Amperometric measurement of copper ions with a deputy substrate using a novel Saccharomyces cerevisiae sensor
The first microbial biosensor to detect Cu 2+ by an amperometric method has been developed. For this purpose, recombinant Saccharomyces cerevisiae strains are suitable as the microbial component. These strains contain plasmids with the Cu 2+-inducible promoter of the CUP1-gene from Saccharomyces cer...
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| Veröffentlicht in: | Biosensors & bioelectronics 2000-06, Vol.15 (3), p.211-219 |
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| creator | Lehmann, Matthias Riedel, Klaus Adler, Klaus Kunze, Gotthard |
| description | The first microbial biosensor to detect Cu
2+ by an amperometric method has been developed. For this purpose, recombinant
Saccharomyces cerevisiae strains are suitable as the microbial component. These strains contain plasmids with the Cu
2+-inducible promoter of the
CUP1-gene from
Saccharomyces cerevisiae fused to the
lacZ-gene from
E. coli. On this sensor the
CUP1 promoter is first induced by the Cu
2+-containing probe and subsequently lactose is used as a deputy substrate to make the measurement. If Cu
2+ is present in the sample, these recombinant strains are able to utilize lactose as a carbon source, which leads to alterations in the oxygen consumption of the cells. The sensor measured Cu
2+ in a concentration range between 0.5 and 2 mM CuSO
4. In addition, an indirect amperometric measurement principle was developed which allows the detection of samples containing Cu
2+ and fast biodegradable substances. |
| doi_str_mv | 10.1016/S0956-5663(00)00060-9 |
| format | Article |
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2+ by an amperometric method has been developed. For this purpose, recombinant
Saccharomyces cerevisiae strains are suitable as the microbial component. These strains contain plasmids with the Cu
2+-inducible promoter of the
CUP1-gene from
Saccharomyces cerevisiae fused to the
lacZ-gene from
E. coli. On this sensor the
CUP1 promoter is first induced by the Cu
2+-containing probe and subsequently lactose is used as a deputy substrate to make the measurement. If Cu
2+ is present in the sample, these recombinant strains are able to utilize lactose as a carbon source, which leads to alterations in the oxygen consumption of the cells. The sensor measured Cu
2+ in a concentration range between 0.5 and 2 mM CuSO
4. In addition, an indirect amperometric measurement principle was developed which allows the detection of samples containing Cu
2+ and fast biodegradable substances.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/S0956-5663(00)00060-9</identifier><identifier>PMID: 11286339</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Base Sequence ; Biological and medical sciences ; Biosensing Techniques - methods ; Biosensors ; Biotechnology ; Carrier Proteins ; Cells, Immobilized ; Copper - analysis ; CUP1 promoter ; Escherichia coli - genetics ; Fundamental and applied biological sciences. Psychology ; Genes, Fungal ; Heavy metal ions ; Lac Operon ; Lactose - metabolism ; lacZ-gene ; Metallothionein - genetics ; Metallothionein - metabolism ; Methods. Procedures. Technologies ; Microbial sensor ; Microscopy, Electron, Scanning ; Plasmids - genetics ; Promoter Regions, Genetic ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism ; Saccharomycescerevisiae ; Substrate Specificity ; Various methods and equipments</subject><ispartof>Biosensors & bioelectronics, 2000-06, Vol.15 (3), p.211-219</ispartof><rights>2000 Elsevier Science S.A.</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-fa86d5a1cad718be466106a9f1a3a7e8acdf0ff9cf5d895c3a4372ebf38d8b173</citedby><cites>FETCH-LOGICAL-c421t-fa86d5a1cad718be466106a9f1a3a7e8acdf0ff9cf5d895c3a4372ebf38d8b173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0956566300000609$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1424201$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11286339$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lehmann, Matthias</creatorcontrib><creatorcontrib>Riedel, Klaus</creatorcontrib><creatorcontrib>Adler, Klaus</creatorcontrib><creatorcontrib>Kunze, Gotthard</creatorcontrib><title>Amperometric measurement of copper ions with a deputy substrate using a novel Saccharomyces cerevisiae sensor</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>The first microbial biosensor to detect Cu
2+ by an amperometric method has been developed. For this purpose, recombinant
Saccharomyces cerevisiae strains are suitable as the microbial component. These strains contain plasmids with the Cu
2+-inducible promoter of the
CUP1-gene from
Saccharomyces cerevisiae fused to the
lacZ-gene from
E. coli. On this sensor the
CUP1 promoter is first induced by the Cu
2+-containing probe and subsequently lactose is used as a deputy substrate to make the measurement. If Cu
2+ is present in the sample, these recombinant strains are able to utilize lactose as a carbon source, which leads to alterations in the oxygen consumption of the cells. The sensor measured Cu
2+ in a concentration range between 0.5 and 2 mM CuSO
4. In addition, an indirect amperometric measurement principle was developed which allows the detection of samples containing Cu
2+ and fast biodegradable substances.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Carrier Proteins</subject><subject>Cells, Immobilized</subject><subject>Copper - analysis</subject><subject>CUP1 promoter</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Fungal</subject><subject>Heavy metal ions</subject><subject>Lac Operon</subject><subject>Lactose - metabolism</subject><subject>lacZ-gene</subject><subject>Metallothionein - genetics</subject><subject>Metallothionein - metabolism</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbial sensor</subject><subject>Microscopy, Electron, Scanning</subject><subject>Plasmids - genetics</subject><subject>Promoter Regions, Genetic</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomycescerevisiae</subject><subject>Substrate Specificity</subject><subject>Various methods and equipments</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U1v1DAQBmALgehS-AkgHxCCQ8COE8c5oariS6rEoXC2JvaYGiVx8CSL9t_jdlf02JMP88yMNS9jL6V4L4XUH65F3-qq1Vq9FeKdEEKLqn_EdtJ0qmpq1T5mu__kjD0j-l1QJ3vxlJ1JWRutVL9j08W0YE4Trjk6PiHQlnHCeeUpcJeWUuQxzcT_xvWGA_e4bOuB0zbQmmFFvlGcf5XCnPY48mtw7gbKvIND4g4z7iNFQE44U8rP2ZMAI-GL03vOfn7-9OPya3X1_cu3y4uryjW1XKsARvsWpAPfSTNgo7UUGvogQUGHBpwPIoTehdabvnUKGtXVOARlvBlkp87Zm-PcJac_G9Jqp0gOxxFmTBvZrm57I03zIKy7tihjCmyP0OVElDHYJccJ8sFKYW8DsXeB2NtrWyHsXSC2L32vTgu2YUJ_33VKoIDXJwDkYAwZZhfp3jV1UwtZ2Mcjw3K2fcRsyUWcHfqY0a3Wp_jAT_4BacaqJA</recordid><startdate>20000601</startdate><enddate>20000601</enddate><creator>Lehmann, Matthias</creator><creator>Riedel, Klaus</creator><creator>Adler, Klaus</creator><creator>Kunze, Gotthard</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20000601</creationdate><title>Amperometric measurement of copper ions with a deputy substrate using a novel Saccharomyces cerevisiae sensor</title><author>Lehmann, Matthias ; Riedel, Klaus ; Adler, Klaus ; Kunze, Gotthard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c421t-fa86d5a1cad718be466106a9f1a3a7e8acdf0ff9cf5d895c3a4372ebf38d8b173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Carrier Proteins</topic><topic>Cells, Immobilized</topic><topic>Copper - analysis</topic><topic>CUP1 promoter</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Fungal</topic><topic>Heavy metal ions</topic><topic>Lac Operon</topic><topic>Lactose - metabolism</topic><topic>lacZ-gene</topic><topic>Metallothionein - genetics</topic><topic>Metallothionein - metabolism</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbial sensor</topic><topic>Microscopy, Electron, Scanning</topic><topic>Plasmids - genetics</topic><topic>Promoter Regions, Genetic</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Saccharomycescerevisiae</topic><topic>Substrate Specificity</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lehmann, Matthias</creatorcontrib><creatorcontrib>Riedel, Klaus</creatorcontrib><creatorcontrib>Adler, Klaus</creatorcontrib><creatorcontrib>Kunze, Gotthard</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lehmann, Matthias</au><au>Riedel, Klaus</au><au>Adler, Klaus</au><au>Kunze, Gotthard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Amperometric measurement of copper ions with a deputy substrate using a novel Saccharomyces cerevisiae sensor</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2000-06-01</date><risdate>2000</risdate><volume>15</volume><issue>3</issue><spage>211</spage><epage>219</epage><pages>211-219</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>The first microbial biosensor to detect Cu
2+ by an amperometric method has been developed. For this purpose, recombinant
Saccharomyces cerevisiae strains are suitable as the microbial component. These strains contain plasmids with the Cu
2+-inducible promoter of the
CUP1-gene from
Saccharomyces cerevisiae fused to the
lacZ-gene from
E. coli. On this sensor the
CUP1 promoter is first induced by the Cu
2+-containing probe and subsequently lactose is used as a deputy substrate to make the measurement. If Cu
2+ is present in the sample, these recombinant strains are able to utilize lactose as a carbon source, which leads to alterations in the oxygen consumption of the cells. The sensor measured Cu
2+ in a concentration range between 0.5 and 2 mM CuSO
4. In addition, an indirect amperometric measurement principle was developed which allows the detection of samples containing Cu
2+ and fast biodegradable substances.</abstract><cop>Lausanne</cop><pub>Elsevier B.V</pub><pmid>11286339</pmid><doi>10.1016/S0956-5663(00)00060-9</doi><tpages>9</tpages></addata></record> |
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| subjects | Base Sequence Biological and medical sciences Biosensing Techniques - methods Biosensors Biotechnology Carrier Proteins Cells, Immobilized Copper - analysis CUP1 promoter Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genes, Fungal Heavy metal ions Lac Operon Lactose - metabolism lacZ-gene Metallothionein - genetics Metallothionein - metabolism Methods. Procedures. Technologies Microbial sensor Microscopy, Electron, Scanning Plasmids - genetics Promoter Regions, Genetic Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomycescerevisiae Substrate Specificity Various methods and equipments |
| title | Amperometric measurement of copper ions with a deputy substrate using a novel Saccharomyces cerevisiae sensor |
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