Platelet Aggregation by Strains of Enterococci
The platelet aggregation capability of whole cells of Enterococcus faecalis, E. faecium and E. avium was tested. The optimum ratios of bacteria to platelets in E. faecalis (strain SMU-37), E. faecium (strain SMU-138) and E. avium (strain SMU-197) were 1.0, 1.2 and 2.0, respectively. During the plate...
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| Veröffentlicht in: | MICROBIOLOGY and IMMUNOLOGY 1991, Vol.35(11), pp.933-942 |
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| container_title | MICROBIOLOGY and IMMUNOLOGY |
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| creator | Usui, Yukio Ichiman, Yoshitoshi Suganuma, Masaru Yoshida, Kosaku |
| description | The platelet aggregation capability of whole cells of Enterococcus faecalis, E. faecium and E. avium was tested. The optimum ratios of bacteria to platelets in E. faecalis (strain SMU-37), E. faecium (strain SMU-138) and E. avium (strain SMU-197) were 1.0, 1.2 and 2.0, respectively. During the platelet aggregation induced by the three strains of enterococci, 65-69% of total serotonin was released. The aggregation was totally inhibited by ethylenediaminetetraacetate (10mM) and apyrase (1mg/ml), while no effect was shown by aspirin (10mM), indomethacin (10mM) and quinacrine (1mM). By pretreatment of platelet-poor plasma with heat (56C, 30min) or zymosan, the reactivities with platelets of each strain of species were markedly diminished. These results suggest that enterococci-induced platelet aggregation was an ion-dependent, cyclooxygenase-insensitive event, and plasma component(s) was (were) required for the reaction. |
| doi_str_mv | 10.1111/j.1348-0421.1991.tb01615.x |
| format | Article |
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The optimum ratios of bacteria to platelets in E. faecalis (strain SMU-37), E. faecium (strain SMU-138) and E. avium (strain SMU-197) were 1.0, 1.2 and 2.0, respectively. During the platelet aggregation induced by the three strains of enterococci, 65-69% of total serotonin was released. The aggregation was totally inhibited by ethylenediaminetetraacetate (10mM) and apyrase (1mg/ml), while no effect was shown by aspirin (10mM), indomethacin (10mM) and quinacrine (1mM). By pretreatment of platelet-poor plasma with heat (56C, 30min) or zymosan, the reactivities with platelets of each strain of species were markedly diminished. These results suggest that enterococci-induced platelet aggregation was an ion-dependent, cyclooxygenase-insensitive event, and plasma component(s) was (were) required for the reaction.</description><identifier>ISSN: 0385-5600</identifier><identifier>EISSN: 1348-0421</identifier><identifier>DOI: 10.1111/j.1348-0421.1991.tb01615.x</identifier><identifier>PMID: 1775097</identifier><identifier>CODEN: MIIMDV</identifier><language>eng</language><publisher>Tokyo: Blackwell Publishing Ltd</publisher><subject>Animals ; Arachidonic Acid - metabolism ; Bacteriology ; Biological and medical sciences ; Enterococcus - physiology ; Enterococcus avium ; Enterococcus faecalis ; Enterococcus faecium ; Fundamental and applied biological sciences. Psychology ; In Vitro Techniques ; Microbiology ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Plasma - physiology ; Platelet Aggregation - physiology ; Rabbits</subject><ispartof>MICROBIOLOGY and IMMUNOLOGY, 1991, Vol.35(11), pp.933-942</ispartof><rights>Center for Academic Publications Japan</rights><rights>owned by Center for Academic Publications Japan (Publisher)</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6395-f54307c74438aad981bbae5b887b5e010ab99049eb99d0b4f99c25fbd3781c3c3</citedby><cites>FETCH-LOGICAL-c6395-f54307c74438aad981bbae5b887b5e010ab99049eb99d0b4f99c25fbd3781c3c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5218865$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1775097$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Usui, Yukio</creatorcontrib><creatorcontrib>Ichiman, Yoshitoshi</creatorcontrib><creatorcontrib>Suganuma, Masaru</creatorcontrib><creatorcontrib>Yoshida, Kosaku</creatorcontrib><title>Platelet Aggregation by Strains of Enterococci</title><title>MICROBIOLOGY and IMMUNOLOGY</title><addtitle>Microbiology and Immunology</addtitle><description>The platelet aggregation capability of whole cells of Enterococcus faecalis, E. faecium and E. avium was tested. The optimum ratios of bacteria to platelets in E. faecalis (strain SMU-37), E. faecium (strain SMU-138) and E. avium (strain SMU-197) were 1.0, 1.2 and 2.0, respectively. During the platelet aggregation induced by the three strains of enterococci, 65-69% of total serotonin was released. The aggregation was totally inhibited by ethylenediaminetetraacetate (10mM) and apyrase (1mg/ml), while no effect was shown by aspirin (10mM), indomethacin (10mM) and quinacrine (1mM). By pretreatment of platelet-poor plasma with heat (56C, 30min) or zymosan, the reactivities with platelets of each strain of species were markedly diminished. These results suggest that enterococci-induced platelet aggregation was an ion-dependent, cyclooxygenase-insensitive event, and plasma component(s) was (were) required for the reaction.</description><subject>Animals</subject><subject>Arachidonic Acid - metabolism</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Enterococcus - physiology</subject><subject>Enterococcus avium</subject><subject>Enterococcus faecalis</subject><subject>Enterococcus faecium</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In Vitro Techniques</subject><subject>Microbiology</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Plasma - physiology</subject><subject>Platelet Aggregation - physiology</subject><subject>Rabbits</subject><issn>0385-5600</issn><issn>1348-0421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkV1rFDEUhoModa3-BGEQEW9mzOck8aqltNtCqwUVwZuQZDJr1tmZmmTp7r9vxhlWrxRzkQTOc56E9wDwCsEK5fVuXSFCRQkpRhWSElXJQFQjVu0egcWh9BgsIBGsZDWET8GzGNcQYo4FPQJHiHMGJV-A6rbTyXUuFaerVXArnfzQF2ZffEpB-z4WQ1uc98mFwQ7W-ufgSau76F7M5zH4cnH--eyyvP64vDo7vS5tTSQrW0YJ5JZTSoTWjRTIGO2YEYIb5iCC2kgJqXT5aKChrZQWs9Y0hAtkiSXH4M3kvQvDz62LSW18tK7rdO-GbVQc14hgyf8J5lhqgjDN4Nu_g1RmJ6Z0RN9PqA1DjMG16i74jQ57haAaB6DWakxZjSmrcQBqHoDa5eaX8ztbs3HN79Yp8Vx_Pdd1tLprg-6tjweMYSREzTJ2MmH3vnP7__iAurm6-XXNiuWkWMekV-7g0CF52zm10X3jkeRcEZat8y4JORD2uw7K9dlUTiYfk9v9Ifqhak44U18_LNUl_HYr6wupMHkALg3IYw</recordid><startdate>19910101</startdate><enddate>19910101</enddate><creator>Usui, Yukio</creator><creator>Ichiman, Yoshitoshi</creator><creator>Suganuma, Masaru</creator><creator>Yoshida, Kosaku</creator><general>Blackwell Publishing Ltd</general><general>Center For Academic Publications Japan</general><general>Center for Academic Publications Japan</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19910101</creationdate><title>Platelet Aggregation by Strains of Enterococci</title><author>Usui, Yukio ; Ichiman, Yoshitoshi ; Suganuma, Masaru ; Yoshida, Kosaku</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6395-f54307c74438aad981bbae5b887b5e010ab99049eb99d0b4f99c25fbd3781c3c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>Arachidonic Acid - metabolism</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Enterococcus - physiology</topic><topic>Enterococcus avium</topic><topic>Enterococcus faecalis</topic><topic>Enterococcus faecium</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In Vitro Techniques</topic><topic>Microbiology</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Plasma - physiology</topic><topic>Platelet Aggregation - physiology</topic><topic>Rabbits</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Usui, Yukio</creatorcontrib><creatorcontrib>Ichiman, Yoshitoshi</creatorcontrib><creatorcontrib>Suganuma, Masaru</creatorcontrib><creatorcontrib>Yoshida, Kosaku</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>MICROBIOLOGY and IMMUNOLOGY</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Usui, Yukio</au><au>Ichiman, Yoshitoshi</au><au>Suganuma, Masaru</au><au>Yoshida, Kosaku</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Platelet Aggregation by Strains of Enterococci</atitle><jtitle>MICROBIOLOGY and IMMUNOLOGY</jtitle><addtitle>Microbiology and Immunology</addtitle><date>1991-01-01</date><risdate>1991</risdate><volume>35</volume><issue>11</issue><spage>933</spage><epage>942</epage><pages>933-942</pages><issn>0385-5600</issn><eissn>1348-0421</eissn><coden>MIIMDV</coden><abstract>The platelet aggregation capability of whole cells of Enterococcus faecalis, E. faecium and E. avium was tested. The optimum ratios of bacteria to platelets in E. faecalis (strain SMU-37), E. faecium (strain SMU-138) and E. avium (strain SMU-197) were 1.0, 1.2 and 2.0, respectively. During the platelet aggregation induced by the three strains of enterococci, 65-69% of total serotonin was released. The aggregation was totally inhibited by ethylenediaminetetraacetate (10mM) and apyrase (1mg/ml), while no effect was shown by aspirin (10mM), indomethacin (10mM) and quinacrine (1mM). By pretreatment of platelet-poor plasma with heat (56C, 30min) or zymosan, the reactivities with platelets of each strain of species were markedly diminished. These results suggest that enterococci-induced platelet aggregation was an ion-dependent, cyclooxygenase-insensitive event, and plasma component(s) was (were) required for the reaction.</abstract><cop>Tokyo</cop><pub>Blackwell Publishing Ltd</pub><pmid>1775097</pmid><doi>10.1111/j.1348-0421.1991.tb01615.x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| source | J-STAGE Free; MEDLINE; Freely Accessible Japanese Titles; Alma/SFX Local Collection |
| subjects | Animals Arachidonic Acid - metabolism Bacteriology Biological and medical sciences Enterococcus - physiology Enterococcus avium Enterococcus faecalis Enterococcus faecium Fundamental and applied biological sciences. Psychology In Vitro Techniques Microbiology Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Plasma - physiology Platelet Aggregation - physiology Rabbits |
| title | Platelet Aggregation by Strains of Enterococci |
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