An improved immunomagnetic procedure for the isolation of highly purified human blood eosinophils
A simple method is described for the isolation of human peripheral blood eosinophils by an immunomagnetic procedure. Superparamagnetic particles were coupled to a monoclonal antibody against CD16, a molecule present on neutrophils but not on eosinophils. A peripheral blood granulocyte preparation, c...
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| Veröffentlicht in: | Journal of immunological methods 1991-12, Vol.145 (1), p.105-110 |
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| container_title | Journal of immunological methods |
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| creator | Hansel, Trevor T. De Vries, I.Jolanda M. Iff, Thomas Rihs, Sylvia Wandzilak, Maciej Betz, Susanne Blaser, Kurt Walker, Christoph |
| description | A simple method is described for the isolation of human peripheral blood eosinophils by an immunomagnetic procedure. Superparamagnetic particles were coupled to a monoclonal antibody against CD16, a molecule present on neutrophils but not on eosinophils. A peripheral blood granulocyte preparation, containing neutrophils and eosinophils, was incubated with these anti-CD16 particles. In the magnetic field of a permanent magnet, magnetically labelled neutrophils were then retained on columns with a ferromagnetic matrix. By this negative selection procedure, eosinophils of 99.5% purity were obtained from normal individuals and 99.6% purity from patients with eczema. A comparison was made between the immunomagnetic method and eosinophil isolation after N-formyl-methionyl-leucyl-phenylalanine treatment. Even in the case of individuals with very low eosinophil counts, the immunomagnetic method permits the efficient isolation of highly purified and functionally active eosinophils. |
| doi_str_mv | 10.1016/0022-1759(91)90315-7 |
| format | Article |
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Superparamagnetic particles were coupled to a monoclonal antibody against CD16, a molecule present on neutrophils but not on eosinophils. A peripheral blood granulocyte preparation, containing neutrophils and eosinophils, was incubated with these anti-CD16 particles. In the magnetic field of a permanent magnet, magnetically labelled neutrophils were then retained on columns with a ferromagnetic matrix. By this negative selection procedure, eosinophils of 99.5% purity were obtained from normal individuals and 99.6% purity from patients with eczema. A comparison was made between the immunomagnetic method and eosinophil isolation after N-formyl-methionyl-leucyl-phenylalanine treatment. Even in the case of individuals with very low eosinophil counts, the immunomagnetic method permits the efficient isolation of highly purified and functionally active eosinophils.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/0022-1759(91)90315-7</identifier><identifier>PMID: 1662676</identifier><identifier>CODEN: JIMMBG</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Anti-CD16 ; Antigens, CD - analysis ; Antigens, Differentiation - immunology ; Biological and medical sciences ; Cell Separation - methods ; Eczema - physiopathology ; Eosinophil, human ; Eosinophils - cytology ; Eosinophils - immunology ; Eosinophils - physiology ; Formyl-methionyl-leucyl-phenylalanine ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Humans ; Immunomagnetic cell separation ; Magnetics ; Membrane Glycoproteins ; Molecular immunology ; N-Formylmethionine Leucyl-Phenylalanine - pharmacology ; Neutrophils - cytology ; Neutrophils - immunology ; Receptors, Fc - immunology ; Receptors, IgG ; Superoxides - metabolism ; Techniques ; Tetraspanin 29</subject><ispartof>Journal of immunological methods, 1991-12, Vol.145 (1), p.105-110</ispartof><rights>1991</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-a47346b8890b79efa5a66c1747893b3e09c4ffa41862d8e9e0db0375a47f4efa3</citedby><cites>FETCH-LOGICAL-c417t-a47346b8890b79efa5a66c1747893b3e09c4ffa41862d8e9e0db0375a47f4efa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0022-1759(91)90315-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5105987$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1662676$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hansel, Trevor T.</creatorcontrib><creatorcontrib>De Vries, I.Jolanda M.</creatorcontrib><creatorcontrib>Iff, Thomas</creatorcontrib><creatorcontrib>Rihs, Sylvia</creatorcontrib><creatorcontrib>Wandzilak, Maciej</creatorcontrib><creatorcontrib>Betz, Susanne</creatorcontrib><creatorcontrib>Blaser, Kurt</creatorcontrib><creatorcontrib>Walker, Christoph</creatorcontrib><title>An improved immunomagnetic procedure for the isolation of highly purified human blood eosinophils</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>A simple method is described for the isolation of human peripheral blood eosinophils by an immunomagnetic procedure. Superparamagnetic particles were coupled to a monoclonal antibody against CD16, a molecule present on neutrophils but not on eosinophils. A peripheral blood granulocyte preparation, containing neutrophils and eosinophils, was incubated with these anti-CD16 particles. In the magnetic field of a permanent magnet, magnetically labelled neutrophils were then retained on columns with a ferromagnetic matrix. By this negative selection procedure, eosinophils of 99.5% purity were obtained from normal individuals and 99.6% purity from patients with eczema. A comparison was made between the immunomagnetic method and eosinophil isolation after N-formyl-methionyl-leucyl-phenylalanine treatment. Even in the case of individuals with very low eosinophil counts, the immunomagnetic method permits the efficient isolation of highly purified and functionally active eosinophils.</description><subject>Anti-CD16</subject><subject>Antigens, CD - analysis</subject><subject>Antigens, Differentiation - immunology</subject><subject>Biological and medical sciences</subject><subject>Cell Separation - methods</subject><subject>Eczema - physiopathology</subject><subject>Eosinophil, human</subject><subject>Eosinophils - cytology</subject><subject>Eosinophils - immunology</subject><subject>Eosinophils - physiology</subject><subject>Formyl-methionyl-leucyl-phenylalanine</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Humans</subject><subject>Immunomagnetic cell separation</subject><subject>Magnetics</subject><subject>Membrane Glycoproteins</subject><subject>Molecular immunology</subject><subject>N-Formylmethionine Leucyl-Phenylalanine - pharmacology</subject><subject>Neutrophils - cytology</subject><subject>Neutrophils - immunology</subject><subject>Receptors, Fc - immunology</subject><subject>Receptors, IgG</subject><subject>Superoxides - metabolism</subject><subject>Techniques</subject><subject>Tetraspanin 29</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9rFTEUxYMo9bX6DRSyENHFaO5M_kw2hVKsCgU3ug6ZzE0nMpM8k5lCv715vkfd6SqX3N85XM4h5BWwD8BAfmSsbRtQQr_T8F6zDkSjnpAd9KptlGbiKdk9Is_JeSk_GWPAJDsjZyBlK5XcEXsVaVj2Od3jWIdli2mxdxHX4Gj9dThuGalPma4T0lDSbNeQIk2eTuFumh_ofsvBh6qetsVGOswpjRRTCTHtpzCXF-SZt3PBl6f3gvy4-fT9-ktz--3z1-ur28ZxUGtjueq4HPpes0Fp9FZYKR0ornrdDR0y7bj3lkMv27FHjWwcWKdE1Xle8e6CvD361rN_bVhWs4TicJ5txLQVo1qhW2Dtf0GQIJQCXkF-BF1OpWT0Zp_DYvODAWYOFZhDvuaQr9Fg_lRgVJW9Pvlvw4LjX9Ex87p_c9rb4uzss40ulEdMABO6P9hcHjGsod0HzKa4gLE2EjK61Ywp_PuO346hoyI</recordid><startdate>19911215</startdate><enddate>19911215</enddate><creator>Hansel, Trevor T.</creator><creator>De Vries, I.Jolanda M.</creator><creator>Iff, Thomas</creator><creator>Rihs, Sylvia</creator><creator>Wandzilak, Maciej</creator><creator>Betz, Susanne</creator><creator>Blaser, Kurt</creator><creator>Walker, Christoph</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19911215</creationdate><title>An improved immunomagnetic procedure for the isolation of highly purified human blood eosinophils</title><author>Hansel, Trevor T. ; De Vries, I.Jolanda M. ; Iff, Thomas ; Rihs, Sylvia ; Wandzilak, Maciej ; Betz, Susanne ; Blaser, Kurt ; Walker, Christoph</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-a47346b8890b79efa5a66c1747893b3e09c4ffa41862d8e9e0db0375a47f4efa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Anti-CD16</topic><topic>Antigens, CD - analysis</topic><topic>Antigens, Differentiation - immunology</topic><topic>Biological and medical sciences</topic><topic>Cell Separation - methods</topic><topic>Eczema - physiopathology</topic><topic>Eosinophil, human</topic><topic>Eosinophils - cytology</topic><topic>Eosinophils - immunology</topic><topic>Eosinophils - physiology</topic><topic>Formyl-methionyl-leucyl-phenylalanine</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Humans</topic><topic>Immunomagnetic cell separation</topic><topic>Magnetics</topic><topic>Membrane Glycoproteins</topic><topic>Molecular immunology</topic><topic>N-Formylmethionine Leucyl-Phenylalanine - pharmacology</topic><topic>Neutrophils - cytology</topic><topic>Neutrophils - immunology</topic><topic>Receptors, Fc - immunology</topic><topic>Receptors, IgG</topic><topic>Superoxides - metabolism</topic><topic>Techniques</topic><topic>Tetraspanin 29</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hansel, Trevor T.</creatorcontrib><creatorcontrib>De Vries, I.Jolanda M.</creatorcontrib><creatorcontrib>Iff, Thomas</creatorcontrib><creatorcontrib>Rihs, Sylvia</creatorcontrib><creatorcontrib>Wandzilak, Maciej</creatorcontrib><creatorcontrib>Betz, Susanne</creatorcontrib><creatorcontrib>Blaser, Kurt</creatorcontrib><creatorcontrib>Walker, Christoph</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hansel, Trevor T.</au><au>De Vries, I.Jolanda M.</au><au>Iff, Thomas</au><au>Rihs, Sylvia</au><au>Wandzilak, Maciej</au><au>Betz, Susanne</au><au>Blaser, Kurt</au><au>Walker, Christoph</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An improved immunomagnetic procedure for the isolation of highly purified human blood eosinophils</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>1991-12-15</date><risdate>1991</risdate><volume>145</volume><issue>1</issue><spage>105</spage><epage>110</epage><pages>105-110</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><coden>JIMMBG</coden><abstract>A simple method is described for the isolation of human peripheral blood eosinophils by an immunomagnetic procedure. Superparamagnetic particles were coupled to a monoclonal antibody against CD16, a molecule present on neutrophils but not on eosinophils. A peripheral blood granulocyte preparation, containing neutrophils and eosinophils, was incubated with these anti-CD16 particles. In the magnetic field of a permanent magnet, magnetically labelled neutrophils were then retained on columns with a ferromagnetic matrix. By this negative selection procedure, eosinophils of 99.5% purity were obtained from normal individuals and 99.6% purity from patients with eczema. A comparison was made between the immunomagnetic method and eosinophil isolation after N-formyl-methionyl-leucyl-phenylalanine treatment. Even in the case of individuals with very low eosinophil counts, the immunomagnetic method permits the efficient isolation of highly purified and functionally active eosinophils.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>1662676</pmid><doi>10.1016/0022-1759(91)90315-7</doi><tpages>6</tpages></addata></record> |
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| subjects | Anti-CD16 Antigens, CD - analysis Antigens, Differentiation - immunology Biological and medical sciences Cell Separation - methods Eczema - physiopathology Eosinophil, human Eosinophils - cytology Eosinophils - immunology Eosinophils - physiology Formyl-methionyl-leucyl-phenylalanine Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immunomagnetic cell separation Magnetics Membrane Glycoproteins Molecular immunology N-Formylmethionine Leucyl-Phenylalanine - pharmacology Neutrophils - cytology Neutrophils - immunology Receptors, Fc - immunology Receptors, IgG Superoxides - metabolism Techniques Tetraspanin 29 |
| title | An improved immunomagnetic procedure for the isolation of highly purified human blood eosinophils |
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