Kinetic study of a thermostable beta-glycosidase of Thermus thermophilus. Effects of temperature and glucose on hydrolysis and transglycosylation reactions

A beta-glycosidase of a thermophile, Thermus thermophilus, belonging to the glycoside hydrolase family 1, was cloned and overexpressed in Escherichia coli. The purified enzyme (Ttbetagly) has a broad substrate specificity towards beta-D-glucoside, beta-D-galactoside and beta-D-fucoside derivatives....

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Veröffentlicht in:	Glycoconjugate journal 2000-06, Vol.17 (6), p.377-383
Hauptverfasser:	Fourage, L, Dion, M, Colas, B
Format:	Artikel
Sprache:	eng
Schlagworte:	Enzyme Stability Glucose - pharmacology Glycoside Hydrolases - metabolism Glycosylation Hydrolysis Kinetics Substrate Specificity Temperature Thermus thermophilus - enzymology
Online-Zugang:	Volltext
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