An improved intravesical model using human bladder cancer cell lines to optimize gene and other therapies
Orthotopic implantation of human bladder cancer cells into immunodeficient mice is an important tool for studying the biology and effects of therapy. Nevertheless, the incidence of tumor implantation and growth by transurethral instillation of the human bladder cancer cells into murine bladders has...
Gespeichert in:
| Veröffentlicht in: | Cancer gene therapy 2000-12, Vol.7 (12), p.1575-1580 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1580 |
|---|---|
| container_issue | 12 |
| container_start_page | 1575 |
| container_title | Cancer gene therapy |
| container_volume | 7 |
| creator | Watanabe, T Shinohara, N Sazawa, A Harabayashi, T Ogiso, Y Koyanagi, T Takiguchi, M Hashimoto, A Kuzumaki, N Yamashita, M Tanaka, M Grossman, H B Benedict, W F |
| description | Orthotopic implantation of human bladder cancer cells into immunodeficient mice is an important tool for studying the biology and effects of therapy. Nevertheless, the incidence of tumor implantation and growth by transurethral instillation of the human bladder cancer cells into murine bladders has been low or not reproducible. However, using a modified intravesical technique and the human bladder cancer cell lines, KU-7 and UM-UC-2, we have been able to obtain a high and reproducible incidence of superficial bladder tumors. Furthermore, intravesical administration of the LacZ adenovirus vector resulted in significant beta-galactosidase expression in these bladder tumors as well as the normal urothelium, which was associated with the removal of the glycosoaminoglycan layer. Because this modified technique produces a high incidence of superficial human tumor growth and allows the efficacy of gene transfer to be evaluated, it should be a useful model for the study of intravesical gene therapy for human bladder cancer. |
| doi_str_mv | 10.1038/sj.cgt.7700261 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72598881</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>998133451</sourcerecordid><originalsourceid>FETCH-LOGICAL-c417t-d0c1f17be7c983862daa1d3ac612b67cac5678fa76904d6e833209c72750b73b3</originalsourceid><addsrcrecordid>eNqF0U1r3DAQBmBRGppN2muPRbSQm7caaa2RjiH0CwK5pGchS7MbLba8texA8-ujJQuFQulFc9AzwwwvY-9BrEEo87ns12E3rxGFkBpesRVsUDdtK8RrthJW2gasUOfsopS9EPUT1Rt2DiClaZVesXSdeRoO0_hIkac8T_6RSgq-58MYqedLSXnHH5bBZ971PkaaePA5HAv1Pe9TpsLnkY-HOQ3pifiOMnGfIx_nh6qOjz8kKm_Z2db3hd6d6iX7-fXL_c335vbu24-b69smbADnJooAW8COMFijjJbRe4jKBw2y0xh8aDWarUdtxSZqMkpJYQNKbEWHqlOX7Oplbj3q10JldkMqx119pnEpDmVrjTHwXwhohQWNFX76C-7HZcr1CCd1XRqUMrKqj_9UgBsrpNQVrV9QmMZSJtq6w5QGP_12INwxUFf2rgbqToHWhg-nqUs3UPzDTwmqZ5rzm98</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>217490226</pqid></control><display><type>article</type><title>An improved intravesical model using human bladder cancer cell lines to optimize gene and other therapies</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Watanabe, T ; Shinohara, N ; Sazawa, A ; Harabayashi, T ; Ogiso, Y ; Koyanagi, T ; Takiguchi, M ; Hashimoto, A ; Kuzumaki, N ; Yamashita, M ; Tanaka, M ; Grossman, H B ; Benedict, W F</creator><creatorcontrib>Watanabe, T ; Shinohara, N ; Sazawa, A ; Harabayashi, T ; Ogiso, Y ; Koyanagi, T ; Takiguchi, M ; Hashimoto, A ; Kuzumaki, N ; Yamashita, M ; Tanaka, M ; Grossman, H B ; Benedict, W F</creatorcontrib><description>Orthotopic implantation of human bladder cancer cells into immunodeficient mice is an important tool for studying the biology and effects of therapy. Nevertheless, the incidence of tumor implantation and growth by transurethral instillation of the human bladder cancer cells into murine bladders has been low or not reproducible. However, using a modified intravesical technique and the human bladder cancer cell lines, KU-7 and UM-UC-2, we have been able to obtain a high and reproducible incidence of superficial bladder tumors. Furthermore, intravesical administration of the LacZ adenovirus vector resulted in significant beta-galactosidase expression in these bladder tumors as well as the normal urothelium, which was associated with the removal of the glycosoaminoglycan layer. Because this modified technique produces a high incidence of superficial human tumor growth and allows the efficacy of gene transfer to be evaluated, it should be a useful model for the study of intravesical gene therapy for human bladder cancer.</description><identifier>ISSN: 0929-1903</identifier><identifier>EISSN: 1476-5500</identifier><identifier>DOI: 10.1038/sj.cgt.7700261</identifier><identifier>PMID: 11228536</identifier><language>eng</language><publisher>England: Nature Publishing Group</publisher><subject>Adenoviridae - drug effects ; Adenoviridae - genetics ; Adenovirus ; Administration, Intravesical ; Animals ; beta-Galactosidase - genetics ; beta-Galactosidase - metabolism ; Bladder cancer ; Cancer ; Disease Models, Animal ; Gene therapy ; Gene Transfer Techniques ; Genetic Therapy - methods ; Humans ; Immunodeficiency ; Lac Operon - genetics ; Lac Operon - physiology ; Mice ; Mice, Nude ; Tumor cell lines ; Tumor Cells, Cultured - cytology ; Tumor Cells, Cultured - metabolism ; Tumor Cells, Cultured - transplantation ; Tumors ; Urinary Bladder - cytology ; Urinary Bladder - metabolism ; Urinary Bladder Neoplasms - genetics ; Urinary Bladder Neoplasms - pathology ; Urinary Bladder Neoplasms - therapy ; Urothelium ; β-Galactosidase</subject><ispartof>Cancer gene therapy, 2000-12, Vol.7 (12), p.1575-1580</ispartof><rights>Copyright Nature Publishing Group Dec 2000</rights><rights>Nature America, Inc. 2000.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-d0c1f17be7c983862daa1d3ac612b67cac5678fa76904d6e833209c72750b73b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11228536$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Watanabe, T</creatorcontrib><creatorcontrib>Shinohara, N</creatorcontrib><creatorcontrib>Sazawa, A</creatorcontrib><creatorcontrib>Harabayashi, T</creatorcontrib><creatorcontrib>Ogiso, Y</creatorcontrib><creatorcontrib>Koyanagi, T</creatorcontrib><creatorcontrib>Takiguchi, M</creatorcontrib><creatorcontrib>Hashimoto, A</creatorcontrib><creatorcontrib>Kuzumaki, N</creatorcontrib><creatorcontrib>Yamashita, M</creatorcontrib><creatorcontrib>Tanaka, M</creatorcontrib><creatorcontrib>Grossman, H B</creatorcontrib><creatorcontrib>Benedict, W F</creatorcontrib><title>An improved intravesical model using human bladder cancer cell lines to optimize gene and other therapies</title><title>Cancer gene therapy</title><addtitle>Cancer Gene Ther</addtitle><description>Orthotopic implantation of human bladder cancer cells into immunodeficient mice is an important tool for studying the biology and effects of therapy. Nevertheless, the incidence of tumor implantation and growth by transurethral instillation of the human bladder cancer cells into murine bladders has been low or not reproducible. However, using a modified intravesical technique and the human bladder cancer cell lines, KU-7 and UM-UC-2, we have been able to obtain a high and reproducible incidence of superficial bladder tumors. Furthermore, intravesical administration of the LacZ adenovirus vector resulted in significant beta-galactosidase expression in these bladder tumors as well as the normal urothelium, which was associated with the removal of the glycosoaminoglycan layer. Because this modified technique produces a high incidence of superficial human tumor growth and allows the efficacy of gene transfer to be evaluated, it should be a useful model for the study of intravesical gene therapy for human bladder cancer.</description><subject>Adenoviridae - drug effects</subject><subject>Adenoviridae - genetics</subject><subject>Adenovirus</subject><subject>Administration, Intravesical</subject><subject>Animals</subject><subject>beta-Galactosidase - genetics</subject><subject>beta-Galactosidase - metabolism</subject><subject>Bladder cancer</subject><subject>Cancer</subject><subject>Disease Models, Animal</subject><subject>Gene therapy</subject><subject>Gene Transfer Techniques</subject><subject>Genetic Therapy - methods</subject><subject>Humans</subject><subject>Immunodeficiency</subject><subject>Lac Operon - genetics</subject><subject>Lac Operon - physiology</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Tumor cell lines</subject><subject>Tumor Cells, Cultured - cytology</subject><subject>Tumor Cells, Cultured - metabolism</subject><subject>Tumor Cells, Cultured - transplantation</subject><subject>Tumors</subject><subject>Urinary Bladder - cytology</subject><subject>Urinary Bladder - metabolism</subject><subject>Urinary Bladder Neoplasms - genetics</subject><subject>Urinary Bladder Neoplasms - pathology</subject><subject>Urinary Bladder Neoplasms - therapy</subject><subject>Urothelium</subject><subject>β-Galactosidase</subject><issn>0929-1903</issn><issn>1476-5500</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqF0U1r3DAQBmBRGppN2muPRbSQm7caaa2RjiH0CwK5pGchS7MbLba8texA8-ujJQuFQulFc9AzwwwvY-9BrEEo87ns12E3rxGFkBpesRVsUDdtK8RrthJW2gasUOfsopS9EPUT1Rt2DiClaZVesXSdeRoO0_hIkac8T_6RSgq-58MYqedLSXnHH5bBZ971PkaaePA5HAv1Pe9TpsLnkY-HOQ3pifiOMnGfIx_nh6qOjz8kKm_Z2db3hd6d6iX7-fXL_c335vbu24-b69smbADnJooAW8COMFijjJbRe4jKBw2y0xh8aDWarUdtxSZqMkpJYQNKbEWHqlOX7Oplbj3q10JldkMqx119pnEpDmVrjTHwXwhohQWNFX76C-7HZcr1CCd1XRqUMrKqj_9UgBsrpNQVrV9QmMZSJtq6w5QGP_12INwxUFf2rgbqToHWhg-nqUs3UPzDTwmqZ5rzm98</recordid><startdate>20001201</startdate><enddate>20001201</enddate><creator>Watanabe, T</creator><creator>Shinohara, N</creator><creator>Sazawa, A</creator><creator>Harabayashi, T</creator><creator>Ogiso, Y</creator><creator>Koyanagi, T</creator><creator>Takiguchi, M</creator><creator>Hashimoto, A</creator><creator>Kuzumaki, N</creator><creator>Yamashita, M</creator><creator>Tanaka, M</creator><creator>Grossman, H B</creator><creator>Benedict, W F</creator><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>20001201</creationdate><title>An improved intravesical model using human bladder cancer cell lines to optimize gene and other therapies</title><author>Watanabe, T ; Shinohara, N ; Sazawa, A ; Harabayashi, T ; Ogiso, Y ; Koyanagi, T ; Takiguchi, M ; Hashimoto, A ; Kuzumaki, N ; Yamashita, M ; Tanaka, M ; Grossman, H B ; Benedict, W F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-d0c1f17be7c983862daa1d3ac612b67cac5678fa76904d6e833209c72750b73b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adenoviridae - drug effects</topic><topic>Adenoviridae - genetics</topic><topic>Adenovirus</topic><topic>Administration, Intravesical</topic><topic>Animals</topic><topic>beta-Galactosidase - genetics</topic><topic>beta-Galactosidase - metabolism</topic><topic>Bladder cancer</topic><topic>Cancer</topic><topic>Disease Models, Animal</topic><topic>Gene therapy</topic><topic>Gene Transfer Techniques</topic><topic>Genetic Therapy - methods</topic><topic>Humans</topic><topic>Immunodeficiency</topic><topic>Lac Operon - genetics</topic><topic>Lac Operon - physiology</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Tumor cell lines</topic><topic>Tumor Cells, Cultured - cytology</topic><topic>Tumor Cells, Cultured - metabolism</topic><topic>Tumor Cells, Cultured - transplantation</topic><topic>Tumors</topic><topic>Urinary Bladder - cytology</topic><topic>Urinary Bladder - metabolism</topic><topic>Urinary Bladder Neoplasms - genetics</topic><topic>Urinary Bladder Neoplasms - pathology</topic><topic>Urinary Bladder Neoplasms - therapy</topic><topic>Urothelium</topic><topic>β-Galactosidase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Watanabe, T</creatorcontrib><creatorcontrib>Shinohara, N</creatorcontrib><creatorcontrib>Sazawa, A</creatorcontrib><creatorcontrib>Harabayashi, T</creatorcontrib><creatorcontrib>Ogiso, Y</creatorcontrib><creatorcontrib>Koyanagi, T</creatorcontrib><creatorcontrib>Takiguchi, M</creatorcontrib><creatorcontrib>Hashimoto, A</creatorcontrib><creatorcontrib>Kuzumaki, N</creatorcontrib><creatorcontrib>Yamashita, M</creatorcontrib><creatorcontrib>Tanaka, M</creatorcontrib><creatorcontrib>Grossman, H B</creatorcontrib><creatorcontrib>Benedict, W F</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer gene therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Watanabe, T</au><au>Shinohara, N</au><au>Sazawa, A</au><au>Harabayashi, T</au><au>Ogiso, Y</au><au>Koyanagi, T</au><au>Takiguchi, M</au><au>Hashimoto, A</au><au>Kuzumaki, N</au><au>Yamashita, M</au><au>Tanaka, M</au><au>Grossman, H B</au><au>Benedict, W F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An improved intravesical model using human bladder cancer cell lines to optimize gene and other therapies</atitle><jtitle>Cancer gene therapy</jtitle><addtitle>Cancer Gene Ther</addtitle><date>2000-12-01</date><risdate>2000</risdate><volume>7</volume><issue>12</issue><spage>1575</spage><epage>1580</epage><pages>1575-1580</pages><issn>0929-1903</issn><eissn>1476-5500</eissn><abstract>Orthotopic implantation of human bladder cancer cells into immunodeficient mice is an important tool for studying the biology and effects of therapy. Nevertheless, the incidence of tumor implantation and growth by transurethral instillation of the human bladder cancer cells into murine bladders has been low or not reproducible. However, using a modified intravesical technique and the human bladder cancer cell lines, KU-7 and UM-UC-2, we have been able to obtain a high and reproducible incidence of superficial bladder tumors. Furthermore, intravesical administration of the LacZ adenovirus vector resulted in significant beta-galactosidase expression in these bladder tumors as well as the normal urothelium, which was associated with the removal of the glycosoaminoglycan layer. Because this modified technique produces a high incidence of superficial human tumor growth and allows the efficacy of gene transfer to be evaluated, it should be a useful model for the study of intravesical gene therapy for human bladder cancer.</abstract><cop>England</cop><pub>Nature Publishing Group</pub><pmid>11228536</pmid><doi>10.1038/sj.cgt.7700261</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0929-1903 |
| ispartof | Cancer gene therapy, 2000-12, Vol.7 (12), p.1575-1580 |
| issn | 0929-1903 1476-5500 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72598881 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adenoviridae - drug effects Adenoviridae - genetics Adenovirus Administration, Intravesical Animals beta-Galactosidase - genetics beta-Galactosidase - metabolism Bladder cancer Cancer Disease Models, Animal Gene therapy Gene Transfer Techniques Genetic Therapy - methods Humans Immunodeficiency Lac Operon - genetics Lac Operon - physiology Mice Mice, Nude Tumor cell lines Tumor Cells, Cultured - cytology Tumor Cells, Cultured - metabolism Tumor Cells, Cultured - transplantation Tumors Urinary Bladder - cytology Urinary Bladder - metabolism Urinary Bladder Neoplasms - genetics Urinary Bladder Neoplasms - pathology Urinary Bladder Neoplasms - therapy Urothelium β-Galactosidase |
| title | An improved intravesical model using human bladder cancer cell lines to optimize gene and other therapies |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T20%3A41%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=An%20improved%20intravesical%20model%20using%20human%20bladder%20cancer%20cell%20lines%20to%20optimize%20gene%20and%20other%20therapies&rft.jtitle=Cancer%20gene%20therapy&rft.au=Watanabe,%20T&rft.date=2000-12-01&rft.volume=7&rft.issue=12&rft.spage=1575&rft.epage=1580&rft.pages=1575-1580&rft.issn=0929-1903&rft.eissn=1476-5500&rft_id=info:doi/10.1038/sj.cgt.7700261&rft_dat=%3Cproquest_cross%3E998133451%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=217490226&rft_id=info:pmid/11228536&rfr_iscdi=true |