Cell-free expression of the coxsackievirus 3C protease using the translational initiation signal of an insect virus RNA and its characterization

We have expressed the 3C protease of coxsackievirus B3 (CVB3) in a cell-free system. This expression system employs the translational initiation signal of an insect virus RNA, black beetle virus (BBV) RNA 1, to direct CVB3-specific protein synthesis. Using this expression system, we demonstrate that...

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Veröffentlicht in:	Virus research 1991-08, Vol.20 (3), p.237-249
Hauptverfasser:	Dasmahapatra, Bimalendu, Rozhon, Edward J., Hart, Andrea M., Cox, Stuart, Tracy, Steven, Schwartz, Jerome
Format:	Artikel
Sprache:	eng
Schlagworte:	3C protease 3C Viral Proteases Base Sequence Biological and medical sciences Blotting, Northern Capsid - biosynthesis Cell free translation Cells, Cultured Chromosome Mapping coxsackievirus Coxsackievirus B3 Cysteine Endopeptidases - analysis Cysteine Endopeptidases - biosynthesis Cysteine Endopeptidases - genetics Enterovirus Fundamental and applied biological sciences. Psychology Gene Expression Genetics HeLa Cells Humans In Vitro Techniques Microbiology Molecular Sequence Data PI processing Plasmids Precipitin Tests Protein Biosynthesis RNA, Viral - genetics Transcription, Genetic Viral Proteins - analysis Viral Proteins - biosynthesis Virology
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