Maintenance of human islets in long term culture

The long‐term maintenance of human islets in culture has remained a challenge. Despite advancements in culture techniques, human islets proved to have a short life span in vitro. For the first time, we have succeeded in maintaining human islets in a defined culture medium for more than 12 months. Fr...

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Veröffentlicht in:	Differentiation (London) 2000-12, Vol.66 (4‐5), p.173-180
Hauptverfasser:	Schmied, B.M., Ulrich, A., Matsuzaki, H., Batra, S.K., Pour, P.M., Ding, X., Adrian, T.E., Ricordi, C., Moyer, M.P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Animal cells Biological and medical sciences Biotechnology Cell Differentiation Cell differentiation, maturation, development, hematopoiesis Cell physiology Cell Size culture Cytological Techniques - methods differentiation Establishment of new cell lines, improvement of cultural methods, mass cultures Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology human Humans Insulin - metabolism Insulin Secretion islet Islets of Langerhans - cytology Islets of Langerhans - metabolism Male Methods. Procedures. Technologies Microscopy, Electron Molecular and cellular biology pancreas Somatostatin - metabolism Time Factors
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