9E7G2jEsUsNbdNAxw5p3qHzrXe0wRHd6FNPU0u7KdqedhZjugpp63s6TzP4Wbf1KaSrf6ps4y5gfkX2Whsyvr7fB-T7xw_f1qfF5vzk0_p4U7iSCV4IbUshhUVVi9Jy2yhWY2llXXGhOXAnVVluudMoAVuJulHIBVOVrpytmBUH5N3i3Q3p14R5NNFnhyHYHtOUjeKSs7qsnwSZFLUSXMzg0QK6IeU8YGt2g492uDEMzF0R5q4I81DEnHh7r562EZsHfvn8fH-_3H_7gDdP6czZenP8yF4saZ9H_PM_bYefRiqhKnP5-cRcXqjNl9MSzA9xC2yzpSY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16397323</pqid></control><display><type>article</type><title>Sensitized immunochemical method for the specific detection of human haemoglobin in dried biological samples</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Ottó, Szabolcs ; Németh, Mária Sz ; Kocsis, György ; Tulok, István ; Kelemen, Katalin</creator><creatorcontrib>Ottó, Szabolcs ; Németh, Mária Sz ; Kocsis, György ; Tulok, István ; Kelemen, Katalin</creatorcontrib><description>The authors describe a modified version of counterimmunoelectrophoresis of easy performance and high sensitivity. With this method human haemoglobin can easily and specifically be determined either in fresh or dried biological samples. Glutaraldehyde pretreatment of the samples results in the development of complexes with highly favourable electrophoretic mobility and precipitating capacity. By this chemical modification and use of a double set of samples with varying antigen‐antibody, proportion, the sensitivity of haemoglobin detection in erythrocyte‐containing haemolysate and native blood was 300 ng/ml. The examination is easily performed, and without considerable outlay, even with conventional laboratory facilities. It is equally suitable for the detection of occult colorectal bleeding and for species‐specific study of blood stains of unknown origin.
The authors succeeded in identifying human haemoglobin even in a 42‐month dried blood stain.</description><identifier>ISSN: 0887-8013</identifier><identifier>EISSN: 1098-2825</identifier><identifier>DOI: 10.1002/jcla.1860050602</identifier><identifier>PMID: 1774601</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Blood Stains ; Colorectal Neoplasms - diagnosis ; Counterimmunoelectrophoresis - methods ; Counterimmunoelectrophoresis - statistics & numerical data ; dried blood stains ; Evaluation Studies as Topic ; fecal bleeding ; Glutaral ; Hemoglobins - analysis ; Hemoglobins - immunology ; Humans ; Immunochemistry ; Occult Blood ; Sensitivity and Specificity ; sensitized counterimmunoelectrophoresis ; Species Specificity</subject><ispartof>Journal of clinical laboratory analysis, 1991, Vol.5 (6), p.373-377</ispartof><rights>Copyright © 1991 Wiley Periodicals, Inc., A Wiley Company</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4132-38a4363ae7934a2ad719e4a695238202c6744b2c8e60ef6e8d7e2317585ca51a3</citedby><cites>FETCH-LOGICAL-c4132-38a4363ae7934a2ad719e4a695238202c6744b2c8e60ef6e8d7e2317585ca51a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcla.1860050602$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcla.1860050602$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,4014,27914,27915,27916,45565,45566</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1774601$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ottó, Szabolcs</creatorcontrib><creatorcontrib>Németh, Mária Sz</creatorcontrib><creatorcontrib>Kocsis, György</creatorcontrib><creatorcontrib>Tulok, István</creatorcontrib><creatorcontrib>Kelemen, Katalin</creatorcontrib><title>Sensitized immunochemical method for the specific detection of human haemoglobin in dried biological samples</title><title>Journal of clinical laboratory analysis</title><addtitle>J. Clin. Lab. Anal</addtitle><description>The authors describe a modified version of counterimmunoelectrophoresis of easy performance and high sensitivity. With this method human haemoglobin can easily and specifically be determined either in fresh or dried biological samples. Glutaraldehyde pretreatment of the samples results in the development of complexes with highly favourable electrophoretic mobility and precipitating capacity. By this chemical modification and use of a double set of samples with varying antigen‐antibody, proportion, the sensitivity of haemoglobin detection in erythrocyte‐containing haemolysate and native blood was 300 ng/ml. The examination is easily performed, and without considerable outlay, even with conventional laboratory facilities. It is equally suitable for the detection of occult colorectal bleeding and for species‐specific study of blood stains of unknown origin.
The authors succeeded in identifying human haemoglobin even in a 42‐month dried blood stain.</description><subject>Blood Stains</subject><subject>Colorectal Neoplasms - diagnosis</subject><subject>Counterimmunoelectrophoresis - methods</subject><subject>Counterimmunoelectrophoresis - statistics & numerical data</subject><subject>dried blood stains</subject><subject>Evaluation Studies as Topic</subject><subject>fecal bleeding</subject><subject>Glutaral</subject><subject>Hemoglobins - analysis</subject><subject>Hemoglobins - immunology</subject><subject>Humans</subject><subject>Immunochemistry</subject><subject>Occult Blood</subject><subject>Sensitivity and Specificity</subject><subject>sensitized counterimmunoelectrophoresis</subject><subject>Species Specificity</subject><issn>0887-8013</issn><issn>1098-2825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQRi1EVZbCmROST9zSju3EdsSpWkFLtaJFgMrN8jqTxsWOlzgRlF_flFRUPVUaaQ7zvneYj5A3DA4ZAD-6dsEeMi0BKpDAn5EVg1oXXPPqOVmB1qrQwMQL8jLnawDQNZP7ZJ8pVUpgKxK-Yp_96P9iQ32MU59ch9E7G2jEsUsNbdNAxw5p3qHzrXe0wRHd6FNPU0u7KdqedhZjugpp63s6TzP4Wbf1KaSrf6ps4y5gfkX2Whsyvr7fB-T7xw_f1qfF5vzk0_p4U7iSCV4IbUshhUVVi9Jy2yhWY2llXXGhOXAnVVluudMoAVuJulHIBVOVrpytmBUH5N3i3Q3p14R5NNFnhyHYHtOUjeKSs7qsnwSZFLUSXMzg0QK6IeU8YGt2g492uDEMzF0R5q4I81DEnHh7r562EZsHfvn8fH-_3H_7gDdP6czZenP8yF4saZ9H_PM_bYefRiqhKnP5-cRcXqjNl9MSzA9xC2yzpSY</recordid><startdate>1991</startdate><enddate>1991</enddate><creator>Ottó, Szabolcs</creator><creator>Németh, Mária Sz</creator><creator>Kocsis, György</creator><creator>Tulok, István</creator><creator>Kelemen, Katalin</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>1991</creationdate><title>Sensitized immunochemical method for the specific detection of human haemoglobin in dried biological samples</title><author>Ottó, Szabolcs ; Németh, Mária Sz ; Kocsis, György ; Tulok, István ; Kelemen, Katalin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4132-38a4363ae7934a2ad719e4a695238202c6744b2c8e60ef6e8d7e2317585ca51a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Blood Stains</topic><topic>Colorectal Neoplasms - diagnosis</topic><topic>Counterimmunoelectrophoresis - methods</topic><topic>Counterimmunoelectrophoresis - statistics & numerical data</topic><topic>dried blood stains</topic><topic>Evaluation Studies as Topic</topic><topic>fecal bleeding</topic><topic>Glutaral</topic><topic>Hemoglobins - analysis</topic><topic>Hemoglobins - immunology</topic><topic>Humans</topic><topic>Immunochemistry</topic><topic>Occult Blood</topic><topic>Sensitivity and Specificity</topic><topic>sensitized counterimmunoelectrophoresis</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ottó, Szabolcs</creatorcontrib><creatorcontrib>Németh, Mária Sz</creatorcontrib><creatorcontrib>Kocsis, György</creatorcontrib><creatorcontrib>Tulok, István</creatorcontrib><creatorcontrib>Kelemen, Katalin</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of clinical laboratory analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ottó, Szabolcs</au><au>Németh, Mária Sz</au><au>Kocsis, György</au><au>Tulok, István</au><au>Kelemen, Katalin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sensitized immunochemical method for the specific detection of human haemoglobin in dried biological samples</atitle><jtitle>Journal of clinical laboratory analysis</jtitle><addtitle>J. Clin. Lab. Anal</addtitle><date>1991</date><risdate>1991</risdate><volume>5</volume><issue>6</issue><spage>373</spage><epage>377</epage><pages>373-377</pages><issn>0887-8013</issn><eissn>1098-2825</eissn><abstract>The authors describe a modified version of counterimmunoelectrophoresis of easy performance and high sensitivity. With this method human haemoglobin can easily and specifically be determined either in fresh or dried biological samples. Glutaraldehyde pretreatment of the samples results in the development of complexes with highly favourable electrophoretic mobility and precipitating capacity. By this chemical modification and use of a double set of samples with varying antigen‐antibody, proportion, the sensitivity of haemoglobin detection in erythrocyte‐containing haemolysate and native blood was 300 ng/ml. The examination is easily performed, and without considerable outlay, even with conventional laboratory facilities. It is equally suitable for the detection of occult colorectal bleeding and for species‐specific study of blood stains of unknown origin.
The authors succeeded in identifying human haemoglobin even in a 42‐month dried blood stain.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>1774601</pmid><doi>10.1002/jcla.1860050602</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0887-8013 |
| ispartof | Journal of clinical laboratory analysis, 1991, Vol.5 (6), p.373-377 |
| issn | 0887-8013 1098-2825 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72621949 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; EZB-FREE-00999 freely available EZB journals |
| subjects | Blood Stains Colorectal Neoplasms - diagnosis Counterimmunoelectrophoresis - methods Counterimmunoelectrophoresis - statistics & numerical data dried blood stains Evaluation Studies as Topic fecal bleeding Glutaral Hemoglobins - analysis Hemoglobins - immunology Humans Immunochemistry Occult Blood Sensitivity and Specificity sensitized counterimmunoelectrophoresis Species Specificity |
| title | Sensitized immunochemical method for the specific detection of human haemoglobin in dried biological samples |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T22%3A50%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sensitized%20immunochemical%20method%20for%20the%20specific%20detection%20of%20human%20haemoglobin%20in%20dried%20biological%20samples&rft.jtitle=Journal%20of%20clinical%20laboratory%20analysis&rft.au=Ott%C3%B3,%20Szabolcs&rft.date=1991&rft.volume=5&rft.issue=6&rft.spage=373&rft.epage=377&rft.pages=373-377&rft.issn=0887-8013&rft.eissn=1098-2825&rft_id=info:doi/10.1002/jcla.1860050602&rft_dat=%3Cproquest_cross%3E16397323%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16397323&rft_id=info:pmid/1774601&rfr_iscdi=true |