Effect of nucleotides and their analogues on essential light chains in myosin head
The domain movement in myosin head plays a decisive role in the energy transduction process of the muscle contraction. During hydrolysis of ATP, the specific formation of strong binding of myosin head for actin causes conformational changes. As a consequence, the light chain-binding motif generates...
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| Veröffentlicht in: | Journal of biochemical and biophysical methods 2002-10, Vol.53 (1), p.67-74 |
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| creator | Hartvig, Nóra Gaszner, Balázs Kiss, Márta Lőrinczy, Dénes Belágyi, Joseph |
| description | The domain movement in myosin head plays a decisive role in the energy transduction process of the muscle contraction. During hydrolysis of ATP, the specific formation of strong binding of myosin head for actin causes conformational changes. As a consequence, the light chain-binding motif generates the powerstroke. In our work maleimide spin labels were covalently attached to Cys-177 residue of ELC in subfragment-1 (S1). Our goal was to study the orientation dependence and the motion of S1, which were incorporated into glycerinated skeletal muscle fibres. The electron paramagnetic resonance spectroscopy (EPR) spectra of the probes depended strongly on the orientation of the fibre axis relative to the magnetic field, indicating that the essential light chain (ELC) and the neck were ordered. The probes were undergoing rapid motion within a cone. The half-width of the cone was estimated to be 65±5° (SD,
n=8). Addition of ADP affected little the hyperfine splitting and the angular spread of the probe distribution. In the presence of ADP and orthovanadate the intensity of the spectra decreased, which showed the dissociation of S1 and this was accompanied with the disappearance of the orientation dependence. |
| doi_str_mv | 10.1016/S0165-022X(02)00094-5 |
| format | Article |
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n=8). Addition of ADP affected little the hyperfine splitting and the angular spread of the probe distribution. In the presence of ADP and orthovanadate the intensity of the spectra decreased, which showed the dissociation of S1 and this was accompanied with the disappearance of the orientation dependence.</description><subject>Adenosine Diphosphate - chemistry</subject><subject>Adenosine Diphosphate - metabolism</subject><subject>Animals</subject><subject>Computer Simulation</subject><subject>Domain movement</subject><subject>Electron Spin Resonance Spectroscopy - methods</subject><subject>EPR</subject><subject>Essential light chain</subject><subject>In Vitro Techniques</subject><subject>Maleimides</subject><subject>Models, Molecular</subject><subject>Molecular Motor Proteins - chemistry</subject><subject>Molecular Motor Proteins - metabolism</subject><subject>Molecular Probe Techniques</subject><subject>Motion</subject><subject>Muscle Fibers, Skeletal - chemistry</subject><subject>Muscle Fibers, Skeletal - metabolism</subject><subject>Muscle, Skeletal</subject><subject>Myosin Light Chains - chemistry</subject><subject>Myosin Light Chains - metabolism</subject><subject>Myosin Subfragments - chemistry</subject><subject>Myosin Subfragments - metabolism</subject><subject>Myosins - chemistry</subject><subject>Myosins - metabolism</subject><subject>Nucleotides - chemistry</subject><subject>Nucleotides - metabolism</subject><subject>Protein Structure, Tertiary</subject><subject>Psoas Muscles</subject><subject>Rabbits</subject><subject>Spin Labels</subject><subject>Subfragment-1</subject><issn>0165-022X</issn><issn>1872-857X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1LxDAQhoMouq7-BCUn0UM1mSZtehJZ_AJB8AO8hTSZupFuo01X8N-b_UCPepkJ4UnmZR5CDjg75YwXZ4-pyIwBvBwzOGGMVSKTG2TEVQmZkuXLJhn9IDtkN8a3BOUKxDbZ4SBYIZUakYfLpkE70NDQbm5bDIN3GKnpHB2m6Pt0Mm14nae70FGMEbvBm5a2_nU6UDs1vovUd3T2FWJqUzRuj2w1po24v-5j8nx1-TS5ye7ur28nF3eZFZAPmShzsNLUtqpUYUwFjWm4AAmWI6us5cy6FLFB6XgtJDfSCeSmhLxWucE6H5Oj1b_vffhIAQc989Fi25oOwzzqEgrgJcg_QeBcKFXBv0BZFEUC5Qq0fYixx0a_935m-i_NmV7o0Us9erH7VPRSj14kOVwPmNczdL-v1j4ScL4CMC3u02Ovo_XYWXS-T5q0C_6PEd_Dwp-H</recordid><startdate>20021001</startdate><enddate>20021001</enddate><creator>Hartvig, Nóra</creator><creator>Gaszner, Balázs</creator><creator>Kiss, Márta</creator><creator>Lőrinczy, Dénes</creator><creator>Belágyi, Joseph</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20021001</creationdate><title>Effect of nucleotides and their analogues on essential light chains in myosin head</title><author>Hartvig, Nóra ; Gaszner, Balázs ; Kiss, Márta ; Lőrinczy, Dénes ; Belágyi, Joseph</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-4732c5abc9986aa92faf14252c1e09cc10cd588fe5d1b451a5d4e1a723b83aeb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adenosine Diphosphate - chemistry</topic><topic>Adenosine Diphosphate - metabolism</topic><topic>Animals</topic><topic>Computer Simulation</topic><topic>Domain movement</topic><topic>Electron Spin Resonance Spectroscopy - methods</topic><topic>EPR</topic><topic>Essential light chain</topic><topic>In Vitro Techniques</topic><topic>Maleimides</topic><topic>Models, Molecular</topic><topic>Molecular Motor Proteins - chemistry</topic><topic>Molecular Motor Proteins - metabolism</topic><topic>Molecular Probe Techniques</topic><topic>Motion</topic><topic>Muscle Fibers, Skeletal - chemistry</topic><topic>Muscle Fibers, Skeletal - metabolism</topic><topic>Muscle, Skeletal</topic><topic>Myosin Light Chains - chemistry</topic><topic>Myosin Light Chains - metabolism</topic><topic>Myosin Subfragments - chemistry</topic><topic>Myosin Subfragments - metabolism</topic><topic>Myosins - chemistry</topic><topic>Myosins - metabolism</topic><topic>Nucleotides - chemistry</topic><topic>Nucleotides - metabolism</topic><topic>Protein Structure, Tertiary</topic><topic>Psoas Muscles</topic><topic>Rabbits</topic><topic>Spin Labels</topic><topic>Subfragment-1</topic><toplevel>online_resources</toplevel><creatorcontrib>Hartvig, Nóra</creatorcontrib><creatorcontrib>Gaszner, Balázs</creatorcontrib><creatorcontrib>Kiss, Márta</creatorcontrib><creatorcontrib>Lőrinczy, Dénes</creatorcontrib><creatorcontrib>Belágyi, Joseph</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biochemical and biophysical methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hartvig, Nóra</au><au>Gaszner, Balázs</au><au>Kiss, Márta</au><au>Lőrinczy, Dénes</au><au>Belágyi, Joseph</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of nucleotides and their analogues on essential light chains in myosin head</atitle><jtitle>Journal of biochemical and biophysical methods</jtitle><addtitle>J Biochem Biophys Methods</addtitle><date>2002-10-01</date><risdate>2002</risdate><volume>53</volume><issue>1</issue><spage>67</spage><epage>74</epage><pages>67-74</pages><issn>0165-022X</issn><eissn>1872-857X</eissn><abstract>The domain movement in myosin head plays a decisive role in the energy transduction process of the muscle contraction. 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| subjects | Adenosine Diphosphate - chemistry Adenosine Diphosphate - metabolism Animals Computer Simulation Domain movement Electron Spin Resonance Spectroscopy - methods EPR Essential light chain In Vitro Techniques Maleimides Models, Molecular Molecular Motor Proteins - chemistry Molecular Motor Proteins - metabolism Molecular Probe Techniques Motion Muscle Fibers, Skeletal - chemistry Muscle Fibers, Skeletal - metabolism Muscle, Skeletal Myosin Light Chains - chemistry Myosin Light Chains - metabolism Myosin Subfragments - chemistry Myosin Subfragments - metabolism Myosins - chemistry Myosins - metabolism Nucleotides - chemistry Nucleotides - metabolism Protein Structure, Tertiary Psoas Muscles Rabbits Spin Labels Subfragment-1 |
| title | Effect of nucleotides and their analogues on essential light chains in myosin head |
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