A method for establishing primary cultures of human gastric epithelial cells
Long-term culture of human gastric epithelial cells has been difficult, and at present no normal human gastric epithelial cell lines are readily available. As part of our experiments to study pathogenesis of H. pylori, a bacterium that infects the stomach, we developed methods to culture normal huma...
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| Veröffentlicht in: | Methods in cell science 2000, Vol.22 (2-3), p.133-136 |
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| container_title | Methods in cell science |
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| creator | Smoot, D T Sewchand, J Young, K Desbordes, B C Allen, C R Naab, T |
| description | Long-term culture of human gastric epithelial cells has been difficult, and at present no normal human gastric epithelial cell lines are readily available. As part of our experiments to study pathogenesis of H. pylori, a bacterium that infects the stomach, we developed methods to culture normal human gastric epithelial cells. Primary cultures of human gastric epithelial cells can be established from gastric biopsies taken at upper G.I. endoscopy. Enzymatically isolated gastric epithelial-like cells are present in tight colonies on culture dishes within 24 hours of placing the cells in culture. Cells isolated stain positively for cytokeratin and produce neutral mucins, indicating that they are mucin secreting epithelial cells, consistent with gastric epithelial cells. Epithelial cells can be maintained up to 4 weeks in culture with evidence of DNA synthesis up through the first week of culture. |
| doi_str_mv | 10.1023/a:1009846624044 |
| format | Article |
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As part of our experiments to study pathogenesis of H. pylori, a bacterium that infects the stomach, we developed methods to culture normal human gastric epithelial cells. Primary cultures of human gastric epithelial cells can be established from gastric biopsies taken at upper G.I. endoscopy. Enzymatically isolated gastric epithelial-like cells are present in tight colonies on culture dishes within 24 hours of placing the cells in culture. Cells isolated stain positively for cytokeratin and produce neutral mucins, indicating that they are mucin secreting epithelial cells, consistent with gastric epithelial cells. 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As part of our experiments to study pathogenesis of H. pylori, a bacterium that infects the stomach, we developed methods to culture normal human gastric epithelial cells. Primary cultures of human gastric epithelial cells can be established from gastric biopsies taken at upper G.I. endoscopy. Enzymatically isolated gastric epithelial-like cells are present in tight colonies on culture dishes within 24 hours of placing the cells in culture. Cells isolated stain positively for cytokeratin and produce neutral mucins, indicating that they are mucin secreting epithelial cells, consistent with gastric epithelial cells. Epithelial cells can be maintained up to 4 weeks in culture with evidence of DNA synthesis up through the first week of culture.</description><subject>Biopsy</subject><subject>Cell Culture Techniques</subject><subject>Cell Separation</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelial Cells - microbiology</subject><subject>Gastric Mucosa - cytology</subject><subject>Gastric Mucosa - microbiology</subject><subject>Helicobacter pylori - cytology</subject><subject>Helicobacter pylori - pathogenicity</subject><subject>Humans</subject><subject>Keratins</subject><subject>Mucins</subject><issn>1381-5741</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1jz1PwzAURT2AaCnMbMgTW8DPfnZitqqigFSJpXvkrzRGThPiZODfU0SZ7nJ0dQ4hd8AegXHxZJ6BMV2hUhwZ4gVZgqigkCXCglzn_MkY41qqK7IA4Ao14pLs1rQLU9t72vQjDXkyNsXcxuOBDmPszPhN3ZymeQyZ9g1t584c6cHkaYyOhiFObUjRJOpCSvmGXDYm5XB73hXZb1_2m7di9_H6vlnvCscFToW3VlvjUJYCJFeGaWFAO-mELZUT2iE4ZcBLU6EHp6XVHsrmxHsG2osVefi7Hcb-az45113MvwLmGPo51yWXWgKWJ_D-DM62C74-F9X_-eIH6Sda5A</recordid><startdate>2000</startdate><enddate>2000</enddate><creator>Smoot, D T</creator><creator>Sewchand, J</creator><creator>Young, K</creator><creator>Desbordes, B C</creator><creator>Allen, C R</creator><creator>Naab, T</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>2000</creationdate><title>A method for establishing primary cultures of human gastric epithelial cells</title><author>Smoot, D T ; Sewchand, J ; Young, K ; Desbordes, B C ; Allen, C R ; Naab, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c234t-dbb9bac45731526a093a19c5c3b76c39c41c6a1d5a84d1c95b9d17f457d019d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Biopsy</topic><topic>Cell Culture Techniques</topic><topic>Cell Separation</topic><topic>Epithelial Cells - cytology</topic><topic>Epithelial Cells - microbiology</topic><topic>Gastric Mucosa - cytology</topic><topic>Gastric Mucosa - microbiology</topic><topic>Helicobacter pylori - cytology</topic><topic>Helicobacter pylori - pathogenicity</topic><topic>Humans</topic><topic>Keratins</topic><topic>Mucins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Smoot, D T</creatorcontrib><creatorcontrib>Sewchand, J</creatorcontrib><creatorcontrib>Young, K</creatorcontrib><creatorcontrib>Desbordes, B C</creatorcontrib><creatorcontrib>Allen, C R</creatorcontrib><creatorcontrib>Naab, T</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Methods in cell science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Smoot, D T</au><au>Sewchand, J</au><au>Young, K</au><au>Desbordes, B C</au><au>Allen, C R</au><au>Naab, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A method for establishing primary cultures of human gastric epithelial cells</atitle><jtitle>Methods in cell science</jtitle><addtitle>Methods Cell Sci</addtitle><date>2000</date><risdate>2000</risdate><volume>22</volume><issue>2-3</issue><spage>133</spage><epage>136</epage><pages>133-136</pages><issn>1381-5741</issn><abstract>Long-term culture of human gastric epithelial cells has been difficult, and at present no normal human gastric epithelial cell lines are readily available. As part of our experiments to study pathogenesis of H. pylori, a bacterium that infects the stomach, we developed methods to culture normal human gastric epithelial cells. Primary cultures of human gastric epithelial cells can be established from gastric biopsies taken at upper G.I. endoscopy. Enzymatically isolated gastric epithelial-like cells are present in tight colonies on culture dishes within 24 hours of placing the cells in culture. Cells isolated stain positively for cytokeratin and produce neutral mucins, indicating that they are mucin secreting epithelial cells, consistent with gastric epithelial cells. Epithelial cells can be maintained up to 4 weeks in culture with evidence of DNA synthesis up through the first week of culture.</abstract><cop>Netherlands</cop><pmid>11264944</pmid><doi>10.1023/a:1009846624044</doi><tpages>4</tpages></addata></record> |
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| identifier | ISSN: 1381-5741 |
| ispartof | Methods in cell science, 2000, Vol.22 (2-3), p.133-136 |
| issn | 1381-5741 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Biopsy Cell Culture Techniques Cell Separation Epithelial Cells - cytology Epithelial Cells - microbiology Gastric Mucosa - cytology Gastric Mucosa - microbiology Helicobacter pylori - cytology Helicobacter pylori - pathogenicity Humans Keratins Mucins |
| title | A method for establishing primary cultures of human gastric epithelial cells |
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