Inhibition of airway smooth muscle tone by Chinese herbal medicines
The aim of the present study was to elucidate whether Chinese traditional herbal drugs, Gorei‐San (TJ‐17) and Toki‐Shakuyaku‐San (TJ‐23), affect airway smooth muscle tone and, if so, to determine what the mechanism of action is. Rabbit tracheal segments were isolated and the contractile responses to...
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| creator | Tagaya, E Tamaoki, J Kawatani, K Taira, M Nagai, A |
| description | The aim of the present study was to elucidate whether Chinese traditional herbal drugs, Gorei‐San (TJ‐17) and Toki‐Shakuyaku‐San (TJ‐23), affect airway smooth muscle tone and, if so, to determine what the mechanism of action is.
Rabbit tracheal segments were isolated and the contractile responses to electrical field stimulation and acetylcholine were measured before and after the application of TJ‐17 or TJ‐23 under isometric conditions in vitro. Ouabain‐sensitive rubidium‐86 (86Rb) uptake by tissues in response to each drug was also measured.
Each herbal medicine attenuated the contractile responses to electrical field stimulation and acetylcholine in a concentration‐dependent manner, the maximal inhibition of acetylcholine‐induced contraction being 37.5±4.9% for TJ‐17 and 42.4±5.3% for TJ‐23 (p |
| doi_str_mv | 10.1034/j.1399-3003.2000.16f18.x |
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Rabbit tracheal segments were isolated and the contractile responses to electrical field stimulation and acetylcholine were measured before and after the application of TJ‐17 or TJ‐23 under isometric conditions in vitro. Ouabain‐sensitive rubidium‐86 (86Rb) uptake by tissues in response to each drug was also measured.
Each herbal medicine attenuated the contractile responses to electrical field stimulation and acetylcholine in a concentration‐dependent manner, the maximal inhibition of acetylcholine‐induced contraction being 37.5±4.9% for TJ‐17 and 42.4±5.3% for TJ‐23 (p<0.05 for each). These effects were not altered by mechanical removal of the epithelium, indomethacin, the nitric oxide synthase inhibitor NG‐nitro‐
l‐arginine methyl ester, the cyclic adenosine monophosphate (cAMP)‐dependent protein kinase inhibitor adenosine 3′5′‐cyclic monophosphorothiioate (Rp‐cAMPS), the cyclic guanosine monophosphate (cGMP)‐dependent protein kinase inhibitor KT5823, or the calcium (Ca2+)‐activated potassium (K+) channel inhibitor chary‐bdotoxin, but were greatly inhibited in the presence of the sodium (Na+)‐K+ adenosine triphosphatase (ATPase) inhibitor ouabain. Incubation of tissues with TJ‐17 and TJ‐23 dose dependently increased ouabain‐sensitive 86Rb uptake.
The results of the study suggest that both Gorei‐San and Toki‐Shakuyaku‐San reduce airway smooth muscle tone via a postjunctional mechanism probably through stimulation of the sodium pump and the subsequent hyperpolarization/repolarization of the cell membrane. These effects may contribute to the antiasthmatic properties of these herbal medicines.</description><identifier>ISSN: 0903-1936</identifier><identifier>EISSN: 1399-3003</identifier><identifier>DOI: 10.1034/j.1399-3003.2000.16f18.x</identifier><identifier>PMID: 11292117</identifier><language>eng</language><publisher>Sheffield: Eur Respiratory Soc</publisher><subject>Acetylcholine ; Acetylcholine - antagonists & inhibitors ; Acetylcholine - pharmacology ; Animals ; asthma ; Biological and medical sciences ; Bronchial Provocation Tests ; bronchoconstriction ; Bronchoconstriction - drug effects ; Culture Techniques ; Drugs, Chinese Herbal - pharmacology ; Female ; Male ; Medical sciences ; Medicine, Kampo ; Muscle, Smooth - drug effects ; ouabain ; Pharmacology. Drug treatments ; Rabbits ; Respiratory system ; sodium‐potassium adenosine triphosphate</subject><ispartof>The European respiratory journal, 2000-12, Vol.16 (6), p.1123-1128</ispartof><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5418-3b3749124b4822db5758b3242de7452aab07f02796d28dc73f07732f1bdba293</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1034%2Fj.1399-3003.2000.16f18.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=906054$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11292117$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tagaya, E</creatorcontrib><creatorcontrib>Tamaoki, J</creatorcontrib><creatorcontrib>Kawatani, K</creatorcontrib><creatorcontrib>Taira, M</creatorcontrib><creatorcontrib>Nagai, A</creatorcontrib><title>Inhibition of airway smooth muscle tone by Chinese herbal medicines</title><title>The European respiratory journal</title><addtitle>Eur Respir J</addtitle><description>The aim of the present study was to elucidate whether Chinese traditional herbal drugs, Gorei‐San (TJ‐17) and Toki‐Shakuyaku‐San (TJ‐23), affect airway smooth muscle tone and, if so, to determine what the mechanism of action is.
Rabbit tracheal segments were isolated and the contractile responses to electrical field stimulation and acetylcholine were measured before and after the application of TJ‐17 or TJ‐23 under isometric conditions in vitro. Ouabain‐sensitive rubidium‐86 (86Rb) uptake by tissues in response to each drug was also measured.
Each herbal medicine attenuated the contractile responses to electrical field stimulation and acetylcholine in a concentration‐dependent manner, the maximal inhibition of acetylcholine‐induced contraction being 37.5±4.9% for TJ‐17 and 42.4±5.3% for TJ‐23 (p<0.05 for each). These effects were not altered by mechanical removal of the epithelium, indomethacin, the nitric oxide synthase inhibitor NG‐nitro‐
l‐arginine methyl ester, the cyclic adenosine monophosphate (cAMP)‐dependent protein kinase inhibitor adenosine 3′5′‐cyclic monophosphorothiioate (Rp‐cAMPS), the cyclic guanosine monophosphate (cGMP)‐dependent protein kinase inhibitor KT5823, or the calcium (Ca2+)‐activated potassium (K+) channel inhibitor chary‐bdotoxin, but were greatly inhibited in the presence of the sodium (Na+)‐K+ adenosine triphosphatase (ATPase) inhibitor ouabain. Incubation of tissues with TJ‐17 and TJ‐23 dose dependently increased ouabain‐sensitive 86Rb uptake.
The results of the study suggest that both Gorei‐San and Toki‐Shakuyaku‐San reduce airway smooth muscle tone via a postjunctional mechanism probably through stimulation of the sodium pump and the subsequent hyperpolarization/repolarization of the cell membrane. These effects may contribute to the antiasthmatic properties of these herbal medicines.</description><subject>Acetylcholine</subject><subject>Acetylcholine - antagonists & inhibitors</subject><subject>Acetylcholine - pharmacology</subject><subject>Animals</subject><subject>asthma</subject><subject>Biological and medical sciences</subject><subject>Bronchial Provocation Tests</subject><subject>bronchoconstriction</subject><subject>Bronchoconstriction - drug effects</subject><subject>Culture Techniques</subject><subject>Drugs, Chinese Herbal - pharmacology</subject><subject>Female</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Medicine, Kampo</subject><subject>Muscle, Smooth - drug effects</subject><subject>ouabain</subject><subject>Pharmacology. Drug treatments</subject><subject>Rabbits</subject><subject>Respiratory system</subject><subject>sodium‐potassium adenosine triphosphate</subject><issn>0903-1936</issn><issn>1399-3003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkF1LwzAUhoMoOqd_QQKCd505SZo0N4IMPxkI4n1I2tRmpK0mG3P_3tYNvfUqcPKcc97zIISBzIAwfr2cAVMqY4SwGSVkqIoaitnXAZr8fhyiCVGEZaCYOEGnKS0JAcEZHKMTAKoogJyg-VPXeOtXvu9wX2Pj48ZscWr7ftXgdp3K4PCq7xy2WzxvfOeSw42L1gTcusqXY-UMHdUmJHe-f6fo7f7ubf6YLV4enua3i6zMORQZs0xyBZRbXlBa2VzmhWWU08pJnlNjLJE1oVKJihZVKVlNpGS0BltZQxWboqvd2I_Yf65dWunWp9KFYDrXr5OWNC-kUHwAix1Yxj6l6Gr9EX1r4lYD0aM_vdSjJj1q0qM__eNPfw2tF_sdazvc99e4FzYAl3vApNKEOpqu9OmXU0SQfExws6M2Prjtv9fru9dnEPdQ_N3a-Pdm46PTqTUhDKlAu7gEocWYibFvTJqXbQ</recordid><startdate>200012</startdate><enddate>200012</enddate><creator>Tagaya, E</creator><creator>Tamaoki, J</creator><creator>Kawatani, K</creator><creator>Taira, M</creator><creator>Nagai, A</creator><general>Eur Respiratory Soc</general><general>Munksgaard International Publishers</general><general>Maney</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200012</creationdate><title>Inhibition of airway smooth muscle tone by Chinese herbal medicines</title><author>Tagaya, E ; Tamaoki, J ; Kawatani, K ; Taira, M ; Nagai, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5418-3b3749124b4822db5758b3242de7452aab07f02796d28dc73f07732f1bdba293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Acetylcholine</topic><topic>Acetylcholine - antagonists & inhibitors</topic><topic>Acetylcholine - pharmacology</topic><topic>Animals</topic><topic>asthma</topic><topic>Biological and medical sciences</topic><topic>Bronchial Provocation Tests</topic><topic>bronchoconstriction</topic><topic>Bronchoconstriction - drug effects</topic><topic>Culture Techniques</topic><topic>Drugs, Chinese Herbal - pharmacology</topic><topic>Female</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Medicine, Kampo</topic><topic>Muscle, Smooth - drug effects</topic><topic>ouabain</topic><topic>Pharmacology. Drug treatments</topic><topic>Rabbits</topic><topic>Respiratory system</topic><topic>sodium‐potassium adenosine triphosphate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tagaya, E</creatorcontrib><creatorcontrib>Tamaoki, J</creatorcontrib><creatorcontrib>Kawatani, K</creatorcontrib><creatorcontrib>Taira, M</creatorcontrib><creatorcontrib>Nagai, A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The European respiratory journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tagaya, E</au><au>Tamaoki, J</au><au>Kawatani, K</au><au>Taira, M</au><au>Nagai, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of airway smooth muscle tone by Chinese herbal medicines</atitle><jtitle>The European respiratory journal</jtitle><addtitle>Eur Respir J</addtitle><date>2000-12</date><risdate>2000</risdate><volume>16</volume><issue>6</issue><spage>1123</spage><epage>1128</epage><pages>1123-1128</pages><issn>0903-1936</issn><eissn>1399-3003</eissn><abstract>The aim of the present study was to elucidate whether Chinese traditional herbal drugs, Gorei‐San (TJ‐17) and Toki‐Shakuyaku‐San (TJ‐23), affect airway smooth muscle tone and, if so, to determine what the mechanism of action is.
Rabbit tracheal segments were isolated and the contractile responses to electrical field stimulation and acetylcholine were measured before and after the application of TJ‐17 or TJ‐23 under isometric conditions in vitro. Ouabain‐sensitive rubidium‐86 (86Rb) uptake by tissues in response to each drug was also measured.
Each herbal medicine attenuated the contractile responses to electrical field stimulation and acetylcholine in a concentration‐dependent manner, the maximal inhibition of acetylcholine‐induced contraction being 37.5±4.9% for TJ‐17 and 42.4±5.3% for TJ‐23 (p<0.05 for each). These effects were not altered by mechanical removal of the epithelium, indomethacin, the nitric oxide synthase inhibitor NG‐nitro‐
l‐arginine methyl ester, the cyclic adenosine monophosphate (cAMP)‐dependent protein kinase inhibitor adenosine 3′5′‐cyclic monophosphorothiioate (Rp‐cAMPS), the cyclic guanosine monophosphate (cGMP)‐dependent protein kinase inhibitor KT5823, or the calcium (Ca2+)‐activated potassium (K+) channel inhibitor chary‐bdotoxin, but were greatly inhibited in the presence of the sodium (Na+)‐K+ adenosine triphosphatase (ATPase) inhibitor ouabain. Incubation of tissues with TJ‐17 and TJ‐23 dose dependently increased ouabain‐sensitive 86Rb uptake.
The results of the study suggest that both Gorei‐San and Toki‐Shakuyaku‐San reduce airway smooth muscle tone via a postjunctional mechanism probably through stimulation of the sodium pump and the subsequent hyperpolarization/repolarization of the cell membrane. These effects may contribute to the antiasthmatic properties of these herbal medicines.</abstract><cop>Sheffield</cop><pub>Eur Respiratory Soc</pub><pmid>11292117</pmid><doi>10.1034/j.1399-3003.2000.16f18.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acetylcholine Acetylcholine - antagonists & inhibitors Acetylcholine - pharmacology Animals asthma Biological and medical sciences Bronchial Provocation Tests bronchoconstriction Bronchoconstriction - drug effects Culture Techniques Drugs, Chinese Herbal - pharmacology Female Male Medical sciences Medicine, Kampo Muscle, Smooth - drug effects ouabain Pharmacology. Drug treatments Rabbits Respiratory system sodium‐potassium adenosine triphosphate |
| title | Inhibition of airway smooth muscle tone by Chinese herbal medicines |
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