The maternal gene product D7 is not required for early Xenopus development
The Xenopus D7 gene codes for a novel protein whose expression is restricted to early development. D7 protein is synthetized for the first time during oocyte maturation (1988, Genes Dev. 2, 1296–1306). Injection of D7 RNA into the full-grown oocyte and its subsequent translation into D7 protein neit...
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| Veröffentlicht in: | Mechanisms of development 1991-11, Vol.35 (3), p.213-225 |
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| creator | Smith, Rosamund C. Dworkin, Mark B. Dworkin-Rastl, Eva |
| description | The
Xenopus D7 gene codes for a novel protein whose expression is restricted to early development. D7 protein is synthetized for the first time during oocyte maturation (1988, Genes Dev. 2, 1296–1306). Injection of D7 RNA into the full-grown oocyte and its subsequent translation into D7 protein neither induced oocyte maturation nor affected the kinetics of hormone-induced maturation. Overexpression of D7 protein by 20-fold in the early
Xenopus embryo by injection of D7 RNA into fertilized eggs did not affect subsequent development. Oocytes specifically lacking D7 mRNA were generated by oligodeoxynucleotide-mediated RNA destruction within the oocyte. Unfertilized eggs generated from such oocytes lacked detectable D7 protein, but nevertheless could be activated and fertilized. Embryos generated from such eggs, estimated to contain less than 5% of wildtype levels of D7 protein, developed normally up to the tailbud stage. Thus the D7 protein, the product of a maternal mRNA that is under strict translational repression in oocytes, appears not to be required for oocyte maturation, activation, fertilization or early embryonic development in
Xenopus. |
| doi_str_mv | 10.1016/0925-4773(91)90020-7 |
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Xenopus D7 gene codes for a novel protein whose expression is restricted to early development. D7 protein is synthetized for the first time during oocyte maturation (1988, Genes Dev. 2, 1296–1306). Injection of D7 RNA into the full-grown oocyte and its subsequent translation into D7 protein neither induced oocyte maturation nor affected the kinetics of hormone-induced maturation. Overexpression of D7 protein by 20-fold in the early
Xenopus embryo by injection of D7 RNA into fertilized eggs did not affect subsequent development. Oocytes specifically lacking D7 mRNA were generated by oligodeoxynucleotide-mediated RNA destruction within the oocyte. Unfertilized eggs generated from such oocytes lacked detectable D7 protein, but nevertheless could be activated and fertilized. Embryos generated from such eggs, estimated to contain less than 5% of wildtype levels of D7 protein, developed normally up to the tailbud stage. Thus the D7 protein, the product of a maternal mRNA that is under strict translational repression in oocytes, appears not to be required for oocyte maturation, activation, fertilization or early embryonic development in
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Xenopus D7 gene codes for a novel protein whose expression is restricted to early development. D7 protein is synthetized for the first time during oocyte maturation (1988, Genes Dev. 2, 1296–1306). Injection of D7 RNA into the full-grown oocyte and its subsequent translation into D7 protein neither induced oocyte maturation nor affected the kinetics of hormone-induced maturation. Overexpression of D7 protein by 20-fold in the early
Xenopus embryo by injection of D7 RNA into fertilized eggs did not affect subsequent development. Oocytes specifically lacking D7 mRNA were generated by oligodeoxynucleotide-mediated RNA destruction within the oocyte. Unfertilized eggs generated from such oocytes lacked detectable D7 protein, but nevertheless could be activated and fertilized. Embryos generated from such eggs, estimated to contain less than 5% of wildtype levels of D7 protein, developed normally up to the tailbud stage. Thus the D7 protein, the product of a maternal mRNA that is under strict translational repression in oocytes, appears not to be required for oocyte maturation, activation, fertilization or early embryonic development in
Xenopus.</description><subject>Animals</subject><subject>Antisense Elements (Genetics)</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Egg Proteins - genetics</subject><subject>Embryo, Nonmammalian - metabolism</subject><subject>Embryogenesis</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Fertilization</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>General aspects</subject><subject>General aspects. Development. Fetal membranes</subject><subject>Maternal mRNA</subject><subject>Molecular Sequence Data</subject><subject>Oligodeoxyribonucleotide</subject><subject>Oligodeoxyribonucleotides</subject><subject>Oocyte maturation</subject><subject>Oocytes - cytology</subject><subject>Oocytes - metabolism</subject><subject>Oogenesis</subject><subject>Translational control</subject><subject>Xenopus laevis</subject><issn>0925-4773</issn><issn>1872-6356</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKAzEUhoMotVbfQCELEV2M5jIzmWwEqXcKbiq4C5nMiY7MpU1mCn17U6fUnasD5__-w-FD6JSSa0poekMkS6JYCH4p6ZUkhJFI7KExzQSLUp6k-2i8Qw7RkfffhBBKUzpCIyrSLGVsjF7nX4Br3YFrdIU_oQG8cG3Rmw7fC1x63LQddrDsSwcFtq3DoF21xh_QtIve4wJWULWLGpruGB1YXXk42c4Jen98mE-fo9nb08v0bhYZnqVdZKy0xmbEFNICMCu05pbkVlBruMyFoFmSZ7mE3PJYhjXYEGQ8YdwALWI-QRfD3fDosgffqbr0BqpKN9D2XgmWEhYnNIDxABrXeu_AqoUra-3WihK1Uag2ftTGj5JU_SpUItTOtvf7vIbirzQ4C_n5Ntfe6Mo63ZjS77CExTQjPGC3AwbBxaoEp7wpoTFQBJWmU0Vb_v_HD2vVjaQ</recordid><startdate>19911101</startdate><enddate>19911101</enddate><creator>Smith, Rosamund C.</creator><creator>Dworkin, Mark B.</creator><creator>Dworkin-Rastl, Eva</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19911101</creationdate><title>The maternal gene product D7 is not required for early Xenopus development</title><author>Smith, Rosamund C. ; Dworkin, Mark B. ; Dworkin-Rastl, Eva</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-cf9fcf80cd9fee2f7aa3f0bf71fc39b77185b8b9ebf349f71effc383523ce1d43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>Antisense Elements (Genetics)</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Egg Proteins - genetics</topic><topic>Embryo, Nonmammalian - metabolism</topic><topic>Embryogenesis</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Fertilization</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation</topic><topic>General aspects</topic><topic>General aspects. Development. Fetal membranes</topic><topic>Maternal mRNA</topic><topic>Molecular Sequence Data</topic><topic>Oligodeoxyribonucleotide</topic><topic>Oligodeoxyribonucleotides</topic><topic>Oocyte maturation</topic><topic>Oocytes - cytology</topic><topic>Oocytes - metabolism</topic><topic>Oogenesis</topic><topic>Translational control</topic><topic>Xenopus laevis</topic><toplevel>online_resources</toplevel><creatorcontrib>Smith, Rosamund C.</creatorcontrib><creatorcontrib>Dworkin, Mark B.</creatorcontrib><creatorcontrib>Dworkin-Rastl, Eva</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Mechanisms of development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Smith, Rosamund C.</au><au>Dworkin, Mark B.</au><au>Dworkin-Rastl, Eva</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The maternal gene product D7 is not required for early Xenopus development</atitle><jtitle>Mechanisms of development</jtitle><addtitle>Mech Dev</addtitle><date>1991-11-01</date><risdate>1991</risdate><volume>35</volume><issue>3</issue><spage>213</spage><epage>225</epage><pages>213-225</pages><issn>0925-4773</issn><eissn>1872-6356</eissn><abstract>The
Xenopus D7 gene codes for a novel protein whose expression is restricted to early development. D7 protein is synthetized for the first time during oocyte maturation (1988, Genes Dev. 2, 1296–1306). Injection of D7 RNA into the full-grown oocyte and its subsequent translation into D7 protein neither induced oocyte maturation nor affected the kinetics of hormone-induced maturation. Overexpression of D7 protein by 20-fold in the early
Xenopus embryo by injection of D7 RNA into fertilized eggs did not affect subsequent development. Oocytes specifically lacking D7 mRNA were generated by oligodeoxynucleotide-mediated RNA destruction within the oocyte. Unfertilized eggs generated from such oocytes lacked detectable D7 protein, but nevertheless could be activated and fertilized. Embryos generated from such eggs, estimated to contain less than 5% of wildtype levels of D7 protein, developed normally up to the tailbud stage. Thus the D7 protein, the product of a maternal mRNA that is under strict translational repression in oocytes, appears not to be required for oocyte maturation, activation, fertilization or early embryonic development in
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| ispartof | Mechanisms of development, 1991-11, Vol.35 (3), p.213-225 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Antisense Elements (Genetics) Base Sequence Biological and medical sciences Cells, Cultured Egg Proteins - genetics Embryo, Nonmammalian - metabolism Embryogenesis Embryology: invertebrates and vertebrates. Teratology Fertilization Fundamental and applied biological sciences. Psychology Gene Expression Regulation General aspects General aspects. Development. Fetal membranes Maternal mRNA Molecular Sequence Data Oligodeoxyribonucleotide Oligodeoxyribonucleotides Oocyte maturation Oocytes - cytology Oocytes - metabolism Oogenesis Translational control Xenopus laevis |
| title | The maternal gene product D7 is not required for early Xenopus development |
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