Skeletal muscle relaxation with diazo-2: The effect of altered pH
In a fatigued muscle fibre, the concentrations of ADP, P i and H + are all increased and relaxation is slowed. We have used the technique of laser flash photolysis of the caged calcium-chelator, diazo-2, to investigate the direct effect of changes in pH (pH 6.5, 7.0, 7.5) upon tension during relaxat...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1991-12, Vol.181 (3), p.1337-1342 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Palmer, R.E. Simnett, S.J. Mulligan, I.P. Ashley, C.C. |
| description | In a fatigued muscle fibre, the concentrations of ADP, P
i and H
+ are all increased and relaxation is slowed. We have used the technique of laser flash photolysis of the caged calcium-chelator, diazo-2, to investigate the direct effect of changes in pH (pH 6.5, 7.0, 7.5) upon tension during relaxation of single chemically skinned fibres, when the effects of the sarcoplasmic reticulum are absent. The relaxation transients were closely fitted with 2 exponentials, a fast (42.3 ± 1.4; pH 7.0) and a slow process (12.0 ± 0.7; pH 7.0). The fast phase of relaxation was pH sensitive; lowering pH leading to a slowing of the rate of force decline and raising pH leading to an increase of the rate. The rate of the slow phase was unaltered by changing pH over the range investigated. Thus the slowing of relaxation in fatigued muscle may be due, in part, to the direct action of protons on the myofilaments independent of any effects upon the sarcoplasmic reticulum. |
| doi_str_mv | 10.1016/0006-291X(91)92085-X |
| format | Article |
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i and H
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i and H
+ are all increased and relaxation is slowed. We have used the technique of laser flash photolysis of the caged calcium-chelator, diazo-2, to investigate the direct effect of changes in pH (pH 6.5, 7.0, 7.5) upon tension during relaxation of single chemically skinned fibres, when the effects of the sarcoplasmic reticulum are absent. The relaxation transients were closely fitted with 2 exponentials, a fast (42.3 ± 1.4; pH 7.0) and a slow process (12.0 ± 0.7; pH 7.0). The fast phase of relaxation was pH sensitive; lowering pH leading to a slowing of the rate of force decline and raising pH leading to an increase of the rate. The rate of the slow phase was unaltered by changing pH over the range investigated. Thus the slowing of relaxation in fatigued muscle may be due, in part, to the direct action of protons on the myofilaments independent of any effects upon the sarcoplasmic reticulum.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell physiology</subject><subject>Chelating Agents - pharmacology</subject><subject>Diazonium Compounds</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen-Ion Concentration</subject><subject>In Vitro Techniques</subject><subject>Molecular and cellular biology</subject><subject>Muscle contraction</subject><subject>Muscle Relaxation - drug effects</subject><subject>Muscles - drug effects</subject><subject>Muscles - physiology</subject><subject>Phenoxyacetates</subject><subject>Photolysis</subject><subject>Rana temporaria</subject><subject>Time Factors</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1vEzEQhq0KVELpP2glH1AFhy1jr-2se6hUVUCRKvVAkXKzZu2xanCywd7w9evZkKjcQHOYwzzvzOhh7ETAuQBh3gCAaaQVi1dWvLYSOt0sDthMgIVGClBP2OwRecae1_oZQAhl7CE7FHOjoFMzdvXxC2UaMfPlpvpMvFDGHzimYcW_p_GBh4S_hkZe8PsH4hQj-ZEPkWMeqVDg65sX7GnEXOl434_Yp3dv769vmtu79x-ur24br0w3Nl2LHRpvscVWiy7EFtCCDL1uvTDGQPCEoRN9D6pV0qOOOmDf69ibXmnVHrGz3d51Gb5uqI5umaqnnHFFw6a6udR2rufmv6AwU0nZTaDagb4MtRaKbl3SEstPJ8BtFbutP7f156xwfxS7xRQ73e_f9EsKf0M7p9P85X6O1WOOBVc-1UdMCzBSb69f7jCapH1LVFz1iVaeQiqTZBeG9O8_fgMe7pbY</recordid><startdate>19911231</startdate><enddate>19911231</enddate><creator>Palmer, R.E.</creator><creator>Simnett, S.J.</creator><creator>Mulligan, I.P.</creator><creator>Ashley, C.C.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19911231</creationdate><title>Skeletal muscle relaxation with diazo-2: The effect of altered pH</title><author>Palmer, R.E. ; Simnett, S.J. ; Mulligan, I.P. ; Ashley, C.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c468t-83a8a6c9a3a3518df30a902db53c16660dcead81bb04342ca5f5dabb5fb6b4543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell physiology</topic><topic>Chelating Agents - pharmacology</topic><topic>Diazonium Compounds</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrogen-Ion Concentration</topic><topic>In Vitro Techniques</topic><topic>Molecular and cellular biology</topic><topic>Muscle contraction</topic><topic>Muscle Relaxation - drug effects</topic><topic>Muscles - drug effects</topic><topic>Muscles - physiology</topic><topic>Phenoxyacetates</topic><topic>Photolysis</topic><topic>Rana temporaria</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Palmer, R.E.</creatorcontrib><creatorcontrib>Simnett, S.J.</creatorcontrib><creatorcontrib>Mulligan, I.P.</creatorcontrib><creatorcontrib>Ashley, C.C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Palmer, R.E.</au><au>Simnett, S.J.</au><au>Mulligan, I.P.</au><au>Ashley, C.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Skeletal muscle relaxation with diazo-2: The effect of altered pH</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1991-12-31</date><risdate>1991</risdate><volume>181</volume><issue>3</issue><spage>1337</spage><epage>1342</epage><pages>1337-1342</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>In a fatigued muscle fibre, the concentrations of ADP, P
i and H
+ are all increased and relaxation is slowed. We have used the technique of laser flash photolysis of the caged calcium-chelator, diazo-2, to investigate the direct effect of changes in pH (pH 6.5, 7.0, 7.5) upon tension during relaxation of single chemically skinned fibres, when the effects of the sarcoplasmic reticulum are absent. The relaxation transients were closely fitted with 2 exponentials, a fast (42.3 ± 1.4; pH 7.0) and a slow process (12.0 ± 0.7; pH 7.0). The fast phase of relaxation was pH sensitive; lowering pH leading to a slowing of the rate of force decline and raising pH leading to an increase of the rate. The rate of the slow phase was unaltered by changing pH over the range investigated. Thus the slowing of relaxation in fatigued muscle may be due, in part, to the direct action of protons on the myofilaments independent of any effects upon the sarcoplasmic reticulum.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1764084</pmid><doi>10.1016/0006-291X(91)92085-X</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1991-12, Vol.181 (3), p.1337-1342 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Biological and medical sciences Cell physiology Chelating Agents - pharmacology Diazonium Compounds Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration In Vitro Techniques Molecular and cellular biology Muscle contraction Muscle Relaxation - drug effects Muscles - drug effects Muscles - physiology Phenoxyacetates Photolysis Rana temporaria Time Factors |
| title | Skeletal muscle relaxation with diazo-2: The effect of altered pH |
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